Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)

Autores
Baldessari, Jorge Javier
Año de publicación
2008
Idioma
inglés
Tipo de recurso
tesis doctoral
Estado
versión aceptada
Colaborador/a o director/a de tesis
Tillman, Barry L. (Director)
Descripción
Tesis para obtener el grado de Doctor en Filosofía, de la Universidad de Florida (Estados Unidos), en agosto de 2008
Tomato spotted wilt virus (TSWV, Bunyaviridae:Tospovirus) is a major peanut pathogen in the USA. Its management involves, among other factors, the use of resistant cultivars and recommended planting dates. Ten genotypes with varied degrees of resistance were field tested in two locations and four planting dates with the following objectives: 1) to ascertain the importance of planting dates and location as determining factors of spotted wilt epidemic intensity, 2) t evaluate the consistency in the performance of an array of genotypes with contrasting spotted wilt resistance assessed at different times, and 3) to provide an estimation of how much genotypic consistency can be ascribed to genetic causes. Results indicated that location was a significant factor in determining the spotted wilt damage, while planting date was significant only under a light epidemic or late in the season under a heavy epidemic. The high correlation between assessment dates implied that genotypic performance was perceived early and differences persisted until harvest. High Type B genetic correlation and repeatability suggested a strong genetic determination of resistance. Heritability is a genetic parameter of paramount importance for efficient plant breeding but no estimates have been published for resistance to TSWV in peanuts. To provide such estimates and assess resistance sources, five populations from three resistant and a susceptible parent were field tested in five environments in Florida, USA. Approximately 36,300 total plants were individually assessed three times for five spotted wilt symptoms using a six level scale. Each environment was individually analyzed using an Animal Model containing block, plot, additive and non-additive terms. High phenotypic (0.80-0.93) and genetic (0.88-0.99) correlation estimates between stunting and spots/mosaic were obtained. Individual-basis heritability estimates showed a wide range (0.01-0.71) although values most frequently were in the lowmedium range. This suggests individual selection for resistance to spotted wilt should not be applied in early generations within the tested populations. The resistant parents produced populations with similar breeding values when crossed to the susceptible parent, while the population from a cross between resistant parents exhibited the best breeding values for resistance to spotted wilt. A published inoculation method was used to study if inoculum age, viral concentration, and extent of rubbing during inoculation affected the frequency of infection. Results showed that neither number of rubbings nor inoculum concentration were important factors. Inoculum showed better infectivity 10 minutes after preparation than at zero or twenty minutes after preparation. Inoculum batch was an important factor; highlighting the fact that viral titer is highly variable even when collected from similar plant tissues. The overall low infection rates suggest that additional work is necessary for mechanical inoculation to be a reliable research tool.
EEA Manfredi
Fil: Baldessari, Jorge Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Materia
Virus del Bronceado del Tomate
Genética
Cacahuete
Arachis hypogaea
Característica del Sitio
Fecha de Plantación
Herencia Genética
Inoculación
Tomato Spotted Wilt Virus
Genetics
Groundnuts
Site Factors
Planting Date
Genetic Inheritance
Inoculation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26648
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oai_identifier_str oai:localhost:20.500.12123/26648
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spelling Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)Baldessari, Jorge JavierVirus del Bronceado del TomateGenéticaCacahueteArachis hypogaeaCaracterística del SitioFecha de PlantaciónHerencia GenéticaInoculaciónTomato Spotted Wilt VirusGeneticsGroundnutsSite FactorsPlanting DateGenetic InheritanceInoculationTesis para obtener el grado de Doctor en Filosofía, de la Universidad de Florida (Estados Unidos), en agosto de 2008Tomato spotted wilt virus (TSWV, Bunyaviridae:Tospovirus) is a major peanut pathogen in the USA. Its management involves, among other factors, the use of resistant cultivars and recommended planting dates. Ten genotypes with varied degrees of resistance were field tested in two locations and four planting dates with the following objectives: 1) to ascertain the importance of planting dates and location as determining factors of spotted wilt epidemic intensity, 2) t evaluate the consistency in the performance of an array of genotypes with contrasting spotted wilt resistance assessed at different times, and 3) to provide an estimation of how much genotypic consistency can be ascribed to genetic causes. Results indicated that location was a significant factor in determining the spotted wilt damage, while planting date was significant only under a light epidemic or late in the season under a heavy epidemic. The high correlation between assessment dates implied that genotypic performance was perceived early and differences persisted until harvest. High Type B genetic correlation and repeatability suggested a strong genetic determination of resistance. Heritability is a genetic parameter of paramount importance for efficient plant breeding but no estimates have been published for resistance to TSWV in peanuts. To provide such estimates and assess resistance sources, five populations from three resistant and a susceptible parent were field tested in five environments in Florida, USA. Approximately 36,300 total plants were individually assessed three times for five spotted wilt symptoms using a six level scale. Each environment was individually analyzed using an Animal Model containing block, plot, additive and non-additive terms. High phenotypic (0.80-0.93) and genetic (0.88-0.99) correlation estimates between stunting and spots/mosaic were obtained. Individual-basis heritability estimates showed a wide range (0.01-0.71) although values most frequently were in the lowmedium range. This suggests individual selection for resistance to spotted wilt should not be applied in early generations within the tested populations. The resistant parents produced populations with similar breeding values when crossed to the susceptible parent, while the population from a cross between resistant parents exhibited the best breeding values for resistance to spotted wilt. A published inoculation method was used to study if inoculum age, viral concentration, and extent of rubbing during inoculation affected the frequency of infection. Results showed that neither number of rubbings nor inoculum concentration were important factors. Inoculum showed better infectivity 10 minutes after preparation than at zero or twenty minutes after preparation. Inoculum batch was an important factor; highlighting the fact that viral titer is highly variable even when collected from similar plant tissues. The overall low infection rates suggest that additional work is necessary for mechanical inoculation to be a reliable research tool.EEA ManfrediFil: Baldessari, Jorge Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaUniversidad de FloridaTillman, Barry L. (Director)2026-06-16T17:39:45Z2026-06-16T17:39:45Z2008-08info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06info:ar-repo/semantics/tesisDoctoralapplication/pdfhttp://hdl.handle.net/20.500.12123/26648https://ufdc.ufl.edu/ufe0022367/00001engFlorida .......... (state) (World, North and Central America, United States)7007240info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuaria2026-09-24T11:43:20Zoai:localhost:20.500.12123/26648instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:22.184INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)
title Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)
spellingShingle Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)
Baldessari, Jorge Javier
Virus del Bronceado del Tomate
Genética
Cacahuete
Arachis hypogaea
Característica del Sitio
Fecha de Plantación
Herencia Genética
Inoculación
Tomato Spotted Wilt Virus
Genetics
Groundnuts
Site Factors
Planting Date
Genetic Inheritance
Inoculation
title_short Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)
title_full Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)
title_fullStr Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)
title_full_unstemmed Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)
title_sort Genetics of tomato spotted wilt virus resistance in peanut (Arachis hypogaea L.)
