Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.

Autores
Conde Molina, Debora; Liporace, Franco; Quevedo, Carla
Año de publicación
2022
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
These days, petroleum hydrocarbon pollution has become a global problem, because of this, bioremediation is presented as a strategy for cleaning up sites contaminated with organic pollutants, and it has an increasing role in relation to the potential it presents as a non-invasive and cost-effective technology. The aim of this study is to optimize the biomass production of Pseudomonas sp. MT1A3 strain as a soil bioremediation approach for petroleum hydrocarbon polluted environments. Factorial experimental designs were employed to study the effect of several factors of composition medium and incubation conditions on biomass production. Agro-industrial wastes such as peanut oil as carbon source, NaNO3 as nitrogen source and incubation temperature were found to be significant independent variables. These factors were further optimized using Box–Behnken design. Combination of peanut oil 18.69 g/L, NaNO3 2.39 g/L and 26.06 °C incubation temperature was optimum for maximum biomass production of MT1A3 and the model validated in a bioreactor allowed to obtain 9.67g/L. Based on these results, this autochthonous strain was applied in bioaugmentation as a bioremediation strategy through microcosm designs, reaching 93.52% of total hydrocarbon removal at 60 days. This constitutes a promising alternative for hydrocarbon-contaminated soil.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Liporace, Franco. Universidad Tecnologica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Quevedo, Carla. Universidad Tecnologica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Peer Reviewed
Materia
UTN
Facultad Regional Delta
Hydrocarbon-contaminated soil
Bioremediation
Optimization
Biomass
Pseudomonas sp.
Microcosms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-12-02T19:23:37Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/7350
Acceder
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network_acronym_str RIAUTN
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network_name_str Repositorio Institucional Abierto (UTN)
spelling Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.Conde Molina, DeboraLiporace, FrancoQuevedo, CarlaUTNFacultad Regional DeltaHydrocarbon-contaminated soilBioremediationOptimizationBiomassPseudomonas sp.MicrocosmsThese days, petroleum hydrocarbon pollution has become a global problem, because of this, bioremediation is presented as a strategy for cleaning up sites contaminated with organic pollutants, and it has an increasing role in relation to the potential it presents as a non-invasive and cost-effective technology. The aim of this study is to optimize the biomass production of Pseudomonas sp. MT1A3 strain as a soil bioremediation approach for petroleum hydrocarbon polluted environments. Factorial experimental designs were employed to study the effect of several factors of composition medium and incubation conditions on biomass production. Agro-industrial wastes such as peanut oil as carbon source, NaNO3 as nitrogen source and incubation temperature were found to be significant independent variables. These factors were further optimized using Box–Behnken design. Combination of peanut oil 18.69 g/L, NaNO3 2.39 g/L and 26.06 °C incubation temperature was optimum for maximum biomass production of MT1A3 and the model validated in a bioreactor allowed to obtain 9.67g/L. Based on these results, this autochthonous strain was applied in bioaugmentation as a bioremediation strategy through microcosm designs, reaching 93.52% of total hydrocarbon removal at 60 days. This constitutes a promising alternative for hydrocarbon-contaminated soil.Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Fil: Liporace, Franco. Universidad Tecnologica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Fil: Quevedo, Carla. Universidad Tecnologica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Peer Reviewed2022-12-02T19:23:37Z2022-12-02T19:23:37Z2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articulopdfapplication/pdfConde Molina D., Liporace F., Quevedo C. Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil. Biotech 3, 12, 11http://hdl.handle.net/20.500.12272/735010.1007spainfo:eu-repo/semantics/openAccess2022-12-02T19:23:37Zhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 InternacionalLos autoresAtribución – No Comercial – Sin Obra Derivada (by-nc-nd)reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-10-01T11:58:50Zoai:ria.utn.edu.ar:20.500.12272/7350instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-10-01 11:58:52.283Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
title Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
spellingShingle Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
Conde Molina, Debora
UTN
Facultad Regional Delta
Hydrocarbon-contaminated soil
Bioremediation
Optimization
Biomass
Pseudomonas sp.
