Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.

Autores
del Valle, Juana Cristina; Zanazzi, Aldo Nahuel; Rodriguez, Yamila Eliana; Haran, Nora Selma; Laitano, María Victoria; Mallo, Juan Carlos; Fernández‐Gimenez, Analía Verónica
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During the early ontogeny, the transition from endogenous (yolk protein) to exogenous feeding (artificial diets) represents a critical period linked to the undifferentiated digestive system, with low digestibility of food protein. The objectives of this work were to characterize the morphology of the early Nile tilapia (Oreochromis niloticus) developmental stages and determine the activity of alkaline and acid peptidase enzymes during the ontogenesis from hatching to 20 days post-hatching (DPH). Also, the in vitro effect that exogenous enzymes from Argentine red shrimp (Pleoticus muelleri) waste have on the alkaline peptidases of larvae from 6 to 20 DPH (which correspond to the age at which fish eat exogenous food) was studied. Both acid and alkaline peptidase activities varied throughout early ontogeny development (from 0.1 to 1, and from 0.1 to 7.1 UE mg protein−1, respectively). The patterns of both enzyme activity variation would be related with changes in endogenous, mixed and exogenous feeding. Our studies show that the additions of the enzyme extract of shrimp have a synergistic effect (from 3 to 6 times) on endogenous in vitro activity. Moreover, the zymogram analysis demonstrates that the bands corresponding to the activity of each species (tilapia and red shrimp) remain active when they are mixed. The increase in peptidase digestive capacity by addition of exogenous enzymes would maximize the assimilation of nutrients from artificial food during early development.
Fil: del Valle, Juana Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.
Fil: Haran, Nora Selma. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.
Fil: Laitano, María Victoria. Universidad Nacional de Mar del Plata (Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras); Argentina.
Fil: Fernández‐Gimenez, Analía Verónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.
Fil: Rodriguez, Yamila Eliana. Universidad Tecnológica Nacional. Facultad Regional Mar del Plata; Argentina.
Fil: Rodriguez, Yamila Eliana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.
Fil: Mallo, Juan Carlos. Universidad Tecnológica Nacional. Facultad Regional Mar del Plata; Argentina.
Fil: Mallo, Juan Carlos. Comisión de Investigaciones Científicas; Argentina.
Fil: Zanazzi, Aldo Nahuel. Universidad Tecnológica Nacional. Facultad Regional Mar del Plata; Argentina.
Peer Reviewed
Materia
Tecnicatura en acuicultura y producción pesquera
Tilapia
Digestion
Peptidase
Ontogeny
Enzymes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-10-03T21:53:44Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/7128
Acceder
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network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.del Valle, Juana CristinaZanazzi, Aldo NahuelRodriguez, Yamila ElianaHaran, Nora SelmaLaitano, María VictoriaMallo, Juan CarlosFernández‐Gimenez, Analía VerónicaTecnicatura en acuicultura y producción pesqueraTilapiaDigestionPeptidaseOntogenyEnzymesDuring the early ontogeny, the transition from endogenous (yolk protein) to exogenous feeding (artificial diets) represents a critical period linked to the undifferentiated digestive system, with low digestibility of food protein. The objectives of this work were to characterize the morphology of the early Nile tilapia (Oreochromis niloticus) developmental stages and determine the activity of alkaline and acid peptidase enzymes during the ontogenesis from hatching to 20 days post-hatching (DPH). Also, the in vitro effect that exogenous enzymes from Argentine red shrimp (Pleoticus muelleri) waste have on the alkaline peptidases of larvae from 6 to 20 DPH (which correspond to the age at which fish eat exogenous food) was studied. Both acid and alkaline peptidase activities varied throughout early ontogeny development (from 0.1 to 1, and from 0.1 to 7.1 UE mg protein−1, respectively). The patterns of both enzyme activity variation would be related with changes in endogenous, mixed and exogenous feeding. Our studies show that the additions of the enzyme extract of shrimp have a synergistic effect (from 3 to 6 times) on endogenous in vitro activity. Moreover, the zymogram analysis demonstrates that the bands corresponding to the activity of each species (tilapia and red shrimp) remain active when they are mixed. The increase in peptidase digestive capacity by addition of exogenous enzymes would maximize the assimilation of nutrients from artificial food during early development.Fil: del Valle, Juana Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.Fil: Haran, Nora Selma. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.Fil: Laitano, María Victoria. Universidad Nacional de Mar del Plata (Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras); Argentina.Fil: Fernández‐Gimenez, Analía Verónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.Fil: Rodriguez, Yamila Eliana. Universidad Tecnológica Nacional. Facultad Regional Mar del Plata; Argentina.Fil: Rodriguez, Yamila Eliana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.Fil: Mallo, Juan Carlos. Universidad Tecnológica Nacional. Facultad Regional Mar del Plata; Argentina.Fil: Mallo, Juan Carlos. Comisión de Investigaciones Científicas; Argentina.Fil: Zanazzi, Aldo Nahuel. Universidad Tecnológica Nacional. Facultad Regional Mar del Plata; Argentina.Peer Reviewed2022-10-03T21:53:44Z2022-10-03T21:53:44Z2022-07-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articulopdfapplication/pdfdel Valle, J.C.; Zanazzi, A.N.; Rodriguez, Y.E.; Haran, N.S.; Laitano, M.V.; Mallo, J.C.; Fernández-Gimenez, A.V. (2022) Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus. Aquacult International (30) 1645–1658.http://hdl.handle.net/20.500.12272/7128https://doi.org/10.1007/s10499-022-00932-5enginfo:eu-repo/semantics/openAccess2022-10-03T21:53:44Zhttp://creativecommons.org/licenses/by-nc/4.0/Atribución-NoComercial 4.0 InternacionalReservados todos los derechos. No se permite la reproducción total o parcial de esta obra, ni su incorporación a un sistema informático, ni su transmisión en cualquier forma o por cualquier medio (electrónico, mecánico, fotocopia, grabación u otros) sin autorización previa y por escrito de los titulares del copyright. La infracción de dichos derechos puede constituir un delito contra la propiedad intelectual.reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:45:33Zoai:ria.utn.edu.ar:20.500.12272/7128instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:45:34.148Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.
title Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.
spellingShingle Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.
del Valle, Juana Cristina
Tecnicatura en acuicultura y producción pesquera
Tilapia
Digestion
Peptidase
Ontogeny
Enzymes
title_short Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.
title_full Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.
title_fullStr Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.
title_full_unstemmed Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.
title_sort Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus.
dc.creator.none.fl_str_mv del Valle, Juana Cristina
Zanazzi, Aldo Nahuel
Rodriguez, Yamila Eliana
Haran, Nora Selma
Laitano, María Victoria
Mallo, Juan Carlos
Fernández‐Gimenez, Analía Verónica
author del Valle, Juana Cristina
author_facet del Valle, Juana Cristina
Zanazzi, Aldo Nahuel
Rodriguez, Yamila Eliana
Haran, Nora Selma
Laitano, María Victoria
Mallo, Juan Carlos
Fernández‐Gimenez, Analía Verónica
author_role author
author2 Zanazzi, Aldo Nahuel
Rodriguez, Yamila Eliana
Haran, Nora Selma
Laitano, María Victoria
Mallo, Juan Carlos
Fernández‐Gimenez, Analía Verónica
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Tecnicatura en acuicultura y producción pesquera
Tilapia
Digestion
Peptidase
Ontogeny
Enzymes
topic Tecnicatura en acuicultura y producción pesquera
Tilapia
Digestion
Peptidase
Ontogeny
Enzymes
dc.description.none.fl_txt_mv During the early ontogeny, the transition from endogenous (yolk protein) to exogenous feeding (artificial diets) represents a critical period linked to the undifferentiated digestive system, with low digestibility of food protein. The objectives of this work were to characterize the morphology of the early Nile tilapia (Oreochromis niloticus) developmental stages and determine the activity of alkaline and acid peptidase enzymes during the ontogenesis from hatching to 20 days post-hatching (DPH). Also, the in vitro effect that exogenous enzymes from Argentine red shrimp (Pleoticus muelleri) waste have on the alkaline peptidases of larvae from 6 to 20 DPH (which correspond to the age at which fish eat exogenous food) was studied. Both acid and alkaline peptidase activities varied throughout early ontogeny development (from 0.1 to 1, and from 0.1 to 7.1 UE mg protein−1, respectively). The patterns of both enzyme activity variation would be related with changes in endogenous, mixed and exogenous feeding. Our studies show that the additions of the enzyme extract of shrimp have a synergistic effect (from 3 to 6 times) on endogenous in vitro activity. Moreover, the zymogram analysis demonstrates that the bands corresponding to the activity of each species (tilapia and red shrimp) remain active when they are mixed. The increase in peptidase digestive capacity by addition of exogenous enzymes would maximize the assimilation of nutrients from artificial food during early development.
