The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars

Autores
Murguía, José Pablo; Curin, Facundo; Chan, Raquel Lía; Gonzalez, Fernanda Gabriela
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The sunflower gene HaHB4 was previously described as conferring drought tolerance to Cadenza wheat plants, as assessed in 37 field trials. HaHB4-wheat is the first transgenic wheat trait approved for global markets. In this work, we show that transgenic plants also exhibit heat tolerance in the pre-anthesis stage. During 2023 and 2024, the experiments were conducted in open-air conditions, using the modern cultivars Algarrobo and Feroz by introgression of the original Cadenza HaHB4. The newly obtained transgenic wheat plants, particularly Algarrobo, as well as the original Cadenza HaHB4, maintained stabilized grain yields under high-temperature stress applied at the pre-anthesis stage, whereas wild-type genotypes suffered severe productivity losses. This yield advantage was primarily driven by a higher grain number rather than grain weight. Interestingly, heat stress applied after anthesis showed no significant difference impact in yield, identifying pre-anthesis as the vital window for HaHB4 efficacy. HaHB4 plants exhibited a higher harvest index and a greater number of fertile florets and spikelets per spike, suggesting that the transgene protects the reproductive potential during early development. At the molecular level, HaHB4 wheat appears constitutively prepared for stress. Transcript levels analysis showed the differential regulation of heat shock proteins and stress-responsive transcription factors even before the onset of heat. Furthermore, the stability of transgenic plants was notably higher, showing significantly lower coefficients of variation across yield components compared to wild-type counterparts. This increased stability suggests that HaHB4 acts as a buffer against environmental variability, ensuring more predictable harvests under fluctuating thermal conditions.
EEA Pergamino
Fil: Murguía, José. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Murguía, José. Universidad Nacional del Litoral (UNL). Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Curín, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigaciones y Transferencias del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Curín, Facundo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA); Argentina
Fil: Chan, Raquel L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Chan, Raquel Lía. Universidad Nacional del Litoral (UNL). Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: González, Fernanda G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Ecofisiología; Argentina
Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
Fil: González, Fernanda G. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología del Litoral; Argentina
Fil: González, Fernanda G. Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA); Argentina
Fuente
Plant Physiology and Biochemistry 234: 111355. (May 2026)
Materia
Trigo
Rendimiento de Cultivos
Estrés Térmico
Tolerancia al Calor
Wheat
Crop Yield
Heat Stress
Heat Tolerance
Wheat HaHB4
Algarrobo Wheat
Feroz Wheat
Grain Yields
Grain Set
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27086
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27086
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivarsMurguía, José PabloCurin, FacundoChan, Raquel LíaGonzalez, Fernanda GabrielaTrigoRendimiento de CultivosEstrés TérmicoTolerancia al CalorWheatCrop YieldHeat StressHeat ToleranceWheat HaHB4Algarrobo WheatFeroz WheatGrain YieldsGrain SetThe sunflower gene HaHB4 was previously described as conferring drought tolerance to Cadenza wheat plants, as assessed in 37 field trials. HaHB4-wheat is the first transgenic wheat trait approved for global markets. In this work, we show that transgenic plants also exhibit heat tolerance in the pre-anthesis stage. During 2023 and 2024, the experiments were conducted in open-air conditions, using the modern cultivars Algarrobo and Feroz by introgression of the original Cadenza HaHB4. The newly obtained transgenic wheat plants, particularly Algarrobo, as well as the original Cadenza HaHB4, maintained stabilized grain yields under high-temperature stress applied at the pre-anthesis stage, whereas wild-type genotypes suffered severe productivity losses. This yield advantage was primarily driven by a higher grain number rather than grain weight. Interestingly, heat stress applied after anthesis showed no significant difference impact in yield, identifying pre-anthesis as the vital window for HaHB4 efficacy. HaHB4 plants exhibited a higher harvest index and a greater number of fertile florets and spikelets per spike, suggesting that the transgene protects the reproductive potential during early development. At the molecular level, HaHB4 wheat appears constitutively prepared for stress. Transcript levels analysis showed the differential regulation of heat shock proteins and stress-responsive transcription factors even before the onset of heat. Furthermore, the stability of transgenic plants was notably higher, showing significantly lower coefficients of variation across yield components compared to wild-type counterparts. This increased stability suggests that HaHB4 acts as a buffer against environmental variability, ensuring more predictable harvests under fluctuating thermal conditions.EEA PergaminoFil: Murguía, José. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Murguía, José. Universidad Nacional del Litoral (UNL). Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Curín, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigaciones y Transferencias del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Curín, Facundo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA); ArgentinaFil: Chan, Raquel L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Chan, Raquel Lía. Universidad Nacional del Litoral (UNL). Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: González, Fernanda G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Ecofisiología; ArgentinaFil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; ArgentinaFil: González, Fernanda G. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología del Litoral; ArgentinaFil: González, Fernanda G. Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA); ArgentinaElsevier2026-07-21T17:32:13Z2026-07-21T17:32:13Z2026-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27086https://www.sciencedirect.com/science/article/pii/S09819428260034140981-9428https://doi.org/10.1016/j.plaphy.2026.111355Plant Physiology and Biochemistry 234: 111355. (May 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I102, Bases ecofisiológicas para mejorar la adaptación de los cultivos a la variabilidad ambiental mediante el manejo agronómico y el mejoramiento genéticoinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:29Zoai:localhost:20.500.12123/27086instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:30.513INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars
