Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives

Autores
Morrell, Eleonora Lidia; Navarro, Mauricio A.; Garcia, Jorge P.; Beingesser, Juliann; Uzal, Francisco A.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Clostridium perfringens type D is the causative agent of enterotoxemia in sheep, goats, and cattle. Although in sheep and cattle, the disease is mainly characterized by neurological clinical signs and lesions, goats with type D enterotoxemia frequently have alterations of the alimentary system. Epsilon toxin (ETX) is the main virulence factor of C. perfringens type D, although the role of ETX in intestinal lesions in goats with type D enterotoxemia has not been fully characterized. We evaluated the contribution of ETX to C. perfringens type D enteric pathogenicity using an intraduodenal challenge model in young goats, with the virulent C. perfringens type D wild-type strain CN1020; its isogenic etx null mutant; an etx-complemented strain; and sterile, non-toxic culture medium. The intestinal tract of each animal was evaluated grossly, microscopically, and immunohistochemically for activated caspase-3. Both ETX-producing strains induced extensive enterocolitis characterized by severe mucosal necrosis, apoptosis, and diffuse suppurative infiltrates. No significant gross or microscopic lesions were observed in goats inoculated with the non–ETX-containing inocula. These results confirm that ETX is essential for the production of intestinal lesions in goats with type D disease. Also, our results suggest that the intestinal pathology of type D enterotoxemia in goats is, at least in part, associated with apoptosis.
EEA Balcarce
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Morrell, Eleonora Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Navarro, Mauricio A.Universidad Austral de Chile; Chile
Fil: Garcia, Jorge P. University of California; Estados Unidos
Fil: Beingesser, Juliann. University of California; Estados Unidos
Fil: Uzal, Francisco A. University of California; Estados Unidos
Fuente
Veterinary Pathology 62 (1): 53-63 (january 2025)
Materia
Clostridium perfringens
Patología
Caprinos
Apoptosis
Enterotoxemia
Necrosis
Pathology
Goats
Enterotoxaemia
Intestinal Pathology
Clostridium perfringens type D
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26710
Acceder
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network_name_str INTA Digital (INTA)
spelling Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivativesMorrell, Eleonora LidiaNavarro, Mauricio A.Garcia, Jorge P.Beingesser, JuliannUzal, Francisco A.Clostridium perfringensPatologíaCaprinosApoptosisEnterotoxemiaNecrosisPathologyGoatsEnterotoxaemiaIntestinal PathologyClostridium perfringens type DClostridium perfringens type D is the causative agent of enterotoxemia in sheep, goats, and cattle. Although in sheep and cattle, the disease is mainly characterized by neurological clinical signs and lesions, goats with type D enterotoxemia frequently have alterations of the alimentary system. Epsilon toxin (ETX) is the main virulence factor of C. perfringens type D, although the role of ETX in intestinal lesions in goats with type D enterotoxemia has not been fully characterized. We evaluated the contribution of ETX to C. perfringens type D enteric pathogenicity using an intraduodenal challenge model in young goats, with the virulent C. perfringens type D wild-type strain CN1020; its isogenic etx null mutant; an etx-complemented strain; and sterile, non-toxic culture medium. The intestinal tract of each animal was evaluated grossly, microscopically, and immunohistochemically for activated caspase-3. Both ETX-producing strains induced extensive enterocolitis characterized by severe mucosal necrosis, apoptosis, and diffuse suppurative infiltrates. No significant gross or microscopic lesions were observed in goats inoculated with the non–ETX-containing inocula. These results confirm that ETX is essential for the production of intestinal lesions in goats with type D disease. Also, our results suggest that the intestinal pathology of type D enterotoxemia in goats is, at least in part, associated with apoptosis.EEA BalcarceFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Morrell, Eleonora Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Navarro, Mauricio A.Universidad Austral de Chile; ChileFil: Garcia, Jorge P. University of California; Estados UnidosFil: Beingesser, Juliann. University of California; Estados UnidosFil: Uzal, Francisco A. University of California; Estados UnidosSAGE Publications2026-06-19T13:56:02Z2026-06-19T13:56:02Z2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26710https://journals.sagepub.com/doi/10.1177/030098582412731220300-98581544-2217https://doi.org/10.1177/03009858241273122Veterinary Pathology 62 (1): 53-63 (january 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:22Zoai:localhost:20.500.12123/26710instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:23.516INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives
title Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives
spellingShingle Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives
Morrell, Eleonora Lidia
Clostridium perfringens
Patología
Caprinos
Apoptosis
Enterotoxemia
Necrosis
Pathology
Goats
Enterotoxaemia
Intestinal Pathology
Clostridium perfringens type D
title_short Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives
title_full Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives
title_fullStr Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives
title_full_unstemmed Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives
title_sort Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives
dc.creator.none.fl_str_mv Morrell, Eleonora Lidia
Navarro, Mauricio A.
