Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish model

Autores
Vercellini, María Clara; Carranza Martin, Ana Cristina; Montes, Martín Miguel; Ayala, Mauro Alejandro
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Vercellini, María Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Carranza-Martín, Ana. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Montes, Martín Miguel. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ayala, Mauro Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General; Argentina
Anesthetic agents with antiparasitic properties offer dual advantages in aquaculture and experimental research by facilitating fish handling and potentially reducing parasite mean abundance (MA) and prevalence (P). Eugenol and benzocaine are commonly used anesthetics, yet their combined anesthetic and antiparasitic effects remain underexplored, especially in Neotropical species. This study assessed these effects in Cnesterodon decemmaculatus, a sentinel species in ecological and toxicological studies. In November 2023 and February 2024, 251 specimens were collected from a lake in La Plata, Argentina, and exposed to eugenol (10, 20, 30, 40, 50 mg L⁻1) and benzocaine (20, 50, 80, 100, 150 mg L⁻1), with the controls groups. Induction and recovery times were recorded. Parasite indices MA and P were calculated for Echinostomatidae (gills), Ascocotyle sp. (heart, mesentery), Saccocoelioides kirchneri (intestine), and Trichodina sp. (tegument). Echinostomatidae MA was analyzed via log-transformed linear models, while other taxa were assessed with generalized linear models, including sex, total length, standard length, and weight as covariates. Results demonstrated that eugenol 50 mg L⁻1 induced anesthesia more rapidly with increasing concentrations, whereas recovery time remained unaffected. Benzocaine yielded significantly faster recovery at 100 mg L⁻1, with SL significantly influencing induction time. Eugenol at 50 mg L⁻1 significantly decreased Echinostomatidae MA (P = 0.05), while benzocaine significantly reduced Trichodina sp. prevalence (P < 0.001). Parasite MA and P were modulated by sex and morphometric variables. These findings underscore the dual utility of eugenol and benzocaine as anesthetic and antiparasitic agents within experimental protocols targeting fish handling, welfare, and parasite management.
Fuente
Aquaculture International, vol. 33, n. 6, art. 400
Materia
ANESTESICOS
ANTIPARASITARIOS
ACUICULTURA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/21965
Acceder
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oai_identifier_str oai:ucacris:123456789/21965
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish modelVercellini, María ClaraCarranza Martin, Ana CristinaMontes, Martín MiguelAyala, Mauro AlejandroANESTESICOSANTIPARASITARIOSACUICULTURAFil: Vercellini, María Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Carranza-Martín, Ana. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaFil: Montes, Martín Miguel. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Ayala, Mauro Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General; ArgentinaAnesthetic agents with antiparasitic properties offer dual advantages in aquaculture and experimental research by facilitating fish handling and potentially reducing parasite mean abundance (MA) and prevalence (P). Eugenol and benzocaine are commonly used anesthetics, yet their combined anesthetic and antiparasitic effects remain underexplored, especially in Neotropical species. This study assessed these effects in Cnesterodon decemmaculatus, a sentinel species in ecological and toxicological studies. In November 2023 and February 2024, 251 specimens were collected from a lake in La Plata, Argentina, and exposed to eugenol (10, 20, 30, 40, 50 mg L⁻1) and benzocaine (20, 50, 80, 100, 150 mg L⁻1), with the controls groups. Induction and recovery times were recorded. Parasite indices MA and P were calculated for Echinostomatidae (gills), Ascocotyle sp. (heart, mesentery), Saccocoelioides kirchneri (intestine), and Trichodina sp. (tegument). Echinostomatidae MA was analyzed via log-transformed linear models, while other taxa were assessed with generalized linear models, including sex, total length, standard length, and weight as covariates. Results demonstrated that eugenol 50 mg L⁻1 induced anesthesia more rapidly with increasing concentrations, whereas recovery time remained unaffected. Benzocaine yielded significantly faster recovery at 100 mg L⁻1, with SL significantly influencing induction time. Eugenol at 50 mg L⁻1 