Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel production

Autores
Ferrero, Gabriel O.; Sánchez Faba, Edgar M.; Eimer, Griselda Alejandra
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The aim of this work is to design a catalyst for biofuels production from renewable raw materials by means of the immobilization of an enzyme on nano-structured solid supports [1,2]. In fact, the main purpose is to immobilize the lipase of Pseudomonas fluorescens in the pure SBA-15 (Santa Barbara Amorphous) mesoporous material [3] and to compare their activity in the biodiesel production using vegetable oils, with that of the enzyme immobilized on materials SBA-15 modified with metals (Ca, Na, Fe, Cu, Al, K)[4]. The physicochemical properties of the synthesized mesoporous materials were determined by Small-angle X-ray scattering (SAXS), Transmission electron microscopy (TEM) and UV-visible. The enzyme was immobilized by physical adsorption, mixing each mesoporous material with an enzyme solution. The effective incorporation of the enzyme in the materials was confirmed determining the protein concentration in the soluble fraction after immobilization by the Bradford method. The optimal conditions of the biocatalyst activity were determined: oil / ethanol ratio, water percentage, amount of immobilized enzyme / mg of SBA-15 support, reaction time and activity of the biocatalyst respect to the metal impregnated in the solid used. Ca/SBA-15 material show the better activity as biocatalyst to biodiesel production using 400mg/g of lipase respect material, 1/4 oil/ethanol and 4 wt% of water respect oil. The transesterification reaction of triglycerides with ethanol for the production of biodiesel catalyzed by the LPF / SBA-15 / Ca biocatalyst has high batch yields, does not produce soap, uses low temperatures of 37 ° C and allows to separate the catalyst easily from the mixture.
Fil: Ferrero, Gabriel O. Consejo Nacional de Investigaciones Científicas y Técnica. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento Química General e Inorgánica; Argentina.
Fil: Sánchez Faba, Edgar M. Consejo Nacional de Investigaciones Científicas y Técnica. Universidad Tecnológica Nacional. Facultad Regional Córdoba. Centro de Investigación y Tecnología Química; Argentina.
Fil: Eimer, Griselda Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Tecnológica Nacional. Facultad Regional Córdoba. Centro de Investigación y Tecnología Química; Argentina.
Materia
Biodiesel
Enzymatic immobilization
Mesoporous material
SBA-15
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-05-27T21:52:17Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/6503
Acceder
id RIAUTN_482e1fee24b39d4343529f23f848b431
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network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel productionFerrero, Gabriel O.Sánchez Faba, Edgar M.Eimer, Griselda AlejandraBiodieselEnzymatic immobilizationMesoporous materialSBA-15The aim of this work is to design a catalyst for biofuels production from renewable raw materials by means of the immobilization of an enzyme on nano-structured solid supports [1,2]. In fact, the main purpose is to immobilize the lipase of Pseudomonas fluorescens in the pure SBA-15 (Santa Barbara Amorphous) mesoporous material [3] and to compare their activity in the biodiesel production using vegetable oils, with that of the enzyme immobilized on materials SBA-15 modified with metals (Ca, Na, Fe, Cu, Al, K)[4]. The physicochemical properties of the synthesized mesoporous materials were determined by Small-angle X-ray scattering (SAXS), Transmission electron microscopy (TEM) and UV-visible. The enzyme was immobilized by physical adsorption, mixing each mesoporous material with an enzyme solution. The effective incorporation of the enzyme in the materials was confirmed determining the protein concentration in the soluble fraction after immobilization by the Bradford method. The optimal conditions of the biocatalyst activity were determined: oil / ethanol ratio, water percentage, amount of immobilized enzyme / mg of SBA-15 support, reaction time and activity of the biocatalyst respect to the metal impregnated in the solid used. Ca/SBA-15 material show the better activity as biocatalyst to biodiesel production using 400mg/g of lipase respect material, 1/4 oil/ethanol and 4 wt% of water respect oil. The transesterification reaction of triglycerides with ethanol for the production of biodiesel catalyzed by the LPF / SBA-15 / Ca biocatalyst has high batch yields, does not produce soap, uses low temperatures of 37 ° C and allows to separate the catalyst easily from the mixture.Fil: Ferrero, Gabriel O. Consejo Nacional de Investigaciones Científicas y Técnica. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento Química General e Inorgánica; Argentina.Fil: Sánchez Faba, Edgar M. Consejo Nacional de Investigaciones Científicas y Técnica. Universidad Tecnológica Nacional. Facultad Regional Córdoba. Centro de Investigación y Tecnología Química; Argentina.Fil: Eimer, Griselda Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Tecnológica Nacional. Facultad Regional Córdoba. Centro de Investigación y Tecnología Química; Argentina.2022-05-27T21:52:17Z2022-05-27T21:52:17Z2017info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdf1° Energy, Efficiency and Environmental Sustainability Conference (2017).http://hdl.handle.net/20.500.12272/6503enginfo:eu-repo/semantics/openAccess2022-05-27T21:52:17Zhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 InternacionalEimer, Griselda AlejandraNo comercial. Sólo de uso académico.reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:45:59Zoai:ria.utn.edu.ar:20.500.12272/6503instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:45:59.806Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel production
title Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel production
spellingShingle Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel production
Ferrero, Gabriel O.
