Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.

Autores
Pérez Pizá, María Cecilia; Ibañez, Verónica; Varela, Anabella; Ferreyra, Matías; Chamorro, Juan Camilo; Zilli, Carla; Vallecorsa, Pablo; Fina, Brenda; Prevosto, Leandro; Marfil, Carlos; Cejas, Ezequiel; Balestrasse, Karina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Non-thermal plasmas (NTP) are partially ionized gases that represent a promising technology for seed treatment to enhance seed health while promoting germination and vigor in a fast, cost-efective, and eco-friendly way. The seed treatment with NTP generates phenotypic variations in plants that could be related to changes in DNA methylation. This work analyses the efects of two diferent NTP: nitrogen for 3 min (PMN3) and oxygen for 2 min (PMO2) applied to soybean (Glycine max) seeds. Growth parameters of plants grown from treated and untreated seeds were evaluated at two growth stages: 6 and 20 days after sowing (DAS). MSAP (Methylation Sensitive Amplifed Polymorphism) markers were assayed to evaluate epigenetic changes induced by NTP treatments. Plants obtained from PMN3 and PMO2-treated seeds were phenotypically similar to each other: exhibited a superior growth at both stages. At 6 DAS root and shoot length and fresh weight surpassed the Control, while at 20 DAS root length and fresh and dry weight were higher than Control. PMN3 and PMO2 induced DNA methylation changes with respect to the Control plants, with higher diferentiation at 20 DAS than at 6 DAS. The epigenetic variability and the phenotypic variability correlated only at 20 DAS (R2=0.5). The observed phenotypic diferences among Control and NTP-treated plants could not be explained by overall changes in the methylation levels, but both demethylation and methylation changes at specifc loci appear to be operating in response to NTP treatments.
Fil: Pérez Pizá, María Cecilia. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.
Fil: Ibañez, Verónica. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza. CONICET. Argentina.
Fil: Varela, Anabella. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza. CONICET. Argentina.
Fil: Cejas, Ezequiel. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. CONICET. Santa Fe; Argentina.
Fil: Ferreyra, Matías. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. Santa Fe; Argentina.
Fil: Chamorro, Juan Camilo. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. Santa Fe; Argentina.
Fil: Zilli, Carla. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.
Fil: Vallecorsa, Pablo. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.
Fil: Fina, Brenda. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. Santa Fe; Argentina.
Fil: Prevosto, Leandro. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. CONICET. Santa Fe; Argentina.
Fil: Marfil, Carlos. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza. CONICET. Argentina.
Fil: Balestrasse, Karina. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.
Peer Reviewed
Fuente
Volume 41, pages 2732–2742, (2022).
Materia
MSAP.
Epigenetics.
Soybean.
Non-thermal plasma.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2024-04-30T22:20:33Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/10695
Acceder
id RIAUTN_21b1bcd055da0fc4ab7a20f75f673848
oai_identifier_str oai:ria.utn.edu.ar:20.500.12272/10695
network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.Pérez Pizá, María CeciliaIbañez, VerónicaVarela, AnabellaFerreyra, MatíasChamorro, Juan CamiloZilli, CarlaVallecorsa, PabloFina, BrendaPrevosto, LeandroMarfil, CarlosCejas, EzequielBalestrasse, KarinaMSAP.Epigenetics.Soybean.Non-thermal plasma.Non-thermal plasmas (NTP) are partially ionized gases that represent a promising technology for seed treatment to enhance seed health while promoting germination and vigor in a fast, cost-efective, and eco-friendly way. The seed treatment with NTP generates phenotypic variations in plants that could be related to changes in DNA methylation. This work analyses the efects of two diferent NTP: nitrogen for 3 min (PMN3) and oxygen for 2 min (PMO2) applied to soybean (Glycine max) seeds. Growth parameters of plants grown from treated and untreated seeds were evaluated at two growth stages: 6 and 20 days after sowing (DAS). MSAP (Methylation Sensitive Amplifed Polymorphism) markers were assayed to evaluate epigenetic changes induced by NTP treatments. Plants obtained from PMN3 and PMO2-treated seeds were phenotypically similar to each other: exhibited a superior growth at both stages. At 6 DAS root and shoot length and fresh weight surpassed the Control, while at 20 DAS root length and fresh and dry weight were higher than Control. PMN3 and PMO2 induced DNA methylation changes with respect to the Control plants, with higher diferentiation at 20 DAS than at 6 DAS. The epigenetic variability and the phenotypic variability correlated only at 20 DAS (R2=0.5). The observed phenotypic diferences among Control and NTP-treated plants could not be explained by overall changes in the methylation levels, but both demethylation and methylation changes at specifc loci appear to be operating in response to NTP treatments.Fil: Pérez Pizá, María Cecilia. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.Fil: Ibañez, Verónica. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza. CONICET. Argentina.Fil: Varela, Anabella. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza. CONICET. Argentina.Fil: Cejas, Ezequiel. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. CONICET. Santa Fe; Argentina.Fil: Ferreyra, Matías. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. Santa Fe; Argentina.Fil: Chamorro, Juan Camilo. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. Santa Fe; Argentina.Fil: Zilli, Carla. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.Fil: Vallecorsa, Pablo. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.Fil: Fina, Brenda. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. Santa Fe; Argentina.Fil: Prevosto, Leandro. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. CONICET. Santa Fe; Argentina.Fil: Marfil, Carlos. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza. CONICET. Argentina.Fil: Balestrasse, Karina. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.Peer Reviewed2024-04-30T22:20:33Z2024-04-30T22:20:33Z2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articulopdfapplication/pdfJournal of Plant Growth Regulation.http://hdl.handle.net/20.500.12272/10695doi.org/10.1007/s00344-021-10470-8Volume 41, pages 2732–2742, (2022).reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacionalenginfo:eu-repo/semantics/openAccess2024-04-30T22:20:33Zhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 InternacionalPrevosto, Leandro.Creative Commons http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional Este trabajo puede ser utilizado con fines académicos y de estudio.2026-09-24T12:47:46Zoai:ria.utn.edu.ar:20.500.12272/10695instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:47:48.346Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.
title Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.
spellingShingle Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.
Pérez Pizá, María Cecilia
MSAP.
Epigenetics.
Soybean.
Non-thermal plasma.
title_short Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.
title_full Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.
title_fullStr Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.
title_full_unstemmed Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.
title_sort Non‑Thermal Plasmas Afect Plant Growth and DNA Methylation Patterns in Glycine max.
dc.creator.none.fl_str_mv Pérez Pizá, María Cecilia
Ibañez, Verónica
Varela, Anabella
Ferreyra, Matías
Chamorro, Juan Camilo
Zilli, Carla
Vallecorsa, Pablo
Fina, Brenda
Prevosto, Leandro
Marfil, Carlos
Cejas, Ezequiel
Balestrasse, Karina
author Pérez Pizá, María Cecilia
author_facet Pérez Pizá, María Cecilia
Ibañez, Verónica
Varela, Anabella
Ferreyra, Matías
Chamorro, Juan Camilo
Zilli, Carla
Vallecorsa, Pablo
Fina, Brenda
Prevosto, Leandro
Marfil, Carlos
Cejas, Ezequiel
Balestrasse, Karina
author_role author
author2 Ibañez, Verónica
Varela, Anabella
Ferreyra, Matías
Chamorro, Juan Camilo
Zilli, Carla
Vallecorsa, Pablo
Fina, Brenda
Prevosto, Leandro
Marfil, Carlos
Cejas, Ezequiel
Balestrasse, Karina
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MSAP.
Epigenetics.
Soybean.
Non-thermal plasma.
topic MSAP.
Epigenetics.
Soybean.
Non-thermal plasma.
