Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditions

Autores
Marinoni, Lorena del Rosario; Gonçalves dos Santos, Iara; Tomas, Pablo Andrés; Ordoñez, Vanesa; Bustos, Dolores Angela; Zabala, Juan Marcelo
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Developing drought-tolerant forage legumes adapted to saline-sodic soils is essential to sustaining livestock production in marginal environments. Pre-breeding programs allow the identification of useful genetic variation within naturalized populations for this purpose. Aims. This study aimed to (i) compare the performance of half-sib families (HSFs) from a naturalized population of Lotus tenuis, with a commercial cultivar under drought stress, and (ii) identify key traits and promising materials for breeding. Methods. Twenty HSFs and one cultivar were evaluated under controlled conditions in a factorial design with two water regimes (field capacity and drought). Morphological, physiological and biochemical traits were measured, including aerial dry biomass, number of branches, leaf temperature, ion concentrations (Na+,K+), relative water content and oxidative stress indicators. Narrow-sense heritability and genetic correlations were estimated, and multivariate analyses were performed. Key results. Drought significantly reduced growth and altered physiological traits, with differential responses among genotypes. Some HSFs matched or exceeded the cultivar for aerial biomass production under stress. High heritability (h2 > 0.8) was observed for aerial biomass, number of branches, leaf temperature and ion concentrations under drought. Growth traits were positively correlated and negatively associated with leaf temperature and Na+ . Conclusions. Five HSFs (9, 12, 13, 16, and 20) were identified as promising materials on the basis of their performance for aerial biomass, leaf temperature, and Na+ concentration, traits identified as reliable selection criteria for drought tolerance under saline-sodic soil conditions in L.tenuis. Implications. These families provide valuable resources for breeding drought-tolerantcultivars for saline drylands, although further selection and recombination are required.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Marinoni, Lorena del Rosario. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina
Fil: Marinoni, Lorena del Rosario. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Gonçalves dos Santos, Iara. Universidade Federal de Viçosa; Brasil
Fil: Tomas, Pablo Andrés. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Ordoñez, Vanesa. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Bustos, Dolores Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Zabala, Juan Marcelo. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina
Fil: Zabala, Juan Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fuente
Crop and Pasture Science 77 (8) : CP26152. (August 2026)
Materia
Genetic Correlation
Heritability
Oxidative Stress
Phenotyping
Correlación Genética
Heredabilidad
Estrés Oxidativo
Fenotipado
Lotus tenuis
Half-sib Families
Multi-trait Selection
Narrow Leaf Trefoil
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27219
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27219
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditionsMarinoni, Lorena del RosarioGonçalves dos Santos, IaraTomas, Pablo AndrésOrdoñez, VanesaBustos, Dolores AngelaZabala, Juan MarceloGenetic CorrelationHeritabilityOxidative StressPhenotypingCorrelación GenéticaHeredabilidadEstrés OxidativoFenotipadoLotus tenuisHalf-sib FamiliesMulti-trait SelectionNarrow Leaf TrefoilDeveloping drought-tolerant forage legumes adapted to saline-sodic soils is essential to sustaining livestock production in marginal environments. Pre-breeding programs allow the identification of useful genetic variation within naturalized populations for this purpose. Aims. This study aimed to (i) compare the performance of half-sib families (HSFs) from a naturalized population of Lotus tenuis, with a commercial cultivar under drought stress, and (ii) identify key traits and promising materials for breeding. Methods. Twenty HSFs and one cultivar were evaluated under controlled conditions in a factorial design with two water regimes (field capacity and drought). Morphological, physiological and biochemical traits were measured, including aerial dry biomass, number of branches, leaf temperature, ion concentrations (Na+,K+), relative water content and oxidative stress indicators. Narrow-sense heritability and genetic correlations were estimated, and multivariate analyses were performed. Key results. Drought