Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine Pampas

Autores
Gortari, Maximiliano; Maguire, Vanina Giselle; Ezquiaga, Juan Pedro; Cicchino, Mariano Andres; Bailleres, Matias Andres; Escaray, Roberto Ulises; Ruíz, Oscar Adolfo; Llames, María Eugenia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soil microbial communities play a key role in carbon (C) cycling in agroecosystems; however, their long-term responses to contrasting management practices remain poorly understood in agricultural soils. In this study, we evaluated the effects of more than 20 years of no-till farming (NTF) and conventional tillage (CT) on soil physicochemical properties, bacterial and fungal community composition, and inferred functions related to C and nutrient cycling in the Argentine Pampas. We show that NTF increased total organic carbon (TOC) stocks in surface soils and promoted edaphic conditions associated with C stabilization, including higher cation exchange capacity and structural stability. Bacterial communities exhibited high functional redundancy and were primarily structured along sodium-related parameters, whereas fungal communities were more sensitive to management, with NTF favoring ligninolytic and symbiotic fungi that contribute to necromass formation and long-term carbon stabilization. In contrast, CT enriched opportunistic fungal guilds associated with disturbance and short-term nutrient turnover. Phylogenetic analyses revealed community assembly dominated by environmental filtering in both microbial domains. Overall, these results highlight the central role of fungi as mediators of soil C stabilization and suggest that conservation practices such as NTF enhance microbiome contributions to ecosystem services and climate change mitigation in intensively managed agroecosystems.
EEA Manfredi
Fil: Gortari, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Gortari, Maximiliano. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Gortari, Maximiliano. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina
Fil: Maguire, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Área de Mejoramiento Genético Vegetal; Argentina
Fil: Ezquiaga, Juan Pedro. Provincia de Buenos Aires. Ministerio de Desarrollo Agrario (MDA). Chacra Experimental Integrada Chascomús; ArgentinaI
Fil: Cicchino, Mariano Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado. Agencia de Extensión Rural Chascomús; Argentina
Fil: Bailleres, Matias Andres. Provincia de Buenos Aires. Ministerio de Desarrollo Agrario (MDA). Chacra Experimental Integrada Chascomús; Argentina
Fil: Escaray, Roberto Ulises. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Escaray, Roberto Ulises. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Ruíz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Ruíz, Oscar Adolfo. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Llames, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Ecología y Fotobiología Acuática; Argentina
Fil: Llames, María Eugenia. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fuente
Scientific Reports 16 : Article number: 21484. (2026)
Materia
Sistemas de Explotación
Cero-labranza
Microbiomas
Suelo
Carbono
Agroecosistemas
Labranza de Conservación
Farming Systems
Zero Tillage
Microbiomes
Soil
Carbon
Agroecosystems
Conservation Tillage
Región Pampeana, Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27432
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oai_identifier_str oai:localhost:20.500.12123/27432
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network_name_str INTA Digital (INTA)
spelling Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine PampasGortari, MaximilianoMaguire, Vanina GiselleEzquiaga, Juan PedroCicchino, Mariano AndresBailleres, Matias AndresEscaray, Roberto UlisesRuíz, Oscar AdolfoLlames, María EugeniaSistemas de ExplotaciónCero-labranzaMicrobiomasSueloCarbonoAgroecosistemasLabranza de ConservaciónFarming SystemsZero TillageMicrobiomesSoilCarbonAgroecosystemsConservation TillageRegión Pampeana, ArgentinaSoil microbial communities play a key role in carbon (C) cycling in agroecosystems; however, their long-term responses to contrasting management practices remain poorly understood in agricultural soils. In this study, we evaluated the effects of more than 20 years of no-till farming (NTF) and conventional tillage (CT) on soil physicochemical properties, bacterial and fungal community composition, and inferred functions related to C and nutrient cycling in the Argentine Pampas. We show that NTF increased total organic carbon (TOC) stocks in surface soils and promoted edaphic conditions associated with C stabilization, including higher cation exchange capacity and structural stability. Bacterial communities