Fil: del Valle, Juana Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.
Fil: Haran, Nora Selma. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.
Fil: Laitano, María Victoria. Universidad Nacional de Mar del Plata (Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras); Argentina.
Fil: Fernández‐Gimenez, Analía Verónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.
Fil: Rodriguez, Yamila Eliana. Universidad Tecnológica Nacional. Facultad Regional Mar del Plata; Argentina.
Fil: Rodriguez, Yamila Eliana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Consejo Nacional de Investigaciones Cientificas y Tecnologicas. Instituto de Investigaciones Marinas y Costeras; Argentina.
Fil: Mallo, Juan Carlos. Universidad Tecnológica Nacional. Facultad Regional Mar del Plata; Argentina.
Fil: Mallo, Juan Carlos. Comisión de Investigaciones Científicas; Argentina.
Fil: Zanazzi, Aldo Nahuel. Universidad Tecnológica Nacional. Facultad Regional Mar del Plata; Argentina.
Peer Reviewed
description During the early ontogeny, the transition from endogenous (yolk protein) to exogenous feeding (artificial diets) represents a critical period linked to the undifferentiated digestive system, with low digestibility of food protein. The objectives of this work were to characterize the morphology of the early Nile tilapia (Oreochromis niloticus) developmental stages and determine the activity of alkaline and acid peptidase enzymes during the ontogenesis from hatching to 20 days post-hatching (DPH). Also, the in vitro effect that exogenous enzymes from Argentine red shrimp (Pleoticus muelleri) waste have on the alkaline peptidases of larvae from 6 to 20 DPH (which correspond to the age at which fish eat exogenous food) was studied. Both acid and alkaline peptidase activities varied throughout early ontogeny development (from 0.1 to 1, and from 0.1 to 7.1 UE mg protein−1, respectively). The patterns of both enzyme activity variation would be related with changes in endogenous, mixed and exogenous feeding. Our studies show that the additions of the enzyme extract of shrimp have a synergistic effect (from 3 to 6 times) on endogenous in vitro activity. Moreover, the zymogram analysis demonstrates that the bands corresponding to the activity of each species (tilapia and red shrimp) remain active when they are mixed. The increase in peptidase digestive capacity by addition of exogenous enzymes would maximize the assimilation of nutrients from artificial food during early development.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-03T21:53:44Z
2022-10-03T21:53:44Z
2022-07-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv del Valle, J.C.; Zanazzi, A.N.; Rodriguez, Y.E.; Haran, N.S.; Laitano, M.V.; Mallo, J.C.; Fernández-Gimenez, A.V. (2022) Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus. Aquacult International (30) 1645–1658.
http://hdl.handle.net/20.500.12272/7128
https://doi.org/10.1007/s10499-022-00932-5
identifier_str_mv del Valle, J.C.; Zanazzi, A.N.; Rodriguez, Y.E.; Haran, N.S.; Laitano, M.V.; Mallo, J.C.; Fernández-Gimenez, A.V. (2022) Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus. Aquacult International (30) 1645–1658.
url http://hdl.handle.net/20.500.12272/7128
https://doi.org/10.1007/s10499-022-00932-5
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-10-03T21:53:44Z
http://creativecommons.org/licenses/by-nc/4.0/
Atribución-NoComercial 4.0 Internacional
Reservados todos los derechos. No se permite la reproducción total o parcial de esta obra, ni su incorporación a un sistema informático, ni su transmisión en cualquier forma o por cualquier medio (electrónico, mecánico, fotocopia, grabación u otros) sin autorización previa y por escrito de los titulares del copyright. La infracción de dichos derechos puede constituir un delito contra la propiedad intelectual.
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-10-03T21:53:44Z
http://creativecommons.org/licenses/by-nc/4.0/
Atribución-NoComercial 4.0 Internacional
Reservados todos los derechos. No se permite la reproducción total o parcial de esta obra, ni su incorporación a un sistema informático, ni su transmisión en cualquier forma o por cualquier medio (electrónico, mecánico, fotocopia, grabación u otros) sin autorización previa y por escrito de los titulares del copyright. La infracción de dichos derechos puede constituir un delito contra la propiedad intelectual.
dc.format.none.fl_str_mv pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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score 13.265058

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