significantly reduced growth and altered physiological traits, with differential responses among genotypes. Some HSFs matched or exceeded the cultivar for aerial biomass production under stress. High heritability (h2 > 0.8) was observed for aerial biomass, number of branches, leaf temperature and ion concentrations under drought. Growth traits were positively correlated and negatively associated with leaf temperature and Na+ . Conclusions. Five HSFs (9, 12, 13, 16, and 20) were identified as promising materials on the basis of their performance for aerial biomass, leaf temperature, and Na+ concentration, traits identified as reliable selection criteria for drought tolerance under saline-sodic soil conditions in L.tenuis. Implications. These families provide valuable resources for breeding drought-tolerantcultivars for saline drylands, although further selection and recombination are required.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Marinoni, Lorena del Rosario. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); ArgentinaFil: Marinoni, Lorena del Rosario. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: Gonçalves dos Santos, Iara. Universidade Federal de Viçosa; BrasilFil: Tomas, Pablo Andrés. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: Ordoñez, Vanesa. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Bustos, Dolores Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Zabala, Juan Marcelo. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); ArgentinaFil: Zabala, Juan Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaCSIRO Publishing2026-07-31T10:32:30Z2026-07-31T10:32:30Z2026-07-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27219https://connectsci.au/cp/article-abstract/77/8/CP26152/275047/Pre-breeding-for-drought-tolerance-in-Lotus-tenuis1836-09471836-5795https://doi.org/10.1071/CP26152Crop and Pasture Science 77 (8) : CP26152. (August 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:36Zoai:localhost:20.500.12123/27219instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:37.419INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditions
title Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditions
spellingShingle Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditions
Marinoni, Lorena del Rosario
Genetic Correlation
Heritability
Oxidative Stress
Phenotyping
Correlación Genética
Heredabilidad
Estrés Oxidativo
Fenotipado
Lotus tenuis
Half-sib Families
Multi-trait Selection
Narrow Leaf Trefoil
title_short Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditions
title_full Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditions
title_fullStr Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditions
title_full_unstemmed Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditions
title_sort Pre-breeding for drought tolerance in Lotus tenuis under saline-sodic soil conditions
dc.creator.none.fl_str_mv Marinoni, Lorena del Rosario
Gonçalves dos Santos, Iara
Tomas, Pablo Andrés
Ordoñez, Vanesa
Bustos, Dolores Angela
Zabala, Juan Marcelo
author Marinoni, Lorena del Rosario
author_facet Marinoni, Lorena del Rosario
Gonçalves dos Santos, Iara
Tomas, Pablo Andrés
Ordoñez, Vanesa
Bustos, Dolores Angela
Zabala, Juan Marcelo
author_role author
author2 Gonçalves dos Santos, Iara
Tomas, Pablo Andrés
Ordoñez, Vanesa
Bustos, Dolores Angela
Zabala, Juan Marcelo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Genetic Correlation
Heritability
Oxidative Stress
Phenotyping
Correlación Genética
Heredabilidad
Estrés Oxidativo
Fenotipado
Lotus tenuis
Half-sib Families
Multi-trait Selection
Narrow Leaf Trefoil
topic Genetic Correlation
Heritability
Oxidative Stress
Phenotyping
Correlación Genética
Heredabilidad
Estrés Oxidativo
Fenotipado
Lotus tenuis
Half-sib Families
Multi-trait Selection
Narrow Leaf Trefoil
dc.description.none.fl_txt_mv Developing drought-tolerant forage legumes adapted to saline-sodic soils is essential to sustaining livestock production in marginal environments. Pre-breeding programs allow the identification of useful genetic variation within naturalized populations for this purpose. Aims. This study aimed to (i) compare the performance of half-sib families (HSFs) from a naturalized population of Lotus tenuis, with a commercial cultivar under drought stress, and (ii) identify key traits and promising materials for breeding. Methods. Twenty HSFs and one cultivar were evaluated under controlled conditions in a factorial design with two water regimes (field capacity and drought). Morphological, physiological and biochemical traits were measured, including aerial dry