Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production

Autores
Ulla, Sofía Belén; Jacquat, Andrés Gustavo; Cañizares, María Carmen; Theumer, Martín Gustavo; López-Sesé, Ana Isabel; García Pedrajas, María Dolores; Dambolena, José Sebastián
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycoviruses represent promising tools for the biological control of phytopathogenic fungi. However, their application is often constrained by host specificity and low natural prevalence in certain pathogens. Fusarium verticillioides, a major maize pathogen and fumonisin B1 (FB1) producer, harbors very few known mycoviruses, a circumstance that limits the development of virus-based control strategies. In this study, we explored a virocontrol approach for F. verticillioides based on the use of a heterologous mycovirus, Cryphonectria hypovirus 1 (CHV1). For the artificial transfection of F. verticillioides with CHV1, we used an infectious cDNA clone of the virus and protoplast-mediated transformation. Through RT-PCR and double-stranded RNA (dsRNA) purification by chromatography on cellulose, it was confirmed that the CHV1 infectious cDNA clone is stably integrated into the fungal genome, giving rise to autonomous virus replication in the cytoplasm of F. verticillioides cells. Characterization of four independent transformants harboring CHV1 showed a significant reduction in FB1 production compared to the parental uninfected strain and only moderate alterations in vegetative growth. The CHV1-associated reduction in FB1 was confirmed in maize grown under both greenhouse and field conditions. Our findings provide evidence that heterologous mycoviruses can modulate mycotoxin production in F. verticillioides, thus expanding the pool of viral species that can be explored as potential biocontrol agents in this pathogen. Further work will be required to elucidate the underlying mechanisms of CHV1-associated downregulation of FB1 production in F. verticillioides and assess its potential application in virocontrol strategies.
Instituto de Patología Vegetal
Fil: Ulla, Sofía Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina
Fil: Ulla, Sofía Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Ulla, Sofía Belén. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Jacquat, Andrés Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Jacquat, Andrés Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cañizares, María Carmen. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: Theumer, Martín Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
Fil: Theumer, Martín Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina
Fil: López-Sesé, Ana Isabel. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: García Pedrajas, María Dolores. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM - UMA - CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina
Fil: Dambolena, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fuente
Viruses 18 (9) : 971. (September 2026)
Materia
Fusarium
Fumonisins
Mycotoxins
Immunization
Cryphonectria
Fumonisina
Micotoxinas
Inmunización
Fusarium verticillioides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/28082
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network_name_str INTA Digital (INTA)
spelling Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin ProductionUlla, Sofía BelénJacquat, Andrés GustavoCañizares, María CarmenTheumer, Martín GustavoLópez-Sesé, Ana IsabelGarcía Pedrajas, María DoloresDambolena, José SebastiánFusariumFumonisinsMycotoxinsImmunizationCryphonectriaFumonisinaMicotoxinasInmunizaciónFusarium verticillioidesMycoviruses represent promising tools for the biological control of phytopathogenic fungi. However, their application is often constrained by host specificity and low natural prevalence in certain pathogens. Fusarium verticillioides, a major maize pathogen and fumonisin B1 (FB1) producer, harbors very few known mycoviruses, a circumstance that limits the development of virus-based control strategies. In this study, we explored a virocontrol approach for F. verticillioides based on the use of a heterologous mycovirus, Cryphonectria hypovirus 1 (CHV1). For the artificial transfection of F. verticillioides with CHV1, we used an infectious cDNA clone of the virus and protoplast-mediated transformation. Through RT-PCR and double-stranded RNA (dsRNA) purification by chromatography on cellulose, it was confirmed that the CHV1 infectious cDNA clone is stably integrated into the fungal genome, giving rise to autonomous virus replication in the cytoplasm of F. verticillioides cells. Characterization of four independent transformants harboring CHV1 showed a significant reduction in FB1 production compared to the parental uninfected strain and only moderate alterations in vegetative growth. The CHV1-associated reduction in FB1 was confirmed in maize grown under both greenhouse and field conditions. Our findings provide evidence that heterologous mycoviruses can modulate mycotoxin production in F. verticillioides, thus expanding the pool of viral species that can be explored as potential biocontrol agents in this pathogen. Further work will be required to elucidate the underlying mechanisms of CHV1-associated downregulation of FB1 production in F. verticillioides and assess its potential application in virocontrol strategies.Instituto de Patología VegetalFil: Ulla, Sofía Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; ArgentinaFil: Ulla, Sofía Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal (IMBIV); ArgentinaFil: Ulla, Sofía Belén. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal (IMBIV); ArgentinaFil: Jacquat, Andrés Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Jacquat, Andrés Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Cañizares, María Carmen. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; EspañaFil: Theumer, Martín Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; ArgentinaFil: Theumer, Martín Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); ArgentinaFil: López-Sesé, Ana Isabel. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; EspañaFil: García Pedrajas, María Dolores. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM - UMA - CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; EspañaFil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; ArgentinaFil: Dambolena, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal (IMBIV); ArgentinaFil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal (IMBIV); ArgentinaMDPI2026-10-13T12:33:52Z2026-10-13T12:33:52Z2026-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/28082https://www.mdpi.com/1999-4915/18/9/9711999-4915https://doi.org/10.3390/v18090971Viruses 18 (9) : 971. (September 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-11-05T11:51:40Zoai:localhost:20.500.12123/28082instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-11-05 11:51:40.893INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production
title Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production
spellingShingle Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production
Ulla, Sofía Belén
Fusarium
Fumonisins
Mycotoxins
Immunization
Cryphonectria
Fumonisina
Micotoxinas
Inmunización
Fusarium verticillioides
title_short Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production
title_full Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production
title_fullStr Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production
title_full_unstemmed Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production
title_sort Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production
dc.creator.none.fl_str_mv Ulla, Sofía Belén
Jacquat, Andrés Gustavo
Cañizares, María Carmen
Theumer, Martín Gustavo
López-Sesé, Ana Isabel
García Pedrajas, María Dolores
Dambolena, José Sebastián
author Ulla, Sofía Belén
author_facet Ulla, Sofía Belén
Jacquat, Andrés Gustavo
Cañizares, María Carmen
Theumer, Martín Gustavo
López-Sesé, Ana Isabel
García Pedrajas, María Dolores
Dambolena, José Sebastián
author_role author
author2 Jacquat, Andrés Gustavo
Cañizares, María Carmen
Theumer, Martín Gustavo
López-Sesé, Ana Isabel
García Pedrajas, María Dolores
Dambolena, José Sebastián
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fusarium
Fumonisins
Mycotoxins
Immunization
Cryphonectria
Fumonisina
Micotoxinas
Inmunización
Fusarium verticillioides
topic Fusarium
Fumonisins
Mycotoxins
Immunization
Cryphonectria
Fumonisina
Micotoxinas
Inmunización
Fusarium verticillioides
dc.description.none.fl_txt_mv Mycoviruses represent promising tools for the biological control of phytopathogenic fungi. However, their application is often constrained by host specificity and low natural prevalence in certain pathogens. Fusarium verticillioides, a major maize pathogen and fumonisin B1 (FB1) producer, harbors very few known mycoviruses, a circumstance that limits the development of virus-based control strategies. In this study, we explored a virocontrol approach for F. verticillioides based on the use of a heterologous mycovirus, Cryphonectria hypovirus 1 (CHV1). For the artificial transfection of F. verticillioides with CHV1, we used an infectious cDNA clone of the virus and protoplast-mediated transformation. Through RT-PCR and double-stranded RNA (dsRNA) purification by chromatography on cellulose, it was confirmed that the CHV1 infectious cDNA clone is stably integrated into the fungal genome, giving rise to autonomous virus replication in the cytoplasm of F. verticillioides cells. Characterization of four independent transformants harboring CHV1 showed a significant reduction in FB1 production compared to the parental uninfected strain and only moderate alterations in vegetative growth. The CHV1-associated reduction in FB1 was confirmed in maize grown under both greenhouse and field conditions. Our findings provide evidence that heterologous mycoviruses can modulate mycotoxin production in F. verticillioides, thus expanding the pool of viral species that can be explored as potential biocontrol agents in this pathogen. Further work will be required to elucidate the underlying mechanisms of CHV1-associated downregulation of FB1 production in F. verticillioides and assess its potential application in virocontrol strategies.
Instituto de Patología Vegetal
Fil: Ulla, Sofía Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina
Fil: Ulla, Sofía Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Ulla, Sofía Belén. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Jacquat, Andrés Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Jacquat, Andrés Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cañizares, María Carmen. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: Theumer, Martín Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
Fil: Theumer, Martín Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina
Fil: López-Sesé, Ana Isabel. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: García Pedrajas, María Dolores. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM - UMA - CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina
Fil: Dambolena, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
description Mycoviruses represent promising tools for the biological control of phytopathogenic fungi. However, their application is often constrained by host specificity and low natural prevalence in certain pathogens. Fusarium verticillioides, a major maize pathogen and fumonisin B1 (FB1) producer, harbors very few known mycoviruses, a circumstance that limits the development of virus-based control strategies. In this study, we explored a virocontrol approach for F. verticillioides based on the use of a heterologous mycovirus, Cryphonectria hypovirus 1 (CHV1). For the artificial transfection of F. verticillioides with CHV1, we used an infectious cDNA clone of the virus and protoplast-mediated transformation. Through RT-PCR and double-stranded RNA (dsRNA) purification by chromatography on cellulose, it was confirmed that the CHV1 infectious cDNA clone is stably integrated into the fungal genome, giving rise to autonomous virus replication in the cytoplasm of F. verticillioides cells. Characterization of four independent transformants harboring CHV1 showed a significant reduction in FB1 production compared to the parental uninfected strain and only moderate alterations in vegetative growth. The CHV1-associated reduction in FB1 was confirmed in maize grown under both greenhouse and field conditions. Our findings provide evidence that heterologous mycoviruses can modulate mycotoxin production in F. verticillioides, thus expanding the pool of viral species that can be explored as potential biocontrol agents in this pathogen. Further work will be required to elucidate the underlying mechanisms of CHV1-associated downregulation of FB1 production in F. verticillioides and assess its potential application in virocontrol strategies.
publishDate 2026
dc.date.none.fl_str_mv 2026-10-13T12:33:52Z
2026-10-13T12:33:52Z
2026-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/28082
https://www.mdpi.com/1999-4915/18/9/971
1999-4915
https://doi.org/10.3390/v18090971
url http://hdl.handle.net/20.500.12123/28082
https://www.mdpi.com/1999-4915/18/9/971
https://doi.org/10.3390/v18090971
identifier_str_mv 1999-4915
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Viruses 18 (9) : 971. (September 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
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repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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