Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen Fertilization

Autores
Oyharcabal, Estefanía; Covacevich, Fernanda; Bain, Ingrid; Acuña, Claudina Soledad; Berone, German Dario
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study assessed the effects of nitrogen (N) fertilization source on forage yield, nutritional status, and the activity/abundance of autochthonous root symbionts in fescue–lotus mixtures. Specifically, the contribution of arbuscular mycorrhizal fungi (AMF) and N-fixing rhizobia (NFR) was assessed under manure (M) and urea (U) fertilization. The aim was to determine whether autochthonous root symbiont populations enhance pasture productivity and nutritional status, mainly under organic fertilization. A nine-month pot experiment was conducted growing fescue–lotus mixtures under two soil conditions: A-soil, with autochthonous root symbiont populations; and R-soil, in which root symbiont populations (AMF and NFR) were reduced via formaldehyde application + UV treatment. Both soil conditions received either no fertilizer (Control) or equal N doses (120 kg N ha− 1 y− 1) supplied as cattle dry manure or urea. Fertilization with M resulted in root symbionts activity/abundance similar to the Control. Fertilization with U reduced root mycorrhizal colonization and nodulation. Plant growth was significantly higher in A-soil than in R-soil, with the legume component showing positive response. Under R-soil conditions, U fertilization resulted in the highest pasture growth, favoring grasses at the expense of legumes. Overall, M appears to be a more suitable fertilizer than U, as it better preserves root symbionts and may also contribute to increased productivity in the mixture. These findings suggest that M may be less disruptive to the studied root symbionts (AMF and NFR) than U under these conditions. These findings could have implications for the development of management strategies designed to preserve potentially beneficial root symbionts in pasture-based livestock systems.
EEA Chubut
Fil: Oyharcabal, Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Oyharcabal, Estefanía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; Argentina
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Bain, Ingrid. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; Argentina
Fil: Acuña, Claudina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; Argentina
Fil: Berone, German Dario. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Berone, German Dario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fuente
Journal of Soil Science and Plant Nutrition : 1-12. (Published: 10 June 2026)
Materia
Festuca arundinacea
Lotus
Fertilizer Application
Symbionts
Nitrogen Fertilizers
Arbuscular Mycorrhiza
Rhizobiaceae
Organic Fertilizers
Aplicación de Abonos
Simbiontico
Abonos Nitrogenados
Micorriza Arbuscular
Abonos Orgánicos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26688
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oai_identifier_str oai:localhost:20.500.12123/26688
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen FertilizationOyharcabal, EstefaníaCovacevich, FernandaBain, IngridAcuña, Claudina SoledadBerone, German DarioFestuca arundinaceaLotusFertilizer ApplicationSymbiontsNitrogen FertilizersArbuscular MycorrhizaRhizobiaceaeOrganic FertilizersAplicación de AbonosSimbionticoAbonos NitrogenadosMicorriza ArbuscularAbonos OrgánicosThis study assessed the effects of nitrogen (N) fertilization source on forage yield, nutritional status, and the activity/abundance of autochthonous root symbionts in fescue–lotus mixtures. Specifically, the contribution of arbuscular mycorrhizal fungi (AMF) and N-fixing rhizobia (NFR) was assessed under manure (M) and urea (U) fertilization. The aim was to determine whether autochthonous root symbiont populations enhance pasture productivity and nutritional status, mainly under organic fertilization. A nine-month pot experiment was conducted growing fescue–lotus mixtures under two soil conditions: A-soil, with autochthonous root symbiont populations; and R-soil, in which root symbiont populations (AMF and NFR) were reduced via formaldehyde application + UV treatment. Both soil conditions received either no fertilizer (Control) or equal N doses (120 kg N ha− 1 y− 1) supplied as cattle dry manure or urea. Fertilization with M resulted in root symbionts activity/abundance similar to the Control. Fertilization with U reduced root mycorrhizal colonization and