First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina

Autores
Badaracco, Alejandra; Redes, Jonathan; Bustamante, Karina; Bloch, Norma; Schapovaloff, Maria Elena; Agostini, Juan Pedro
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Huanglongbing (HLB) is considered one of the most important diseases in citrus industry, which currently has no cure. In Argentina, ‘Candidatus Liberibacter asiaticus’ (CLas) has been detected only in plant material. It is transmitted by Diaphorina citri, which is distributed in most of the country’s citrus regions. The aim of this study was to detect the presence of CLas in D. citri in Argentina. A total of 350 samples of D. citri were analyzed between 2012 and 2018 from different geographical locations from the northeast region of Argentina. A nested-PCR method using specific primers were used for the detection of CLas by amplification of a 1160-bp fragment from the 16S rRNA-encoding gene. Three positive samples to CLas of D. citri were detected from Ituzaingó, Corrientes. The positive PCR products were sequenced, and phylogenetic analysis were conducted. The samples were confirmed to belong to CLas. The sequences obtained in this study grouped with CLas sequences plant material from Argentina together with other sequences from the rest of the world. This is the first report of CLas in D. citri in Argentina.
EEA Montecarlo
Fil: Badaracco, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina.
Fil: Redes, Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina.
Fil: Bustamante, Karina. Universidad Nacional de Misiones. Facultad de Ciencias Forestales; Argentina
Fil: Bloch, Norma. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Centro Regional NEA; Argentina
Fil: Schapovaloff, María E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina
Fil: Agostini, Juan P. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina
Fuente
Australasian Plant Pathology 51 : 9-12. (2022)
Materia
Huanglongbing de los Cítricos
Argentina
Vectores
Diaphorina citri
Identificación
Huanglongbing
Vectors
Identification
Liberibacter
Candidatus Liberibacter
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/27530
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spelling First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in ArgentinaBadaracco, AlejandraRedes, JonathanBustamante, KarinaBloch, NormaSchapovaloff, Maria ElenaAgostini, Juan PedroHuanglongbing de los CítricosArgentinaVectoresDiaphorina citriIdentificaciónHuanglongbingVectorsIdentificationLiberibacterCandidatus LiberibacterHuanglongbing (HLB) is considered one of the most important diseases in citrus industry, which currently has no cure. In Argentina, ‘Candidatus Liberibacter asiaticus’ (CLas) has been detected only in plant material. It is transmitted by Diaphorina citri, which is distributed in most of the country’s citrus regions. The aim of this study was to detect the presence of CLas in D. citri in Argentina. A total of 350 samples of D. citri were analyzed between 2012 and 2018 from different geographical locations from the northeast region of Argentina. A nested-PCR method using specific primers were used for the detection of CLas by amplification of a 1160-bp fragment from the 16S rRNA-encoding gene. Three positive samples to CLas of D. citri were detected from Ituzaingó, Corrientes. The positive PCR products were sequenced, and phylogenetic analysis were conducted. The samples were confirmed to belong to CLas. The sequences obtained in this study grouped with CLas sequences plant material from Argentina together with other sequences from the rest of the world. This is the first report of CLas in D. citri in Argentina.EEA MontecarloFil: Badaracco, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina.Fil: Redes, Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina.Fil: Bustamante, Karina. Universidad Nacional de Misiones. Facultad de Ciencias Forestales; ArgentinaFil: Bloch, Norma. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Centro Regional NEA; ArgentinaFil: Schapovaloff, María E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; ArgentinaFil: Agostini, Juan P. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; ArgentinaThe Australasian Plant Pathology Society2026-08-25T13:10:36Z2026-08-25T13:10:36Z2021-11-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27530https://link.springer.com/article/10.1007/s13313-021-00817-wBadaracco, A., Redes, F.J., Bustamente, K.M. et al. First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina. Australasian Plant Pathol. 51, 9–12 (2022). https://doi.org/10.1007/s13313-021-00817-w1448-60320815-3191https://doi.org/10.1007/s13313-021-00817-wAustralasian Plant Pathology 51 : 9-12. (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:43Zoai:localhost:20.500.12123/27530instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:44.885INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina
title First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina
spellingShingle First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina
Badaracco, Alejandra
Huanglongbing de los Cítricos
Argentina
Vectores
Diaphorina citri
Identificación
Huanglongbing
Vectors
Identification
