In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes

Autores
Pappalardo, Juan Sebastián; Langellotti, Cecilia Ana; Di Giacomo, Sebastián; Olivera, Valeria Soledad; Quattrocchi, Valeria; Zamorano, Patricia Ines; Hartner, William C.; Levchenko, Tatyana S.; Torchilin, Vladimir P.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATpliposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipidterminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6.
Instituto de Virología
Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Pappalardo, Juan Sebastián. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Hartner, William C. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Levchenko, Tatyana S. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fuente
International Journal of Nanomedicine 9 (1) : 963-973. (2014)
Materia
Transfección
In vitro
Trastorno de la Médula Ósea
Estructura Celular
Herpes Virus Bovino
Interleuquina
Transfection
Bone Marrow Disorders
Cell Structure
Bovine Herpesvirus
Interleukins
TAT Peptide
Dendritic Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27667
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27667
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomesPappalardo, Juan SebastiánLangellotti, Cecilia AnaDi Giacomo, SebastiánOlivera, Valeria SoledadQuattrocchi, ValeriaZamorano, Patricia InesHartner, William C.Levchenko, Tatyana S.Torchilin, Vladimir P.TransfecciónIn vitroTrastorno de la Médula ÓseaEstructura CelularHerpes Virus BovinoInterleuquinaTransfectionBone Marrow DisordersCell StructureBovine HerpesvirusInterleukinsTAT PeptideDendritic CellsDendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATpliposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipidterminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6.Instituto de VirologíaFil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Pappalardo, Juan Sebastián. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados UnidosFil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Hartner, William C. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados UnidosFil: Levchenko, Tatyana S. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados UnidosFil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados UnidosTaylor & Francis2026-09-04T18:11:52Z2026-09-04T18:11:52Z2014-02-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/276671176-9114 (impreso)1178-2013 (online)https://doi.org/10.2147/IJN.S53432International Journal of Nanomedicine 9 (1) : 963-973. (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:47Zoai:localhost:20.500.12123/27667instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:48.243INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
spellingShingle In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
Pappalardo, Juan Sebastián
Transfección
In vitro
Trastorno de la Médula Ósea
Estructura Celular
Herpes Virus Bovino
Interleuquina
Transfection
Bone Marrow Disorders
Cell Structure
Bovine Herpesvirus
Interleukins
TAT Peptide
Dendritic Cells
title_short In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_full In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_fullStr In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_full_unstemmed In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_sort In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
dc.creator.none.fl_str_mv Pappalardo, Juan Sebastián
Langellotti, Cecilia Ana
Di Giacomo, Sebastián
Olivera, Valeria Soledad
Quattrocchi, Valeria
Zamorano, Patricia Ines
Hartner, William C.
Levchenko, Tatyana S.
Torchilin, Vladimir P.
author Pappalardo, Juan Sebastián
author_facet Pappalardo, Juan Sebastián
Langellotti, Cecilia Ana
Di Giacomo, Sebastián
Olivera, Valeria Soledad
Quattrocchi, Valeria
Zamorano, Patricia Ines
Hartner, William C.
Levchenko, Tatyana S.
Torchilin, Vladimir P.
author_role author
author2 Langellotti, Cecilia Ana
Di Giacomo, Sebastián
Olivera, Valeria Soledad
Quattrocchi, Valeria
Zamorano, Patricia Ines
Hartner, William C.
Levchenko, Tatyana S.
Torchilin, Vladimir P.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Transfección
In vitro
Trastorno de la Médula Ósea
Estructura Celular
Herpes Virus Bovino
Interleuquina
Transfection
Bone Marrow Disorders
Cell Structure
Bovine Herpesvirus
Interleukins
TAT Peptide
Dendritic Cells
topic Transfección
In vitro
Trastorno de la Médula Ósea
Estructura Celular
Herpes Virus Bovino
Interleuquina
Transfection
Bone Marrow Disorders
Cell Structure
Bovine Herpesvirus
Interleukins
TAT Peptide
Dendritic Cells
dc.description.none.fl_txt_mv Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATpliposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipidterminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6.
Instituto de Virología
Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Pappalardo, Juan Sebastián. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Hartner, William C. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Levchenko, Tatyana S. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
description Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATpliposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipidterminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-13
2026-09-04T18:11:52Z
2026-09-04T18:11:52Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27667
1176-9114 (impreso)
1178-2013 (online)
https://doi.org/10.2147/IJN.S53432
url http://hdl.handle.net/20.500.12123/27667
https://doi.org/10.2147/IJN.S53432
identifier_str_mv 1176-9114 (impreso)
1178-2013 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv International Journal of Nanomedicine 9 (1) : 963-973. (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.754232

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