Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)

Autores
Antonuccio, Gisele Ivonne; Candas, Lucas; Sauka, Diego Hernan
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacillus thuringiensis is the most extensively studied entomopathogenic bacterium worldwide; however, its sublethal effects on beetles remain poorly characterized. The aim of this study was to evaluate the toxicity of a previously selected Argentine strain of B. thuringiensis on second-instar Alphitobius diaperinus larvae during an initial 14 days of exposure, and to assess its effects at day 14 and throughout the remainder of the life cycle until death. Three treatments were applied: control, LC30, and LC50. Larval, pupal, and adult weight and body surface area were recorded, and nutritional composition was quantified using colorimetric methods. Insect status was monitored every 48–72 h over a total period of 540 days, until the death of the last individual. Among the evaluated variables, statistically significant differences between control and treatment groups were detected in larval area and weight, in the survival analysis and in two nutritional components: total protein and lipid content per larva. Overall, the results demonstrate that initial sublethal exposure to B. thuringiensis induces chronic lethal effects with delayed mortality in A. diaperinus, indicating irreversible physiological damage. This provides valuable information not only for understanding the biology of this insect but also for stakeholders involved in the productive scaling of beetle-targeted bioinputs.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Candás, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Insects 17 (2) : 213 (February 2026)
Materia
Biological Control
Viruses
Control Biológico
Virus
Bacillus thuringiensis
Alphitobius diaperinus
Biocontrol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27221
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27221
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network_name_str INTA Digital (INTA)
spelling Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)Antonuccio, Gisele IvonneCandas, LucasSauka, Diego HernanBiological ControlVirusesControl BiológicoVirusBacillus thuringiensisAlphitobius diaperinusBiocontrolBacillus thuringiensis is the most extensively studied entomopathogenic bacterium worldwide; however, its sublethal effects on beetles remain poorly characterized. The aim of this study was to evaluate the toxicity of a previously selected Argentine strain of B. thuringiensis on second-instar Alphitobius diaperinus larvae during an initial 14 days of exposure, and to assess its effects at day 14 and throughout the remainder of the life cycle until death. Three treatments were applied: control, LC30, and LC50. Larval, pupal, and adult weight and body surface area were recorded, and nutritional composition was quantified using colorimetric methods. Insect status was monitored every 48–72 h over a total period of 540 days, until the death of the last individual. Among the evaluated variables, statistically significant differences between control and treatment groups were detected in larval area and weight, in the survival analysis and in two nutritional components: total protein and lipid content per larva. Overall, the results demonstrate that initial sublethal exposure to B. thuringiensis induces chronic lethal effects with delayed mortality in A. diaperinus, indicating irreversible physiological damage. This provides valuable information not only for understanding the biology of this insect but also for stakeholders involved in the productive scaling of beetle-targeted bioinputs.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Candás, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2026-07-31T11:03:45Z2026-07-31T11:03:45Z2026-02-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27221https://www.mdpi.com/2075-4450/17/2/2132075-4450https://doi.org/10.3390/insects17020213Insects 17 (2) : 213 (February 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Saludinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:36Zoai:localhost:20.500.12123/27221instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:37.487INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)
spellingShingle Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)
Antonuccio, Gisele Ivonne
Biological Control
Viruses
Control Biológico
Virus
Bacillus thuringiensis
Alphitobius diaperinus
Biocontrol
title_short Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_full Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_fullStr Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_full_unstemmed Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_sort Initial Sublethal Exposure to an Argentine Bacillus thuringiensis Strain Induces Chronic Toxicity and Delayed Mortality in Alphitobius diaperinus (Coleoptera: Tenebrionidae)
dc.creator.none.fl_str_mv Antonuccio, Gisele Ivonne
Candas, Lucas
Sauka, Diego Hernan
author Antonuccio, Gisele Ivonne
author_facet Antonuccio, Gisele Ivonne
Candas, Lucas
Sauka, Diego Hernan
author_role author
author2 Candas, Lucas
Sauka, Diego Hernan
author2_role author
author
dc.subject.none.fl_str_mv Biological Control
Viruses
Control Biológico
Virus
Bacillus thuringiensis
Alphitobius diaperinus
Biocontrol
topic Biological Control
Viruses
Control Biológico
Virus
Bacillus thuringiensis
Alphitobius diaperinus
Biocontrol
dc.description.none.fl_txt_mv Bacillus thuringiensis is the most extensively studied entomopathogenic bacterium worldwide; however, its sublethal effects on beetles remain poorly characterized. The aim of this study was to evaluate the toxicity of a previously selected Argentine strain of B. thuringiensis on second-instar Alphitobius diaperinus larvae during an initial 14 days of exposure, and to assess its effects at day 14 and throughout the remainder of the life cycle until death. Three treatments were applied: control, LC30, and LC50. Larval, pupal, and adult weight and body surface area were recorded, and nutritional composition was quantified using colorimetric methods. Insect status was monitored every 48–72 h over a total period of 540 days, until the death of the last individual. Among the evaluated variables, statistically significant differences between control and treatment groups were detected in larval area and weight, in the survival analysis and in two nutritional components: total protein and lipid content per larva. Overall, the results demonstrate that initial sublethal exposure to B. thuringiensis induces chronic lethal effects with delayed mortality in A. diaperinus, indicating irreversible physiological damage. This provides valuable information not only for understanding the biology of this insect but also for stakeholders involved in the productive scaling of beetle-targeted bioinputs.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Candás, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Bacillus thuringiensis is the most extensively studied entomopathogenic bacterium worldwide; however, its sublethal effects on beetles remain poorly characterized. The aim of this study was to evaluate the toxicity of a previously selected Argentine strain of B. thuringiensis on second-instar Alphitobius diaperinus larvae during an initial 14 days of exposure, and to assess its effects at day 14 and throughout the remainder of the life cycle until death. Three treatments were applied: control, LC30, and LC50. Larval, pupal, and adult weight and body surface area were recorded, and nutritional composition was quantified using colorimetric methods. Insect status was monitored every 48–72 h over a total period of 540 days, until the death of the last individual. Among the evaluated variables, statistically significant differences between control and treatment groups were detected in larval area and weight, in the survival analysis and in two nutritional components: total protein and lipid content per larva. Overall, the results demonstrate that initial sublethal exposure to B. thuringiensis induces chronic lethal effects with delayed mortality in A. diaperinus, indicating irreversible physiological damage. This provides valuable information not only for understanding the biology of this insect but also for stakeholders involved in the productive scaling of beetle-targeted bioinputs.
publishDate 2026
dc.date.none.fl_str_mv 2026-07-31T11:03:45Z
2026-07-31T11:03:45Z
2026-02-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27221
https://www.mdpi.com/2075-4450/17/2/213
2075-4450
https://doi.org/10.3390/insects17020213
url http://hdl.handle.net/20.500.12123/27221
https://www.mdpi.com/2075-4450/17/2/213
https://doi.org/10.3390/insects17020213
identifier_str_mv 2075-4450
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Insects 17 (2) : 213 (February 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.733355

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