Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine...

Autores: Del Medico Zajac, Maria Paula; Zanetti, Flavia Adriana; Federico, Carlos Rodolfo; Zabal, Osvaldo Alfredo; Valera, Alejandro Rafael; Calamante, Gabriela
Año de publicación: 2017
Idioma: inglés
Tipo de recurso: artículo
Estado: versión aceptada
Descripción: In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level.
Inst. de Biotecnología
Fil: Del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esusy, María Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Federico, Carlos Rodolfo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Valera, Alejandro Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Virología; Argentina
Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente: Viral immunology 30 (1) : 70-76. (2017)
Materia: Enfermedades de los Animales
Respuesta inmunológica
Ratón
Herpes Virus Bovino
Glicoproteínas
Immune response
Animal Diseases
Mice
Bovine herpesvirus
Glycoproteins
Nivel de accesibilidad: acceso restringido
Condiciones de uso
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/960

Acceder

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spelling	Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein DDel Medico Zajac, Maria PaulaZanetti, Flavia AdrianaFederico, Carlos RodolfoZabal, Osvaldo AlfredoValera, Alejandro RafaelCalamante, GabrielaEnfermedades de los AnimalesRespuesta inmunológicaRatónHerpes Virus BovinoGlicoproteínasImmune responseAnimal DiseasesMiceBovine herpesvirusGlycoproteinsIn this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level.Inst. de BiotecnologíaFil: Del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Esusy, María Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Federico, Carlos Rodolfo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Valera, Alejandro Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Virología; ArgentinaFil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina2017-08-14T12:49:13Z2017-08-14T12:49:13Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/960http://online.liebertpub.com/doi/10.1089/vim.2016.00901557-8976 (Online)0882-8245 (Print)https://doi.org/10.1089/vim.2016.0090Viral immunology 30 (1) : 70-76. (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2026-09-24T11:32:56Zoai:localhost:20.500.12123/960instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:32:58.162INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D
title	Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D
spellingShingle	Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D Del Medico Zajac, Maria Paula Enfermedades de los Animales Respuesta inmunológica Ratón Herpes Virus Bovino Glicoproteínas Immune response Animal Diseases Mice Bovine herpesvirus Glycoproteins
title_short	Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D
title_full	Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D
title_fullStr	Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D
title_full_unstemmed	Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D
title_sort	Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D
dc.creator.none.fl_str_mv	Del Medico Zajac, Maria Paula Zanetti, Flavia Adriana Federico, Carlos Rodolfo Zabal, Osvaldo Alfredo Valera, Alejandro Rafael Calamante, Gabriela
author	Del Medico Zajac, Maria Paula
author_facet	Del Medico Zajac, Maria Paula Zanetti, Flavia Adriana Federico, Carlos Rodolfo Zabal, Osvaldo Alfredo Valera, Alejandro Rafael Calamante, Gabriela
author_role	author
author2	Zanetti, Flavia Adriana Federico, Carlos Rodolfo Zabal, Osvaldo Alfredo Valera, Alejandro Rafael Calamante, Gabriela
author2_role	author author author author author
dc.subject.none.fl_str_mv	Enfermedades de los Animales Respuesta inmunológica Ratón Herpes Virus Bovino Glicoproteínas Immune response Animal Diseases Mice Bovine herpesvirus Glycoproteins
topic	Enfermedades de los Animales Respuesta inmunológica Ratón Herpes Virus Bovino Glicoproteínas Immune response Animal Diseases Mice Bovine herpesvirus Glycoproteins
dc.description.none.fl_txt_mv	In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level. Inst. de Biotecnología Fil: Del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Esusy, María Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Federico, Carlos Rodolfo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Valera, Alejandro Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Virología; Argentina Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description	In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level.
publishDate	2017
dc.date.none.fl_str_mv	2017-08-14T12:49:13Z 2017-08-14T12:49:13Z 2017
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/960 http://online.liebertpub.com/doi/10.1089/vim.2016.0090 1557-8976 (Online) 0882-8245 (Print) https://doi.org/10.1089/vim.2016.0090
url	http://hdl.handle.net/20.500.12123/960 http://online.liebertpub.com/doi/10.1089/vim.2016.0090 https://doi.org/10.1089/vim.2016.0090
identifier_str_mv	1557-8976 (Online) 0882-8245 (Print)
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv	restrictedAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	Viral immunology 30 (1) : 70-76. (2017) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
collection	INTA Digital (INTA)
instname_str	Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv	INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine...

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