significantly decreased Echinostomatidae MA (P = 0.05), while benzocaine significantly reduced Trichodina sp. prevalence (P < 0.001). Parasite MA and P were modulated by sex and morphometric variables. These findings underscore the dual utility of eugenol and benzocaine as anesthetic and antiparasitic agents within experimental protocols targeting fish handling, welfare, and parasite management.Springer Nature2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/219651573-143X10.1007/s10499-025-02071-zAquaculture International, vol. 33, n. 6, art. 400reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2026-11-05T12:34:22Zoai:ucacris:123456789/21965instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852026-11-05 12:34:22.251Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish model
title Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish model
spellingShingle Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish model
Vercellini, María Clara
ANESTESICOS
ANTIPARASITARIOS
ACUICULTURA
title_short Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish model
title_full Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish model
title_fullStr Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish model
title_full_unstemmed Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish model
title_sort Evaluation of benzocaine and eugenol as anesthetics and antiparasitic agents in a freshwater fish model
dc.creator.none.fl_str_mv Vercellini, María Clara
Carranza Martin, Ana Cristina
Montes, Martín Miguel
Ayala, Mauro Alejandro
author Vercellini, María Clara
author_facet Vercellini, María Clara
Carranza Martin, Ana Cristina
Montes, Martín Miguel
Ayala, Mauro Alejandro
author_role author
author2 Carranza Martin, Ana Cristina
Montes, Martín Miguel
Ayala, Mauro Alejandro
author2_role author
author
author
dc.subject.none.fl_str_mv ANESTESICOS
ANTIPARASITARIOS
ACUICULTURA
topic ANESTESICOS
ANTIPARASITARIOS
ACUICULTURA
dc.description.none.fl_txt_mv Fil: Vercellini, María Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Carranza-Martín, Ana. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Montes, Martín Miguel. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ayala, Mauro Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General; Argentina
Anesthetic agents with antiparasitic properties offer dual advantages in aquaculture and experimental research by facilitating fish handling and potentially reducing parasite mean abundance (MA) and prevalence (P). Eugenol and benzocaine are commonly used anesthetics, yet their combined anesthetic and antiparasitic effects remain underexplored, especially in Neotropical species. This study assessed these effects in Cnesterodon decemmaculatus, a sentinel species in ecological and toxicological studies. In November 2023 and February 2024, 251 specimens were collected from a lake in La Plata, Argentina, and exposed to eugenol (10, 20, 30, 40, 50 mg L⁻1) and benzocaine (20, 50, 80, 100, 150 mg L⁻1), with the controls groups. Induction and recovery times were recorded. Parasite indices MA and P were calculated for Echinostomatidae (gills), Ascocotyle sp. (heart, mesentery), Saccocoelioides kirchneri (intestine), and Trichodina sp. (tegument). Echinostomatidae MA was analyzed via log-transformed linear models, while other taxa were assessed with generalized linear models, including sex, total length, standard length, and weight as covariates. Results demonstrated that eugenol 50 mg L⁻1 induced anesthesia more rapidly with increasing concentrations, whereas recovery time remained unaffected. Benzocaine yielded significantly faster recovery at 100 mg L⁻1, with SL significantly influencing induction time. Eugenol at 50 mg L⁻1 significantly decreased Echinostomatidae MA (P = 0.05), while benzocaine significantly reduced Trichodina sp. prevalence (P < 0.001). Parasite MA and P were modulated by sex and morphometric variables. These findings underscore the dual utility of eugenol and benzocaine as anesthetic and antiparasitic agents within experimental protocols targeting fish handling, welfare, and parasite management.
description Fil: Vercellini, María Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/21965
1573-143X
10.1007/s10499-025-02071-z
url https://repositorio.uca.edu.ar/handle/123456789/21965
identifier_str_mv 1573-143X
10.1007/s10499-025-02071-z
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Aquaculture International, vol. 33, n. 6, art. 400
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 12.823554

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