Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract

Autores
Mate, Laura; Alvarez, Luis Ignacio; Lloberas, María Mercedes; Imperiale, Fernanda; Lanusse, Carlos Edmundo; Liron, Juan Pedro
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The economic impact of gastrointestinal (GI) nematode infections on livestock production iswell documented worldwide. Increasing evidence supports the hypothesis that parasite colonization induces significant changes in the GI tract environment and, therefore, in the landscape where the microbiota and parasites occur. Understanding the interactions betweenbacterial and parasite populations in the digestive tract of livestock may be useful to designparasite control strategies based on microbiota modification. The aims of this work were toinvestigate the impact of the oxytetracycline-mediated manipulation of the gut microbialcommunity on the composition of GI nematode populations in naturally infected sheep andto explore changes in the GI microbial communities after nematode population treatmentwith the anthelmintic compound monepantel. Extensive manipulation of the GI microbiotawith a therapeutic dose of the long-acting oxytetracycline formulation did not induce significant changes in the GI nematode burden. The gut microbiota of treated animals returned tocontrol levels 17 days after treatment, suggesting strong resilience of the sheep microbialcommunity to antibiotic-mediated microbiota perturbation. A significant decrease of the bacterial Mycoplasmataceae family (Log2FC = -4, Padj = 0.001) and a marked increase of theMethanobacteriaceae family (Log2FC = 2.9, Padj = 0.018) were observed in the abomasumof sheep receiving the monepantel treatment. While a comprehensive evaluation of theinteractions among GI mycoplasma, methanobacteria and nematode populations deservesfurther assessment, the bacteria-nematode population interactions should be included infuture control programs in livestock production. Understanding how bacteria and parasitesmay influence each other in the GI tract environment may substantially contribute to theknowledge of the role of microbiota composition in nematode parasite establishment andthe role of the parasites in the microbiota composition.
EEA Balcarce
Fil: Mate, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Mate, Laura. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Mate, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Alvarez, Luis Ignacio. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Alvarez, Luis Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: LLoberas, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Imperiale, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Imperiale, Fernanda. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Imperiale, Fernanda. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lanusse, Carlos Edmundo. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lanusse, Carlos Edmundo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lirón, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentin
Fil: Lirón, Juan Pedro. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lirón, Juan Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fuente
Plos One 19 (6) : e0306390
Materia
Microbial Flora
Nematodes
Sheep
Flora Microbiana
Nematodos
Ovinos
Bacterial Microbiota
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27469
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27469
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tractMate, LauraAlvarez, Luis IgnacioLloberas, María MercedesImperiale, FernandaLanusse, Carlos EdmundoLiron, Juan PedroMicrobial FloraNematodesSheepFlora MicrobianaNematodosOvinosBacterial MicrobiotaThe economic impact of gastrointestinal (GI) nematode infections on livestock production iswell documented worldwide. Increasing evidence supports the hypothesis that parasite colonization induces significant changes in the GI tract environment and, therefore, in the landscape where the microbiota and parasites occur. Understanding the interactions betweenbacterial and parasite populations in the digestive tract of livestock may be useful to designparasite control strategies based on microbiota modification. The aims of this work were toinvestigate the impact of the oxytetracycline-mediated manipulation of the gut microbialcommunity on the composition of GI nematode populations in naturally infected sheep andto explore changes in the GI microbial communities after nematode population treatmentwith the anthelmintic compound monepantel. Extensive manipulation of the GI microbiotawith a therapeutic dose of the long-acting oxytetracycline formulation did not induce significant changes in the GI nematode burden. The gut microbiota of treated animals returned tocontrol levels 17 days after treatment, suggesting strong resilience of the sheep microbialcommunity to antibiotic-mediated microbiota perturbation. A significant decrease of the bacterial Mycoplasmataceae family (Log2FC = -4, Padj = 0.001) and a marked increase of theMethanobacteriaceae family (Log2FC = 2.9, Padj = 0.018) were observed in the abomasumof sheep receiving the monepantel treatment. While a comprehensive evaluation of theinteractions among GI mycoplasma, methanobacteria and nematode populations deservesfurther assessment, the bacteria-nematode population interactions should be included infuture control programs in livestock production. Understanding how bacteria and parasitesmay influence each other in the GI tract environment may substantially contribute to theknowledge of the role of microbiota composition in nematode parasite establishment andthe role of the parasites in the microbiota composition.EEA BalcarceFil: Mate, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Mate, Laura. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Mate, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Alvarez, Luis Ignacio. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Alvarez, Luis Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: LLoberas, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); ArgentinaFil: Imperiale, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Imperiale, Fernanda. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Imperiale, Fernanda. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Lanusse, Carlos Edmundo. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Lanusse, Carlos Edmundo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Lirón, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinFil: Lirón, Juan Pedro. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaFil: Lirón, Juan Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; ArgentinaPublic Library of Science2026-08-19T13:17:54Z2026-08-19T13:17:54Z2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27469https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306390Mate L, Alvarez LI, Lloberas M, Imperiale F, Lanusse CE, Liron JP (2024) Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract. PLoS ONE 19(6): e0306390. https://doi.org/10.1371/ journal.pone.03063901932-6203https://doi.org/10.1371/ journal.pone.0306390Plos One 19 (6) : e0306390reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:43Zoai:localhost:20.500.12123/27469instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:44.087INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract
title Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract
spellingShingle Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract
Mate, Laura
Microbial Flora
Nematodes
Sheep
Flora Microbiana
Nematodos
Ovinos
Bacterial Microbiota
title_short Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract
title_full Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract
title_fullStr Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract
title_full_unstemmed Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract
title_sort Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract
dc.creator.none.fl_str_mv Mate, Laura
Alvarez, Luis Ignacio
Lloberas, María Mercedes
Imperiale, Fernanda
Lanusse, Carlos Edmundo
Liron, Juan Pedro
author Mate, Laura
author_facet Mate, Laura
Alvarez, Luis Ignacio
Lloberas, María Mercedes
Imperiale, Fernanda
Lanusse, Carlos Edmundo
Liron, Juan Pedro
author_role author
author2 Alvarez, Luis Ignacio
Lloberas, María Mercedes
Imperiale, Fernanda
Lanusse, Carlos Edmundo
Liron, Juan Pedro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Microbial Flora
Nematodes
Sheep
Flora Microbiana
Nematodos
Ovinos
Bacterial Microbiota
topic Microbial Flora
Nematodes
Sheep
Flora Microbiana
Nematodos
Ovinos
Bacterial Microbiota
dc.description.none.fl_txt_mv The economic impact of gastrointestinal (GI) nematode infections on livestock production iswell documented worldwide. Increasing evidence supports the hypothesis that parasite colonization induces significant changes in the GI tract environment and, therefore, in the landscape where the microbiota and parasites occur. Understanding the interactions betweenbacterial and parasite populations in the digestive tract of livestock may be useful to designparasite control strategies based on microbiota modification. The aims of this work were toinvestigate the impact of the oxytetracycline-mediated manipulation of the gut microbialcommunity on the composition of GI nematode populations in naturally infected sheep andto explore changes in the GI microbial communities after nematode population treatmentwith the anthelmintic compound monepantel. Extensive manipulation of the GI microbiotawith a therapeutic dose of the long-acting oxytetracycline formulation did not induce significant changes in the GI nematode burden. The gut microbiota of treated animals returned tocontrol levels 17 days after treatment, suggesting strong resilience of the sheep microbialcommunity to antibiotic-mediated microbiota perturbation. A significant decrease of the bacterial Mycoplasmataceae family (Log2FC = -4, Padj = 0.001) and a marked increase of theMethanobacteriaceae family (Log2FC = 2.9, Padj = 0.018) were observed in the abomasumof sheep receiving the monepantel treatment. While a comprehensive evaluation of theinteractions among GI mycoplasma, methanobacteria and nematode populations deservesfurther assessment, the bacteria-nematode population interactions should be included infuture control programs in livestock production. Understanding how bacteria and parasitesmay influence each other in the GI tract environment may substantially contribute to theknowledge of the role of microbiota composition in nematode parasite establishment andthe role of the parasites in the microbiota composition.
EEA Balcarce
Fil: Mate, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Mate, Laura. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Mate, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Alvarez, Luis Ignacio. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Alvarez, Luis Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: LLoberas, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Imperiale, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Imperiale, Fernanda. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Imperiale, Fernanda. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lanusse, Carlos Edmundo. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lanusse, Carlos Edmundo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lirón, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentin
Fil: Lirón, Juan Pedro. Centro Científico Tecnológico (CIC). Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
Fil: Lirón, Juan Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina
description The economic impact of gastrointestinal (GI) nematode infections on livestock production iswell documented worldwide. Increasing evidence supports the hypothesis that parasite colonization induces significant changes in the GI tract environment and, therefore, in the landscape where the microbiota and parasites occur. Understanding the interactions betweenbacterial and parasite populations in the digestive tract of livestock may be useful to designparasite control strategies based on microbiota modification. The aims of this work were toinvestigate the impact of the oxytetracycline-mediated manipulation of the gut microbialcommunity on the composition of GI nematode populations in naturally infected sheep andto explore changes in the GI microbial communities after nematode population treatmentwith the anthelmintic compound monepantel. Extensive manipulation of the GI microbiotawith a therapeutic dose of the long-acting oxytetracycline formulation did not induce significant changes in the GI nematode burden. The gut microbiota of treated animals returned tocontrol levels 17 days after treatment, suggesting strong resilience of the sheep microbialcommunity to antibiotic-mediated microbiota perturbation. A significant decrease of the bacterial Mycoplasmataceae family (Log2FC = -4, Padj = 0.001) and a marked increase of theMethanobacteriaceae family (Log2FC = 2.9, Padj = 0.018) were observed in the abomasumof sheep receiving the monepantel treatment. While a comprehensive evaluation of theinteractions among GI mycoplasma, methanobacteria and nematode populations deservesfurther assessment, the bacteria-nematode population interactions should be included infuture control programs in livestock production. Understanding how bacteria and parasitesmay influence each other in the GI tract environment may substantially contribute to theknowledge of the role of microbiota composition in nematode parasite establishment andthe role of the parasites in the microbiota composition.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
2026-08-19T13:17:54Z
2026-08-19T13:17:54Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27469
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306390
Mate L, Alvarez LI, Lloberas M, Imperiale F, Lanusse CE, Liron JP (2024) Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract. PLoS ONE 19(6): e0306390. https://doi.org/10.1371/ journal.pone.0306390
1932-6203
https://doi.org/10.1371/ journal.pone.0306390
url http://hdl.handle.net/20.500.12123/27469
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306390
https://doi.org/10.1371/ journal.pone.0306390
identifier_str_mv Mate L, Alvarez LI, Lloberas M, Imperiale F, Lanusse CE, Liron JP (2024) Interaction between bacterial microbiota and nematode parasite communities in sheep’s gastrointestinal tract. PLoS ONE 19(6): e0306390. https://doi.org/10.1371/ journal.pone.0306390
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv Plos One 19 (6) : e0306390
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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