dc.creator.none.fl_str_mv Baldessari, Jorge Javier
author Baldessari, Jorge Javier
author_facet Baldessari, Jorge Javier
author_role author
dc.contributor.none.fl_str_mv Tillman, Barry L. (Director)
dc.subject.none.fl_str_mv Virus del Bronceado del Tomate
Genética
Cacahuete
Arachis hypogaea
Característica del Sitio
Fecha de Plantación
Herencia Genética
Inoculación
Tomato Spotted Wilt Virus
Genetics
Groundnuts
Site Factors
Planting Date
Genetic Inheritance
Inoculation
topic Virus del Bronceado del Tomate
Genética
Cacahuete
Arachis hypogaea
Característica del Sitio
Fecha de Plantación
Herencia Genética
Inoculación
Tomato Spotted Wilt Virus
Genetics
Groundnuts
Site Factors
Planting Date
Genetic Inheritance
Inoculation
dc.description.none.fl_txt_mv Tesis para obtener el grado de Doctor en Filosofía, de la Universidad de Florida (Estados Unidos), en agosto de 2008
Tomato spotted wilt virus (TSWV, Bunyaviridae:Tospovirus) is a major peanut pathogen in the USA. Its management involves, among other factors, the use of resistant cultivars and recommended planting dates. Ten genotypes with varied degrees of resistance were field tested in two locations and four planting dates with the following objectives: 1) to ascertain the importance of planting dates and location as determining factors of spotted wilt epidemic intensity, 2) t evaluate the consistency in the performance of an array of genotypes with contrasting spotted wilt resistance assessed at different times, and 3) to provide an estimation of how much genotypic consistency can be ascribed to genetic causes. Results indicated that location was a significant factor in determining the spotted wilt damage, while planting date was significant only under a light epidemic or late in the season under a heavy epidemic. The high correlation between assessment dates implied that genotypic performance was perceived early and differences persisted until harvest. High Type B genetic correlation and repeatability suggested a strong genetic determination of resistance. Heritability is a genetic parameter of paramount importance for efficient plant breeding but no estimates have been published for resistance to TSWV in peanuts. To provide such estimates and assess resistance sources, five populations from three resistant and a susceptible parent were field tested in five environments in Florida, USA. Approximately 36,300 total plants were individually assessed three times for five spotted wilt symptoms using a six level scale. Each environment was individually analyzed using an Animal Model containing block, plot, additive and non-additive terms. High phenotypic (0.80-0.93) and genetic (0.88-0.99) correlation estimates between stunting and spots/mosaic were obtained. Individual-basis heritability estimates showed a wide range (0.01-0.71) although values most frequently were in the lowmedium range. This suggests individual selection for resistance to spotted wilt should not be applied in early generations within the tested populations. The resistant parents produced populations with similar breeding values when crossed to the susceptible parent, while the population from a cross between resistant parents exhibited the best breeding values for resistance to spotted wilt. A published inoculation method was used to study if inoculum age, viral concentration, and extent of rubbing during inoculation affected the frequency of infection. Results showed that neither number of rubbings nor inoculum concentration were important factors. Inoculum showed better infectivity 10 minutes after preparation than at zero or twenty minutes after preparation. Inoculum batch was an important factor; highlighting the fact that viral titer is highly variable even when collected from similar plant tissues. The overall low infection rates suggest that additional work is necessary for mechanical inoculation to be a reliable research tool.
EEA Manfredi
Fil: Baldessari, Jorge Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
description Tesis para obtener el grado de Doctor en Filosofía, de la Universidad de Florida (Estados Unidos), en agosto de 2008
publishDate 2008
dc.date.none.fl_str_mv 2008-08
2026-06-16T17:39:45Z
2026-06-16T17:39:45Z
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_db06
info:ar-repo/semantics/tesisDoctoral
format doctoralThesis
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26648
https://ufdc.ufl.edu/ufe0022367/00001
url http://hdl.handle.net/20.500.12123/26648
https://ufdc.ufl.edu/ufe0022367/00001
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Florida .......... (state) (World, North and Central America, United States)
7007240
dc.publisher.none.fl_str_mv Universidad de Florida
publisher.none.fl_str_mv Universidad de Florida
dc.source.none.fl_str_mv reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.754232

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