Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevines

Autores
Varela, Anabella; Marfil, Carlos Federico; Gomez Talquenca, Gonzalo; Fontana, Ariel; Asurmendi, Sebastian; Buscema, Fernando; Berli, Federico
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
DNA cytosine methylation, an epigenetic mechanism involved in gene regulation and genome stability, remains poorly understood in terms of its role under changing environmental conditions. Previous research using methylation-sensitive amplified polymorphism (MSAP) markers in a Vitis vinifera L. cv. Malbec clone showed vineyard-specific DNA methylation polymorphism, but no change in overall methylation levels. To complement these findings, the present study investigates the intra-seasonal epigenetic dynamics between genetically identical plants grown in different vineyards through a transplanting experiment. Cuttings of the same clone, showing differential methylation patterns imposed by the vineyard of origin (Agrelo and Gualtallary), were cultivated in a common vineyard (Lunlunta). Using high-performance liquid chromatography-ultraviolet detection, the quantification of global DNA 5-methylcytosine (5-mC) levels revealed relatively low overall 5-mC percentages in grapevines, with higher levels in Agrelo (5.8%) compared to Gualtallary plants (3.7%). The transplanted plants maintained the 5-mC levels differences between vineyards (9.8% vs 6.2%), which equalized in subsequent seasons (7.5% vs 7%). Additionally, the study examined 5-mC polymorphism using MSAP markers in Lunlunta transplanted plants over three seasons. The observed differences between vineyards in MSAP patterns during the initial growing season gradually diminished, suggesting a reprogramming of the hemimethylated pattern following implantation in the common vineyard. In contrast, the non-methylated pattern exhibited greater stability, indicating a potential memory effect. Overall, this study provides valuable insights into the dynamic nature of DNA methylation in grapevines under changing environmental conditions, with potential implications for crop management and breeding strategies.
EEA Mendoza
Fil: Varela, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Varela, Anabella. Universidad Nacional de Cuyo (UNCuyo). Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Marfil, Carlos Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Marfil, Carlos Federico. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias; Argentina
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fontana, Ariel. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Buscema, Fernando. Catena Institute of Wine; Argentina
Fil: Berli, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias; Argentina
Fuente
Plant Science 342 : 112037. (May 2024)
Materia
ADN
Citosina
Metilación
Trasplante
Vid
Reproducción Asexual
Plasticidad Fenotípica
Vitis vinifera
DNA
Cytosine
Methylation
Transplantation
Grapevines
Asexual Reproduction
Phenotypic Plasticity
Malbec
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27169
Acceder
id INTADig_39ededde18dbe9ef1a6d92920426782a
oai_identifier_str oai:localhost:20.500.12123/27169
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevinesVarela, AnabellaMarfil, Carlos FedericoGomez Talquenca, GonzaloFontana, ArielAsurmendi, SebastianBuscema, FernandoBerli, FedericoADNCitosinaMetilaciónTrasplanteVidReproducción AsexualPlasticidad FenotípicaVitis viniferaDNACytosineMethylationTransplantationGrapevinesAsexual ReproductionPhenotypic PlasticityMalbecDNA cytosine methylation, an epigenetic mechanism involved in gene regulation and genome stability, remains poorly understood in terms of its role under changing environmental conditions. Previous research using methylation-sensitive amplified polymorphism (MSAP) markers in a Vitis vinifera L. cv. Malbec clone showed vineyard-specific DNA methylation polymorphism, but no change in overall methylation levels. To complement these findings, the present study investigates the intra-seasonal epigenetic dynamics between genetically identical plants grown in different vineyards through a transplanting experiment. Cuttings of the same clone, showing differential methylation patterns imposed by the vineyard of origin (Agrelo and Gualtallary), were cultivated in a common vineyard (Lunlunta). Using high-performance liquid chromatography-ultraviolet detection, the quantification of global DNA 5-methylcytosine (5-mC) levels revealed relatively low overall 5-mC percentages in grapevines, with higher levels in Agrelo (5.8%) compared to Gualtallary plants (3.7%). The transplanted plants maintained the 5-mC levels differences between vineyards (9.8% vs 6.2%), which equalized in subsequent seasons (7.5% vs 7%). Additionally, the study examined 5-mC polymorphism using MSAP markers in Lunlunta transplanted plants over three seasons. The observed differences between vineyards in MSAP patterns during the initial growing season gradually diminished, suggesting a reprogramming of the hemimethylated pattern following implantation in the common vineyard. In contrast, the non-methylated pattern exhibited greater stability, indicating a potential memory effect. Overall, this study provides valuable insights into the dynamic nature of DNA methylation in grapevines under changing environmental conditions, with potential implications for crop management and breeding strategies.EEA MendozaFil: Varela, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Varela, Anabella. Universidad Nacional de Cuyo (UNCuyo). Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Marfil, Carlos Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Marfil, Carlos Federico. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias; ArgentinaFil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Fontana, Ariel. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Buscema, Fernando. Catena Institute of Wine; ArgentinaFil: Berli, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Berli, Federico. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias; ArgentinaElsevier2026-07-28T14:21:33Z2026-07-28T14:21:33Z2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27169https://www.sciencedirect.com/science/article/abs/pii/S0168945224000645?via%3Dihub0168-9452 (impreso)1873-2259 (en línea)https://doi.org/10.1016/j.plantsci.2024.112037Plant Science 342 : 112037. (May 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:36Zoai:localhost:20.500.12123/27169instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:36.694INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevines
title Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevines
spellingShingle Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevines
Varela, Anabella
ADN
Citosina
Metilación
Trasplante
Vid
Reproducción Asexual
Plasticidad Fenotípica
Vitis vinifera
DNA
Cytosine
Methylation
Transplantation
Grapevines
Asexual Reproduction
Phenotypic Plasticity
Malbec
title_short Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevines
title_full Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevines
title_fullStr Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevines
title_full_unstemmed Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevines
title_sort Three-year study of DNA cytosine methylation dynamics in transplanted Malbec grapevines
dc.creator.none.fl_str_mv Varela, Anabella
Marfil, Carlos Federico
Gomez Talquenca, Gonzalo
Fontana, Ariel
Asurmendi, Sebastian
Buscema, Fernando
Berli, Federico
author Varela, Anabella
author_facet Varela, Anabella
Marfil, Carlos Federico
Gomez Talquenca, Gonzalo
Fontana, Ariel
Asurmendi, Sebastian
Buscema, Fernando
Berli, Federico
author_role author
author2 Marfil, Carlos Federico
Gomez Talquenca, Gonzalo
Fontana, Ariel
Asurmendi, Sebastian
Buscema, Fernando
Berli, Federico
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ADN
Citosina
Metilación
Trasplante
Vid
Reproducción Asexual
Plasticidad Fenotípica
Vitis vinifera
DNA
Cytosine
Methylation
Transplantation
Grapevines
Asexual Reproduction
Phenotypic Plasticity
Malbec
topic ADN
Citosina
Metilación
Trasplante
Vid
Reproducción Asexual
Plasticidad Fenotípica
Vitis vinifera
DNA
Cytosine
Methylation
Transplantation
Grapevines
Asexual Reproduction
Phenotypic Plasticity
Malbec
dc.description.none.fl_txt_mv DNA cytosine methylation, an epigenetic mechanism involved in gene regulation and genome stability, remains poorly understood in terms of its role under changing environmental conditions. Previous research using methylation-sensitive amplified polymorphism (MSAP) markers in a Vitis vinifera L. cv. Malbec clone showed vineyard-specific DNA methylation polymorphism, but no change in overall methylation levels. To complement these findings, the present study investigates the intra-seasonal epigenetic dynamics between genetically identical plants grown in different vineyards through a transplanting experiment. Cuttings of the same clone, showing differential methylation patterns imposed by the vineyard of origin (Agrelo and Gualtallary), were cultivated in a common vineyard (Lunlunta). Using high-performance liquid chromatography-ultraviolet detection, the quantification of global DNA 5-methylcytosine (5-mC) levels revealed relatively low overall 5-mC percentages in grapevines, with higher levels in Agrelo (5.8%) compared to Gualtallary plants (3.7%). The transplanted plants maintained the 5-mC levels differences between vineyards (9.8% vs 6.2%), which equalized in subsequent seasons (7.5% vs 7%). Additionally, the study examined 5-mC polymorphism using MSAP markers in Lunlunta transplanted plants over three seasons. The observed differences between vineyards in MSAP patterns during the initial growing season gradually diminished, suggesting a reprogramming of the hemimethylated pattern following implantation in the common vineyard. In contrast, the non-methylated pattern exhibited greater stability, indicating a potential memory effect. Overall, this study provides valuable insights into the dynamic nature of DNA methylation in grapevines under changing environmental conditions, with potential implications for crop management and breeding strategies.
