CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture

Autores
Távora, Fabiano Touzdjian Pinheiro Kohlrausch; Diniz, Francisco de Assis dos Santos; Rêgo-Machado, Camila de Moraes; Freitas, Natalia Chagas; Arraes, Fabricio Barbosa Monteiro; Andrade, Eduardo Chumbinho de; Furtado, Leila Lourenço; Osiro, Karen Ofuji; Sousa, Natalia Lima de; Cardoso, Thiago Bérgamo; Henning, Liliane Márcia Mertz; Molinari, Patricia Abrão de Oliveira; Feingold, Sergio Enrique; Hunter, Wayne B.; Sá, Maria Fátima Grossi de; Kobayashi, Adilson Kenji; Nepomuceno, Alexandre Lima; Santiago, Thais Ribeiro; Correa Molinari, Hugo Bruno
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe.
EEA Balcarce
Fil: Touzdjian Pinheiro Kohlrausch Távora, Fabiano. Universidad de Brasília. Departmento de Fitotopatología; Brasil
Fil: Touzdjian Pinheiro Kohlrausch Távora, Fabiano. Embrapa Agroenergy; Brasil
Fil: de Assis dos Santos Diniz, Francisco. Universidad de Brasília. Departmento de Fitotopatología; Brasil
Fil: de Moraes Rêgo-Machado, Camila. Universidad de Brasília. Departmento de Fitotopatología; Brasil
Fil: de Moraes Rêgo-Machado, Camila. Embrapa Agroenergy; Brasil
Fil: Chagas Freitas, Natalia. Embrapa Agroenergy; Brasil
Fil: Barbosa Monteiro Arraes, Fabricio. Embrapa Genetic Resources and Biotechnology; Brasil
Fil: Chumbinho de Andrade, Eduardo. Embrapa Cassava and Fruits; Brasil
Fil: Lourenço Furtado, Leila. Universidad de Brasília. Departmento de Fitotopatología; Brasil
Fil: Ofuji Osiro, Karen. Universidad de Brasília. Departmento de Fitotopatologia; Brasil
Fil: Ofuji Osiro, Karen. Embrapa Agroenergy; Brasil
Fil: Lima de Sousa, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Lima de Sousa, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Bérgamo Cardoso, Thiago. SEMPRE AgTech; Brasil
Fil: Mertz Henning, Liliane Márcia. Embrapa Soybean; Brasil
Fil: Abrão de Oliveira Molinari, Patricia. Embrapa Agroenergy; Brasil
Fil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Feingold, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Hunter. Wayne B. United States Departament of Agriculture. Horticultural Research Laboratory; Estados Unidos
Fil: Grossi de Sá, Maria Fátima. Embrapa Genetic Resources and Biotechnology; Brasil
Fil: Kobayashi, Adilson Kenji. Embrapa Agroenergy; Brasil
Fil: Lima Nepomuceno, Alexandre. Embrapa Soybean; Brasil
Fil: Ribeiro Santiago, Thais. Universidad de Brasília. Departmento de Fitotopatologia; Brasil
Fil: Correa Molinari, Hugo Bruno. SEMPRE AgTech; Brasil
Fuente
Frontiers in Bioengineering and Biotechnology 10 : 913728 (June 2022)
Materia
ARN
Interferencia por ARN
Transgénico
Fitomejoramiento
RNA
RNA Interference
Transgenics
Plant Breeding
CRISPR/Cas
ARN de Interferencia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27045
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spelling CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable AgricultureTávora, Fabiano Touzdjian Pinheiro KohlrauschDiniz, Francisco de Assis dos SantosRêgo-Machado, Camila de MoraesFreitas, Natalia ChagasArraes, Fabricio Barbosa MonteiroAndrade, Eduardo Chumbinho deFurtado, Leila LourençoOsiro, Karen OfujiSousa, Natalia Lima deCardoso, Thiago BérgamoHenning, Liliane Márcia MertzMolinari, Patricia Abrão de OliveiraFeingold, Sergio EnriqueHunter, Wayne B.Sá, Maria Fátima Grossi deKobayashi, Adilson KenjiNepomuceno, Alexandre LimaSantiago, Thais RibeiroCorrea Molinari, Hugo BrunoARNInterferencia por ARNTransgénicoFitomejoramientoRNARNA InterferenceTransgenicsPlant BreedingCRISPR/CasARN de InterferenciaClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe.EEA BalcarceFil: Touzdjian Pinheiro Kohlrausch Távora, Fabiano. Universidad de Brasília. Departmento de Fitotopatología; BrasilFil: Touzdjian Pinheiro Kohlrausch Távora, Fabiano. Embrapa Agroenergy; BrasilFil: de Assis dos Santos Diniz, Francisco. Universidad