Liberibacter
Candidatus Liberibacter
title_short First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina
title_full First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina
title_fullStr First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina
title_full_unstemmed First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina
title_sort First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina
dc.creator.none.fl_str_mv Badaracco, Alejandra
Redes, Jonathan
Bustamante, Karina
Bloch, Norma
Schapovaloff, Maria Elena
Agostini, Juan Pedro
author Badaracco, Alejandra
author_facet Badaracco, Alejandra
Redes, Jonathan
Bustamante, Karina
Bloch, Norma
Schapovaloff, Maria Elena
Agostini, Juan Pedro
author_role author
author2 Redes, Jonathan
Bustamante, Karina
Bloch, Norma
Schapovaloff, Maria Elena
Agostini, Juan Pedro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Huanglongbing de los Cítricos
Argentina
Vectores
Diaphorina citri
Identificación
Huanglongbing
Vectors
Identification
Liberibacter
Candidatus Liberibacter
topic Huanglongbing de los Cítricos
Argentina
Vectores
Diaphorina citri
Identificación
Huanglongbing
Vectors
Identification
Liberibacter
Candidatus Liberibacter
dc.description.none.fl_txt_mv Huanglongbing (HLB) is considered one of the most important diseases in citrus industry, which currently has no cure. In Argentina, ‘Candidatus Liberibacter asiaticus’ (CLas) has been detected only in plant material. It is transmitted by Diaphorina citri, which is distributed in most of the country’s citrus regions. The aim of this study was to detect the presence of CLas in D. citri in Argentina. A total of 350 samples of D. citri were analyzed between 2012 and 2018 from different geographical locations from the northeast region of Argentina. A nested-PCR method using specific primers were used for the detection of CLas by amplification of a 1160-bp fragment from the 16S rRNA-encoding gene. Three positive samples to CLas of D. citri were detected from Ituzaingó, Corrientes. The positive PCR products were sequenced, and phylogenetic analysis were conducted. The samples were confirmed to belong to CLas. The sequences obtained in this study grouped with CLas sequences plant material from Argentina together with other sequences from the rest of the world. This is the first report of CLas in D. citri in Argentina.
EEA Montecarlo
Fil: Badaracco, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina.
Fil: Redes, Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina.
Fil: Bustamante, Karina. Universidad Nacional de Misiones. Facultad de Ciencias Forestales; Argentina
Fil: Bloch, Norma. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Centro Regional NEA; Argentina
Fil: Schapovaloff, María E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina
Fil: Agostini, Juan P. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina
description Huanglongbing (HLB) is considered one of the most important diseases in citrus industry, which currently has no cure. In Argentina, ‘Candidatus Liberibacter asiaticus’ (CLas) has been detected only in plant material. It is transmitted by Diaphorina citri, which is distributed in most of the country’s citrus regions. The aim of this study was to detect the presence of CLas in D. citri in Argentina. A total of 350 samples of D. citri were analyzed between 2012 and 2018 from different geographical locations from the northeast region of Argentina. A nested-PCR method using specific primers were used for the detection of CLas by amplification of a 1160-bp fragment from the 16S rRNA-encoding gene. Three positive samples to CLas of D. citri were detected from Ituzaingó, Corrientes. The positive PCR products were sequenced, and phylogenetic analysis were conducted. The samples were confirmed to belong to CLas. The sequences obtained in this study grouped with CLas sequences plant material from Argentina together with other sequences from the rest of the world. This is the first report of CLas in D. citri in Argentina.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-29
2026-08-25T13:10:36Z
2026-08-25T13:10:36Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27530
https://link.springer.com/article/10.1007/s13313-021-00817-w
Badaracco, A., Redes, F.J., Bustamente, K.M. et al. First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina. Australasian Plant Pathol. 51, 9–12 (2022). https://doi.org/10.1007/s13313-021-00817-w
1448-6032
0815-3191
https://doi.org/10.1007/s13313-021-00817-w
url http://hdl.handle.net/20.500.12123/27530
https://link.springer.com/article/10.1007/s13313-021-00817-w
https://doi.org/10.1007/s13313-021-00817-w
identifier_str_mv Badaracco, A., Redes, F.J., Bustamente, K.M. et al. First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina. Australasian Plant Pathol. 51, 9–12 (2022). https://doi.org/10.1007/s13313-021-00817-w
1448-6032
0815-3191
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Australasian Plant Pathology Society
publisher.none.fl_str_mv The Australasian Plant Pathology Society
dc.source.none.fl_str_mv Australasian Plant Pathology 51 : 9-12. (2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.733355

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