nodulation. Plant growth was significantly higher in A-soil than in R-soil, with the legume component showing positive response. Under R-soil conditions, U fertilization resulted in the highest pasture growth, favoring grasses at the expense of legumes. Overall, M appears to be a more suitable fertilizer than U, as it better preserves root symbionts and may also contribute to increased productivity in the mixture. These findings suggest that M may be less disruptive to the studied root symbionts (AMF and NFR) than U under these conditions. These findings could have implications for the development of management strategies designed to preserve potentially beneficial root symbionts in pasture-based livestock systems.EEA ChubutFil: Oyharcabal, Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Oyharcabal, Estefanía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; ArgentinaFil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Bain, Ingrid. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; ArgentinaFil: Acuña, Claudina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; ArgentinaFil: Berone, German Dario. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Berone, German Dario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaSpringer2026-06-18T12:43:00Z2026-06-18T12:43:00Z2026-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26688https://link.springer.com/article/10.1007/s42729-026-03367-60718-95080718-9516https://doi.org/10.1007/s42729-026-03367-6Journal of Soil Science and Plant Nutrition : 1-12. (Published: 10 June 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E1-I007-001, Incremento sostenible de la producción y utilización de pasturas y forrajes conservadosinfo:eu-repograntAgreement/INTA/2023-PE-L01-I037, Desarrollo de sistemas de producción ganaderos climáticamente inteligentes para la Norpatagoniainfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:22Zoai:localhost:20.500.12123/26688instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:23.244INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen Fertilization
title Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen Fertilization
spellingShingle Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen Fertilization
Oyharcabal, Estefanía
Festuca arundinacea
Lotus
Fertilizer Application
Symbionts
Nitrogen Fertilizers
Arbuscular Mycorrhiza
Rhizobiaceae
Organic Fertilizers
Aplicación de Abonos
Simbiontico
Abonos Nitrogenados
Micorriza Arbuscular
Abonos Orgánicos
title_short Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen Fertilization
title_full Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen Fertilization
title_fullStr Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen Fertilization
title_full_unstemmed Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen Fertilization
title_sort Root Symbionts Contributions to Productivity of Grass–Legume Mixtures Under Organic Versus Synthetic Nitrogen Fertilization
dc.creator.none.fl_str_mv Oyharcabal, Estefanía
Covacevich, Fernanda
Bain, Ingrid
Acuña, Claudina Soledad
Berone, German Dario
author Oyharcabal, Estefanía
author_facet Oyharcabal, Estefanía
Covacevich, Fernanda
Bain, Ingrid
Acuña, Claudina Soledad
Berone, German Dario
author_role author
author2 Covacevich, Fernanda
Bain, Ingrid
Acuña, Claudina Soledad
Berone, German Dario
author2_role author
author
author
author
dc.subject.none.fl_str_mv Festuca arundinacea
Lotus
Fertilizer Application
Symbionts
Nitrogen Fertilizers
Arbuscular Mycorrhiza
Rhizobiaceae
Organic Fertilizers
Aplicación de Abonos
Simbiontico
Abonos Nitrogenados
Micorriza Arbuscular
Abonos Orgánicos
topic Festuca arundinacea
Lotus
Fertilizer Application
Symbionts
Nitrogen Fertilizers
Arbuscular Mycorrhiza
Rhizobiaceae
Organic Fertilizers
Aplicación de Abonos
Simbiontico
Abonos Nitrogenados
Micorriza Arbuscular
Abonos Orgánicos