de Brasília. Departmento de Fitotopatología; BrasilFil: de Moraes Rêgo-Machado, Camila. Universidad de Brasília. Departmento de Fitotopatología; BrasilFil: de Moraes Rêgo-Machado, Camila. Embrapa Agroenergy; BrasilFil: Chagas Freitas, Natalia. Embrapa Agroenergy; BrasilFil: Barbosa Monteiro Arraes, Fabricio. Embrapa Genetic Resources and Biotechnology; BrasilFil: Chumbinho de Andrade, Eduardo. Embrapa Cassava and Fruits; BrasilFil: Lourenço Furtado, Leila. Universidad de Brasília. Departmento de Fitotopatología; BrasilFil: Ofuji Osiro, Karen. Universidad de Brasília. Departmento de Fitotopatologia; BrasilFil: Ofuji Osiro, Karen. Embrapa Agroenergy; BrasilFil: Lima de Sousa, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); ArgentinaFil: Lima de Sousa, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); ArgentinaFil: Bérgamo Cardoso, Thiago. SEMPRE AgTech; BrasilFil: Mertz Henning, Liliane Márcia. Embrapa Soybean; BrasilFil: Abrão de Oliveira Molinari, Patricia. Embrapa Agroenergy; BrasilFil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); ArgentinaFil: Feingold, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); ArgentinaFil: Hunter. Wayne B. United States Departament of Agriculture. Horticultural Research Laboratory; Estados UnidosFil: Grossi de Sá, Maria Fátima. Embrapa Genetic Resources and Biotechnology; BrasilFil: Kobayashi, Adilson Kenji. Embrapa Agroenergy; BrasilFil: Lima Nepomuceno, Alexandre. Embrapa Soybean; BrasilFil: Ribeiro Santiago, Thais. Universidad de Brasília. Departmento de Fitotopatologia; BrasilFil: Correa Molinari, Hugo Bruno. SEMPRE AgTech; BrasilFrontiers Media2026-07-17T11:56:16Z2026-07-17T11:56:16Z2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27045https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.913728/full2296-41852296-4185https://doi.org/10.3389/fbioe.2022.913728Frontiers in Bioengineering and Biotechnology 10 : 913728 (June 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:27Zoai:localhost:20.500.12123/27045instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:29.057INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture
title CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture
spellingShingle CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture
Távora, Fabiano Touzdjian Pinheiro Kohlrausch
ARN
Interferencia por ARN
Transgénico
Fitomejoramiento
RNA
RNA Interference
Transgenics
Plant Breeding
CRISPR/Cas
ARN de Interferencia
title_short CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture
title_full CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture
title_fullStr CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture
title_full_unstemmed CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture
title_sort CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture
dc.creator.none.fl_str_mv Távora, Fabiano Touzdjian Pinheiro Kohlrausch
Diniz, Francisco de Assis dos Santos
Rêgo-Machado, Camila de Moraes
Freitas, Natalia Chagas
Arraes, Fabricio Barbosa Monteiro
Andrade, Eduardo Chumbinho de
Furtado, Leila Lourenço
Osiro, Karen Ofuji
Sousa, Natalia Lima de
Cardoso, Thiago Bérgamo
Henning, Liliane Márcia Mertz
Molinari, Patricia Abrão de Oliveira
Feingold, Sergio Enrique
Hunter, Wayne B.
Sá, Maria Fátima Grossi de
Kobayashi, Adilson Kenji
Nepomuceno, Alexandre Lima
Santiago, Thais Ribeiro
Correa Molinari, Hugo Bruno
author Távora, Fabiano Touzdjian Pinheiro Kohlrausch
author_facet Távora, Fabiano Touzdjian Pinheiro Kohlrausch
Diniz, Francisco de Assis dos Santos
Rêgo-Machado, Camila de Moraes
Freitas, Natalia Chagas
Arraes, Fabricio Barbosa Monteiro
Andrade, Eduardo Chumbinho de
Furtado, Leila Lourenço
Osiro, Karen Ofuji
Sousa, Natalia Lima de
Cardoso, Thiago Bérgamo
Henning, Liliane Márcia Mertz
Molinari, Patricia Abrão de Oliveira
Feingold, Sergio Enrique
Hunter, Wayne B.