Microcosms
title_short Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
title_full Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
title_fullStr Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
title_full_unstemmed Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
title_sort Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
dc.creator.none.fl_str_mv Conde Molina, Debora
Liporace, Franco
Quevedo, Carla
author Conde Molina, Debora
author_facet Conde Molina, Debora
Liporace, Franco
Quevedo, Carla
author_role author
author2 Liporace, Franco
Quevedo, Carla
author2_role author
author
dc.subject.none.fl_str_mv UTN
Facultad Regional Delta
Hydrocarbon-contaminated soil
Bioremediation
Optimization
Biomass
Pseudomonas sp.
Microcosms
topic UTN
Facultad Regional Delta
Hydrocarbon-contaminated soil
Bioremediation
Optimization
Biomass
Pseudomonas sp.
Microcosms
dc.description.none.fl_txt_mv These days, petroleum hydrocarbon pollution has become a global problem, because of this, bioremediation is presented as a strategy for cleaning up sites contaminated with organic pollutants, and it has an increasing role in relation to the potential it presents as a non-invasive and cost-effective technology. The aim of this study is to optimize the biomass production of Pseudomonas sp. MT1A3 strain as a soil bioremediation approach for petroleum hydrocarbon polluted environments. Factorial experimental designs were employed to study the effect of several factors of composition medium and incubation conditions on biomass production. Agro-industrial wastes such as peanut oil as carbon source, NaNO3 as nitrogen source and incubation temperature were found to be significant independent variables. These factors were further optimized using Box–Behnken design. Combination of peanut oil 18.69 g/L, NaNO3 2.39 g/L and 26.06 °C incubation temperature was optimum for maximum biomass production of MT1A3 and the model validated in a bioreactor allowed to obtain 9.67g/L. Based on these results, this autochthonous strain was applied in bioaugmentation as a bioremediation strategy through microcosm designs, reaching 93.52% of total hydrocarbon removal at 60 days. This constitutes a promising alternative for hydrocarbon-contaminated soil.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Liporace, Franco. Universidad Tecnologica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Quevedo, Carla. Universidad Tecnologica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Peer Reviewed
description These days, petroleum hydrocarbon pollution has become a global problem, because of this, bioremediation is presented as a strategy for cleaning up sites contaminated with organic pollutants, and it has an increasing role in relation to the potential it presents as a non-invasive and cost-effective technology. The aim of this study is to optimize the biomass production of Pseudomonas sp. MT1A3 strain as a soil bioremediation approach for petroleum hydrocarbon polluted environments. Factorial experimental designs were employed to study the effect of several factors of composition medium and incubation conditions on biomass production. Agro-industrial wastes such as peanut oil as carbon source, NaNO3 as nitrogen source and incubation temperature were found to be significant independent variables. These factors were further optimized using Box–Behnken design. Combination of peanut oil 18.69 g/L, NaNO3 2.39 g/L and 26.06 °C incubation temperature was optimum for maximum biomass production of MT1A3 and the model validated in a bioreactor allowed to obtain 9.67g/L. Based on these results, this autochthonous strain was applied in bioaugmentation as a bioremediation strategy through microcosm designs, reaching 93.52% of total hydrocarbon removal at 60 days. This constitutes a promising alternative for hydrocarbon-contaminated soil.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-02T19:23:37Z
2022-12-02T19:23:37Z
2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Conde Molina D., Liporace F., Quevedo C. Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil. Biotech 3, 12, 11
http://hdl.handle.net/20.500.12272/7350
10.1007
identifier_str_mv Conde Molina D., Liporace F., Quevedo C. Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil. Biotech 3, 12, 11
10.1007
url http://hdl.handle.net/20.500.12272/7350
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-12-02T19:23:37Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-12-02T19:23:37Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
dc.format.none.fl_str_mv pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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