Isolation of equine herpesvirus–2 from the lung of an aborted fetus

Autores
Galosi, Cecilia Mónica; Cid de la Paz, Viviana; Fernandez, Leandro Carlos; Martinez, Pablo Javier; Craig, María Isabel; Barrandeguy, Maria Edith; Etcheverrigaray, Maria Elisa
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study describes the isolation of equine herpesvirus–2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti–EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause–effect relationship was established between the EHV-2 isolate and the abortion.
Instituto de Virología
Fil: Galosi, Cecilia Mónica. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
Fil: Galosi, Cecilia Mónica. Buenos Aires (provincia). Comisión de Investigaciones Científicas; Argentina
Fil: Cid de la Paz, Viviana. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
Fil: Cid de la Paz, Viviana. Buenos Aires (provincia). Comisión de Investigaciones Científicas; Argentina
Fil: Fernandez, Leandro Carlos. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
Fil: Martinez, Carlos Javier. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina; Argentina
Fil: Etcheverrigaray, Maria Elisa. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
Fuente
Journal of Veterinary Diagnostic Investigation 17 : 500-502 (2005)
Materia
Isolation
Equine Herpesvirus
Abortion
Aislamiento
Herpes Virus Equino
Aborto
EHV-2
Aborted Fetus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Isolation of equine herpesvirus–2 from the lung of an aborted fetusGalosi, Cecilia MónicaCid de la Paz, VivianaFernandez, Leandro CarlosMartinez, Pablo JavierCraig, María IsabelBarrandeguy, Maria EdithEtcheverrigaray, Maria ElisaIsolationEquine HerpesvirusAbortionAislamientoHerpes Virus EquinoAbortoEHV-2Aborted FetusThis study describes the isolation of equine herpesvirus–2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti–EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause–effect relationship was established between the EHV-2 isolate and the abortion.Instituto de VirologíaFil: Galosi, Cecilia Mónica. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; ArgentinaFil: Galosi, Cecilia Mónica. Buenos Aires (provincia). Comisión de Investigaciones Científicas; ArgentinaFil: Cid de la Paz, Viviana. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; ArgentinaFil: Cid de la Paz, Viviana. Buenos Aires (provincia). Comisión de Investigaciones Científicas; ArgentinaFil: Fernandez, Leandro Carlos. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; ArgentinaFil: Martinez, Carlos Javier. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; ArgentinaFil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina; ArgentinaFil: Etcheverrigaray, Maria Elisa. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; ArgentinaSage Publications2026-07-29T14:00:14Z2026-07-29T14:00:14Z2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27190https://journals.sagepub.com/doi/10.1177/1040638705017005201943-49361040-6387https://doi.org/10.1177/104063870501700520Journal of Veterinary Diagnostic Investigation 17 : 500-502 (2005)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:36Zoai:localhost:20.500.12123/27190instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:37.071INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Isolation of equine herpesvirus–2 from the lung of an aborted fetus
title Isolation of equine herpesvirus–2 from the lung of an aborted fetus
spellingShingle Isolation of equine herpesvirus–2 from the lung of an aborted fetus
Galosi, Cecilia Mónica
Isolation
Equine Herpesvirus
Abortion
Aislamiento
Herpes Virus Equino
Aborto
EHV-2
Aborted Fetus
title_short Isolation of equine herpesvirus–2 from the lung of an aborted fetus
title_full Isolation of equine herpesvirus–2 from the lung of an aborted fetus
title_fullStr Isolation of equine herpesvirus–2 from the lung of an aborted fetus
title_full_unstemmed Isolation of equine herpesvirus–2 from the lung of an aborted fetus
title_sort Isolation of equine herpesvirus–2 from the lung of an aborted fetus
dc.creator.none.fl_str_mv Galosi, Cecilia Mónica
Cid de la Paz, Viviana
Fernandez, Leandro Carlos
Martinez, Pablo Javier
Craig, María Isabel
Barrandeguy, Maria Edith
Etcheverrigaray, Maria Elisa
author Galosi, Cecilia Mónica
author_facet Galosi, Cecilia Mónica
Cid de la Paz, Viviana
Fernandez, Leandro Carlos
Martinez, Pablo Javier
Craig, María Isabel
Barrandeguy, Maria Edith
Etcheverrigaray, Maria Elisa
author_role author
author2 Cid de la Paz, Viviana
Fernandez, Leandro Carlos
Martinez, Pablo Javier
Craig, María Isabel
Barrandeguy, Maria Edith
Etcheverrigaray, Maria Elisa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Isolation
Equine Herpesvirus
Abortion
Aislamiento
Herpes Virus Equino
Aborto
EHV-2
Aborted Fetus
topic Isolation
Equine Herpesvirus
Abortion
Aislamiento
Herpes Virus Equino
Aborto
EHV-2
Aborted Fetus
dc.description.none.fl_txt_mv This study describes the isolation of equine herpesvirus–2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti–EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause–effect relationship was established between the EHV-2 isolate and the abortion.
Instituto de Virología
Fil: Galosi, Cecilia Mónica. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
Fil: Galosi, Cecilia Mónica. Buenos Aires (provincia). Comisión de Investigaciones Científicas; Argentina
Fil: Cid de la Paz, Viviana. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
Fil: Cid de la Paz, Viviana. Buenos Aires (provincia). Comisión de Investigaciones Científicas; Argentina
Fil: Fernandez, Leandro Carlos. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
Fil: Martinez, Carlos Javier. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina; Argentina
Fil: Etcheverrigaray, Maria Elisa. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Veterinarias. Departamento de Virología; Argentina
description This study describes the isolation of equine herpesvirus–2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti–EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause–effect relationship was established between the EHV-2 isolate and the abortion.
publishDate 2005
dc.date.none.fl_str_mv 2005
2026-07-29T14:00:14Z
2026-07-29T14:00:14Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27190
https://journals.sagepub.com/doi/10.1177/104063870501700520
1943-4936
1040-6387
https://doi.org/10.1177/104063870501700520
url http://hdl.handle.net/20.500.12123/27190
https://journals.sagepub.com/doi/10.1177/104063870501700520
https://doi.org/10.1177/104063870501700520
identifier_str_mv 1943-4936
1040-6387
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sage Publications
publisher.none.fl_str_mv Sage Publications
dc.source.none.fl_str_mv Journal of Veterinary Diagnostic Investigation 17 : 500-502 (2005)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.733355

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