Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from Argentina

Autores
Muñiz Padilla, Esteban; Ulrich, Maria Noelia; Vila-Aiub, Martín Miguel; Sequin, Christian J.; Yanniccari, Marcos; Tuesca, Daniel Horacio; Tosto, Daniela Sandra
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: Glyphosate resistance in Lolium perenne ssp. multiflorum (Italian ryegrass), a major weed in temperate cropping systems, threatens effective weed control and crop productivity. This study characterized the glyphosate response and target-site glyphosate resistance mechanisms in three biotypes (PA, MG and HE) from northwest Pampas region. RESULTS: The resistant biotypes exhibited significantly higher glyphosate median lethal dose (LD50) and dose to reduce growth rate by 50% (GR50) values, with resistance indices ranging from 5.5 to 9.3. Both resistant biotypes (MG and HE) displayed hormesis, showing growth stimulation at sublethal glyphosate doses. Shikimic acid accumulation assays confirmed reduced glyphosate sensitivity in resistant biotypes, consistent with lower levels of EPSPS inhibition. Partial EPSPS gene sequencing revealed distinct Pro-106 substitutions: Pro-106-Ser in HE, and Pro-106-Thr in PA and MG, both previously associated with reduced glyphosate binding while maintaining enzyme activity. These results suggested at least two independent evolutionary origins of this resistance. Quantitative PCR revealed significantly increased EPSPS transcript levels in resistant biotypes without evidence of gene copy number amplification, indicating that elevated EPSPS expression may contribute to resistance in combination with target-site mutations. CONCLUSION: Glyphosate resistance in Italian ryegrass is primarily associated with target-site EPSPS mutations (Pro-106-Ser and Pro-106-Thr), whereas increased EPSPS expression may further contribute to the resistant phenotype. Hormesis in some resistant biotypes adds complexity to the resistance dynamics. Together, these findings highlight the adaptive potential of this species under recurrent glyphosate selection and underscore the need for integrated weed management strategies to delay the evolution and spread of herbicide resistance.
Instituto de Biotecnología
Fil: Muñiz Padilla, Esteban. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina
Fil: Muñiz Padilla, Esteban. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; Argentina
Fil: Ulrich, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ulrich, Maria Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vila Aiub, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: Vila Aiub, Martin Miguel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: Sequin, Christian. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina
Fil: Sequin, Christian. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; Argentina
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Tuesca, Daniel Horacio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Fil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Tosto, Daniela Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Pest Management Science : 1-11 (First published: 31 August 2026)
Materia
Lolium perenne
Lolium multiflorum
Biotypes
Glyphosate
Herbicide Resistance
Mutation
Weeds
Biotipos
Glifosato
Resistencia a los Herbicidas
Mutación
Malezas
Argentina
Raigrás
Control de Malezas
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27684
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27684
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from ArgentinaMuñiz Padilla, EstebanUlrich, Maria NoeliaVila-Aiub, Martín MiguelSequin, Christian J.Yanniccari, MarcosTuesca, Daniel HoracioTosto, Daniela SandraLolium perenneLolium multiflorumBiotypesGlyphosateHerbicide ResistanceMutationWeedsBiotiposGlifosatoResistencia a los HerbicidasMutaciónMalezasArgentinaRaigrásControl de MalezasBACKGROUND: Glyphosate resistance in Lolium perenne ssp. multiflorum (Italian ryegrass), a major weed in temperate cropping systems, threatens effective weed control and crop productivity. This study characterized the glyphosate response and target-site glyphosate resistance mechanisms in three biotypes (PA, MG and HE) from northwest Pampas region. RESULTS: The resistant biotypes exhibited significantly higher glyphosate median lethal dose (LD50) and dose to reduce growth