biomass, number of branches, leaf temperature, ion concentrations (Na+,K+), relative water content and oxidative stress indicators. Narrow-sense heritability and genetic correlations were estimated, and multivariate analyses were performed. Key results. Drought significantly reduced growth and altered physiological traits, with differential responses among genotypes. Some HSFs matched or exceeded the cultivar for aerial biomass production under stress. High heritability (h2 > 0.8) was observed for aerial biomass, number of branches, leaf temperature and ion concentrations under drought. Growth traits were positively correlated and negatively associated with leaf temperature and Na+ . Conclusions. Five HSFs (9, 12, 13, 16, and 20) were identified as promising materials on the basis of their performance for aerial biomass, leaf temperature, and Na+ concentration, traits identified as reliable selection criteria for drought tolerance under saline-sodic soil conditions in L.tenuis. Implications. These families provide valuable resources for breeding drought-tolerantcultivars for saline drylands, although further selection and recombination are required.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Marinoni, Lorena del Rosario. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina
Fil: Marinoni, Lorena del Rosario. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Gonçalves dos Santos, Iara. Universidade Federal de Viçosa; Brasil
Fil: Tomas, Pablo Andrés. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Ordoñez, Vanesa. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Bustos, Dolores Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Zabala, Juan Marcelo. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro Litoral); Argentina
Fil: Zabala, Juan Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
description Developing drought-tolerant forage legumes adapted to saline-sodic soils is essential to sustaining livestock production in marginal environments. Pre-breeding programs allow the identification of useful genetic variation within naturalized populations for this purpose. Aims. This study aimed to (i) compare the performance of half-sib families (HSFs) from a naturalized population of Lotus tenuis, with a commercial cultivar under drought stress, and (ii) identify key traits and promising materials for breeding. Methods. Twenty HSFs and one cultivar were evaluated under controlled conditions in a factorial design with two water regimes (field capacity and drought). Morphological, physiological and biochemical traits were measured, including aerial dry biomass, number of branches, leaf temperature, ion concentrations (Na+,K+), relative water content and oxidative stress indicators. Narrow-sense heritability and genetic correlations were estimated, and multivariate analyses were performed. Key results. Drought significantly reduced growth and altered physiological traits, with differential responses among genotypes. Some HSFs matched or exceeded the cultivar for aerial biomass production under stress. High heritability (h2 > 0.8) was observed for aerial biomass, number of branches, leaf temperature and ion concentrations under drought. Growth traits were positively correlated and negatively associated with leaf temperature and Na+ . Conclusions. Five HSFs (9, 12, 13, 16, and 20) were identified as promising materials on the basis of their performance for aerial biomass, leaf temperature, and Na+ concentration, traits identified as reliable selection criteria for drought tolerance under saline-sodic soil conditions in L.tenuis. Implications. These families provide valuable resources for breeding drought-tolerantcultivars for saline drylands, although further selection and recombination are required.
publishDate 2026
dc.date.none.fl_str_mv 2026-07-31T10:32:30Z
2026-07-31T10:32:30Z
2026-07-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27219
https://connectsci.au/cp/article-abstract/77/8/CP26152/275047/Pre-breeding-for-drought-tolerance-in-Lotus-tenuis
1836-0947
1836-5795
https://doi.org/10.1071/CP26152
url http://hdl.handle.net/20.500.12123/27219
https://connectsci.au/cp/article-abstract/77/8/CP26152/275047/Pre-breeding-for-drought-tolerance-in-Lotus-tenuis
https://doi.org/10.1071/CP26152
identifier_str_mv 1836-0947
1836-5795
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv CSIRO Publishing
publisher.none.fl_str_mv CSIRO Publishing
dc.source.none.fl_str_mv Crop and Pasture Science 77 (8) : CP26152. (August 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.754232

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