Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasma

Autores
Carranza Martin, Ana Cristina; Nickles, Kirsten; Sherlock, Danielle Nicole; Relling, Alejandro E.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Carranza Martin, Ana Cristina. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; Argentina
Fil: Nickles, Kirsten. The Ohio State University. Department of Animal Science; Estados Unidos
Fil: Sherlock, Danielle Nicole. The Ohio State University. Department of Animal Science; Estados Unidos
Fil: Relling, Alejandro E. The Ohio State University. Department of Animal Science; Estados Unidos
Our aim was to evaluate the effects of increasing dietary levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) during the final third of gestation on plasma and milk fatty acid (FA) profiles of ewes and their offspring. Additionally, correlation between maternal and offspring plasma, colostrum, and milk FA profiles were examined. Seventy-two pregnant ewes (92.2 ± 2.94 kg body weight at d 100 of gestation) were blocked by body condition score and randomly assigned to one of three diets containing 0, 1, or 2% of a Ca salt of FA enriched with EPA and DHA during the last 50 d of gestation. Animals were housed in 8 pens per treatment (3 ewes per pen) during the supplementation period. At lambing, all ewes were penned together and offered a diet without FA supplementation. For FA profile, blood samples were collected from ewes at d -20 prepartum, at lambing, and d 15 postpartum. Lamb plasma samples were obtained at birth (0.5 to 8 h post-suckling). Colostrum (0.5 to 8 h postpartum) and milk (d 15 postpartum) were also collected. Data were analyzed using a randomized complete block design. The model included the fixed effects of the treatment for lamb plasma FA profile; and the fixed effects of treatment, time, and their interaction for ewe plasma and colostrum and milk FAprofile. Supplementation with EPA and DHA increased (diet by time interaction; P ≤ 0.06) the polyunsaturated FA (PUFA; C20:40, C20:5, and all omega 3) in ewe plasma until lambing but was similar between treatments 15 d post lambing. Colostrum from supplemented ewes showed greater PUFA (C22:5 and C22:6) concentrations and the difference decreased in the milk (diet by time interaction; P < 0.01), although the colostrum PUFA increase was not correlated with most individual plasma PUFA (C18:2, C20:3n6 and n3, C20:4, C20:5, C22:5, and C22:6), except for C18:3, which showed a positive association (P = 0.03). Furthermore, colostrum PUFA concentrations were positively correlated with the FA composition of lamb plasma post-suckling (P < 0.02). The PUFA concentrations were also increased in lamb plasma at lambing (P < 0.01), suggesting potential effects on neonatal lipid metabolism. These findings demonstrate that dietary supplementation with EPA and DHA, initiated 50 days before lambing and terminated at parturition, can alter the FA composition of maternal plasma, colostrum, and milk, and also influence the plasma FA profile of the offspring.
Fuente
Journal of Animal Science, 103, skaf366
Materia
RUMIANTE
NUTRICION ANIMAL
ALIMENTACION ANIMAL
PRODUCCION ANIMAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/21882
Acceder
id RIUCA_fbb0de9c8dd1e7503922ce334a8a7090
oai_identifier_str oai:ucacris:123456789/21882
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasmaCarranza Martin, Ana CristinaNickles, KirstenSherlock, Danielle NicoleRelling, Alejandro E.RUMIANTENUTRICION ANIMALALIMENTACION ANIMALPRODUCCION ANIMALFil: Carranza Martin, Ana Cristina. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; ArgentinaFil: Nickles, Kirsten. The Ohio State University. Department of Animal Science; Estados UnidosFil: Sherlock, Danielle Nicole. The Ohio State University. Department of Animal Science; Estados UnidosFil: Relling, Alejandro E. The Ohio State University. Department of Animal Science; Estados UnidosOur aim was to evaluate the effects of increasing dietary levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) during the final third of gestation on plasma and milk fatty acid (FA) profiles of ewes and their offspring. Additionally, correlation between maternal and offspring plasma, colostrum, and milk FA profiles were examined. Seventy-two pregnant ewes (92.2 ± 2.94 kg body weight at d 100 of gestation) were blocked by body condition score and randomly assigned to one of three diets containing 0, 1, or 2% of a Ca salt of FA enriched with EPA and DHA during the last 50 d of gestation. Animals were housed in 8 pens per treatment (3 ewes per pen) during the supplementation period. At lambing, all ewes were penned together and offered a diet without FA supplementation. For FA profile, blood samples were collected from ewes at d -20 prepartum, at lambing, and d 15 postpartum. Lamb plasma samples were obtained at birth (0.5 to 8 h post-suckling). Colostrum (0.5 to 8 h postpartum) and milk (d 15 postpartum) were also collected. Data were analyzed using a randomized complete block design. The model included the fixed effects of the treatment for lamb plasma FA profile; and the fixed effects of treatment, time, and their interaction for ewe plasma and colostrum and milk FAprofile. Supplementation with EPA and DHA increased (diet by time interaction; P ≤ 0.06) the polyunsaturated FA (PUFA; C20:40, C20:5, and all omega 3) in ewe plasma until lambing but was similar between treatments 15 d post lambing. Colostrum from supplemented ewes showed greater PUFA (C22:5 and C22:6) concentrations and the difference decreased in the milk (diet by time interaction; P < 0.01), although the colostrum PUFA increase was not correlated with most individual plasma PUFA (C18:2, C20:3n6 