dc.description.none.fl_txt_mv This study assessed the effects of nitrogen (N) fertilization source on forage yield, nutritional status, and the activity/abundance of autochthonous root symbionts in fescue–lotus mixtures. Specifically, the contribution of arbuscular mycorrhizal fungi (AMF) and N-fixing rhizobia (NFR) was assessed under manure (M) and urea (U) fertilization. The aim was to determine whether autochthonous root symbiont populations enhance pasture productivity and nutritional status, mainly under organic fertilization. A nine-month pot experiment was conducted growing fescue–lotus mixtures under two soil conditions: A-soil, with autochthonous root symbiont populations; and R-soil, in which root symbiont populations (AMF and NFR) were reduced via formaldehyde application + UV treatment. Both soil conditions received either no fertilizer (Control) or equal N doses (120 kg N ha− 1 y− 1) supplied as cattle dry manure or urea. Fertilization with M resulted in root symbionts activity/abundance similar to the Control. Fertilization with U reduced root mycorrhizal colonization and nodulation. Plant growth was significantly higher in A-soil than in R-soil, with the legume component showing positive response. Under R-soil conditions, U fertilization resulted in the highest pasture growth, favoring grasses at the expense of legumes. Overall, M appears to be a more suitable fertilizer than U, as it better preserves root symbionts and may also contribute to increased productivity in the mixture. These findings suggest that M may be less disruptive to the studied root symbionts (AMF and NFR) than U under these conditions. These findings could have implications for the development of management strategies designed to preserve potentially beneficial root symbionts in pasture-based livestock systems.
EEA Chubut
Fil: Oyharcabal, Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Oyharcabal, Estefanía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; Argentina
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Bain, Ingrid. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; Argentina
Fil: Acuña, Claudina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; Argentina
Fil: Berone, German Dario. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Berone, German Dario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
description This study assessed the effects of nitrogen (N) fertilization source on forage yield, nutritional status, and the activity/abundance of autochthonous root symbionts in fescue–lotus mixtures. Specifically, the contribution of arbuscular mycorrhizal fungi (AMF) and N-fixing rhizobia (NFR) was assessed under manure (M) and urea (U) fertilization. The aim was to determine whether autochthonous root symbiont populations enhance pasture productivity and nutritional status, mainly under organic fertilization. A nine-month pot experiment was conducted growing fescue–lotus mixtures under two soil conditions: A-soil, with autochthonous root symbiont populations; and R-soil, in which root symbiont populations (AMF and NFR) were reduced via formaldehyde application + UV treatment. Both soil conditions received either no fertilizer (Control) or equal N doses (120 kg N ha− 1 y− 1) supplied as cattle dry manure or urea. Fertilization with M resulted in root symbionts activity/abundance similar to the Control. Fertilization with U reduced root mycorrhizal colonization and nodulation. Plant growth was significantly higher in A-soil than in R-soil, with the legume component showing positive response. Under R-soil conditions, U fertilization resulted in the highest pasture growth, favoring grasses at the expense of legumes. Overall, M appears to be a more suitable fertilizer than U, as it better preserves root symbionts and may also contribute to increased productivity in the mixture. These findings suggest that M may be less disruptive to the studied root symbionts (AMF and NFR) than U under these conditions. These findings could have implications for the development of management strategies designed to preserve potentially beneficial root symbionts in pasture-based livestock systems.
publishDate 2026
dc.date.none.fl_str_mv 2026-06-18T12:43:00Z
2026-06-18T12:43:00Z
2026-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26688
https://link.springer.com/article/10.1007/s42729-026-03367-6
0718-9508
0718-9516
https://doi.org/10.1007/s42729-026-03367-6
url http://hdl.handle.net/20.500.12123/26688
https://link.springer.com/article/10.1007/s42729-026-03367-6
https://doi.org/10.1007/s42729-026-03367-6
identifier_str_mv 0718-9508
0718-9516
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E1-I007-001, Incremento sostenible de la producción y utilización de pasturas y forrajes conservados
info:eu-repograntAgreement/INTA/2023-PE-L01-I037, Desarrollo de sistemas de producción ganaderos climáticamente inteligentes para la Norpatagonia
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of Soil Science and Plant Nutrition : 1-12. (Published: 10 June 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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