How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulc...

Autores
Guillen, Noelia Belén; Setten, Lorena; Favret, Eduardo Alfredo
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One way to develop non-stick surfaces for soil tillage tools is to study the cuticular topography of digging beetles. In this work, we compared the micro-relief characteristics of the cuticular surface of the pronotum of female and male beetles of the genus Sulcophanaeus (Coleoptera, Scarabaeidae) to find those common features that can be used to design a non-stick topographic pattern. Translucent replicas of the pronotum of each specimen were prepared and were observed with an optical microscopy. Sequential images of one half of the pronotum were taken and measured using ImageJ software and analyzed with R statistical software. Six segments were identified on the pronotum of males and females: lateral (S1, S3 and S5) and central (S2, S4 and S6). All specimens examined had dimples on the pronotum surface. The variables analyzed were a) dimple area [µm2]; b) density, as total dimple area/total surface area; c) distance between dimples [µm] and d) eccentricity (minor dimple diameter/major dimple diameter). Considering these variables Ward’s hierarchical clustering method was performed. The classification was done using Euclidean distance. Clear differences between the lateral and central sectors are observed in all species. Central sectors S2 and S6 in both males and females show the greatest variability. Sectors 1, 3, 5, and 4 have common characteristics and could be used to create a non-stick design. The next step is to study and characterize the cuticle of telecoprid dung beetles.
Instituto de Suelos
Fil: Guillén, Noelia Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fil: Guillén, Noelia Belén. Consejo Nacional de Investigaciones Científica y Técnicas; Argentina
Fil: Setten, Lorena María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fil: Favret, Eduardo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fil: Favret, Eduardo Alfredo. Consejo Nacional de Investigaciones Científica y Técnicas; Argentina
Fuente
Microscopy and Microanalysis 32 (7) : 538-541(July 2026)
Materia
Dung Beetles
Tillage Equipment
Soil Management Equipment
Escarabajo del Estiércol
Maquinaria de Labranza
Maquinaria para Manejo del Suelo
Sulcophanaeus
Cuticular Topography
Non-stick Topographic Pattern
Topografía Cuticular
Patrón Topográfico Antiadherente
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/28010
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oai_identifier_str oai:localhost:20.500.12123/28010
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelasGuillen, Noelia BelénSetten, LorenaFavret, Eduardo AlfredoDung BeetlesTillage EquipmentSoil Management EquipmentEscarabajo del EstiércolMaquinaria de LabranzaMaquinaria para Manejo del SueloSulcophanaeusCuticular TopographyNon-stick Topographic PatternTopografía CuticularPatrón Topográfico AntiadherenteOne way to develop non-stick surfaces for soil tillage tools is to study the cuticular topography of digging beetles. In this work, we compared the micro-relief characteristics of the cuticular surface of the pronotum of female and male beetles of the genus Sulcophanaeus (Coleoptera, Scarabaeidae) to find those common features that can be used to design a non-stick topographic pattern. Translucent replicas of the pronotum of each specimen were prepared and were observed with an optical microscopy. Sequential images of one half of the pronotum were taken and measured using ImageJ software and analyzed with R statistical software. Six segments were identified on the pronotum of males and females: lateral (S1, S3 and S5) and central (S2, S4 and S6). All specimens examined had dimples on the pronotum surface. The variables analyzed were a) dimple area [µm2]; b) density, as total dimple area/total surface area; c) distance between dimples [µm] and d) eccentricity (minor dimple diameter/major dimple diameter). Considering these variables Ward’s hierarchical clustering method was performed. The classification was done using Euclidean distance. Clear differences between the lateral and central sectors are observed in all species. Central sectors S2 and S6 in both males and females show the greatest variability. Sectors 1, 3, 5, and 4 have common characteristics and could be used to create a non-stick design. The next step is to study and characterize the cuticle of telecoprid dung beetles.Instituto de SuelosFil: Guillén, Noelia Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; ArgentinaFil: Guillén, Noelia Belén. Consejo Nacional de Investigaciones Científica y Técnicas; ArgentinaFil: Setten, Lorena María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; ArgentinaFil: Favret, Eduardo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; ArgentinaFil: Favret, Eduardo Alfredo. Consejo Nacional de Investigaciones Científica y Técnicas; ArgentinaOxford University Press2026-10-06T10:06:59Z2026-10-06T10:06:59Z2026-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/28010https://academic.oup.com/mam/article/32/Supplement_1/ozag053.257/8742598Guillén, N.B., Setten, L.M., and, Favret, E.A. (2026, July). How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas. Microscopy and Microanalysis, 32 (7), 538–541.1431-92761435-8115https://doi.org/10.1093/mam/ozag053.257Microscopy and Microanalysis 32 (7) : 538-541(July 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-11-05T11:51:39Zoai:localhost:20.500.12123/28010instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-11-05 11:51:40.179INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas
title How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas
spellingShingle How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas
Guillen, Noelia Belén
Dung Beetles
Tillage Equipment
Soil Management Equipment
Escarabajo del Estiércol
Maquinaria de Labranza
Maquinaria para Manejo del Suelo
Sulcophanaeus
