Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae

Autores
Aronzon, Carolina Mariel; Peluso, Julieta; Beily, María Eugenia; Bres, Patricia Alina
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of anaerobic digestates from livestock manure as biofertilizers is a sustainable practice within the framework of the circular economy. However, their potential toxicity to aquatic ecosystems is insufficiently understood. This study assesses the toxicity of three liquid digestates (LDs) derived from pig (DP), dairy (DD), and cattle (DC) manure by means of standardised bioassays with Rhinella arenarum larvae. Physicochemical and microbiological characterisation was conducted and compared with regulatory standards. Biomarkers of oxidative stress (catalase and glutathione-S-transferase activities, and glutathione content), anticholinesterasic activity (acetylcholinesterase and butyrylcholinesterase activities), and genotoxicity (micronucleus frequency) were also performed. The LDs exhibited high levels of organic matter, carbon, and nutrients, confirming their potential as agricultural amendments. Elevated concentrations of ammonium, metals (Cu and Zn), and electrical conductivity were also detected. The DP (LC50-504 h = 0.338%) and DC (LC50-504 h = 0.347%) showed high chronic toxicity, whereas DD (LC50-504 h = 1.18%) was significantly less toxic. All LDs altered oxidative stress biomarkers, induced anticholinesterasic effects, and caused genotoxicity at sublethal concentrations. These findings highlight the importance of amphibian bioassays in the ecotoxicological evaluation of anaerobic digestates prior to their environmental application.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Aronzon, Carolina Mariel. Universidad Nacional de San Martín. Escuela de Hábitat y Sostenibilidad. Instituto de Investigación e Ingeniería Ambiental (IIIA); Argentina
Fil: Aronzon, Carolina Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación e Ingeniería Ambiental (IIIA); Argentina
Fil: Peluso, Julieta. Universidad Nacional de San Martín. Escuela de Hábitat y Sostenibilidad. Instituto de Investigación e Ingeniería Ambiental (IIIA); Argentina
Fil: Peluso, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación e Ingeniería Ambiental (IIIA); Argentina
Fil: Beily, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Bres, Patricia Alina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fuente
Chemistry and Ecology 42 (7) : 838-859. (July 2026)
Materia
Biodigesters
Biogás
Toxicology
Anaerobic Digesters
Livestock Manure
Toxicología
Biodigestor
Digestor Anaeróbico
Abono Animal
biological fertilizer
Rhinella arenarum
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27879
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27879
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network_name_str INTA Digital (INTA)
spelling Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvaeAronzon, Carolina MarielPeluso, JulietaBeily, María EugeniaBres, Patricia AlinaBiodigestersBiogásToxicologyAnaerobic DigestersLivestock ManureToxicologíaBiodigestorDigestor AnaeróbicoAbono Animalbiological fertilizerRhinella arenarumThe use of anaerobic digestates from livestock manure as biofertilizers is a sustainable practice within the framework of the circular economy. However, their potential toxicity to aquatic ecosystems is insufficiently understood. This study assesses the toxicity of three liquid digestates (LDs) derived from pig (DP), dairy (DD), and cattle (DC) manure by means of standardised bioassays with Rhinella arenarum larvae. Physicochemical and microbiological characterisation was conducted and compared with regulatory standards. Biomarkers of oxidative stress (catalase and glutathione-S-transferase activities, and glutathione content), anticholinesterasic activity (acetylcholinesterase and butyrylcholinesterase activities), and genotoxicity (micronucleus frequency) were also performed. The LDs exhibited high levels of organic matter, carbon, and nutrients, confirming their potential as agricultural amendments. Elevated concentrations of ammonium, metals (Cu and Zn), and electrical conductivity were also detected. The DP (LC50-504 h = 0.338%) and DC (LC50-504 h = 0.347%) showed high chronic toxicity, whereas DD (LC50-504 h = 1.18%) was significantly less toxic. All LDs altered oxidative stress biomarkers, induced anticholinesterasic effects, and caused genotoxicity at sublethal concentrations. These findings highlight the importance of amphibian bioassays in the ecotoxicological evaluation of anaerobic digestates prior to their environmental application.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Aronzon, Carolina Mariel. Universidad Nacional de San Martín. Escuela de Hábitat y Sostenibilidad. Instituto de Investigación e Ingeniería Ambiental (IIIA); ArgentinaFil: Aronzon, Carolina Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación e Ingeniería Ambiental (IIIA); ArgentinaFil: Peluso, Julieta. Universidad Nacional de San Martín. Escuela de Hábitat y Sostenibilidad. Instituto de Investigación e Ingeniería Ambiental (IIIA); ArgentinaFil: Peluso, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación e Ingeniería Ambiental (IIIA); ArgentinaFil: Beily, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Bres, Patricia Alina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaTaylor & Francis2026-09-22T10:50:52Z2026-09-22T10:50:52Z2026-07-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27879https://www.tandfonline.com/doi/full/10.1080/02757540.2026.27019270275-75401029-0370https://doi.org/10.1080/02757540.2026.2701927Chemistry and Ecology 42 (7) : 838-859. (July 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:52Zoai:localhost:20.500.12123/27879instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:52.586INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae
title Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae
spellingShingle Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae
Aronzon, Carolina Mariel
Biodigesters
Biogás
Toxicology
Anaerobic Digesters
Livestock Manure
Toxicología
Biodigestor
Digestor Anaeróbico
Abono Animal
biological fertilizer
Rhinella arenarum
title_short Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae
title_full Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae
title_fullStr Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae
title_full_unstemmed Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae
title_sort Comprehensive toxicity assessment of anaerobic digestates from livestock manure in Rhinella arenarum larvae
dc.creator.none.fl_str_mv Aronzon, Carolina Mariel
Peluso, Julieta
Beily, María Eugenia
Bres, Patricia Alina
author Aronzon, Carolina Mariel
author_facet Aronzon, Carolina Mariel
Peluso, Julieta
Beily, María Eugenia
Bres, Patricia Alina
author_role author
author2 Peluso, Julieta
Beily, María Eugenia
Bres, Patricia Alina
author2_role author
author
author
dc.subject.none.fl_str_mv Biodigesters
Biogás
Toxicology
Anaerobic Digesters
Livestock Manure
Toxicología
Biodigestor
Digestor Anaeróbico
Abono Animal
biological fertilizer
Rhinella arenarum
topic Biodigesters
Biogás
Toxicology
Anaerobic Digesters
Livestock Manure
Toxicología
Biodigestor
Digestor Anaeróbico
Abono Animal
biological fertilizer
Rhinella arenarum
dc.description.none.fl_txt_mv The use of anaerobic digestates from livestock manure as biofertilizers is a sustainable practice within the framework of the circular economy. However, their potential toxicity to aquatic ecosystems is insufficiently understood. This study assesses the toxicity of three liquid digestates (LDs) derived from pig (DP), dairy (DD), and cattle (DC) manure by means of standardised bioassays with Rhinella arenarum larvae. Physicochemical and microbiological characterisation was conducted and compared with regulatory standards. Biomarkers of oxidative stress (catalase and glutathione-S-transferase activities, and glutathione content), anticholinesterasic activity (acetylcholinesterase and butyrylcholinesterase activities), and genotoxicity (micronucleus frequency) were also performed. The LDs exhibited high levels of organic matter, carbon, and nutrients, confirming their potential as agricultural amendments. Elevated concentrations of ammonium, metals (Cu and Zn), and electrical conductivity were also detected. The DP (LC50-504 h = 0.338%) and DC (LC50-504 h = 0.347%) showed high chronic toxicity, whereas DD (LC50-504 h = 1.18%) was significantly less toxic. All LDs altered oxidative stress biomarkers, induced anticholinesterasic effects, and caused genotoxicity at sublethal concentrations. These findings highlight the importance of amphibian bioassays in the ecotoxicological evaluation of anaerobic digestates prior to their environmental application.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Aronzon, Carolina Mariel. Universidad Nacional de San Martín. Escuela de Hábitat y Sostenibilidad. Instituto de Investigación e Ingeniería Ambiental (IIIA); Argentina
Fil: Aronzon, Carolina Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación e Ingeniería Ambiental (IIIA); Argentina
Fil: Peluso, Julieta. Universidad Nacional de San Martín. Escuela de Hábitat y Sostenibilidad. Instituto de Investigación e Ingeniería Ambiental (IIIA); Argentina
Fil: Peluso, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación e Ingeniería Ambiental (IIIA); Argentina
Fil: Beily, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Bres, Patricia Alina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
description The use of anaerobic digestates from livestock manure as biofertilizers is a sustainable practice within the framework of the circular economy. However, their potential toxicity to aquatic ecosystems is insufficiently understood. This study assesses the toxicity of three liquid digestates (LDs) derived from pig (DP), dairy (DD), and cattle (DC) manure by means of standardised bioassays with Rhinella arenarum larvae. Physicochemical and microbiological characterisation was conducted and compared with regulatory standards. Biomarkers of oxidative stress (catalase and glutathione-S-transferase activities, and glutathione content), anticholinesterasic activity (acetylcholinesterase and butyrylcholinesterase activities), and genotoxicity (micronucleus frequency) were also performed. The LDs exhibited high levels of organic matter, carbon, and nutrients, confirming their potential as agricultural amendments. Elevated concentrations of ammonium, metals (Cu and Zn), and electrical conductivity were also detected. The DP (LC50-504 h = 0.338%) and DC (LC50-504 h = 0.347%) showed high chronic toxicity, whereas DD (LC50-504 h = 1.18%) was significantly less toxic. All LDs altered oxidative stress biomarkers, induced anticholinesterasic effects, and caused genotoxicity at sublethal concentrations. These findings highlight the importance of amphibian bioassays in the ecotoxicological evaluation of anaerobic digestates prior to their environmental application.
publishDate 2026
dc.date.none.fl_str_mv 2026-09-22T10:50:52Z
2026-09-22T10:50:52Z
2026-07-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27879
https://www.tandfonline.com/doi/full/10.1080/02757540.2026.2701927
0275-7540
1029-0370
https://doi.org/10.1080/02757540.2026.2701927
url http://hdl.handle.net/20.500.12123/27879
https://www.tandfonline.com/doi/full/10.1080/02757540.2026.2701927
https://doi.org/10.1080/02757540.2026.2701927
identifier_str_mv 0275-7540
1029-0370
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv Chemistry and Ecology 42 (7) : 838-859. (July 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.265058

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