EEA Mendoza
Fil: Varela, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Varela, Anabella. Universidad Nacional de Cuyo (UNCuyo). Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Marfil, Carlos Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Marfil, Carlos Federico. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias; Argentina
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fontana, Ariel. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Buscema, Fernando. Catena Institute of Wine; Argentina
Fil: Berli, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias; Argentina
description DNA cytosine methylation, an epigenetic mechanism involved in gene regulation and genome stability, remains poorly understood in terms of its role under changing environmental conditions. Previous research using methylation-sensitive amplified polymorphism (MSAP) markers in a Vitis vinifera L. cv. Malbec clone showed vineyard-specific DNA methylation polymorphism, but no change in overall methylation levels. To complement these findings, the present study investigates the intra-seasonal epigenetic dynamics between genetically identical plants grown in different vineyards through a transplanting experiment. Cuttings of the same clone, showing differential methylation patterns imposed by the vineyard of origin (Agrelo and Gualtallary), were cultivated in a common vineyard (Lunlunta). Using high-performance liquid chromatography-ultraviolet detection, the quantification of global DNA 5-methylcytosine (5-mC) levels revealed relatively low overall 5-mC percentages in grapevines, with higher levels in Agrelo (5.8%) compared to Gualtallary plants (3.7%). The transplanted plants maintained the 5-mC levels differences between vineyards (9.8% vs 6.2%), which equalized in subsequent seasons (7.5% vs 7%). Additionally, the study examined 5-mC polymorphism using MSAP markers in Lunlunta transplanted plants over three seasons. The observed differences between vineyards in MSAP patterns during the initial growing season gradually diminished, suggesting a reprogramming of the hemimethylated pattern following implantation in the common vineyard. In contrast, the non-methylated pattern exhibited greater stability, indicating a potential memory effect. Overall, this study provides valuable insights into the dynamic nature of DNA methylation in grapevines under changing environmental conditions, with potential implications for crop management and breeding strategies.
publishDate 2024
dc.date.none.fl_str_mv 2024-05
2026-07-28T14:21:33Z
2026-07-28T14:21:33Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27169
https://www.sciencedirect.com/science/article/abs/pii/S0168945224000645?via%3Dihub
0168-9452 (impreso)
1873-2259 (en línea)
https://doi.org/10.1016/j.plantsci.2024.112037
url http://hdl.handle.net/20.500.12123/27169
https://www.sciencedirect.com/science/article/abs/pii/S0168945224000645?via%3Dihub
https://doi.org/10.1016/j.plantsci.2024.112037
identifier_str_mv 0168-9452 (impreso)
1873-2259 (en línea)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Plant Science 342 : 112037. (May 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1877227352006590464
score 12.733355

Publicaciones similares