Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in Argentina

Autores
Hoffmann, Teresa Magali; Soria, Mario; Alcain, Andrea; Moroni, Mirian; Viñas, María Rosa; Tellez-Isaias, Guillermo; Bueno, Dante Javier
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
National poultry improvement programs utilize multistep isolation procedures for Salmonella spp. detection in poultry litter, including selective enrichment and plating on selective-differential solid media. This study assessed the prevalence and serovar distribution of Salmonella spp. in 1,004 naturally contaminated poultry litter swab samples collected across Argentina. Samples were obtained from 775 poultry farms, comprising broiler (n = 801), laying hen (n = 2), and broiler-breeder (n = 201) houses. The probability of Salmonella isolation was evaluated based on the diameter of migration zones on modified semisolid Rappaport-Vassiliadis (MSRV) agar. The selective and differential capacities of two selective-differential plating media, xylose lysine deoxycholate (XLD) agar and Salmonella-Shigella (SS) agar, were compared. The apparent prevalence of Salmonella spp. was 20.6%. The most frequently identified serovars included Salmonella ser. Enteritidis (42%), S. ser. Westhampton (13%), and S. ser. Newport (6%). An MSRV halo diameter of 33 mm was determined to be the optimal cut-off value for a high probability of Salmonella spp. isolation. No significant differences were observed between XLD and SS agars regarding selective capacity; however, XLD agar exhibited superior differential performance. The detection of S. ser. Enteritidis in poultry litter underscores the public health significance of this serovar in Argentine poultry production systems. In summary, XLD agar is superior to SS agar for colony differentiation, and MSRV halo diameters of 33 mm or greater are strongly associated with successful Salmonella spp. isolation from poultry litter swab samples.
EEA Concepción del Uruguay
Fil: Hoffmann, Teresa Magali. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hoffmann, Teresa Magali. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Alcain, Andrea. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Instituto Nacional de Enfermedades Infecciosas. Servicios de Enterobacterias; Argentina
Fil: Moroni, Mirian. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Instituto Nacional de Enfermedades Infecciosas. Servicios de Enterobacterias; Argentina
Fil: Viñas, María Rosa. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Instituto Nacional de Enfermedades Infecciosas. Servicios de Enterobacterias; Argentina
Fil: Tellez-Isaias, Guillermo. Gut Health LLC; Estados Unidos
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología. Sede Basavilbaso; Argentina
Fuente
German Journal of Veterinary Research 6 (2) : 124-136. (2026)
Materia
Aves de Corral
Enfermedades de los Animales
Cama (animal)
Argentina
Poultry
Salmonella
Animal Diseases
Litter for Animals
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26730
Acceder
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oai_identifier_str oai:localhost:20.500.12123/26730
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in ArgentinaHoffmann, Teresa MagaliSoria, MarioAlcain, AndreaMoroni, MirianViñas, María RosaTellez-Isaias, GuillermoBueno, Dante JavierAves de CorralEnfermedades de los AnimalesCama (animal)ArgentinaPoultrySalmonellaAnimal DiseasesLitter for AnimalsNational poultry improvement programs utilize multistep isolation procedures for Salmonella spp. detection in poultry litter, including selective enrichment and plating on selective-differential solid media. This study assessed the prevalence and serovar distribution of Salmonella spp. in 1,004 naturally contaminated poultry litter swab samples collected across Argentina. Samples were obtained from 775 poultry farms, comprising broiler (n = 801), laying hen (n = 2), and broiler-breeder (n = 201) houses. The probability of Salmonella isolation was evaluated based on the diameter of migration zones on modified semisolid Rappaport-Vassiliadis (MSRV) agar. The selective and differential capacities of two selective-differential plating media, xylose lysine deoxycholate (XLD) agar and Salmonella-Shigella (SS) agar, were compared. The apparent prevalence of Salmonella spp. was 20.6%. The most frequently identified serovars included Salmonella ser. Enteritidis (42%), S. ser. Westhampton (13%), and S. ser. Newport (6%). An MSRV halo diameter of 33 mm was determined to be the optimal cut-off value for a high probability of Salmonella spp. isolation. No significant differences were observed between XLD and SS agars regarding selective capacity; however, XLD agar exhibited superior differential performance. The detection of S. ser. Enteritidis in poultry litter underscores the public health significance of this serovar in Argentine poultry production systems. In summary, XLD agar is superior to SS agar for colony differentiation, and MSRV halo diameters of 33 mm or greater are strongly associated with successful Salmonella spp. isolation from poultry litter swab samples.EEA Concepción del UruguayFil: Hoffmann, Teresa Magali. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hoffmann, Teresa Magali. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Alcain, Andrea. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Instituto Nacional de Enfermedades Infecciosas. Servicios de Enterobacterias; ArgentinaFil: Moroni, Mirian. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Instituto Nacional de Enfermedades Infecciosas. Servicios de Enterobacterias; ArgentinaFil: Viñas, María Rosa. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Instituto Nacional de Enfermedades Infecciosas. Servicios de Enterobacterias; ArgentinaFil: Tellez-Isaias, Guillermo. Gut Health LLC; Estados UnidosFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología. Sede Basavilbaso; ArgentinaGMPC2026-06-22T12:53:08Z2026-06-22T12:53:08Z2026-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26730https://gmpc-akademie.de/articles/gjvr/single/3562703-1322https://doi.org/10.51585/gjvr.2026.2.0193German Journal of Veterinary Research 6 (2) : 124-136. (2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina .......... (nation) (World, South America)7006477info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:22Zoai:localhost:20.500.12123/26730instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:23.664INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in Argentina
title Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in Argentina
spellingShingle Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in Argentina
Hoffmann, Teresa Magali
Aves de Corral
Enfermedades de los Animales
Cama (animal)
Argentina
Poultry
Salmonella
Animal Diseases
Litter for Animals
title_short Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in Argentina
title_full Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in Argentina
title_fullStr Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in Argentina
title_full_unstemmed Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in Argentina
title_sort Prevalence of Salmonella spp. and evaluation of isolation methods from naturally contaminated poultry litter swab samples in Argentina
dc.creator.none.fl_str_mv Hoffmann, Teresa Magali
Soria, Mario
Alcain, Andrea
Moroni, Mirian
Viñas, María Rosa
Tellez-Isaias, Guillermo
Bueno, Dante Javier
author Hoffmann, Teresa Magali
author_facet Hoffmann, Teresa Magali
Soria, Mario
Alcain, Andrea
Moroni, Mirian
Viñas, María Rosa
Tellez-Isaias, Guillermo
Bueno, Dante Javier
author_role author
author2 Soria, Mario
Alcain, Andrea
Moroni, Mirian
Viñas, María Rosa
Tellez-Isaias, Guillermo
Bueno, Dante Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Aves de Corral
Enfermedades de los Animales
Cama (animal)
Argentina
Poultry
Salmonella
Animal Diseases
Litter for Animals
topic Aves de Corral
Enfermedades de los Animales
Cama (animal)
Argentina
Poultry
Salmonella
Animal Diseases
Litter for Animals
dc.description.none.fl_txt_mv National poultry improvement programs utilize multistep isolation procedures for Salmonella spp. detection in poultry litter, including selective enrichment and plating on selective-differential solid media. This study assessed the prevalence and serovar distribution of Salmonella spp. in 1,004 naturally contaminated poultry litter swab samples collected across Argentina. Samples were obtained from 775 poultry farms, comprising broiler (n = 801), laying hen (n = 2), and broiler-breeder (n = 201) houses. The probability of Salmonella isolation was evaluated based on the diameter of migration zones on modified semisolid Rappaport-Vassiliadis (MSRV) agar. The selective and differential capacities of two selective-differential plating media, xylose lysine deoxycholate (XLD) agar and Salmonella-Shigella (SS) agar, were compared. The apparent prevalence of Salmonella spp. was 20.6%. The most frequently identified serovars included Salmonella ser. Enteritidis (42%), S. ser. Westhampton (13%), and S. ser. Newport (6%). An MSRV halo diameter of 33 mm was determined to be the optimal cut-off value for a high probability of Salmonella spp. isolation. No significant differences were observed between XLD and SS agars regarding selective capacity; however, XLD agar exhibited superior differential performance. The detection of S. ser. Enteritidis in poultry litter underscores the public health significance of this serovar in Argentine poultry production systems. In summary, XLD agar is superior to SS agar for colony differentiation, and MSRV halo diameters of 33 mm or greater are strongly associated with successful Salmonella spp. isolation from poultry litter swab samples.
EEA Concepción del Uruguay
Fil: Hoffmann, Teresa Magali. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hoffmann, Teresa Magali. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Alcain, Andrea. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Instituto Nacional de Enfermedades Infecciosas. Servicios de Enterobacterias; Argentina
Fil: Moroni, Mirian. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Instituto Nacional de Enfermedades Infecciosas. Servicios de Enterobacterias; Argentina
Fil: Viñas, María Rosa. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Instituto Nacional de Enfermedades Infecciosas. Servicios de Enterobacterias; Argentina
Fil: Tellez-Isaias, Guillermo. Gut Health LLC; Estados Unidos
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología. Sede Basavilbaso; Argentina
description National poultry improvement programs utilize multistep isolation procedures for Salmonella spp. detection in poultry litter, including selective enrichment and plating on selective-differential solid media. This study assessed the prevalence and serovar distribution of Salmonella spp. in 1,004 naturally contaminated poultry litter swab samples collected across Argentina. Samples were obtained from 775 poultry farms, comprising broiler (n = 801), laying hen (n = 2), and broiler-breeder (n = 201) houses. The probability of Salmonella isolation was evaluated based on the diameter of migration zones on modified semisolid Rappaport-Vassiliadis (MSRV) agar. The selective and differential capacities of two selective-differential plating media, xylose lysine deoxycholate (XLD) agar and Salmonella-Shigella (SS) agar, were compared. The apparent prevalence of Salmonella spp. was 20.6%. The most frequently identified serovars included Salmonella ser. Enteritidis (42%), S. ser. Westhampton (13%), and S. ser. Newport (6%). An MSRV halo diameter of 33 mm was determined to be the optimal cut-off value for a high probability of Salmonella spp. isolation. No significant differences were observed between XLD and SS agars regarding selective capacity; however, XLD agar exhibited superior differential performance. The detection of S. ser. Enteritidis in poultry litter underscores the public health significance of this serovar in Argentine poultry production systems. In summary, XLD agar is superior to SS agar for colony differentiation, and MSRV halo diameters of 33 mm or greater are strongly associated with successful Salmonella spp. isolation from poultry litter swab samples.
publishDate 2026
dc.date.none.fl_str_mv 2026-06-22T12:53:08Z
2026-06-22T12:53:08Z
2026-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26730
https://gmpc-akademie.de/articles/gjvr/single/356
2703-1322
https://doi.org/10.51585/gjvr.2026.2.0193
url http://hdl.handle.net/20.500.12123/26730
https://gmpc-akademie.de/articles/gjvr/single/356
https://doi.org/10.51585/gjvr.2026.2.0193
identifier_str_mv 2703-1322
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina .......... (nation) (World, South America)
7006477
dc.publisher.none.fl_str_mv GMPC
publisher.none.fl_str_mv GMPC
dc.source.none.fl_str_mv German Journal of Veterinary Research 6 (2) : 124-136. (2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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