exhibited high functional redundancy and were primarily structured along sodium-related parameters, whereas fungal communities were more sensitive to management, with NTF favoring ligninolytic and symbiotic fungi that contribute to necromass formation and long-term carbon stabilization. In contrast, CT enriched opportunistic fungal guilds associated with disturbance and short-term nutrient turnover. Phylogenetic analyses revealed community assembly dominated by environmental filtering in both microbial domains. Overall, these results highlight the central role of fungi as mediators of soil C stabilization and suggest that conservation practices such as NTF enhance microbiome contributions to ecosystem services and climate change mitigation in intensively managed agroecosystems.EEA ManfrediFil: Gortari, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; ArgentinaFil: Gortari, Maximiliano. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; ArgentinaFil: Gortari, Maximiliano. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; ArgentinaFil: Maguire, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Área de Mejoramiento Genético Vegetal; ArgentinaFil: Ezquiaga, Juan Pedro. Provincia de Buenos Aires. Ministerio de Desarrollo Agrario (MDA). Chacra Experimental Integrada Chascomús; ArgentinaIFil: Cicchino, Mariano Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado. Agencia de Extensión Rural Chascomús; ArgentinaFil: Bailleres, Matias Andres. Provincia de Buenos Aires. Ministerio de Desarrollo Agrario (MDA). Chacra Experimental Integrada Chascomús; ArgentinaFil: Escaray, Roberto Ulises. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; ArgentinaFil: Escaray, Roberto Ulises. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; ArgentinaFil: Ruíz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; ArgentinaFil: Ruíz, Oscar Adolfo. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; ArgentinaFil: Llames, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Ecología y Fotobiología Acuática; ArgentinaFil: Llames, María Eugenia. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; ArgentinaSpringer Nature2026-08-18T11:45:27Z2026-08-18T11:45:27Z2026-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27432https://www.nature.com/articles/s41598-026-47621-42045-2322https://doi.org/10.1038/s41598-026-47621-4Scientific Reports 16 : Article number: 21484. (2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:41Zoai:localhost:20.500.12123/27432instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:43.122INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine Pampas
title Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine Pampas
spellingShingle Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine Pampas
Gortari, Maximiliano
Sistemas de Explotación
Cero-labranza
Microbiomas
Suelo
Carbono
Agroecosistemas
Labranza de Conservación
Farming Systems
Zero Tillage
Microbiomes
Soil
Carbon
Agroecosystems
Conservation Tillage
Región Pampeana, Argentina
title_short Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine Pampas
title_full Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine Pampas
title_fullStr Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine Pampas
title_full_unstemmed Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine Pampas
title_sort Associations between soil microbiomes and carbon stabilization under long-term no-till farming systems in the Argentine Pampas
dc.creator.none.fl_str_mv Gortari, Maximiliano
Maguire, Vanina Giselle
Ezquiaga, Juan Pedro
Cicchino, Mariano Andres
Bailleres, Matias Andres
Escaray, Roberto Ulises
Ruíz, Oscar Adolfo
Llames, María Eugenia
author Gortari, Maximiliano
author_facet Gortari, Maximiliano
Maguire, Vanina Giselle
Ezquiaga, Juan Pedro
Cicchino, Mariano Andres
Bailleres, Matias Andres
Escaray, Roberto Ulises
Ruíz, Oscar Adolfo
Llames, María Eugenia
author_role author
author2 Maguire, Vanina Giselle
Ezquiaga, Juan Pedro
Cicchino, Mariano Andres
Bailleres, Matias Andres
Escaray, Roberto Ulises
Ruíz, Oscar Adolfo
Llames, María Eugenia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Sistemas de Explotación
Cero-labranza
Microbiomas
Suelo
Carbono
Agroecosistemas
Labranza de Conservación
Farming Systems
Zero Tillage
Microbiomes
Soil
Carbon
Agroecosystems
Conservation Tillage
Región Pampeana, Argentina
topic Sistemas de Explotación
Cero-labranza
Microbiomas
Suelo
Carbono
Agroecosistemas
Labranza de Conservación
Farming Systems
Zero Tillage
Microbiomes
Soil
Carbon
Agroecosystems
Conservation Tillage
Región Pampeana, Argentina
