Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.

Autores
Conde Molina, Debora; Sanchez Hulmedilla, Celene; Silva, Fausto; Piperata, Gabriela
Año de publicación
2022
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión aceptada
Descripción
The environment is negatively impacted by occasional discharges from industrial activity. When these discharges contain insoluble compounds such as fats, oils and grease, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Campana, Buenos Aires, where a vegetable oil residues treatment industry generated a significant uncontrolled discharge of waste into Lagoon 3 of the private nature reserve El Morejón. In view of this, there is a need to address bioremediation strategies to clean up the area. The aim of this work is to study growth conditions of vegetable oil degrading bacterial consortia, previously isolated from lagoon 3, with a view to applying it as an inoculum in bioaugmentation strategy. For this purpose, bacteria were tested in flask containing liquid culture media at different conditions, such as: medium formulated with 2-5 % v/v vegetable oil -as only carbon source-, shaking at 135-220 rpm, medium formulated with alternative carbon source of sweet potato waste (5% w/v). The best condition for the growth of bacteria was a culture medium formulated with 5 % v/v vegetable oil, incubated at 135 rpm for days, reaching 9 g/L of biomass. Moreover, this condition maintains the selection pressure so that bacteria preserve the ability to degrade vegetable oils. The significant biomass obtained positions these autochthonous bacteria with great potential to be applied as bioaugmentation in site-specific bioremediation strategy for the remediation of Lagoon 3.
Fil: Conde Molina, Debora.Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Sanchez Hulmedilla, Celene. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Silva, Fausto. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Piperata, Gabriela. Termoeléctrica Manuel Belgrano; Argentina.
Materia
UTN
Facultad Regional Delta
Vegetable oil degrading bacteria
Biomass
Agro-industrial wastes as substrates
Bioaugmentation
Bioremediation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-12-29T20:57:49Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/7471
Acceder
id RIAUTN_94fea37177251be69f89ba975f55f945
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network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.Conde Molina, DeboraSanchez Hulmedilla, CeleneSilva, FaustoPiperata, GabrielaUTNFacultad Regional DeltaVegetable oil degrading bacteriaBiomassAgro-industrial wastes as substratesBioaugmentationBioremediationThe environment is negatively impacted by occasional discharges from industrial activity. When these discharges contain insoluble compounds such as fats, oils and grease, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Campana, Buenos Aires, where a vegetable oil residues treatment industry generated a significant uncontrolled discharge of waste into Lagoon 3 of the private nature reserve El Morejón. In view of this, there is a need to address bioremediation strategies to clean up the area. The aim of this work is to study growth conditions of vegetable oil degrading bacterial consortia, previously isolated from lagoon 3, with a view to applying it as an inoculum in bioaugmentation strategy. For this purpose, bacteria were tested in flask containing liquid culture media at different conditions, such as: medium formulated with 2-5 % v/v vegetable oil -as only carbon source-, shaking at 135-220 rpm, medium formulated with alternative carbon source of sweet potato waste (5% w/v). The best condition for the growth of bacteria was a culture medium formulated with 5 % v/v vegetable oil, incubated at 135 rpm for days, reaching 9 g/L of biomass. Moreover, this condition maintains the selection pressure so that bacteria preserve the ability to degrade vegetable oils. The significant biomass obtained positions these autochthonous bacteria with great potential to be applied as bioaugmentation in site-specific bioremediation strategy for the remediation of Lagoon 3.Fil: Conde Molina, Debora.Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.Fil: Sanchez Hulmedilla, Celene. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.Fil: Silva, Fausto. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.Fil: Piperata, Gabriela. Termoeléctrica Manuel Belgrano; Argentina.Encuentro Internacional de Ciencias de la Tierra2022-12-29T20:57:49Z2022-12-29T20:57:49Z2022-11info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfSánchez Holmedilla C., Silva F., Piperata G., Conde Molina D. Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites. 17° Encuentro Internacional de Ciencias de la Tierra. Mendoza, Argentinhttp://hdl.handle.net/20.500.12272/7471spainfo:eu-repo/semantics/openAccess2022-12-29T20:57:49Zhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 InternacionalLos autoresAtribución – No Comercial – Sin Obra Derivada (by-nc-nd)reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:44:45Zoai:ria.utn.edu.ar:20.500.12272/7471instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:44:47.188Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
title Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
spellingShingle Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
Conde Molina, Debora
UTN
Facultad Regional Delta
Vegetable oil degrading bacteria
Biomass
Agro-industrial wastes as substrates
