Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilers

Autores
Buzetti, Guillermina; Saizar, Magdalena; Charriere, Maria Viviana; Fain Binda, Virginia; Iglesias, Bernardo Fabricio; Lavore, Andrés
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Choline is an essential nutrient involved in lipid transport, hepatic metabolism, and methyl-group donation in poultry. Although synthetic choline chloride is widely used in broiler nutrition, natural alternatives have emerged as potential substitutes with additional metabolic benefits. The present study evaluated the effects of synthetic and vegetal-derived choline supplementation on growth performance, biochemical parameters, and hepatic transcriptomic responses in broiler chickens. We compared three dietary treatments: T1-basal: basal diet without supplemental choline; T2-ClCh: basal diet supplemented with synthetic choline chloride; and T3-VegCh: basal diet supplemented with vegetal choline. Productive performance was evaluated throughout the 42d experimental period, while liver transcriptomic analyses were performed at 28 days using RNA sequencing. Differentially expressed genes (DEGs), KEGG pathway enrichment analysis, weighted gene co-expression network analysis (WGCNA), and integrative multivariate analyses were performed. Choline supplementation improved final body weight, average daily gain, and feed conversion ratio during the finisher B phase and over the overall experimental period. Both choline sources reduced total serum lipid concentrations, whereas VegCh additionally reduced alkaline phosphatase activity. Transcriptomic analyses revealed distinct hepatic metabolic profiles between treatments. ClCh supplementation induced a transcriptional program enriched in pro-inflammatory signaling, immune activation, and stress-response pathways, suggesting a metabolically active and inflammatory hepatic state. In contrast, VegCh supplementation enriched pathways associated with glycolysis/gluconeogenesis, the TCA cycle, fatty acid metabolism, PPAR signaling, insulin signaling, mTOR signaling, and glycerolipid metabolism, indicating an anabolic phenotype characterized by lipid biosynthesis and endocrine regulation. Overall, both choline sources improved productive performance; however, VegCh promoted a distinct hepatic transcriptional profile characterized by enrichment in metabolic pathways and reduced activation of immune-related signaling pathways compared with the synthetic source. These findings provide new insights into the molecular effects of dietary choline sources in broiler chickens and support the potential use of plant-derived choline as a functional nutritional strategy to improve metabolic fitness in poultry.
EEA Pergamino
Fil: Buzetti, Guillermina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Buzetti, Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Saizar, Magdalena. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Charriere, María Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Avicultura; Argentina
Fil: Fain Binda, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Avicultura; Argentina
Fil: Fain Binda, Virginia. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Iglesias, Bernardo Fabricio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Avicultura; Argentina
Fil: Iglesias, Bernardo Fabricio. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Lavore, Andrés. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Lavore, Andrés. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; Argentina
Fuente
Poultry Science 105 (12) : 107699. (December 2026)
Materia
Avicultura
Pollo
Pollo de Engorde
Suplementos Alimentarios
Colina
Transcriptómica
Metabolismo
Aviculture
Chickens
Broiler Chickens
Food Supplements
Choline
Transcriptomics
Metabolism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27810
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27810
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilersBuzetti, GuillerminaSaizar, MagdalenaCharriere, Maria VivianaFain Binda, VirginiaIglesias, Bernardo FabricioLavore, AndrésAviculturaPolloPollo de EngordeSuplementos AlimentariosColinaTranscriptómicaMetabolismoAvicultureChickensBroiler ChickensFood SupplementsCholineTranscriptomicsMetabolismCholine is an essential nutrient involved in lipid transport, hepatic metabolism, and methyl-group donation in poultry. Although synthetic choline chloride is widely used in broiler nutrition, natural alternatives have emerged as potential substitutes with additional metabolic benefits. The present study evaluated the effects of synthetic and vegetal-derived choline supplementation on growth performance, biochemical parameters, and hepatic transcriptomic responses in broiler chickens. We compared three dietary treatments: T1-basal: basal diet without supplemental choline; T2-ClCh: basal diet supplemented with synthetic choline chloride; and T3-VegCh: basal diet supplemented with vegetal choline. Productive performance was evaluated throughout the 42d experimental period, while liver transcriptomic analyses were performed at 28 days using RNA sequencing. Differentially expressed genes (DEGs), KEGG pathway enrichment analysis, weighted gene co-expression network analysis (WGCNA), and integrative multivariate analyses were performed. Choline supplementation improved final body weight, average daily gain, and feed conversion ratio during the finisher B phase and over the overall experimental period. Both choline sources reduced total serum lipid concentrations, whereas VegCh additionally reduced alkaline phosphatase activity. Transcriptomic analyses revealed distinct hepatic metabolic profiles between treatments. ClCh supplementation induced a transcriptional program enriched in pro-inflammatory signaling, immune activation, and stress-response pathways, suggesting a metabolically active and inflammatory hepatic state. In contrast, VegCh supplementation enriched pathways associated with glycolysis/gluconeogenesis, the TCA cycle, fatty acid metabolism, PPAR signaling, insulin signaling, mTOR signaling, and glycerolipid metabolism, indicating an anabolic phenotype characterized by lipid biosynthesis and endocrine regulation. Overall, both choline sources improved productive performance; however, VegCh promoted a distinct hepatic transcriptional profile characterized by enrichment in metabolic pathways and reduced activation of immune-related signaling pathways compared with the synthetic source. These findings provide new insights into the molecular effects of dietary choline sources in broiler chickens and support the potential use of plant-derived choline as a functional nutritional strategy to improve metabolic fitness in poultry.EEA PergaminoFil: Buzetti, Guillermina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; ArgentinaFil: Buzetti, Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Saizar, Magdalena. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; ArgentinaFil: Charriere, María Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Avicultura; ArgentinaFil: Fain Binda, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Avicultura; ArgentinaFil: Fain Binda, Virginia. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; ArgentinaFil: Iglesias, Bernardo Fabricio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Avicultura; ArgentinaFil: Iglesias, Bernardo Fabricio. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; ArgentinaFil: Lavore, Andrés. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; ArgentinaFil: Lavore, Andrés. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; ArgentinaElsevier2026-09-16T10:15:26Z2026-09-16T10:15:26Z2026-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27810https://www.sciencedirect.com/science/article/pii/S00325791260133370032-57911525-3171 (online)https://doi.org/10.1016/j.psj.2026.107699Poultry Science 105 (12) : 107699. (December 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-10-01T10:25:47Zoai:localhost:20.500.12123/27810instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-10-01 10:25:49.041INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilers
title Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilers
spellingShingle Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilers
Buzetti, Guillermina
Avicultura
Pollo
Pollo de Engorde
Suplementos Alimentarios
Colina
Transcriptómica
Metabolismo
Aviculture
Chickens
Broiler Chickens
Food Supplements
Choline
Transcriptomics
Metabolism
title_short Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilers
title_full Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilers
title_fullStr Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilers
title_full_unstemmed Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilers
title_sort Vegetal choline supplementation modulates hepatic metabolism and immune-related transcriptional responses in broilers
dc.creator.none.fl_str_mv Buzetti, Guillermina
Saizar, Magdalena
Charriere, Maria Viviana
Fain Binda, Virginia
Iglesias, Bernardo Fabricio
Lavore, Andrés
author Buzetti, Guillermina
author_facet Buzetti, Guillermina
Saizar, Magdalena
Charriere, Maria Viviana
Fain Binda, Virginia
Iglesias, Bernardo Fabricio
Lavore, Andrés
author_role author
author2 Saizar, Magdalena
Charriere, Maria Viviana
Fain Binda, Virginia
Iglesias, Bernardo Fabricio
Lavore, Andrés
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Avicultura
Pollo
Pollo de Engorde
Suplementos Alimentarios
Colina
Transcriptómica
Metabolismo
Aviculture
Chickens
Broiler Chickens
Food Supplements
Choline
Transcriptomics
Metabolism
topic Avicultura
Pollo
Pollo de Engorde
Suplementos Alimentarios
Colina
Transcriptómica
Metabolismo
Aviculture
Chickens
Broiler Chickens
Food Supplements
Choline
Transcriptomics
Metabolism
dc.description.none.fl_txt_mv Choline is an essential nutrient involved in lipid transport, hepatic metabolism, and methyl-group donation in poultry. Although synthetic choline chloride is widely used in broiler nutrition, natural alternatives have emerged as potential substitutes with additional metabolic benefits. The present study evaluated the effects of synthetic and vegetal-derived choline supplementation on growth performance, biochemical parameters, and hepatic transcriptomic responses in broiler chickens. We compared three dietary treatments: T1-basal: basal diet without supplemental choline; T2-ClCh: basal diet supplemented with synthetic choline chloride; and T3-VegCh: basal diet supplemented with vegetal choline. Productive performance was evaluated throughout the 42d experimental period, while liver transcriptomic analyses were performed at 28 days using RNA sequencing. Differentially expressed genes (DEGs), KEGG pathway enrichment analysis, weighted gene co-expression network analysis (WGCNA), and integrative multivariate analyses were performed. Choline supplementation improved final body weight, average daily gain, and feed conversion ratio during the finisher B phase and over the overall experimental period. Both choline sources reduced total serum lipid concentrations, whereas VegCh additionally reduced alkaline phosphatase activity. Transcriptomic analyses revealed distinct hepatic metabolic profiles between treatments. ClCh supplementation induced a transcriptional program enriched in pro-inflammatory signaling, immune activation, and stress-response pathways, suggesting a metabolically active and inflammatory hepatic state. In contrast, VegCh supplementation enriched pathways associated with glycolysis/gluconeogenesis, the TCA cycle, fatty acid metabolism, PPAR signaling, insulin signaling, mTOR signaling, and glycerolipid metabolism, indicating an anabolic phenotype characterized by lipid biosynthesis and endocrine regulation. Overall, both choline sources improved productive performance; however, VegCh promoted a distinct hepatic transcriptional profile characterized by enrichment in metabolic pathways and reduced activation of immune-related signaling pathways compared with the synthetic source. These findings provide new insights into the molecular effects of dietary choline sources in broiler chickens and support the potential use of plant-derived choline as a functional nutritional strategy to improve metabolic fitness in poultry.