Cuticular Topography
Non-stick Topographic Pattern
Topografía Cuticular
Patrón Topográfico Antiadherente
title_short How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas
title_full How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas
title_fullStr How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas
title_full_unstemmed How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas
title_sort How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas
dc.creator.none.fl_str_mv Guillen, Noelia Belén
Setten, Lorena
Favret, Eduardo Alfredo
author Guillen, Noelia Belén
author_facet Guillen, Noelia Belén
Setten, Lorena
Favret, Eduardo Alfredo
author_role author
author2 Setten, Lorena
Favret, Eduardo Alfredo
author2_role author
author
dc.subject.none.fl_str_mv Dung Beetles
Tillage Equipment
Soil Management Equipment
Escarabajo del Estiércol
Maquinaria de Labranza
Maquinaria para Manejo del Suelo
Sulcophanaeus
Cuticular Topography
Non-stick Topographic Pattern
Topografía Cuticular
Patrón Topográfico Antiadherente
topic Dung Beetles
Tillage Equipment
Soil Management Equipment
Escarabajo del Estiércol
Maquinaria de Labranza
Maquinaria para Manejo del Suelo
Sulcophanaeus
Cuticular Topography
Non-stick Topographic Pattern
Topografía Cuticular
Patrón Topográfico Antiadherente
dc.description.none.fl_txt_mv One way to develop non-stick surfaces for soil tillage tools is to study the cuticular topography of digging beetles. In this work, we compared the micro-relief characteristics of the cuticular surface of the pronotum of female and male beetles of the genus Sulcophanaeus (Coleoptera, Scarabaeidae) to find those common features that can be used to design a non-stick topographic pattern. Translucent replicas of the pronotum of each specimen were prepared and were observed with an optical microscopy. Sequential images of one half of the pronotum were taken and measured using ImageJ software and analyzed with R statistical software. Six segments were identified on the pronotum of males and females: lateral (S1, S3 and S5) and central (S2, S4 and S6). All specimens examined had dimples on the pronotum surface. The variables analyzed were a) dimple area [µm2]; b) density, as total dimple area/total surface area; c) distance between dimples [µm] and d) eccentricity (minor dimple diameter/major dimple diameter). Considering these variables Ward’s hierarchical clustering method was performed. The classification was done using Euclidean distance. Clear differences between the lateral and central sectors are observed in all species. Central sectors S2 and S6 in both males and females show the greatest variability. Sectors 1, 3, 5, and 4 have common characteristics and could be used to create a non-stick design. The next step is to study and characterize the cuticle of telecoprid dung beetles.
Instituto de Suelos
Fil: Guillén, Noelia Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fil: Guillén, Noelia Belén. Consejo Nacional de Investigaciones Científica y Técnicas; Argentina
Fil: Setten, Lorena María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fil: Favret, Eduardo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fil: Favret, Eduardo Alfredo. Consejo Nacional de Investigaciones Científica y Técnicas; Argentina
description One way to develop non-stick surfaces for soil tillage tools is to study the cuticular topography of digging beetles. In this work, we compared the micro-relief characteristics of the cuticular surface of the pronotum of female and male beetles of the genus Sulcophanaeus (Coleoptera, Scarabaeidae) to find those common features that can be used to design a non-stick topographic pattern. Translucent replicas of the pronotum of each specimen were prepared and were observed with an optical microscopy. Sequential images of one half of the pronotum were taken and measured using ImageJ software and analyzed with R statistical software. Six segments were identified on the pronotum of males and females: lateral (S1, S3 and S5) and central (S2, S4 and S6). All specimens examined had dimples on the pronotum surface. The variables analyzed were a) dimple area [µm2]; b) density, as total dimple area/total surface area; c) distance between dimples [µm] and d) eccentricity (minor dimple diameter/major dimple diameter). Considering these variables Ward’s hierarchical clustering method was performed. The classification was done using Euclidean distance. Clear differences between the lateral and central sectors are observed in all species. Central sectors S2 and S6 in both males and females show the greatest variability. Sectors 1, 3, 5, and 4 have common characteristics and could be used to create a non-stick design. The next step is to study and characterize the cuticle of telecoprid dung beetles.
publishDate 2026
dc.date.none.fl_str_mv 2026-10-06T10:06:59Z
2026-10-06T10:06:59Z
2026-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/28010
https://academic.oup.com/mam/article/32/Supplement_1/ozag053.257/8742598
Guillén, N.B., Setten, L.M., and, Favret, E.A. (2026, July). How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas. Microscopy and Microanalysis, 32 (7), 538–541.
1431-9276
1435-8115
https://doi.org/10.1093/mam/ozag053.257
url http://hdl.handle.net/20.500.12123/28010
https://academic.oup.com/mam/article/32/Supplement_1/ozag053.257/8742598
https://doi.org/10.1093/mam/ozag053.257
identifier_str_mv Guillén, N.B., Setten, L.M., and, Favret, E.A. (2026, July). How to Improve Soil Anti-Adhesion by Studying the Micro Relief of the Cuticle Surface of Digging Beetles: Comparative study of the Pronotum Topography of Sulcophanaeus batesi, Sulcophanaeus imperator and Sulcophanaeus menelas. Microscopy and Microanalysis, 32 (7), 538–541.
1431-9276
1435-8115
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Microscopy and Microanalysis 32 (7) : 538-541(July 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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