and n3, C20:4, C20:5, C22:5, and C22:6), except for C18:3, which showed a positive association (P = 0.03). Furthermore, colostrum PUFA concentrations were positively correlated with the FA composition of lamb plasma post-suckling (P < 0.02). The PUFA concentrations were also increased in lamb plasma at lambing (P < 0.01), suggesting potential effects on neonatal lipid metabolism. These findings demonstrate that dietary supplementation with EPA and DHA, initiated 50 days before lambing and terminated at parturition, can alter the FA composition of maternal plasma, colostrum, and milk, and also influence the plasma FA profile of the offspring.Oxford University Press2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/218820021-8812https://doi.org/10.1093/jas/skaf366Journal of Animal Science, 103, skaf366reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2026-10-01T12:03:35Zoai:ucacris:123456789/21882instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852026-10-01 12:03:35.694Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasma
title Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasma
spellingShingle Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasma
Carranza Martin, Ana Cristina
RUMIANTE
NUTRICION ANIMAL
ALIMENTACION ANIMAL
PRODUCCION ANIMAL
title_short Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasma
title_full Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasma
title_fullStr Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasma
title_full_unstemmed Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasma
title_sort Supplementation with eicosapentaenoic and docosahexaenoic acids during late gestation alters fatty acid profiles in ewe colostrum, milk, and plasma, and lamb plasma
dc.creator.none.fl_str_mv Carranza Martin, Ana Cristina
Nickles, Kirsten
Sherlock, Danielle Nicole
Relling, Alejandro E.
author Carranza Martin, Ana Cristina
author_facet Carranza Martin, Ana Cristina
Nickles, Kirsten
Sherlock, Danielle Nicole
Relling, Alejandro E.
author_role author
author2 Nickles, Kirsten
Sherlock, Danielle Nicole
Relling, Alejandro E.
author2_role author
author
author
dc.subject.none.fl_str_mv RUMIANTE
NUTRICION ANIMAL
ALIMENTACION ANIMAL
PRODUCCION ANIMAL
topic RUMIANTE
NUTRICION ANIMAL
ALIMENTACION ANIMAL
PRODUCCION ANIMAL
dc.description.none.fl_txt_mv Fil: Carranza Martin, Ana Cristina. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; Argentina
Fil: Nickles, Kirsten. The Ohio State University. Department of Animal Science; Estados Unidos
Fil: Sherlock, Danielle Nicole. The Ohio State University. Department of Animal Science; Estados Unidos
Fil: Relling, Alejandro E. The Ohio State University. Department of Animal Science; Estados Unidos
Our aim was to evaluate the effects of increasing dietary levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) during the final third of gestation on plasma and milk fatty acid (FA) profiles of ewes and their offspring. Additionally, correlation between maternal and offspring plasma, colostrum, and milk FA profiles were examined. Seventy-two pregnant ewes (92.2 ± 2.94 kg body weight at d 100 of gestation) were blocked by body condition score and randomly assigned to one of three diets containing 0, 1, or 2% of a Ca salt of FA enriched with EPA and DHA during the last 50 d of gestation. Animals were housed in 8 pens per treatment (3 ewes per pen) during the supplementation period. At lambing, all ewes were penned together and offered a diet without FA supplementation. For FA profile, blood samples were collected from ewes at d -20 prepartum, at lambing, and d 15 postpartum. Lamb plasma samples were obtained at birth (0.5 to 8 h post-suckling). Colostrum (0.5 to 8 h postpartum) and milk (d 15 postpartum) were also collected. Data were analyzed using a randomized complete block design. The model included the fixed effects of the treatment for lamb plasma FA profile; and the fixed effects of treatment, time, and their interaction for ewe plasma and colostrum and milk FAprofile. Supplementation with EPA and DHA increased (diet by time interaction; P ≤ 0.06) the polyunsaturated FA (PUFA; C20:40, C20:5, and all omega 3) in ewe plasma until lambing but was similar between treatments 15 d post lambing. Colostrum from supplemented ewes showed greater PUFA (C22:5 and C22:6) concentrations and the difference decreased in the milk (diet by time interaction; P < 0.01), although the colostrum PUFA increase was not correlated with most individual plasma PUFA (C18:2, C20:3n6 and n3, C20:4, C20:5, C22:5, and C22:6), except for C18:3, which showed a positive association (P = 0.03). Furthermore, colostrum PUFA concentrations were positively correlated with the FA composition of lamb plasma post-suckling (P < 0.02). The PUFA concentrations were also increased in lamb plasma at lambing (P < 0.01), suggesting potential effects on neonatal lipid metabolism. These findings demonstrate that dietary supplementation with EPA and DHA, initiated 50 days before lambing and terminated at parturition, can alter the FA composition of maternal plasma, colostrum, and milk, and also influence the plasma FA profile of the offspring.
description Fil: Carranza Martin, Ana Cristina. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; Argentina
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/21882
0021-8812
https://doi.org/10.1093/jas/skaf366
url https://repositorio.uca.edu.ar/handle/123456789/21882
https://doi.org/10.1093/jas/skaf366
identifier_str_mv 0021-8812
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Journal of Animal Science, 103, skaf366
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 12.853507

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