dc.description.none.fl_txt_mv Soil microbial communities play a key role in carbon (C) cycling in agroecosystems; however, their long-term responses to contrasting management practices remain poorly understood in agricultural soils. In this study, we evaluated the effects of more than 20 years of no-till farming (NTF) and conventional tillage (CT) on soil physicochemical properties, bacterial and fungal community composition, and inferred functions related to C and nutrient cycling in the Argentine Pampas. We show that NTF increased total organic carbon (TOC) stocks in surface soils and promoted edaphic conditions associated with C stabilization, including higher cation exchange capacity and structural stability. Bacterial communities exhibited high functional redundancy and were primarily structured along sodium-related parameters, whereas fungal communities were more sensitive to management, with NTF favoring ligninolytic and symbiotic fungi that contribute to necromass formation and long-term carbon stabilization. In contrast, CT enriched opportunistic fungal guilds associated with disturbance and short-term nutrient turnover. Phylogenetic analyses revealed community assembly dominated by environmental filtering in both microbial domains. Overall, these results highlight the central role of fungi as mediators of soil C stabilization and suggest that conservation practices such as NTF enhance microbiome contributions to ecosystem services and climate change mitigation in intensively managed agroecosystems.
EEA Manfredi
Fil: Gortari, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Gortari, Maximiliano. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Gortari, Maximiliano. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina
Fil: Maguire, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Área de Mejoramiento Genético Vegetal; Argentina
Fil: Ezquiaga, Juan Pedro. Provincia de Buenos Aires. Ministerio de Desarrollo Agrario (MDA). Chacra Experimental Integrada Chascomús; ArgentinaI
Fil: Cicchino, Mariano Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado. Agencia de Extensión Rural Chascomús; Argentina
Fil: Bailleres, Matias Andres. Provincia de Buenos Aires. Ministerio de Desarrollo Agrario (MDA). Chacra Experimental Integrada Chascomús; Argentina
Fil: Escaray, Roberto Ulises. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Escaray, Roberto Ulises. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Ruíz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Ruíz, Oscar Adolfo. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
Fil: Llames, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Ecología y Fotobiología Acuática; Argentina
Fil: Llames, María Eugenia. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina
description Soil microbial communities play a key role in carbon (C) cycling in agroecosystems; however, their long-term responses to contrasting management practices remain poorly understood in agricultural soils. In this study, we evaluated the effects of more than 20 years of no-till farming (NTF) and conventional tillage (CT) on soil physicochemical properties, bacterial and fungal community composition, and inferred functions related to C and nutrient cycling in the Argentine Pampas. We show that NTF increased total organic carbon (TOC) stocks in surface soils and promoted edaphic conditions associated with C stabilization, including higher cation exchange capacity and structural stability. Bacterial communities exhibited high functional redundancy and were primarily structured along sodium-related parameters, whereas fungal communities were more sensitive to management, with NTF favoring ligninolytic and symbiotic fungi that contribute to necromass formation and long-term carbon stabilization. In contrast, CT enriched opportunistic fungal guilds associated with disturbance and short-term nutrient turnover. Phylogenetic analyses revealed community assembly dominated by environmental filtering in both microbial domains. Overall, these results highlight the central role of fungi as mediators of soil C stabilization and suggest that conservation practices such as NTF enhance microbiome contributions to ecosystem services and climate change mitigation in intensively managed agroecosystems.
publishDate 2026
dc.date.none.fl_str_mv 2026-08-18T11:45:27Z
2026-08-18T11:45:27Z
2026-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27432
https://www.nature.com/articles/s41598-026-47621-4
2045-2322
https://doi.org/10.1038/s41598-026-47621-4
url http://hdl.handle.net/20.500.12123/27432
https://www.nature.com/articles/s41598-026-47621-4
https://doi.org/10.1038/s41598-026-47621-4
identifier_str_mv 2045-2322
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Scientific Reports 16 : Article number: 21484. (2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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