Furfural removal from liquid systems by antinobacteria

Autores
Echeverría, Macarena Celeste; Benimeli, Claudia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Many industries such as petrochemical, pulp and paper, pharmaceutical, and food industries involve processes that use or produce furfural. Furfural is a heterocyclic aldehyde obtained by dehydrating at high temperatures of xylose; therefore, it is a characteristic compound present in acid hydrolyzates in which the furfural concentration can usually reach 2–3 g/l. In the region Northeast of Argentina (NEA), furfural is produced from detanized quebracho sawdust. In NEA, wastewaters derived from furfural production contain around 800 mg/1 of this compound, which can cause toxic effects on living systems if they are released into the environment without proper treatment. In the present work, the removal of different concentrations of furfural by actinobacteria from liquid systems was studied. Isolates of actinobacteria called L4, L6, L9 and L13 obtained from sediments of stabilization ponds of a furfural-producing plant in the NEA region, and Streptomyces sp. A5, A6, A12 and M7, obtained from sites contaminated with other xenobiotic compounds, were selected on base of their tolerance to furfural in Starch Casein Agar medium. In order to select the most efficient actinobacteria with respect to their growth and furfural removal ability in liquid medium, Minimal Medium (MM) added with a furfural concentration of 418±1 mg/1 as the only carbon and energy source was used. This selection was carried out by determining the minimum relationship between the concentration of residual furfural and the microbial growth. Streptomyces sp. A12 and M7 and strain L9 were selected because they showed the minimal relationship. Subsequently, the selected strains, as pure and mixed cultures, were inoculated in MM supplemented with furfural 807±10 mg/1 as the only carbon and energy source. The results showed that the three pure cultures were able to grow and develop under these conditions; however, the culture for which the relationship mentioned above was minimal, was the consortium formed by the actinobacteria L9, A12 and M7. In order to evaluate the effectiveness of the bioremediation process, ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). The culture supernatants were evaluated before and after its treatment for each condition. In response, inhibition of germination and elongation of the radicle and hypocotyl were determined in the presence of furfural. Significant increases in these bioindicators (p < 0.05) were obtained when the treatment was carried out with the consortium formed by the actinobacteria L9, A12 and M7. The results obtained suggest that the selected actinobacteria consortium represents a promising bioremediation tool for the treatment of effluents containing furfural.
Fil: Echeverría, Macarena Celeste. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos (GISTAQ); Argentina.
Fil: Benimeli, Claudia. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina. Fil: Benimeli, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Peer Reviewed
Materia
Furfural
Actinobacteria
Bioremediation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2023-07-10T22:36:57Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/8251
Acceder
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network_acronym_str RIAUTN
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spelling Furfural removal from liquid systems by antinobacteriaEcheverría, Macarena CelesteBenimeli, ClaudiaFurfuralActinobacteriaBioremediationMany industries such as petrochemical, pulp and paper, pharmaceutical, and food industries involve processes that use or produce furfural. Furfural is a heterocyclic aldehyde obtained by dehydrating at high temperatures of xylose; therefore, it is a characteristic compound present in acid hydrolyzates in which the furfural concentration can usually reach 2–3 g/l. In the region Northeast of Argentina (NEA), furfural is produced from detanized quebracho sawdust. In NEA, wastewaters derived from furfural production contain around 800 mg/1 of this compound, which can cause toxic effects on living systems if they are released into the environment without proper treatment. In the present work, the removal of different concentrations of furfural by actinobacteria from liquid systems was studied. Isolates of actinobacteria called L4, L6, L9 and L13 obtained from sediments of stabilization ponds of a furfural-producing plant in the NEA region, and Streptomyces sp. A5, A6, A12 and M7, obtained from sites contaminated with other xenobiotic compounds, were selected on base of their tolerance to furfural in Starch Casein Agar medium. In order to select the most efficient actinobacteria with respect to their growth and furfural removal ability in liquid medium, Minimal Medium (MM) added with a furfural concentration of 418±1 mg/1 as the only carbon and energy source was used. This selection was carried out by determining the minimum relationship between the concentration of residual furfural and the microbial growth. Streptomyces sp. A12 and M7 and strain L9 were selected because they showed the minimal relationship. Subsequently, the selected strains, as pure and mixed cultures, were inoculated in MM supplemented with furfural 807±10 mg/1 as the only