Garcia, Jorge P.
Beingesser, Juliann
Uzal, Francisco A.
author Morrell, Eleonora Lidia
author_facet Morrell, Eleonora Lidia
Navarro, Mauricio A.
Garcia, Jorge P.
Beingesser, Juliann
Uzal, Francisco A.
author_role author
author2 Navarro, Mauricio A.
Garcia, Jorge P.
Beingesser, Juliann
Uzal, Francisco A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Clostridium perfringens
Patología
Caprinos
Apoptosis
Enterotoxemia
Necrosis
Pathology
Goats
Enterotoxaemia
Intestinal Pathology
Clostridium perfringens type D
topic Clostridium perfringens
Patología
Caprinos
Apoptosis
Enterotoxemia
Necrosis
Pathology
Goats
Enterotoxaemia
Intestinal Pathology
Clostridium perfringens type D
dc.description.none.fl_txt_mv Clostridium perfringens type D is the causative agent of enterotoxemia in sheep, goats, and cattle. Although in sheep and cattle, the disease is mainly characterized by neurological clinical signs and lesions, goats with type D enterotoxemia frequently have alterations of the alimentary system. Epsilon toxin (ETX) is the main virulence factor of C. perfringens type D, although the role of ETX in intestinal lesions in goats with type D enterotoxemia has not been fully characterized. We evaluated the contribution of ETX to C. perfringens type D enteric pathogenicity using an intraduodenal challenge model in young goats, with the virulent C. perfringens type D wild-type strain CN1020; its isogenic etx null mutant; an etx-complemented strain; and sterile, non-toxic culture medium. The intestinal tract of each animal was evaluated grossly, microscopically, and immunohistochemically for activated caspase-3. Both ETX-producing strains induced extensive enterocolitis characterized by severe mucosal necrosis, apoptosis, and diffuse suppurative infiltrates. No significant gross or microscopic lesions were observed in goats inoculated with the non–ETX-containing inocula. These results confirm that ETX is essential for the production of intestinal lesions in goats with type D disease. Also, our results suggest that the intestinal pathology of type D enterotoxemia in goats is, at least in part, associated with apoptosis.
EEA Balcarce
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Morrell, Eleonora Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Navarro, Mauricio A.Universidad Austral de Chile; Chile
Fil: Garcia, Jorge P. University of California; Estados Unidos
Fil: Beingesser, Juliann. University of California; Estados Unidos
Fil: Uzal, Francisco A. University of California; Estados Unidos
description Clostridium perfringens type D is the causative agent of enterotoxemia in sheep, goats, and cattle. Although in sheep and cattle, the disease is mainly characterized by neurological clinical signs and lesions, goats with type D enterotoxemia frequently have alterations of the alimentary system. Epsilon toxin (ETX) is the main virulence factor of C. perfringens type D, although the role of ETX in intestinal lesions in goats with type D enterotoxemia has not been fully characterized. We evaluated the contribution of ETX to C. perfringens type D enteric pathogenicity using an intraduodenal challenge model in young goats, with the virulent C. perfringens type D wild-type strain CN1020; its isogenic etx null mutant; an etx-complemented strain; and sterile, non-toxic culture medium. The intestinal tract of each animal was evaluated grossly, microscopically, and immunohistochemically for activated caspase-3. Both ETX-producing strains induced extensive enterocolitis characterized by severe mucosal necrosis, apoptosis, and diffuse suppurative infiltrates. No significant gross or microscopic lesions were observed in goats inoculated with the non–ETX-containing inocula. These results confirm that ETX is essential for the production of intestinal lesions in goats with type D disease. Also, our results suggest that the intestinal pathology of type D enterotoxemia in goats is, at least in part, associated with apoptosis.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
2026-06-19T13:56:02Z
2026-06-19T13:56:02Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26710
https://journals.sagepub.com/doi/10.1177/03009858241273122
0300-9858
1544-2217
https://doi.org/10.1177/03009858241273122
url http://hdl.handle.net/20.500.12123/26710
https://journals.sagepub.com/doi/10.1177/03009858241273122
https://doi.org/10.1177/03009858241273122
identifier_str_mv 0300-9858
1544-2217
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv Veterinary Pathology 62 (1): 53-63 (january 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.733355

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