Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions

Autores
Loeblein, Jaqueline Suelen; Alves, Luis Francisco Angeli; Araujo Coracini, Miryan Denise; Schapovaloff, Maria Elena
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gyropsylla spegazziniana is one of the most important pest of yerba mate by sap-sucking that lead to leaves deformation and there are no insecticides registered for its control. The present study describes a new technique to be used in bioassays with the insect. Galls were collected from infested yerba mate plants. In the laboratory, the galls were partially opened and distributed in plastic containers. The emerged adults were transferred to plastic containers with the perforated bottom and a central opening in the lid, and subjected to spraying. After that, insects were transferred to yerba mate seedlings in plastic cages and maintained in laboratory to daily assessment. The use of a lid with central opening prevented the insects escaping, and avoiding the previous anesthetic to spraying. The mortality was less than 10% after 10 days. The technique was considered adequate for maintenance and performance of laboratory bioassays.
EEA Montecarlo
Fil: Loeblein, Jaqueline S. Universidade Estadual do Oeste do Paraná-UNIOESTE; Brasil
Fil: Alves, Luis F. A. Universidade Estadual do Oeste do Paraná-UNIOESTE; Brasil
Fil: Araujo Coracini, Miryan D. Universidade Estadual do Oeste do Paraná-UNIOESTE; Brasil
Fil: Schapovaloff, María E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina
Fuente
Floresta e Ambiente 26 (3) : e20180419. (2019)
Materia
Ensayos Biológicos
Ilex paraguariensis
Plagas de Plantas
Técnicas de Laboratorio
Insectos
Bio-assays
Plant Pests
Laboratory Methods
Insects
Ampolla de la Yerba Mate
Paraguayan Tea Ampule
Gyropsylla spegazziniana
Yerba Mate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27535
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oai_identifier_str oai:localhost:20.500.12123/27535
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network_name_str INTA Digital (INTA)
spelling Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro ConditionsLoeblein, Jaqueline SuelenAlves, Luis Francisco AngeliAraujo Coracini, Miryan DeniseSchapovaloff, Maria ElenaEnsayos BiológicosIlex paraguariensisPlagas de PlantasTécnicas de LaboratorioInsectosBio-assaysPlant PestsLaboratory MethodsInsectsAmpolla de la Yerba MateParaguayan Tea AmpuleGyropsylla spegazzinianaYerba MateGyropsylla spegazziniana is one of the most important pest of yerba mate by sap-sucking that lead to leaves deformation and there are no insecticides registered for its control. The present study describes a new technique to be used in bioassays with the insect. Galls were collected from infested yerba mate plants. In the laboratory, the galls were partially opened and distributed in plastic containers. The emerged adults were transferred to plastic containers with the perforated bottom and a central opening in the lid, and subjected to spraying. After that, insects were transferred to yerba mate seedlings in plastic cages and maintained in laboratory to daily assessment. The use of a lid with central opening prevented the insects escaping, and avoiding the previous anesthetic to spraying. The mortality was less than 10% after 10 days. The technique was considered adequate for maintenance and performance of laboratory bioassays.EEA MontecarloFil: Loeblein, Jaqueline S. Universidade Estadual do Oeste do Paraná-UNIOESTE; BrasilFil: Alves, Luis F. A. Universidade Estadual do Oeste do Paraná-UNIOESTE; BrasilFil: Araujo Coracini, Miryan D. Universidade Estadual do Oeste do Paraná-UNIOESTE; BrasilFil: Schapovaloff, María E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; ArgentinaInstituto de Florestas, Universidade Federal Rural do Rio de Janeiro2026-08-25T14:12:10Z2026-08-25T14:12:10Z2019-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27535https://www.floram.org/journal/floram/article/doi/10.1590/2179-8087.0419182179-8087http://dx.doi.org/10.1590/2179-8087.041918Floresta e Ambiente 26 (3) : e20180419. (2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:43Zoai:localhost:20.500.12123/27535instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:44.937INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
title Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
spellingShingle Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
Loeblein, Jaqueline Suelen
Ensayos Biológicos
Ilex paraguariensis
Plagas de Plantas
Técnicas de Laboratorio
