Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybrids

Autores
Diaz de Barboza, Gabriela; Rodríguez, Valeria; Ponce, Rubén Hugo; Theiler, Gerardo Raúl; Maldonado, Cristina; Tolosa de Talamoni, Nori
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Díaz de Barboza, Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Fil: Rodríguez, Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica"A"; Argentina.
Fil: Theiler, Gerardo Raúl. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina.
Fil: Tolosa de Talamoni, Nori. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Spermatogenesis is disrupted in Graomys griseoflavus × Graomys centralis male hybrids. This study was aimed to determine whether morphological alterations in Leydig cells from hybrids accompany the arrest of spermatogenesis and cell death of germ cells and whether apoptotic pathways are also involved in the response of these interstitial cells. We used three groups of 1-, 2- and 3-month-old male animals: (1) G. centralis, (2) G. griseoflavus and (3) hybrids obtained by crossing G. griseoflavus females with G. centralis males. Testicular ultrastructure was analyzed by transmission electron microscopy. TUNEL was studied using an in situ cell death detection kit and the expression of apoptotic molecules by immunohistochemistry. The data confirmed arrest of spermatogenesis and intense apoptotic processes of germ cells in hybrids. These animals also showed ultrastructural alterations in the Leydig cells. Fas, FasL and calbindin D28k overexpression without an increase in DNA fragmentation was detected in the Leydig cells from hybrids. In conclusion, the sterility of Graomys hybrids occurs with ultrastructural changes in germ and Leydig cells. The enhancement of Fas and FasL is not associated with cell death in the Leydig cells. Probably the apoptosis in these interstitial cells is inhibited by the high expression of the antiapoptotic molecule calbindin D28k.
http://www.sciencedirect.com/science/article/pii/S0065128114000762
publishedVersion
Fil: Díaz de Barboza, Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Fil: Rodríguez, Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica"A"; Argentina.
Fil: Theiler, Gerardo Raúl. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina.
Fil: Tolosa de Talamoni, Nori. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Biología Reproductiva (aspectos médicos van en 3 "Ciencias Médicas y de la Hídrico Salud")
Materia
Graomys griseoflavus
Graomys centralis
Hybrids
Leydig cells
Germ cells
Testes
Ultrastucture
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/562613
Acceder
id RDUUNC_7fa4b88e86787be29be0802044c0bf46
oai_identifier_str oai:rdu.unc.edu.ar:11086/562613
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybridsDiaz de Barboza, GabrielaRodríguez, ValeriaPonce, Rubén HugoTheiler, Gerardo RaúlMaldonado, CristinaTolosa de Talamoni, NoriGraomys griseoflavusGraomys centralisHybridsLeydig cellsGerm cellsTestesUltrastuctureFil: Díaz de Barboza, Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.Fil: Rodríguez, Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica"A"; Argentina.Fil: Theiler, Gerardo Raúl. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina.Fil: Tolosa de Talamoni, Nori. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.Spermatogenesis is disrupted in Graomys griseoflavus × Graomys centralis male hybrids. This study was aimed to determine whether morphological alterations in Leydig cells from hybrids accompany the arrest of spermatogenesis and cell death of germ cells and whether apoptotic pathways are also involved in the response of these interstitial cells. We used three groups of 1-, 2- and 3-month-old male animals: (1) G. centralis, (2) G. griseoflavus and (3) hybrids obtained by crossing G. griseoflavus females with G. centralis males. Testicular ultrastructure was analyzed by transmission electron microscopy. TUNEL was studied using an in situ cell death detection kit and the expression of apoptotic molecules by immunohistochemistry. The data confirmed arrest of spermatogenesis and intense apoptotic processes of germ cells in hybrids. These animals also showed ultrastructural alterations in the Leydig cells. Fas, FasL and calbindin D28k overexpression without an increase in DNA fragmentation was detected in the Leydig cells from hybrids. In conclusion, the sterility of Graomys hybrids occurs with ultrastructural changes in germ and Leydig cells. The enhancement of Fas and FasL is not associated with cell death in the Leydig cells. Probably the apoptosis in these interstitial cells is inhibited by the high expression of the antiapoptotic molecule calbindin D28k.http://www.sciencedirect.com/science/article/pii/S0065128114000762publishedVersionFil: Díaz de Barboza, Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.Fil: Rodríguez, Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica"A"; Argentina.Fil: Theiler, Gerardo Raúl. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina.Fil: Tolosa de Talamoni, Nori. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.Biología Reproductiva (aspectos médicos van en 3 "Ciencias Médicas y de la Hídrico Salud")https://orcid.org/0000-0003-0617-8005https://orcid.org/0000-0003-2296-1534https://orcid.org/0009-0007-4378-297Xhttps://orcid.org/0000-0001-5570-20242014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfDíaz de Barboza G, Rodríguez V, Ponce R, Theiler G, Maldonado C, Tolosa de Talamoni N. Association of cellular and molecular alterations in Leydig cells with apoptotic changes in germ cells from testes of Graomys griseoflavus×Graomys centralis male hybrids. Acta Histochem. 2014;116(6):1037–45. Disponible en: http://dx.doi.org/10.1016/j.acthis.2014.04.007http://hdl.handle.net/11086/562613http://dx.doi.org/10.1016/j.acthis.2014.04.007enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2026-09-24T11:35:25Zoai:rdu.unc.edu.ar:11086/562613Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722026-09-24 11:35:26.343Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybrids
title Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybrids
spellingShingle Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybrids
Diaz de Barboza, Gabriela
Graomys griseoflavus
Graomys centralis
Hybrids
Leydig cells
Germ cells
Testes
Ultrastucture
title_short Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybrids
title_full Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybrids
title_fullStr Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybrids
title_full_unstemmed Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybrids
title_sort Association of cellular and molecular alterations in Leydig cells withapoptotic changes in germ cells from testes of Graomysgriseoflavus × Graomys centralis male hybrids
dc.creator.none.fl_str_mv Diaz de Barboza, Gabriela
Rodríguez, Valeria
Ponce, Rubén Hugo
Theiler, Gerardo Raúl
Maldonado, Cristina
Tolosa de Talamoni, Nori
author Diaz de Barboza, Gabriela
author_facet Diaz de Barboza, Gabriela
Rodríguez, Valeria
Ponce, Rubén Hugo
Theiler, Gerardo Raúl
Maldonado, Cristina
Tolosa de Talamoni, Nori
author_role author
author2 Rodríguez, Valeria
Ponce, Rubén Hugo
Theiler, Gerardo Raúl
Maldonado, Cristina
Tolosa de Talamoni, Nori
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv https://orcid.org/0000-0003-0617-8005
https://orcid.org/0000-0003-2296-1534
https://orcid.org/0009-0007-4378-297X
https://orcid.org/0000-0001-5570-2024
dc.subject.none.fl_str_mv Graomys griseoflavus
Graomys centralis
Hybrids
Leydig cells
Germ cells
Testes
Ultrastucture
topic Graomys griseoflavus
Graomys centralis
Hybrids
Leydig cells
Germ cells
Testes
Ultrastucture
dc.description.none.fl_txt_mv Fil: Díaz de Barboza, Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Fil: Rodríguez, Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica"A"; Argentina.
Fil: Theiler, Gerardo Raúl. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina.
Fil: Tolosa de Talamoni, Nori. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Spermatogenesis is disrupted in Graomys griseoflavus × Graomys centralis male hybrids. This study was aimed to determine whether morphological alterations in Leydig cells from hybrids accompany the arrest of spermatogenesis and cell death of germ cells and whether apoptotic pathways are also involved in the response of these interstitial cells. We used three groups of 1-, 2- and 3-month-old male animals: (1) G. centralis, (2) G. griseoflavus and (3) hybrids obtained by crossing G. griseoflavus females with G. centralis males. Testicular ultrastructure was analyzed by transmission electron microscopy. TUNEL was studied using an in situ cell death detection kit and the expression of apoptotic molecules by immunohistochemistry. The data confirmed arrest of spermatogenesis and intense apoptotic processes of germ cells in hybrids. These animals also showed ultrastructural alterations in the Leydig cells. Fas, FasL and calbindin D28k overexpression without an increase in DNA fragmentation was detected in the Leydig cells from hybrids. In conclusion, the sterility of Graomys hybrids occurs with ultrastructural changes in germ and Leydig cells. The enhancement of Fas and FasL is not associated with cell death in the Leydig cells. Probably the apoptosis in these interstitial cells is inhibited by the high expression of the antiapoptotic molecule calbindin D28k.
http://www.sciencedirect.com/science/article/pii/S0065128114000762
publishedVersion
Fil: Díaz de Barboza, Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Fil: Rodríguez, Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica"A"; Argentina.
Fil: Theiler, Gerardo Raúl. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina.
Fil: Tolosa de Talamoni, Nori. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
Biología Reproductiva (aspectos médicos van en 3 "Ciencias Médicas y de la Hídrico Salud")
description Fil: Díaz de Barboza, Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra Bioquímica y Biología Molecular; Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Díaz de Barboza G, Rodríguez V, Ponce R, Theiler G, Maldonado C, Tolosa de Talamoni N. Association of cellular and molecular alterations in Leydig cells with apoptotic changes in germ cells from testes of Graomys griseoflavus×Graomys centralis male hybrids. Acta Histochem. 2014;116(6):1037–45. Disponible en: http://dx.doi.org/10.1016/j.acthis.2014.04.007
http://hdl.handle.net/11086/562613
http://dx.doi.org/10.1016/j.acthis.2014.04.007
identifier_str_mv Díaz de Barboza G, Rodríguez V, Ponce R, Theiler G, Maldonado C, Tolosa de Talamoni N. Association of cellular and molecular alterations in Leydig cells with apoptotic changes in germ cells from testes of Graomys griseoflavus×Graomys centralis male hybrids. Acta Histochem. 2014;116(6):1037–45. Disponible en: http://dx.doi.org/10.1016/j.acthis.2014.04.007
url http://hdl.handle.net/11086/562613
http://dx.doi.org/10.1016/j.acthis.2014.04.007
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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score 12.733355

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