Sá, Maria Fátima Grossi de
Kobayashi, Adilson Kenji
Nepomuceno, Alexandre Lima
Santiago, Thais Ribeiro
Correa Molinari, Hugo Bruno
author_role author
author2 Diniz, Francisco de Assis dos Santos
Rêgo-Machado, Camila de Moraes
Freitas, Natalia Chagas
Arraes, Fabricio Barbosa Monteiro
Andrade, Eduardo Chumbinho de
Furtado, Leila Lourenço
Osiro, Karen Ofuji
Sousa, Natalia Lima de
Cardoso, Thiago Bérgamo
Henning, Liliane Márcia Mertz
Molinari, Patricia Abrão de Oliveira
Feingold, Sergio Enrique
Hunter, Wayne B.
Sá, Maria Fátima Grossi de
Kobayashi, Adilson Kenji
Nepomuceno, Alexandre Lima
Santiago, Thais Ribeiro
Correa Molinari, Hugo Bruno
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARN
Interferencia por ARN
Transgénico
Fitomejoramiento
RNA
RNA Interference
Transgenics
Plant Breeding
CRISPR/Cas
ARN de Interferencia
topic ARN
Interferencia por ARN
Transgénico
Fitomejoramiento
RNA
RNA Interference
Transgenics
Plant Breeding
CRISPR/Cas
ARN de Interferencia
dc.description.none.fl_txt_mv Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe.
EEA Balcarce
Fil: Touzdjian Pinheiro Kohlrausch Távora, Fabiano. Universidad de Brasília. Departmento de Fitotopatología; Brasil
Fil: Touzdjian Pinheiro Kohlrausch Távora, Fabiano. Embrapa Agroenergy; Brasil
Fil: de Assis dos Santos Diniz, Francisco. Universidad de Brasília. Departmento de Fitotopatología; Brasil
Fil: de Moraes Rêgo-Machado, Camila. Universidad de Brasília. Departmento de Fitotopatología; Brasil
Fil: de Moraes Rêgo-Machado, Camila. Embrapa Agroenergy; Brasil
Fil: Chagas Freitas, Natalia. Embrapa Agroenergy; Brasil
Fil: Barbosa Monteiro Arraes, Fabricio. Embrapa Genetic Resources and Biotechnology; Brasil
Fil: Chumbinho de Andrade, Eduardo. Embrapa Cassava and Fruits; Brasil
Fil: Lourenço Furtado, Leila. Universidad de Brasília. Departmento de Fitotopatología; Brasil
Fil: Ofuji Osiro, Karen. Universidad de Brasília. Departmento de Fitotopatologia; Brasil
Fil: Ofuji Osiro, Karen. Embrapa Agroenergy; Brasil
Fil: Lima de Sousa, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Lima de Sousa, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Bérgamo Cardoso, Thiago. SEMPRE AgTech; Brasil
Fil: Mertz Henning, Liliane Márcia. Embrapa Soybean; Brasil
Fil: Abrão de Oliveira Molinari, Patricia. Embrapa Agroenergy; Brasil
Fil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Feingold, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Hunter. Wayne B. United States Departament of Agriculture. Horticultural Research Laboratory; Estados Unidos
Fil: Grossi de Sá, Maria Fátima. Embrapa Genetic Resources and Biotechnology; Brasil
Fil: Kobayashi, Adilson Kenji. Embrapa Agroenergy; Brasil
Fil: Lima Nepomuceno, Alexandre. Embrapa Soybean; Brasil
Fil: Ribeiro Santiago, Thais. Universidad de Brasília. Departmento de Fitotopatologia; Brasil
Fil: Correa Molinari, Hugo Bruno. SEMPRE AgTech; Brasil
description Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe.
publishDate 2022
dc.date.none.fl_str_mv 2022
2026-07-17T11:56:16Z
2026-07-17T11:56:16Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27045
https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.913728/full
2296-4185
2296-4185
https://doi.org/10.3389/fbioe.2022.913728
url http://hdl.handle.net/20.500.12123/27045
https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.913728/full
https://doi.org/10.3389/fbioe.2022.913728
identifier_str_mv 2296-4185
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Bioengineering and Biotechnology 10 : 913728 (June 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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