title The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars
spellingShingle The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars
Murguía, José Pablo
Trigo
Rendimiento de Cultivos
Estrés Térmico
Tolerancia al Calor
Wheat
Crop Yield
Heat Stress
Heat Tolerance
Wheat HaHB4
Algarrobo Wheat
Feroz Wheat
Grain Yields
Grain Set
title_short The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars
title_full The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars
title_fullStr The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars
title_full_unstemmed The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars
title_sort The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars
dc.creator.none.fl_str_mv Murguía, José Pablo
Curin, Facundo
Chan, Raquel Lía
Gonzalez, Fernanda Gabriela
author Murguía, José Pablo
author_facet Murguía, José Pablo
Curin, Facundo
Chan, Raquel Lía
Gonzalez, Fernanda Gabriela
author_role author
author2 Curin, Facundo
Chan, Raquel Lía
Gonzalez, Fernanda Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv Trigo
Rendimiento de Cultivos
Estrés Térmico
Tolerancia al Calor
Wheat
Crop Yield
Heat Stress
Heat Tolerance
Wheat HaHB4
Algarrobo Wheat
Feroz Wheat
Grain Yields
Grain Set
topic Trigo
Rendimiento de Cultivos
Estrés Térmico
Tolerancia al Calor
Wheat
Crop Yield
Heat Stress
Heat Tolerance
Wheat HaHB4
Algarrobo Wheat
Feroz Wheat
Grain Yields
Grain Set
dc.description.none.fl_txt_mv The sunflower gene HaHB4 was previously described as conferring drought tolerance to Cadenza wheat plants, as assessed in 37 field trials. HaHB4-wheat is the first transgenic wheat trait approved for global markets. In this work, we show that transgenic plants also exhibit heat tolerance in the pre-anthesis stage. During 2023 and 2024, the experiments were conducted in open-air conditions, using the modern cultivars Algarrobo and Feroz by introgression of the original Cadenza HaHB4. The newly obtained transgenic wheat plants, particularly Algarrobo, as well as the original Cadenza HaHB4, maintained stabilized grain yields under high-temperature stress applied at the pre-anthesis stage, whereas wild-type genotypes suffered severe productivity losses. This yield advantage was primarily driven by a higher grain number rather than grain weight. Interestingly, heat stress applied after anthesis showed no significant difference impact in yield, identifying pre-anthesis as the vital window for HaHB4 efficacy. HaHB4 plants exhibited a higher harvest index and a greater number of fertile florets and spikelets per spike, suggesting that the transgene protects the reproductive potential during early development. At the molecular level, HaHB4 wheat appears constitutively prepared for stress. Transcript levels analysis showed the differential regulation of heat shock proteins and stress-responsive transcription factors even before the onset of heat. Furthermore, the stability of transgenic plants was notably higher, showing significantly lower coefficients of variation across yield components compared to wild-type counterparts. This increased stability suggests that HaHB4 acts as a buffer against environmental variability, ensuring more predictable harvests under fluctuating thermal conditions.
EEA Pergamino
Fil: Murguía, José. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Murguía, José. Universidad Nacional del Litoral (UNL). Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Curín, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigaciones y Transferencias del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Curín, Facundo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA); Argentina
Fil: Chan, Raquel L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Chan, Raquel Lía. Universidad Nacional del Litoral (UNL). Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: González, Fernanda G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Ecofisiología; Argentina
Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
Fil: González, Fernanda G. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología del Litoral; Argentina
Fil: González, Fernanda G. Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA); Argentina
description The sunflower gene HaHB4 was previously described as conferring drought tolerance to Cadenza wheat plants, as assessed in 37 field trials. HaHB4-wheat is the first transgenic wheat trait approved for global markets. In this work, we show that transgenic plants also exhibit heat tolerance in the pre-anthesis stage. During 2023 and 2024, the experiments were conducted in open-air conditions, using the modern cultivars Algarrobo and Feroz by introgression of the original Cadenza HaHB4. The newly obtained transgenic wheat plants, particularly Algarrobo, as well as the original Cadenza HaHB4, maintained stabilized grain yields under high-temperature stress applied at the pre-anthesis stage, whereas wild-type genotypes suffered severe productivity losses. This yield advantage was primarily driven by a higher grain number rather than grain weight. Interestingly, heat stress applied after anthesis showed no significant difference impact in yield, identifying pre-anthesis as the vital window for HaHB4 efficacy. HaHB4 plants exhibited a higher harvest index and a greater number of fertile florets and spikelets per spike, suggesting that the transgene protects the reproductive potential during early development. At the molecular level, HaHB4 wheat appears constitutively prepared for stress. Transcript levels analysis showed the differential regulation of heat shock proteins and stress-responsive transcription factors even before the onset of heat. Furthermore, the stability of transgenic plants was notably higher, showing significantly lower coefficients of variation across yield components compared to wild-type counterparts. This increased stability suggests that HaHB4 acts as a buffer against environmental variability, ensuring more predictable harvests under fluctuating thermal conditions.
publishDate 2026
dc.date.none.fl_str_mv 2026-07-21T17:32:13Z
2026-07-21T17:32:13Z
2026-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27086
https://www.sciencedirect.com/science/article/pii/S0981942826003414
0981-9428
https://doi.org/10.1016/j.plaphy.2026.111355
url http://hdl.handle.net/20.500.12123/27086
https://www.sciencedirect.com/science/article/pii/S0981942826003414
https://doi.org/10.1016/j.plaphy.2026.111355
identifier_str_mv 0981-9428
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I102, Bases ecofisiológicas para mejorar la adaptación de los cultivos a la variabilidad ambiental mediante el manejo agronómico y el mejoramiento genético
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Plant Physiology and Biochemistry 234: 111355. (May 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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