Insectos
Bio-assays
Plant Pests
Laboratory Methods
Insects
Ampolla de la Yerba Mate
Paraguayan Tea Ampule
Gyropsylla spegazziniana
Yerba Mate
title_short Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
title_full Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
title_fullStr Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
title_full_unstemmed Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
title_sort Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
dc.creator.none.fl_str_mv Loeblein, Jaqueline Suelen
Alves, Luis Francisco Angeli
Araujo Coracini, Miryan Denise
Schapovaloff, Maria Elena
author Loeblein, Jaqueline Suelen
author_facet Loeblein, Jaqueline Suelen
Alves, Luis Francisco Angeli
Araujo Coracini, Miryan Denise
Schapovaloff, Maria Elena
author_role author
author2 Alves, Luis Francisco Angeli
Araujo Coracini, Miryan Denise
Schapovaloff, Maria Elena
author2_role author
author
author
dc.subject.none.fl_str_mv Ensayos Biológicos
Ilex paraguariensis
Plagas de Plantas
Técnicas de Laboratorio
Insectos
Bio-assays
Plant Pests
Laboratory Methods
Insects
Ampolla de la Yerba Mate
Paraguayan Tea Ampule
Gyropsylla spegazziniana
Yerba Mate
topic Ensayos Biológicos
Ilex paraguariensis
Plagas de Plantas
Técnicas de Laboratorio
Insectos
Bio-assays
Plant Pests
Laboratory Methods
Insects
Ampolla de la Yerba Mate
Paraguayan Tea Ampule
Gyropsylla spegazziniana
Yerba Mate
dc.description.none.fl_txt_mv Gyropsylla spegazziniana is one of the most important pest of yerba mate by sap-sucking that lead to leaves deformation and there are no insecticides registered for its control. The present study describes a new technique to be used in bioassays with the insect. Galls were collected from infested yerba mate plants. In the laboratory, the galls were partially opened and distributed in plastic containers. The emerged adults were transferred to plastic containers with the perforated bottom and a central opening in the lid, and subjected to spraying. After that, insects were transferred to yerba mate seedlings in plastic cages and maintained in laboratory to daily assessment. The use of a lid with central opening prevented the insects escaping, and avoiding the previous anesthetic to spraying. The mortality was less than 10% after 10 days. The technique was considered adequate for maintenance and performance of laboratory bioassays.
EEA Montecarlo
Fil: Loeblein, Jaqueline S. Universidade Estadual do Oeste do Paraná-UNIOESTE; Brasil
Fil: Alves, Luis F. A. Universidade Estadual do Oeste do Paraná-UNIOESTE; Brasil
Fil: Araujo Coracini, Miryan D. Universidade Estadual do Oeste do Paraná-UNIOESTE; Brasil
Fil: Schapovaloff, María E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina
description Gyropsylla spegazziniana is one of the most important pest of yerba mate by sap-sucking that lead to leaves deformation and there are no insecticides registered for its control. The present study describes a new technique to be used in bioassays with the insect. Galls were collected from infested yerba mate plants. In the laboratory, the galls were partially opened and distributed in plastic containers. The emerged adults were transferred to plastic containers with the perforated bottom and a central opening in the lid, and subjected to spraying. After that, insects were transferred to yerba mate seedlings in plastic cages and maintained in laboratory to daily assessment. The use of a lid with central opening prevented the insects escaping, and avoiding the previous anesthetic to spraying. The mortality was less than 10% after 10 days. The technique was considered adequate for maintenance and performance of laboratory bioassays.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-01
2026-08-25T14:12:10Z
2026-08-25T14:12:10Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27535
https://www.floram.org/journal/floram/article/doi/10.1590/2179-8087.041918
2179-8087
http://dx.doi.org/10.1590/2179-8087.041918
url http://hdl.handle.net/20.500.12123/27535
https://www.floram.org/journal/floram/article/doi/10.1590/2179-8087.041918
http://dx.doi.org/10.1590/2179-8087.041918
identifier_str_mv 2179-8087
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto de Florestas, Universidade Federal Rural do Rio de Janeiro
publisher.none.fl_str_mv Instituto de Florestas, Universidade Federal Rural do Rio de Janeiro
dc.source.none.fl_str_mv Floresta e Ambiente 26 (3) : e20180419. (2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.754232

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