First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural Modelling

Autores
Yanniccari, Marcos; Gigon, Ramon
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hirschfeldia incana is a widely distributed Brassicaceae species that has become a weed in temperate cropping systems worldwide. In the Argentine Pampas, its increasing prevalence in winter cereals has raised concerns because acetolactate synthase (ALS)-inhibiting herbicides are extensively used for broadleaf weed control. This study aimed to characterise the mechanism of resistance to ALS-inhibiting herbicides in two putatively metsulfuron-methyl-resistant H. incana populations and evaluate their cross-resistance patterns. This work constitutes the first report of ALS-inhibitor resistance in H. incana and provides the first ALS coding sequences available for this species. Sequencing identified the Trp-574-Leu substitution as the only non-synonymous mutation associated with resistance, while five synonymous polymorphisms distinguished the resistant populations, indicating two distinct resistant haplotypes. Whole-plant dose–response assays demonstrated broad cross-resistance to nine herbicides representing the five chemical families of ALS inhibitors, with resistance indices ranging from 10 to 22 and exceeding 31.6 for flucarbazone-sodium. Cross-resistance extended to the sulfonylaminocarbonyltriazolinone and pyrimidinyl benzoate families despite the absence of previous field exposure to either group. Molecular docking predicted reduced binding affinity of all evaluated herbicides towards the Trp-574-Leu ALS variant, although the magnitude of this effect varied among compounds and was accompanied by herbicide-specific changes in the ligand-binding environment, including loss and compensatory reorganisation of noncovalent interactions. These findings demonstrate that selection imposed by a limited number of ALS-inhibiting herbicide families can compromise the efficacy of chemically distinct ALS inhibitors, highlighting the need for integrated weed management programs based on diversification of herbicide sites of action across crop rotations.
EEA Barrow
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
Fuente
Weed Research 66 (5) : e70113. (September/October 2026)
Materia
Herbicidas
Resistencia a los Herbicidas
Malezas
Escarda
Herbicides
Herbicide Resistance
Weeds
Brassicaceae
Weed Control
Hirschfeldia incana
Control de Malezas
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/28176
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oai_identifier_str oai:localhost:20.500.12123/28176
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network_name_str INTA Digital (INTA)
spelling First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural ModellingYanniccari, MarcosGigon, RamonHerbicidasResistencia a los HerbicidasMalezasEscardaHerbicidesHerbicide ResistanceWeedsBrassicaceaeWeed ControlHirschfeldia incanaControl de MalezasHirschfeldia incana is a widely distributed Brassicaceae species that has become a weed in temperate cropping systems worldwide. In the Argentine Pampas, its increasing prevalence in winter cereals has raised concerns because acetolactate synthase (ALS)-inhibiting herbicides are extensively used for broadleaf weed control. This study aimed to characterise the mechanism of resistance to ALS-inhibiting herbicides in two putatively metsulfuron-methyl-resistant H. incana populations and evaluate their cross-resistance patterns. This work constitutes the first report of ALS-inhibitor resistance in H. incana and provides the first ALS coding sequences available for this species. Sequencing identified the Trp-574-Leu substitution as the only non-synonymous mutation associated with resistance, while five synonymous polymorphisms distinguished the resistant populations, indicating two distinct resistant haplotypes. Whole-plant dose–response assays demonstrated broad cross-resistance to nine herbicides representing the five chemical families of ALS inhibitors, with resistance indices ranging from 10 to 22 and exceeding 31.6 for flucarbazone-sodium. Cross-resistance extended to the sulfonylaminocarbonyltriazolinone and pyrimidinyl benzoate families despite the absence of previous field exposure to either group. Molecular docking predicted reduced binding affinity of all evaluated herbicides towards the Trp-574-Leu ALS variant, although the magnitude of this effect varied among compounds and was accompanied by herbicide-specific changes in the ligand-binding environment, including loss and compensatory reorganisation of noncovalent interactions. These findings demonstrate that selection imposed by a limited number of ALS-inhibiting herbicide families can compromise the efficacy of chemically distinct ALS inhibitors, highlighting the need for integrated weed management programs based on diversification of herbicide sites of action across crop rotations.EEA BarrowFil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaFil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Gigón, Ramón. Consultor privado en Control de Malezas; ArgentinaWiley2026-10-19T16:19:52Z2026-10-19T16:19:52Z2026-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/28176https://onlinelibrary.wiley.com/doi/10.1111/wre.701130043-17371365-3180https://doi.org/10.1111/wre.70113Weed Research 66 (5) : e70113. (September/October 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-11-05T11:51:41Zoai:localhost:20.500.12123/28176instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-11-05 11:51:41.669INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural Modelling
title First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural Modelling
spellingShingle First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural Modelling
Yanniccari, Marcos
Herbicidas
Resistencia a los Herbicidas
Malezas