Biodiesel
Enzymatic immobilization
Mesoporous material
SBA-15
title_short Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel production
title_full Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel production
title_fullStr Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel production
title_full_unstemmed Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel production
title_sort Immobilization of pseudomonas fluorecens lipase in mesoporous materials to biodiesel production
dc.creator.none.fl_str_mv Ferrero, Gabriel O.
Sánchez Faba, Edgar M.
Eimer, Griselda Alejandra
author Ferrero, Gabriel O.
author_facet Ferrero, Gabriel O.
Sánchez Faba, Edgar M.
Eimer, Griselda Alejandra
author_role author
author2 Sánchez Faba, Edgar M.
Eimer, Griselda Alejandra
author2_role author
author
dc.subject.none.fl_str_mv Biodiesel
Enzymatic immobilization
Mesoporous material
SBA-15
topic Biodiesel
Enzymatic immobilization
Mesoporous material
SBA-15
dc.description.none.fl_txt_mv The aim of this work is to design a catalyst for biofuels production from renewable raw materials by means of the immobilization of an enzyme on nano-structured solid supports [1,2]. In fact, the main purpose is to immobilize the lipase of Pseudomonas fluorescens in the pure SBA-15 (Santa Barbara Amorphous) mesoporous material [3] and to compare their activity in the biodiesel production using vegetable oils, with that of the enzyme immobilized on materials SBA-15 modified with metals (Ca, Na, Fe, Cu, Al, K)[4]. The physicochemical properties of the synthesized mesoporous materials were determined by Small-angle X-ray scattering (SAXS), Transmission electron microscopy (TEM) and UV-visible. The enzyme was immobilized by physical adsorption, mixing each mesoporous material with an enzyme solution. The effective incorporation of the enzyme in the materials was confirmed determining the protein concentration in the soluble fraction after immobilization by the Bradford method. The optimal conditions of the biocatalyst activity were determined: oil / ethanol ratio, water percentage, amount of immobilized enzyme / mg of SBA-15 support, reaction time and activity of the biocatalyst respect to the metal impregnated in the solid used. Ca/SBA-15 material show the better activity as biocatalyst to biodiesel production using 400mg/g of lipase respect material, 1/4 oil/ethanol and 4 wt% of water respect oil. The transesterification reaction of triglycerides with ethanol for the production of biodiesel catalyzed by the LPF / SBA-15 / Ca biocatalyst has high batch yields, does not produce soap, uses low temperatures of 37 ° C and allows to separate the catalyst easily from the mixture.
Fil: Ferrero, Gabriel O. Consejo Nacional de Investigaciones Científicas y Técnica. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento Química General e Inorgánica; Argentina.
Fil: Sánchez Faba, Edgar M. Consejo Nacional de Investigaciones Científicas y Técnica. Universidad Tecnológica Nacional. Facultad Regional Córdoba. Centro de Investigación y Tecnología Química; Argentina.
Fil: Eimer, Griselda Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Tecnológica Nacional. Facultad Regional Córdoba. Centro de Investigación y Tecnología Química; Argentina.
description The aim of this work is to design a catalyst for biofuels production from renewable raw materials by means of the immobilization of an enzyme on nano-structured solid supports [1,2]. In fact, the main purpose is to immobilize the lipase of Pseudomonas fluorescens in the pure SBA-15 (Santa Barbara Amorphous) mesoporous material [3] and to compare their activity in the biodiesel production using vegetable oils, with that of the enzyme immobilized on materials SBA-15 modified with metals (Ca, Na, Fe, Cu, Al, K)[4]. The physicochemical properties of the synthesized mesoporous materials were determined by Small-angle X-ray scattering (SAXS), Transmission electron microscopy (TEM) and UV-visible. The enzyme was immobilized by physical adsorption, mixing each mesoporous material with an enzyme solution. The effective incorporation of the enzyme in the materials was confirmed determining the protein concentration in the soluble fraction after immobilization by the Bradford method. The optimal conditions of the biocatalyst activity were determined: oil / ethanol ratio, water percentage, amount of immobilized enzyme / mg of SBA-15 support, reaction time and activity of the biocatalyst respect to the metal impregnated in the solid used. Ca/SBA-15 material show the better activity as biocatalyst to biodiesel production using 400mg/g of lipase respect material, 1/4 oil/ethanol and 4 wt% of water respect oil. The transesterification reaction of triglycerides with ethanol for the production of biodiesel catalyzed by the LPF / SBA-15 / Ca biocatalyst has high batch yields, does not produce soap, uses low temperatures of 37 ° C and allows to separate the catalyst easily from the mixture.
publishDate 2017
dc.date.none.fl_str_mv 2017
2022-05-27T21:52:17Z
2022-05-27T21:52:17Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv 1° Energy, Efficiency and Environmental Sustainability Conference (2017).
http://hdl.handle.net/20.500.12272/6503
identifier_str_mv 1° Energy, Efficiency and Environmental Sustainability Conference (2017).
url http://hdl.handle.net/20.500.12272/6503
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-05-27T21:52:17Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Eimer, Griselda Alejandra
No comercial. Sólo de uso académico.
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-05-27T21:52:17Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Eimer, Griselda Alejandra
No comercial. Sólo de uso académico.
dc.format.none.fl_str_mv pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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