Bioaugmentation
Bioremediation
title_short Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
title_full Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
title_fullStr Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
title_full_unstemmed Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
title_sort Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
dc.creator.none.fl_str_mv Conde Molina, Debora
Sanchez Hulmedilla, Celene
Silva, Fausto
Piperata, Gabriela
author Conde Molina, Debora
author_facet Conde Molina, Debora
Sanchez Hulmedilla, Celene
Silva, Fausto
Piperata, Gabriela
author_role author
author2 Sanchez Hulmedilla, Celene
Silva, Fausto
Piperata, Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv UTN
Facultad Regional Delta
Vegetable oil degrading bacteria
Biomass
Agro-industrial wastes as substrates
Bioaugmentation
Bioremediation
topic UTN
Facultad Regional Delta
Vegetable oil degrading bacteria
Biomass
Agro-industrial wastes as substrates
Bioaugmentation
Bioremediation
dc.description.none.fl_txt_mv The environment is negatively impacted by occasional discharges from industrial activity. When these discharges contain insoluble compounds such as fats, oils and grease, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Campana, Buenos Aires, where a vegetable oil residues treatment industry generated a significant uncontrolled discharge of waste into Lagoon 3 of the private nature reserve El Morejón. In view of this, there is a need to address bioremediation strategies to clean up the area. The aim of this work is to study growth conditions of vegetable oil degrading bacterial consortia, previously isolated from lagoon 3, with a view to applying it as an inoculum in bioaugmentation strategy. For this purpose, bacteria were tested in flask containing liquid culture media at different conditions, such as: medium formulated with 2-5 % v/v vegetable oil -as only carbon source-, shaking at 135-220 rpm, medium formulated with alternative carbon source of sweet potato waste (5% w/v). The best condition for the growth of bacteria was a culture medium formulated with 5 % v/v vegetable oil, incubated at 135 rpm for days, reaching 9 g/L of biomass. Moreover, this condition maintains the selection pressure so that bacteria preserve the ability to degrade vegetable oils. The significant biomass obtained positions these autochthonous bacteria with great potential to be applied as bioaugmentation in site-specific bioremediation strategy for the remediation of Lagoon 3.
Fil: Conde Molina, Debora.Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Sanchez Hulmedilla, Celene. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Silva, Fausto. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Piperata, Gabriela. Termoeléctrica Manuel Belgrano; Argentina.
description The environment is negatively impacted by occasional discharges from industrial activity. When these discharges contain insoluble compounds such as fats, oils and grease, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Campana, Buenos Aires, where a vegetable oil residues treatment industry generated a significant uncontrolled discharge of waste into Lagoon 3 of the private nature reserve El Morejón. In view of this, there is a need to address bioremediation strategies to clean up the area. The aim of this work is to study growth conditions of vegetable oil degrading bacterial consortia, previously isolated from lagoon 3, with a view to applying it as an inoculum in bioaugmentation strategy. For this purpose, bacteria were tested in flask containing liquid culture media at different conditions, such as: medium formulated with 2-5 % v/v vegetable oil -as only carbon source-, shaking at 135-220 rpm, medium formulated with alternative carbon source of sweet potato waste (5% w/v). The best condition for the growth of bacteria was a culture medium formulated with 5 % v/v vegetable oil, incubated at 135 rpm for days, reaching 9 g/L of biomass. Moreover, this condition maintains the selection pressure so that bacteria preserve the ability to degrade vegetable oils. The significant biomass obtained positions these autochthonous bacteria with great potential to be applied as bioaugmentation in site-specific bioremediation strategy for the remediation of Lagoon 3.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-29T20:57:49Z
2022-12-29T20:57:49Z
2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str acceptedVersion
dc.identifier.none.fl_str_mv Sánchez Holmedilla C., Silva F., Piperata G., Conde Molina D. Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites. 17° Encuentro Internacional de Ciencias de la Tierra. Mendoza, Argentin
http://hdl.handle.net/20.500.12272/7471
identifier_str_mv Sánchez Holmedilla C., Silva F., Piperata G., Conde Molina D. Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites. 17° Encuentro Internacional de Ciencias de la Tierra. Mendoza, Argentin
url http://hdl.handle.net/20.500.12272/7471
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-12-29T20:57:49Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-12-29T20:57:49Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Encuentro Internacional de Ciencias de la Tierra
publisher.none.fl_str_mv Encuentro Internacional de Ciencias de la Tierra
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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score 13.265058

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