Escarda
Herbicides
Herbicide Resistance
Weeds
Brassicaceae
Weed Control
Hirschfeldia incana
Control de Malezas
title_short First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural Modelling
title_full First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural Modelling
title_fullStr First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural Modelling
title_full_unstemmed First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural Modelling
title_sort First Evidence of Resistance to ALS-Inhibiting Herbicides in Hirschfeldia incana: Insights From Whole-Plant Responses and Structural Modelling
dc.creator.none.fl_str_mv Yanniccari, Marcos
Gigon, Ramon
author Yanniccari, Marcos
author_facet Yanniccari, Marcos
Gigon, Ramon
author_role author
author2 Gigon, Ramon
author2_role author
dc.subject.none.fl_str_mv Herbicidas
Resistencia a los Herbicidas
Malezas
Escarda
Herbicides
Herbicide Resistance
Weeds
Brassicaceae
Weed Control
Hirschfeldia incana
Control de Malezas
topic Herbicidas
Resistencia a los Herbicidas
Malezas
Escarda
Herbicides
Herbicide Resistance
Weeds
Brassicaceae
Weed Control
Hirschfeldia incana
Control de Malezas
dc.description.none.fl_txt_mv Hirschfeldia incana is a widely distributed Brassicaceae species that has become a weed in temperate cropping systems worldwide. In the Argentine Pampas, its increasing prevalence in winter cereals has raised concerns because acetolactate synthase (ALS)-inhibiting herbicides are extensively used for broadleaf weed control. This study aimed to characterise the mechanism of resistance to ALS-inhibiting herbicides in two putatively metsulfuron-methyl-resistant H. incana populations and evaluate their cross-resistance patterns. This work constitutes the first report of ALS-inhibitor resistance in H. incana and provides the first ALS coding sequences available for this species. Sequencing identified the Trp-574-Leu substitution as the only non-synonymous mutation associated with resistance, while five synonymous polymorphisms distinguished the resistant populations, indicating two distinct resistant haplotypes. Whole-plant dose–response assays demonstrated broad cross-resistance to nine herbicides representing the five chemical families of ALS inhibitors, with resistance indices ranging from 10 to 22 and exceeding 31.6 for flucarbazone-sodium. Cross-resistance extended to the sulfonylaminocarbonyltriazolinone and pyrimidinyl benzoate families despite the absence of previous field exposure to either group. Molecular docking predicted reduced binding affinity of all evaluated herbicides towards the Trp-574-Leu ALS variant, although the magnitude of this effect varied among compounds and was accompanied by herbicide-specific changes in the ligand-binding environment, including loss and compensatory reorganisation of noncovalent interactions. These findings demonstrate that selection imposed by a limited number of ALS-inhibiting herbicide families can compromise the efficacy of chemically distinct ALS inhibitors, highlighting the need for integrated weed management programs based on diversification of herbicide sites of action across crop rotations.
EEA Barrow
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
description Hirschfeldia incana is a widely distributed Brassicaceae species that has become a weed in temperate cropping systems worldwide. In the Argentine Pampas, its increasing prevalence in winter cereals has raised concerns because acetolactate synthase (ALS)-inhibiting herbicides are extensively used for broadleaf weed control. This study aimed to characterise the mechanism of resistance to ALS-inhibiting herbicides in two putatively metsulfuron-methyl-resistant H. incana populations and evaluate their cross-resistance patterns. This work constitutes the first report of ALS-inhibitor resistance in H. incana and provides the first ALS coding sequences available for this species. Sequencing identified the Trp-574-Leu substitution as the only non-synonymous mutation associated with resistance, while five synonymous polymorphisms distinguished the resistant populations, indicating two distinct resistant haplotypes. Whole-plant dose–response assays demonstrated broad cross-resistance to nine herbicides representing the five chemical families of ALS inhibitors, with resistance indices ranging from 10 to 22 and exceeding 31.6 for flucarbazone-sodium. Cross-resistance extended to the sulfonylaminocarbonyltriazolinone and pyrimidinyl benzoate families despite the absence of previous field exposure to either group. Molecular docking predicted reduced binding affinity of all evaluated herbicides towards the Trp-574-Leu ALS variant, although the magnitude of this effect varied among compounds and was accompanied by herbicide-specific changes in the ligand-binding environment, including loss and compensatory reorganisation of noncovalent interactions. These findings demonstrate that selection imposed by a limited number of ALS-inhibiting herbicide families can compromise the efficacy of chemically distinct ALS inhibitors, highlighting the need for integrated weed management programs based on diversification of herbicide sites of action across crop rotations.
publishDate 2026
dc.date.none.fl_str_mv 2026-10-19T16:19:52Z
2026-10-19T16:19:52Z
2026-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/28176
https://onlinelibrary.wiley.com/doi/10.1111/wre.70113
0043-1737
1365-3180
https://doi.org/10.1111/wre.70113
url http://hdl.handle.net/20.500.12123/28176
https://onlinelibrary.wiley.com/doi/10.1111/wre.70113
https://doi.org/10.1111/wre.70113
identifier_str_mv 0043-1737
1365-3180
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Weed Research 66 (5) : e70113. (September/October 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.334379

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