Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from Wheat

Autores
Di Pane, Francisco; Yanniccari, Marcos
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Across diverse agroecological environments worldwide, triticale provides economically competitive grain yields, high nutritional quality comparable to wheat, tolerance to drought and poor soil conditions, and resistance to several diseases commonly affecting rye. Despite its growing importance, triticale production relies largely on agronomic practices similar to those used in wheat. However, its greater sensitivity to several herbicides commonly used in wheat complicates weed management, making the development of herbicide-resistant triticale cultivars an important opportunity to facilitate broader adoption of this crop. The objective of this study was to develop triticale lines resistant to imidazolinone herbicides through introgression of resistance from a mutant Triticum aestivum line generated by 60Co gamma irradiation. Mutant wheat plants were treated with the recommended field rate of imazamox–imazapyr at the tillering stage, and a surviving plant was used as the source of imidazolinone resistance. This mutant was crossed with a triticale breeding line used as the female parent. The progeny was advanced to the F5 generation and treated with imazamox–imazapyr to confirm resistance. This breeding and selection process resulted in the stabilized triticale lines 5202 and 5195. Partial sequencing of the AHAS gene revealed a Ser-653-Asn substitution associated with imazamox–imazapyr resistance in both lines. Allele-specific sequencing further revealed contrasting AHAS sequence signatures between the resistant lines, suggesting differences in the genomic distribution of the resistance allele. Both resistant triticale lines survived the recommended field rate of imazamox–imazapyr applied either pre- or post-emergence, although they differed significantly in their response to the herbicide, with line 5195 consistently exhibiting the highest level of resistance. These findings highlight the value of induced mutagenesis as a source of novel agronomic variation for cereal breeding. Beyond facilitating direct weed control, these first imidazolinone-resistant triticale lines may provide an important agronomic advantage in crop rotations where residual imidazolinone activity limits the establishment of winter cereals.
EEA Barrow
Fil: Di Pane, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fuente
Crops 6 (6) : 100. (2026)
Materia
Trigo
Resistencia a los Herbicidas
Herbicida con Imidazolinona
Imazapir
Mutación Inducida
Wheat
Herbicide Resistance
Imidazolinone Herbicides
Imazapyr
Induced Mutation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/28270
Acceder
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oai_identifier_str oai:localhost:20.500.12123/28270
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spelling Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from WheatDi Pane, FranciscoYanniccari, MarcosTrigoResistencia a los HerbicidasHerbicida con ImidazolinonaImazapirMutación InducidaWheatHerbicide ResistanceImidazolinone HerbicidesImazapyrInduced MutationAcross diverse agroecological environments worldwide, triticale provides economically competitive grain yields, high nutritional quality comparable to wheat, tolerance to drought and poor soil conditions, and resistance to several diseases commonly affecting rye. Despite its growing importance, triticale production relies largely on agronomic practices similar to those used in wheat. However, its greater sensitivity to several herbicides commonly used in wheat complicates weed management, making the development of herbicide-resistant triticale cultivars an important opportunity to facilitate broader adoption of this crop. The objective of this study was to develop triticale lines resistant to imidazolinone herbicides through introgression of resistance from a mutant Triticum aestivum line generated by 60Co gamma irradiation. Mutant wheat plants were treated with the recommended field rate of imazamox–imazapyr at the tillering stage, and a surviving plant was used as the source of imidazolinone resistance. This mutant was crossed with a triticale breeding line used as the female parent. The progeny was advanced to the F5 generation and treated with imazamox–imazapyr to confirm resistance. This breeding and selection process resulted in the stabilized triticale lines 5202 and 5195. Partial sequencing of the AHAS gene revealed a Ser-653-Asn substitution associated with imazamox–imazapyr resistance in both lines. Allele-specific sequencing further revealed contrasting AHAS sequence signatures between the resistant lines, suggesting differences in the genomic distribution of the resistance allele. Both resistant triticale lines survived the recommended field rate of imazamox–imazapyr applied either pre- or post-emergence, although they differed significantly in their response to the herbicide, with line 5195 consistently exhibiting the highest level of resistance. These findings highlight the value of induced mutagenesis as a source of novel agronomic variation for cereal breeding. Beyond facilitating direct weed control, these first imidazolinone-resistant triticale lines may provide an important agronomic advantage in crop rotations where residual imidazolinone activity limits the establishment of winter cereals.EEA BarrowFil: Di Pane, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaFil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaMDPI2026-10-27T14:55:11Z2026-10-27T14:55:11Z2026-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/28270https://www.mdpi.com/2673-7655/6/6/1002673-7655https://doi.org/10.3390/crops6060100Crops 6 (6) : 100. (2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-11-05T11:51:42Zoai:localhost:20.500.12123/28270instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-11-05 11:51:42.546INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from Wheat
title Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from Wheat
spellingShingle Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from Wheat
Di Pane, Francisco
Trigo
Resistencia a los Herbicidas
Herbicida con Imidazolinona
Imazapir
Mutación Inducida