EEA Pergamino
Fil: Buzetti, Guillermina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Buzetti, Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Saizar, Magdalena. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Charriere, María Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Avicultura; Argentina
Fil: Fain Binda, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Avicultura; Argentina
Fil: Fain Binda, Virginia. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Iglesias, Bernardo Fabricio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Avicultura; Argentina
Fil: Iglesias, Bernardo Fabricio. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Lavore, Andrés. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Centro de BioInvestigaciones; Argentina
Fil: Lavore, Andrés. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; Argentina
description Choline is an essential nutrient involved in lipid transport, hepatic metabolism, and methyl-group donation in poultry. Although synthetic choline chloride is widely used in broiler nutrition, natural alternatives have emerged as potential substitutes with additional metabolic benefits. The present study evaluated the effects of synthetic and vegetal-derived choline supplementation on growth performance, biochemical parameters, and hepatic transcriptomic responses in broiler chickens. We compared three dietary treatments: T1-basal: basal diet without supplemental choline; T2-ClCh: basal diet supplemented with synthetic choline chloride; and T3-VegCh: basal diet supplemented with vegetal choline. Productive performance was evaluated throughout the 42d experimental period, while liver transcriptomic analyses were performed at 28 days using RNA sequencing. Differentially expressed genes (DEGs), KEGG pathway enrichment analysis, weighted gene co-expression network analysis (WGCNA), and integrative multivariate analyses were performed. Choline supplementation improved final body weight, average daily gain, and feed conversion ratio during the finisher B phase and over the overall experimental period. Both choline sources reduced total serum lipid concentrations, whereas VegCh additionally reduced alkaline phosphatase activity. Transcriptomic analyses revealed distinct hepatic metabolic profiles between treatments. ClCh supplementation induced a transcriptional program enriched in pro-inflammatory signaling, immune activation, and stress-response pathways, suggesting a metabolically active and inflammatory hepatic state. In contrast, VegCh supplementation enriched pathways associated with glycolysis/gluconeogenesis, the TCA cycle, fatty acid metabolism, PPAR signaling, insulin signaling, mTOR signaling, and glycerolipid metabolism, indicating an anabolic phenotype characterized by lipid biosynthesis and endocrine regulation. Overall, both choline sources improved productive performance; however, VegCh promoted a distinct hepatic transcriptional profile characterized by enrichment in metabolic pathways and reduced activation of immune-related signaling pathways compared with the synthetic source. These findings provide new insights into the molecular effects of dietary choline sources in broiler chickens and support the potential use of plant-derived choline as a functional nutritional strategy to improve metabolic fitness in poultry.
publishDate 2026
dc.date.none.fl_str_mv 2026-09-16T10:15:26Z
2026-09-16T10:15:26Z
2026-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27810
https://www.sciencedirect.com/science/article/pii/S0032579126013337
0032-5791
1525-3171 (online)
https://doi.org/10.1016/j.psj.2026.107699
url http://hdl.handle.net/20.500.12123/27810
https://www.sciencedirect.com/science/article/pii/S0032579126013337
https://doi.org/10.1016/j.psj.2026.107699
identifier_str_mv 0032-5791
1525-3171 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Poultry Science 105 (12) : 107699. (December 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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