rate by 50% (GR50) values, with resistance indices ranging from 5.5 to 9.3. Both resistant biotypes (MG and HE) displayed hormesis, showing growth stimulation at sublethal glyphosate doses. Shikimic acid accumulation assays confirmed reduced glyphosate sensitivity in resistant biotypes, consistent with lower levels of EPSPS inhibition. Partial EPSPS gene sequencing revealed distinct Pro-106 substitutions: Pro-106-Ser in HE, and Pro-106-Thr in PA and MG, both previously associated with reduced glyphosate binding while maintaining enzyme activity. These results suggested at least two independent evolutionary origins of this resistance. Quantitative PCR revealed significantly increased EPSPS transcript levels in resistant biotypes without evidence of gene copy number amplification, indicating that elevated EPSPS expression may contribute to resistance in combination with target-site mutations. CONCLUSION: Glyphosate resistance in Italian ryegrass is primarily associated with target-site EPSPS mutations (Pro-106-Ser and Pro-106-Thr), whereas increased EPSPS expression may further contribute to the resistant phenotype. Hormesis in some resistant biotypes adds complexity to the resistance dynamics. Together, these findings highlight the adaptive potential of this species under recurrent glyphosate selection and underscore the need for integrated weed management strategies to delay the evolution and spread of herbicide resistance.Instituto de BiotecnologíaFil: Muñiz Padilla, Esteban. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Muñiz Padilla, Esteban. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; ArgentinaFil: Ulrich, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ulrich, Maria Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vila Aiub, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.Fil: Vila Aiub, Martin Miguel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.Fil: Sequin, Christian. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Sequin, Christian. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; ArgentinaFil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaFil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Tuesca, Daniel Horacio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaFil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Tosto, Daniela Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2026-09-08T10:58:50Z2026-09-08T10:58:50Z2026-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27684https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.712591526-498X1526-4998https://doi.org/10.1002/ps.71259Pest Management Science : 1-11 (First published: 31 August 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina .......... (nation) (World, South America)7006477info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:47Zoai:localhost:20.500.12123/27684instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:48.501INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from Argentina
title Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from Argentina
spellingShingle Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from Argentina
Muñiz Padilla, Esteban
Lolium perenne
Lolium multiflorum
Biotypes
Glyphosate
Herbicide Resistance
Mutation
Weeds
Biotipos
Glifosato
Resistencia a los Herbicidas
Mutación
Malezas
Argentina
Raigrás
Control de Malezas
title_short Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from Argentina
title_full Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from Argentina
title_fullStr Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from Argentina
title_full_unstemmed Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from Argentina
title_sort Naturally occurring target-site EPSPS mutations and moderate EPSPS over-expression confer glyphosate resistance in Lolium perenne ssp. multiflorum biotypes from Argentina
dc.creator.none.fl_str_mv Muñiz Padilla, Esteban
Ulrich, Maria Noelia
Vila-Aiub, Martín Miguel
Sequin, Christian J.
Yanniccari, Marcos
Tuesca, Daniel Horacio
Tosto, Daniela Sandra
author Muñiz Padilla, Esteban
author_facet Muñiz Padilla, Esteban
Ulrich, Maria Noelia
Vila-Aiub, Martín Miguel
Sequin, Christian J.
Yanniccari, Marcos
Tuesca, Daniel Horacio
Tosto, Daniela Sandra
author_role author
author2 Ulrich, Maria Noelia
Vila-Aiub, Martín Miguel
Sequin, Christian J.