Wheat
Herbicide Resistance
Imidazolinone Herbicides
Imazapyr
Induced Mutation
title_short Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from Wheat
title_full Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from Wheat
title_fullStr Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from Wheat
title_full_unstemmed Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from Wheat
title_sort Development of the First Imidazolinone-Resistant Triticale Through Introgression of Mutant AHAS Alleles from Wheat
dc.creator.none.fl_str_mv Di Pane, Francisco
Yanniccari, Marcos
author Di Pane, Francisco
author_facet Di Pane, Francisco
Yanniccari, Marcos
author_role author
author2 Yanniccari, Marcos
author2_role author
dc.subject.none.fl_str_mv Trigo
Resistencia a los Herbicidas
Herbicida con Imidazolinona
Imazapir
Mutación Inducida
Wheat
Herbicide Resistance
Imidazolinone Herbicides
Imazapyr
Induced Mutation
topic Trigo
Resistencia a los Herbicidas
Herbicida con Imidazolinona
Imazapir
Mutación Inducida
Wheat
Herbicide Resistance
Imidazolinone Herbicides
Imazapyr
Induced Mutation
dc.description.none.fl_txt_mv Across diverse agroecological environments worldwide, triticale provides economically competitive grain yields, high nutritional quality comparable to wheat, tolerance to drought and poor soil conditions, and resistance to several diseases commonly affecting rye. Despite its growing importance, triticale production relies largely on agronomic practices similar to those used in wheat. However, its greater sensitivity to several herbicides commonly used in wheat complicates weed management, making the development of herbicide-resistant triticale cultivars an important opportunity to facilitate broader adoption of this crop. The objective of this study was to develop triticale lines resistant to imidazolinone herbicides through introgression of resistance from a mutant Triticum aestivum line generated by 60Co gamma irradiation. Mutant wheat plants were treated with the recommended field rate of imazamox–imazapyr at the tillering stage, and a surviving plant was used as the source of imidazolinone resistance. This mutant was crossed with a triticale breeding line used as the female parent. The progeny was advanced to the F5 generation and treated with imazamox–imazapyr to confirm resistance. This breeding and selection process resulted in the stabilized triticale lines 5202 and 5195. Partial sequencing of the AHAS gene revealed a Ser-653-Asn substitution associated with imazamox–imazapyr resistance in both lines. Allele-specific sequencing further revealed contrasting AHAS sequence signatures between the resistant lines, suggesting differences in the genomic distribution of the resistance allele. Both resistant triticale lines survived the recommended field rate of imazamox–imazapyr applied either pre- or post-emergence, although they differed significantly in their response to the herbicide, with line 5195 consistently exhibiting the highest level of resistance. These findings highlight the value of induced mutagenesis as a source of novel agronomic variation for cereal breeding. Beyond facilitating direct weed control, these first imidazolinone-resistant triticale lines may provide an important agronomic advantage in crop rotations where residual imidazolinone activity limits the establishment of winter cereals.
EEA Barrow
Fil: Di Pane, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
description Across diverse agroecological environments worldwide, triticale provides economically competitive grain yields, high nutritional quality comparable to wheat, tolerance to drought and poor soil conditions, and resistance to several diseases commonly affecting rye. Despite its growing importance, triticale production relies largely on agronomic practices similar to those used in wheat. However, its greater sensitivity to several herbicides commonly used in wheat complicates weed management, making the development of herbicide-resistant triticale cultivars an important opportunity to facilitate broader adoption of this crop. The objective of this study was to develop triticale lines resistant to imidazolinone herbicides through introgression of resistance from a mutant Triticum aestivum line generated by 60Co gamma irradiation. Mutant wheat plants were treated with the recommended field rate of imazamox–imazapyr at the tillering stage, and a surviving plant was used as the source of imidazolinone resistance. This mutant was crossed with a triticale breeding line used as the female parent. The progeny was advanced to the F5 generation and treated with imazamox–imazapyr to confirm resistance. This breeding and selection process resulted in the stabilized triticale lines 5202 and 5195. Partial sequencing of the AHAS gene revealed a Ser-653-Asn substitution associated with imazamox–imazapyr resistance in both lines. Allele-specific sequencing further revealed contrasting AHAS sequence signatures between the resistant lines, suggesting differences in the genomic distribution of the resistance allele. Both resistant triticale lines survived the recommended field rate of imazamox–imazapyr applied either pre- or post-emergence, although they differed significantly in their response to the herbicide, with line 5195 consistently exhibiting the highest level of resistance. These findings highlight the value of induced mutagenesis as a source of novel agronomic variation for cereal breeding. Beyond facilitating direct weed control, these first imidazolinone-resistant triticale lines may provide an important agronomic advantage in crop rotations where residual imidazolinone activity limits the establishment of winter cereals.
publishDate 2026
dc.date.none.fl_str_mv 2026-10-27T14:55:11Z
2026-10-27T14:55:11Z
2026-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/28270
https://www.mdpi.com/2673-7655/6/6/100
2673-7655
https://doi.org/10.3390/crops6060100
url http://hdl.handle.net/20.500.12123/28270
https://www.mdpi.com/2673-7655/6/6/100
https://doi.org/10.3390/crops6060100
identifier_str_mv 2673-7655
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Crops 6 (6) : 100. (2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.334379

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