Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix

Autores
Fadiya, Oluwadamilola Victoria; Perillo, Maria Angelica
Año de publicación
2026
Idioma
inglés
Tipo de recurso
conjunto de datos
Estado
versión publicada
Descripción
The files uploaded contain the crude data and a short description of the experiment.
Fil: Fadiya, Oluwadamilola Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Fadiya, Oluwadamilola Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
Fil: Perillo, Maria Angelica. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
In this study, a sol-gel silica matrix was used in repeated batch fermentations for Lactic acid (LA) production, as a carrier of confined Lactiplantibacillus plantarum, previously transformed by incorporating a plasmid containing the gene encoding for E.coli ?-galactosidase (?-Gal). The overexpression of ?-Gal and the increased LA production in transformed L.plantarum, compared with the wild-type bacteria, was confirmed. The parameters involved in lactose fermentation, such as agitation, pH, and temperature involved in the fermentation process, were optimized to improve lactose conversion, reduce fermentation rates, and achieve higher LA productivity by entrapped cells compared to the free cells. LA concentration, yield, and productivity were compared in terms of LA output. The effect of confinement on the cell viability was studied after seven cycles, and minimal loss in bacterial count was observed. Entrapped t-bacteria could be kept preferably at 4ºC for at least 3 months. Immobilized cells reached an LA yield up to 20.7g/L while the free cells reached 18g/L under the same optimized conditions. LA yield depended on the nutrient level of the culture media: broth > whey > lactose solution.
Fil: Fadiya, Oluwadamilola Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Fadiya, Oluwadamilola Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
Fil: Perillo, Maria Angelica. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
Materia
Cell immobilization
Entrapment
Lactiplantibacillus plantarum
Sol-gel mesoporous silicate matrix
Lactic acid production
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/563520
Acceder
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oai_identifier_str oai:rdu.unc.edu.ar:11086/563520
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrixFadiya, Oluwadamilola VictoriaPerillo, Maria AngelicaCell immobilizationEntrapmentLactiplantibacillus plantarumSol-gel mesoporous silicate matrixLactic acid productionhttps://purl.org/becyt/ford/1.6The files uploaded contain the crude data and a short description of the experiment.Fil: Fadiya, Oluwadamilola Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Fadiya, Oluwadamilola Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.Fil: Perillo, Maria Angelica. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.In this study, a sol-gel silica matrix was used in repeated batch fermentations for Lactic acid (LA) production, as a carrier of confined Lactiplantibacillus plantarum, previously transformed by incorporating a plasmid containing the gene encoding for E.coli ?-galactosidase (?-Gal). The overexpression of ?-Gal and the increased LA production in transformed L.plantarum, compared with the wild-type bacteria, was confirmed. The parameters involved in lactose fermentation, such as agitation, pH, and temperature involved in the fermentation process, were optimized to improve lactose conversion, reduce fermentation rates, and achieve higher LA productivity by entrapped cells compared to the free cells. LA concentration, yield, and productivity were compared in terms of LA output. The effect of confinement on the cell viability was studied after seven cycles, and minimal loss in bacterial count was observed. Entrapped t-bacteria could be kept preferably at 4ºC for at least 3 months. Immobilized cells reached an LA yield up to 20.7g/L while the free cells reached 18g/L under the same optimized conditions. LA yield depended on the nutrient level of the culture media: broth > whey > lactose solution.Fil: Fadiya, Oluwadamilola Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Fadiya, Oluwadamilola Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.Fil: Perillo, Maria Angelica. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.https://orcid.org/0000-0002-9836-27332026-10-21info:eu-repo/semantics/dataSetinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_ddb1info:ar-repo/semantics/conjuntoDeDatosapplication/jsontext/xmlapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentFadiya, O., V., Perillo, M. A. (2024) Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix. Consejo Nacional de Investigaciones Científicas y Técnicas. http://hdl.handle.net/11086/563520http://hdl.handle.net/11086/563520engARGinfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2026-10-29T10:18:28Zoai:rdu.unc.edu.ar:11086/563520Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722026-10-29 10:18:28.335Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix
title Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix
spellingShingle Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix
Fadiya, Oluwadamilola Victoria
Cell immobilization
Entrapment
Lactiplantibacillus plantarum
Sol-gel mesoporous silicate matrix
Lactic acid production
title_short Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix
title_full Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix
title_fullStr Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix
title_full_unstemmed Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix
title_sort Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix
dc.creator.none.fl_str_mv Fadiya, Oluwadamilola Victoria
Perillo, Maria Angelica
author Fadiya, Oluwadamilola Victoria
author_facet Fadiya, Oluwadamilola Victoria
Perillo, Maria Angelica
author_role author
author2 Perillo, Maria Angelica
author2_role author
dc.contributor.none.fl_str_mv https://orcid.org/0000-0002-9836-2733
dc.subject.none.fl_str_mv Cell immobilization
Entrapment
Lactiplantibacillus plantarum
Sol-gel mesoporous silicate matrix
Lactic acid production
topic Cell immobilization
Entrapment
Lactiplantibacillus plantarum
Sol-gel mesoporous silicate matrix
Lactic acid production
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv The files uploaded contain the crude data and a short description of the experiment.
Fil: Fadiya, Oluwadamilola Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Fadiya, Oluwadamilola Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
Fil: Perillo, Maria Angelica. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
In this study, a sol-gel silica matrix was used in repeated batch fermentations for Lactic acid (LA) production, as a carrier of confined Lactiplantibacillus plantarum, previously transformed by incorporating a plasmid containing the gene encoding for E.coli ?-galactosidase (?-Gal). The overexpression of ?-Gal and the increased LA production in transformed L.plantarum, compared with the wild-type bacteria, was confirmed. The parameters involved in lactose fermentation, such as agitation, pH, and temperature involved in the fermentation process, were optimized to improve lactose conversion, reduce fermentation rates, and achieve higher LA productivity by entrapped cells compared to the free cells. LA concentration, yield, and productivity were compared in terms of LA output. The effect of confinement on the cell viability was studied after seven cycles, and minimal loss in bacterial count was observed. Entrapped t-bacteria could be kept preferably at 4ºC for at least 3 months. Immobilized cells reached an LA yield up to 20.7g/L while the free cells reached 18g/L under the same optimized conditions. LA yield depended on the nutrient level of the culture media: broth > whey > lactose solution.
Fil: Fadiya, Oluwadamilola Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Fadiya, Oluwadamilola Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
Fil: Perillo, Maria Angelica. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
description The files uploaded contain the crude data and a short description of the experiment.
publishDate 2026
dc.date.none.fl_str_mv 2026-10-21
dc.type.none.fl_str_mv info:eu-repo/semantics/dataSet
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_ddb1
info:ar-repo/semantics/conjuntoDeDatos
format dataSet
status_str publishedVersion
dc.identifier.none.fl_str_mv Fadiya, O., V., Perillo, M. A. (2024) Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix. Consejo Nacional de Investigaciones Científicas y Técnicas. http://hdl.handle.net/11086/563520
http://hdl.handle.net/11086/563520
identifier_str_mv Fadiya, O., V., Perillo, M. A. (2024) Production of lactic acid from whey lactose by Lactiplantibacillus plantarum cells entrapped in a mesoporous silicate matrix. Consejo Nacional de Investigaciones Científicas y Técnicas. http://hdl.handle.net/11086/563520
url http://hdl.handle.net/11086/563520
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/json
text/xml
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
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dc.coverage.none.fl_str_mv ARG
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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