carbon and energy source. The results showed that the three pure cultures were able to grow and develop under these conditions; however, the culture for which the relationship mentioned above was minimal, was the consortium formed by the actinobacteria L9, A12 and M7. In order to evaluate the effectiveness of the bioremediation process, ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). The culture supernatants were evaluated before and after its treatment for each condition. In response, inhibition of germination and elongation of the radicle and hypocotyl were determined in the presence of furfural. Significant increases in these bioindicators (p &lt; 0.05) were obtained when the treatment was carried out with the consortium formed by the actinobacteria L9, A12 and M7. The results obtained suggest that the selected actinobacteria consortium represents a promising bioremediation tool for the treatment of effluents containing furfural.Fil: Echeverría, Macarena Celeste. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos (GISTAQ); Argentina.Fil: Benimeli, Claudia. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina. Fil: Benimeli, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaPeer Reviewed2023-07-10T22:36:57Z2023-07-10T22:36:57Z2022-10-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articulopdfapplication/pdf1666-7948http://hdl.handle.net/20.500.12272/8251enginfo:eu-repo/semantics/openAccess2023-07-10T22:36:57ZAcceso Abiertoreponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:48:34Zoai:ria.utn.edu.ar:20.500.12272/8251instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:48:35.387Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Furfural removal from liquid systems by antinobacteria
title Furfural removal from liquid systems by antinobacteria
spellingShingle Furfural removal from liquid systems by antinobacteria
Echeverría, Macarena Celeste
Furfural
Actinobacteria
Bioremediation
title_short Furfural removal from liquid systems by antinobacteria
title_full Furfural removal from liquid systems by antinobacteria
title_fullStr Furfural removal from liquid systems by antinobacteria
title_full_unstemmed Furfural removal from liquid systems by antinobacteria
title_sort Furfural removal from liquid systems by antinobacteria
dc.creator.none.fl_str_mv Echeverría, Macarena Celeste
Benimeli, Claudia
author Echeverría, Macarena Celeste
author_facet Echeverría, Macarena Celeste
Benimeli, Claudia
author_role author
author2 Benimeli, Claudia
author2_role author
dc.subject.none.fl_str_mv Furfural
Actinobacteria
Bioremediation
topic Furfural
Actinobacteria
Bioremediation
dc.description.none.fl_txt_mv Many industries such as petrochemical, pulp and paper, pharmaceutical, and food industries involve processes that use or produce furfural. Furfural is a heterocyclic aldehyde obtained by dehydrating at high temperatures of xylose; therefore, it is a characteristic compound present in acid hydrolyzates in which the furfural concentration can usually reach 2–3 g/l. In the region Northeast of Argentina (NEA), furfural is produced from detanized quebracho sawdust. In NEA, wastewaters derived from furfural production contain around 800 mg/1 of this compound, which can cause toxic effects on living systems if they are released into the environment without proper treatment. In the present work, the removal of different concentrations of furfural by actinobacteria from liquid systems was studied. Isolates of actinobacteria called L4, L6, L9 and L13 obtained from sediments of stabilization ponds of a furfural-producing plant in the NEA region, and Streptomyces sp. A5, A6, A12 and M7, obtained from sites contaminated with other xenobiotic compounds, were selected on base of their tolerance to furfural in Starch Casein Agar medium. In order to select the most efficient actinobacteria with respect to their growth and furfural removal ability in liquid medium, Minimal Medium (MM) added with a furfural concentration of 418±1 mg/1 as the only carbon and energy source was used. This selection was carried out by determining the minimum relationship between the concentration of residual furfural and the microbial growth. Streptomyces sp. A12 and M7 and strain L9 were selected because they showed the minimal relationship. Subsequently, the selected strains, as pure and mixed cultures, were inoculated in MM supplemented with furfural 807±10 mg/1 as the only carbon and energy source. The results showed that the three pure cultures were able to grow and develop under these conditions; however, the culture for which the relationship mentioned above was minimal, was the consortium formed by the actinobacteria L9, A12 and M7. In order to evaluate the effectiveness of the bioremediation process, ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). The culture supernatants were evaluated before and after its treatment for each condition. In response, inhibition of germination and elongation of the radicle and hypocotyl were determined in the presence of furfural. Significant increases in these bioindicators (p &lt; 0.05) were obtained when the treatment was carried out with the consortium formed by the actinobacteria L9, A12 and M7. The results obtained suggest that the selected actinobacteria consortium represents a promising bioremediation tool for the treatment of effluents containing furfural.