dc.description.none.fl_txt_mv Non-thermal plasmas (NTP) are partially ionized gases that represent a promising technology for seed treatment to enhance seed health while promoting germination and vigor in a fast, cost-efective, and eco-friendly way. The seed treatment with NTP generates phenotypic variations in plants that could be related to changes in DNA methylation. This work analyses the efects of two diferent NTP: nitrogen for 3 min (PMN3) and oxygen for 2 min (PMO2) applied to soybean (Glycine max) seeds. Growth parameters of plants grown from treated and untreated seeds were evaluated at two growth stages: 6 and 20 days after sowing (DAS). MSAP (Methylation Sensitive Amplifed Polymorphism) markers were assayed to evaluate epigenetic changes induced by NTP treatments. Plants obtained from PMN3 and PMO2-treated seeds were phenotypically similar to each other: exhibited a superior growth at both stages. At 6 DAS root and shoot length and fresh weight surpassed the Control, while at 20 DAS root length and fresh and dry weight were higher than Control. PMN3 and PMO2 induced DNA methylation changes with respect to the Control plants, with higher diferentiation at 20 DAS than at 6 DAS. The epigenetic variability and the phenotypic variability correlated only at 20 DAS (R2=0.5). The observed phenotypic diferences among Control and NTP-treated plants could not be explained by overall changes in the methylation levels, but both demethylation and methylation changes at specifc loci appear to be operating in response to NTP treatments.
Fil: Pérez Pizá, María Cecilia. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.
Fil: Ibañez, Verónica. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza. CONICET. Argentina.
Fil: Varela, Anabella. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza. CONICET. Argentina.
Fil: Cejas, Ezequiel. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. CONICET. Santa Fe; Argentina.
Fil: Ferreyra, Matías. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. Santa Fe; Argentina.
Fil: Chamorro, Juan Camilo. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. Santa Fe; Argentina.
Fil: Zilli, Carla. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.
Fil: Vallecorsa, Pablo. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.
Fil: Fina, Brenda. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. Santa Fe; Argentina.
Fil: Prevosto, Leandro. Universidad Tecnológica Nacional. Facultad Regional Venado Tuerto. Grupo de Descargas Eléctricas. Departamento Ingeniería Electromecánica. CONICET. Santa Fe; Argentina.
Fil: Marfil, Carlos. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza. CONICET. Argentina.
Fil: Balestrasse, Karina. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. CONICET. Argentina.
Peer Reviewed
description Non-thermal plasmas (NTP) are partially ionized gases that represent a promising technology for seed treatment to enhance seed health while promoting germination and vigor in a fast, cost-efective, and eco-friendly way. The seed treatment with NTP generates phenotypic variations in plants that could be related to changes in DNA methylation. This work analyses the efects of two diferent NTP: nitrogen for 3 min (PMN3) and oxygen for 2 min (PMO2) applied to soybean (Glycine max) seeds. Growth parameters of plants grown from treated and untreated seeds were evaluated at two growth stages: 6 and 20 days after sowing (DAS). MSAP (Methylation Sensitive Amplifed Polymorphism) markers were assayed to evaluate epigenetic changes induced by NTP treatments. Plants obtained from PMN3 and PMO2-treated seeds were phenotypically similar to each other: exhibited a superior growth at both stages. At 6 DAS root and shoot length and fresh weight surpassed the Control, while at 20 DAS root length and fresh and dry weight were higher than Control. PMN3 and PMO2 induced DNA methylation changes with respect to the Control plants, with higher diferentiation at 20 DAS than at 6 DAS. The epigenetic variability and the phenotypic variability correlated only at 20 DAS (R2=0.5). The observed phenotypic diferences among Control and NTP-treated plants could not be explained by overall changes in the methylation levels, but both demethylation and methylation changes at specifc loci appear to be operating in response to NTP treatments.
publishDate 2021
dc.date.none.fl_str_mv 2021
2024-04-30T22:20:33Z
2024-04-30T22:20:33Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv Journal of Plant Growth Regulation.
http://hdl.handle.net/20.500.12272/10695
doi.org/10.1007/s00344-021-10470-8
identifier_str_mv Journal of Plant Growth Regulation.
doi.org/10.1007/s00344-021-10470-8
url http://hdl.handle.net/20.500.12272/10695
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2024-04-30T22:20:33Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Prevosto, Leandro.
Creative Commons http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional Este trabajo puede ser utilizado con fines académicos y de estudio.
eu_rights_str_mv openAccess
rights_invalid_str_mv 2024-04-30T22:20:33Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Prevosto, Leandro.
Creative Commons http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional Este trabajo puede ser utilizado con fines académicos y de estudio.
dc.format.none.fl_str_mv pdf
application/pdf
dc.source.none.fl_str_mv Volume 41, pages 2732–2742, (2022).
reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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score 13.24418

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