Yanniccari, Marcos
Tuesca, Daniel Horacio
Tosto, Daniela Sandra
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Lolium perenne
Lolium multiflorum
Biotypes
Glyphosate
Herbicide Resistance
Mutation
Weeds
Biotipos
Glifosato
Resistencia a los Herbicidas
Mutación
Malezas
Argentina
Raigrás
Control de Malezas
topic Lolium perenne
Lolium multiflorum
Biotypes
Glyphosate
Herbicide Resistance
Mutation
Weeds
Biotipos
Glifosato
Resistencia a los Herbicidas
Mutación
Malezas
Argentina
Raigrás
Control de Malezas
dc.description.none.fl_txt_mv BACKGROUND: Glyphosate resistance in Lolium perenne ssp. multiflorum (Italian ryegrass), a major weed in temperate cropping systems, threatens effective weed control and crop productivity. This study characterized the glyphosate response and target-site glyphosate resistance mechanisms in three biotypes (PA, MG and HE) from northwest Pampas region. RESULTS: The resistant biotypes exhibited significantly higher glyphosate median lethal dose (LD50) and dose to reduce growth rate by 50% (GR50) values, with resistance indices ranging from 5.5 to 9.3. Both resistant biotypes (MG and HE) displayed hormesis, showing growth stimulation at sublethal glyphosate doses. Shikimic acid accumulation assays confirmed reduced glyphosate sensitivity in resistant biotypes, consistent with lower levels of EPSPS inhibition. Partial EPSPS gene sequencing revealed distinct Pro-106 substitutions: Pro-106-Ser in HE, and Pro-106-Thr in PA and MG, both previously associated with reduced glyphosate binding while maintaining enzyme activity. These results suggested at least two independent evolutionary origins of this resistance. Quantitative PCR revealed significantly increased EPSPS transcript levels in resistant biotypes without evidence of gene copy number amplification, indicating that elevated EPSPS expression may contribute to resistance in combination with target-site mutations. CONCLUSION: Glyphosate resistance in Italian ryegrass is primarily associated with target-site EPSPS mutations (Pro-106-Ser and Pro-106-Thr), whereas increased EPSPS expression may further contribute to the resistant phenotype. Hormesis in some resistant biotypes adds complexity to the resistance dynamics. Together, these findings highlight the adaptive potential of this species under recurrent glyphosate selection and underscore the need for integrated weed management strategies to delay the evolution and spread of herbicide resistance.
Instituto de Biotecnología
Fil: Muñiz Padilla, Esteban. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina
Fil: Muñiz Padilla, Esteban. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; Argentina
Fil: Ulrich, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ulrich, Maria Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vila Aiub, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: Vila Aiub, Martin Miguel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: Sequin, Christian. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina
Fil: Sequin, Christian. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; Argentina
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Tuesca, Daniel Horacio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Fil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Tosto, Daniela Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description BACKGROUND: Glyphosate resistance in Lolium perenne ssp. multiflorum (Italian ryegrass), a major weed in temperate cropping systems, threatens effective weed control and crop productivity. This study characterized the glyphosate response and target-site glyphosate resistance mechanisms in three biotypes (PA, MG and HE) from northwest Pampas region. RESULTS: The resistant biotypes exhibited significantly higher glyphosate median lethal dose (LD50) and dose to reduce growth rate by 50% (GR50) values, with resistance indices ranging from 5.5 to 9.3. Both resistant biotypes (MG and HE) displayed hormesis, showing growth stimulation at sublethal glyphosate doses. Shikimic acid accumulation assays confirmed reduced glyphosate sensitivity in resistant biotypes, consistent with lower levels of EPSPS inhibition. Partial EPSPS gene sequencing revealed distinct Pro-106 substitutions: Pro-106-Ser in HE, and Pro-106-Thr in PA and MG, both previously associated with reduced glyphosate binding while maintaining enzyme activity. These results suggested at least two independent evolutionary origins of this resistance. Quantitative PCR revealed significantly increased EPSPS transcript levels in resistant biotypes without evidence of gene copy number amplification, indicating that elevated EPSPS expression may contribute to resistance in combination with target-site mutations. CONCLUSION: Glyphosate resistance in Italian ryegrass is primarily associated with target-site EPSPS mutations (Pro-106-Ser and Pro-106-Thr), whereas increased EPSPS expression may further contribute to the resistant phenotype. Hormesis in some resistant biotypes adds complexity to the resistance dynamics. Together, these findings highlight the adaptive potential of this species under recurrent glyphosate selection and underscore the need for integrated weed management strategies to delay the evolution and spread of herbicide resistance.
publishDate 2026
dc.date.none.fl_str_mv 2026-09-08T10:58:50Z
2026-09-08T10:58:50Z
2026-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27684
https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.71259
1526-498X
1526-4998
https://doi.org/10.1002/ps.71259
url http://hdl.handle.net/20.500.12123/27684
https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.71259
https://doi.org/10.1002/ps.71259
identifier_str_mv 1526-498X
1526-4998
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina .......... (nation) (World, South America)
7006477
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Pest Management Science : 1-11 (First published: 31 August 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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