Fil: Echeverría, Macarena Celeste. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos (GISTAQ); Argentina.
Fil: Benimeli, Claudia. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina. Fil: Benimeli, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Peer Reviewed
description Many industries such as petrochemical, pulp and paper, pharmaceutical, and food industries involve processes that use or produce furfural. Furfural is a heterocyclic aldehyde obtained by dehydrating at high temperatures of xylose; therefore, it is a characteristic compound present in acid hydrolyzates in which the furfural concentration can usually reach 2–3 g/l. In the region Northeast of Argentina (NEA), furfural is produced from detanized quebracho sawdust. In NEA, wastewaters derived from furfural production contain around 800 mg/1 of this compound, which can cause toxic effects on living systems if they are released into the environment without proper treatment. In the present work, the removal of different concentrations of furfural by actinobacteria from liquid systems was studied. Isolates of actinobacteria called L4, L6, L9 and L13 obtained from sediments of stabilization ponds of a furfural-producing plant in the NEA region, and Streptomyces sp. A5, A6, A12 and M7, obtained from sites contaminated with other xenobiotic compounds, were selected on base of their tolerance to furfural in Starch Casein Agar medium. In order to select the most efficient actinobacteria with respect to their growth and furfural removal ability in liquid medium, Minimal Medium (MM) added with a furfural concentration of 418±1 mg/1 as the only carbon and energy source was used. This selection was carried out by determining the minimum relationship between the concentration of residual furfural and the microbial growth. Streptomyces sp. A12 and M7 and strain L9 were selected because they showed the minimal relationship. Subsequently, the selected strains, as pure and mixed cultures, were inoculated in MM supplemented with furfural 807±10 mg/1 as the only carbon and energy source. The results showed that the three pure cultures were able to grow and develop under these conditions; however, the culture for which the relationship mentioned above was minimal, was the consortium formed by the actinobacteria L9, A12 and M7. In order to evaluate the effectiveness of the bioremediation process, ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). The culture supernatants were evaluated before and after its treatment for each condition. In response, inhibition of germination and elongation of the radicle and hypocotyl were determined in the presence of furfural. Significant increases in these bioindicators (p &lt; 0.05) were obtained when the treatment was carried out with the consortium formed by the actinobacteria L9, A12 and M7. The results obtained suggest that the selected actinobacteria consortium represents a promising bioremediation tool for the treatment of effluents containing furfural.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-25
2023-07-10T22:36:57Z
2023-07-10T22:36:57Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1666-7948
http://hdl.handle.net/20.500.12272/8251
identifier_str_mv 1666-7948
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dc.language.none.fl_str_mv eng
language eng
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2023-07-10T22:36:57Z
Acceso Abierto
eu_rights_str_mv openAccess
rights_invalid_str_mv 2023-07-10T22:36:57Z
Acceso Abierto
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reponame_str Repositorio Institucional Abierto (UTN)
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instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
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