Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcome

Autores
Senna, Camila Agustina; Mul Fedele, Malena Lis; Aiello, Ignacio; Hokama, Guido; Golombek, Diego A.; Paladino, Natalia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Senna, Camila Agustina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Mul Fedele, Malena Lis. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Cronofisiología; Argentina
Fil: Aiello, Ignacio. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Hokama, Guido. Universidad Nacional de Quilmes. Laboratorio de Cronobiología; Argentina
Fil: Golombek, Diego A. Universidad de San Andrés. Laboratorio Interdisciplinario del Tiempo (LITERA); Argentina
Fil: Paladino, Natalia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Sepsis is a syndrome caused by a dysregulated host response to pathogens, representing the leading cause of death from infection. Various murine models of sepsis have shown a time-dependent response based on the time of induction. Mice stimulated with high doses of bacterial lipopolysaccharide (LPS) at the end of the day exhibit a higher mortality rate (~80%) compared with those inoculated in the middle of the night (~30%). In this work, we assessed the differences in serum proteins of septic mice during the day and night. Through this proteomic study, we found significant variations in metabolic pathways, including glucose metabolism, which were associated with a better prognosis. Therefore, we studied the glucose response to LPS during the day and night. In this context, we found an early peak of LPS-induced glucose exclusively at the time of worse prognosis. We also observed a hypoglycemic response to LPS, which was independent of the time of sepsis induction. Finally, we performed a set of metabolic manipulations to study how hyperglycemia influences sepsis severity in mice. We observed that suppressing the glucose peak during the day, through metformin administration, reduced sepsis severity. In contrast, nocturnal glucose administration with LPS was rapidly metabolized and also decreased sepsis severity. In conclusion, sepsis severity may be influenced by the metabolic state at the time of the stimulus. Metabolic rhythms could lead to differences in early glucose management, affecting the outcome of this pathology.
Fuente
Journal of Biological Rhythms, 2025
Materia
SEPSIS
RITMOS CIRCADIANOS
GLUCOSA
BIOMEDICINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/21938
Acceder
id RIUCA_aae1760efc326eb935a43938a87c2aab
oai_identifier_str oai:ucacris:123456789/21938
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcomeSenna, Camila AgustinaMul Fedele, Malena LisAiello, IgnacioHokama, GuidoGolombek, Diego A.Paladino, NataliaSEPSISRITMOS CIRCADIANOSGLUCOSABIOMEDICINAFil: Senna, Camila Agustina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Mul Fedele, Malena Lis. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Cronofisiología; ArgentinaFil: Aiello, Ignacio. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Hokama, Guido. Universidad Nacional de Quilmes. Laboratorio de Cronobiología; ArgentinaFil: Golombek, Diego A. Universidad de San Andrés. Laboratorio Interdisciplinario del Tiempo (LITERA); ArgentinaFil: Paladino, Natalia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaSepsis is a syndrome caused by a dysregulated host response to pathogens, representing the leading cause of death from infection. Various murine models of sepsis have shown a time-dependent response based on the time of induction. Mice stimulated with high doses of bacterial lipopolysaccharide (LPS) at the end of the day exhibit a higher mortality rate (~80%) compared with those inoculated in the middle of the night (~30%). In this work, we assessed the differences in serum proteins of septic mice during the day and night. Through this proteomic study, we found significant variations in metabolic pathways, including glucose metabolism, which were associated with a better prognosis. Therefore, we studied the glucose response to LPS during the day and night. In this context, we found an early peak of LPS-induced glucose exclusively at the time of worse prognosis. We also observed a hypoglycemic response to LPS, which was independent of the time of sepsis induction. Finally, we performed a set of metabolic manipulations to study how hyperglycemia influences sepsis severity in mice. We observed that suppressing the glucose peak during the day, through metformin administration, reduced sepsis severity. In contrast, nocturnal glucose administration with LPS was rapidly metabolized and also decreased sepsis severity. In conclusion, sepsis severity may be influenced by the metabolic state at the time of the stimulus. Metabolic rhythms could lead to differences in early glucose management, affecting the outcome of this pathology.SAGE Publications2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/219380748-730410.1177/07487304251378607Journal of Biological Rhythms, 2025reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2026-11-05T12:34:21Zoai:ucacris:123456789/21938instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852026-11-05 12:34:21.512Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcome
title Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcome
spellingShingle Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcome
Senna, Camila Agustina
SEPSIS
RITMOS CIRCADIANOS
GLUCOSA
BIOMEDICINA
title_short Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcome
title_full Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcome
title_fullStr Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcome
title_full_unstemmed Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcome
title_sort Time-dependent metabolic response in sepsis severity: the influence of glucose in the disease outcome
dc.creator.none.fl_str_mv Senna, Camila Agustina
Mul Fedele, Malena Lis
Aiello, Ignacio
Hokama, Guido
Golombek, Diego A.
Paladino, Natalia
author Senna, Camila Agustina
author_facet Senna, Camila Agustina
Mul Fedele, Malena Lis
Aiello, Ignacio
Hokama, Guido
Golombek, Diego A.
Paladino, Natalia
author_role author
author2 Mul Fedele, Malena Lis
Aiello, Ignacio
Hokama, Guido
Golombek, Diego A.
Paladino, Natalia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SEPSIS
RITMOS CIRCADIANOS
GLUCOSA
BIOMEDICINA
topic SEPSIS
RITMOS CIRCADIANOS
GLUCOSA
BIOMEDICINA
dc.description.none.fl_txt_mv Fil: Senna, Camila Agustina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Mul Fedele, Malena Lis. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Cronofisiología; Argentina
Fil: Aiello, Ignacio. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Hokama, Guido. Universidad Nacional de Quilmes. Laboratorio de Cronobiología; Argentina
Fil: Golombek, Diego A. Universidad de San Andrés. Laboratorio Interdisciplinario del Tiempo (LITERA); Argentina
Fil: Paladino, Natalia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Sepsis is a syndrome caused by a dysregulated host response to pathogens, representing the leading cause of death from infection. Various murine models of sepsis have shown a time-dependent response based on the time of induction. Mice stimulated with high doses of bacterial lipopolysaccharide (LPS) at the end of the day exhibit a higher mortality rate (~80%) compared with those inoculated in the middle of the night (~30%). In this work, we assessed the differences in serum proteins of septic mice during the day and night. Through this proteomic study, we found significant variations in metabolic pathways, including glucose metabolism, which were associated with a better prognosis. Therefore, we studied the glucose response to LPS during the day and night. In this context, we found an early peak of LPS-induced glucose exclusively at the time of worse prognosis. We also observed a hypoglycemic response to LPS, which was independent of the time of sepsis induction. Finally, we performed a set of metabolic manipulations to study how hyperglycemia influences sepsis severity in mice. We observed that suppressing the glucose peak during the day, through metformin administration, reduced sepsis severity. In contrast, nocturnal glucose administration with LPS was rapidly metabolized and also decreased sepsis severity. In conclusion, sepsis severity may be influenced by the metabolic state at the time of the stimulus. Metabolic rhythms could lead to differences in early glucose management, affecting the outcome of this pathology.
description Fil: Senna, Camila Agustina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/21938
0748-7304
10.1177/07487304251378607
url https://repositorio.uca.edu.ar/handle/123456789/21938
identifier_str_mv 0748-7304
10.1177/07487304251378607
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv Journal of Biological Rhythms, 2025
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 12.823554

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