GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic...

Autores
Collavino, Mónica Mariana; Riccillo, Pablo Martín; Grasso, Daniel Hector; Crespi, Martín; Aguilar, Mario Orlando
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentina.
Fil: Collavino, Mónica Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.
Fil: Riccillo, Pablo Martín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.
Fil: Grasso, Daniel Hector. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.
Fil: Crespi, Martín. Centre Nacional de la Recherche Scientifique. Institut des Sciences Vegetales; Francia.
Fil: Aguilar, Mario Orlando. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.
The guaB mutant strain Rhizobium tropici CIAT8999-10T is defective in symbiosis with common bean, forming nodules that lack rhizobial content. In order to investigate the timing of the guaB requirement during the nodule formation on the host common bean by the strain CIAT899-10.T, we constructed gene fusions in which the guaB gene is expressed under the control of the symbiotic promoters nodA, bacA, and nifH. Our data indicated that the guaB is required from the early stages of nodulation because full recovery of the wild-type phenotype was accomplished by the nodA-guaB fusion. In addition, we have constructed a guaB mutant derived from Sinorhizobium meliloti 1021, and shown that, unlike R. tropici, the guaB S. meliloti mutant is auxotrophic for guanine and induces wild-type nodules on alfalfa and Medicago truncatula. The guaB R. tropici mutant also is defective in its symbiosis with Macroptilium atropurpureum and Vigna unguiculata but normal with Leucaena leucocephala. These results show that the requirement of the rhizobial guaB for symbiosis is found to be associated with host plants that form determinate type of nodules.
Fuente
Molecular plant-microbe interactions, 2005, vol. 18, no. 7, p. 742-750.
Materia
Nodule development
GuaB
Rhizobium tropici
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/60116
Acceder
id RIUNNE_4cb90dece633370a4cb7ae455c0d4196
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/60116
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interactionCollavino, Mónica MarianaRiccillo, Pablo MartínGrasso, Daniel HectorCrespi, MartínAguilar, Mario OrlandoNodule developmentGuaBRhizobium tropiciFil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentina.Fil: Collavino, Mónica Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.Fil: Riccillo, Pablo Martín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.Fil: Grasso, Daniel Hector. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.Fil: Crespi, Martín. Centre Nacional de la Recherche Scientifique. Institut des Sciences Vegetales; Francia.Fil: Aguilar, Mario Orlando. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.The guaB mutant strain Rhizobium tropici CIAT8999-10T is defective in symbiosis with common bean, forming nodules that lack rhizobial content. In order to investigate the timing of the guaB requirement during the nodule formation on the host common bean by the strain CIAT899-10.T, we constructed gene fusions in which the guaB gene is expressed under the control of the symbiotic promoters nodA, bacA, and nifH. Our data indicated that the guaB is required from the early stages of nodulation because full recovery of the wild-type phenotype was accomplished by the nodA-guaB fusion. In addition, we have constructed a guaB mutant derived from Sinorhizobium meliloti 1021, and shown that, unlike R. tropici, the guaB S. meliloti mutant is auxotrophic for guanine and induces wild-type nodules on alfalfa and Medicago truncatula. The guaB R. tropici mutant also is defective in its symbiosis with Macroptilium atropurpureum and Vigna unguiculata but normal with Leucaena leucocephala. These results show that the requirement of the rhizobial guaB for symbiosis is found to be associated with host plants that form determinate type of nodules.The American Phytopathological Society2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 742-750application/pdfCollavino, Mónica Mariana, et al., 2005. GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction. Molecular plant-microbe interactions. St. Paul: The American Phytopathological Society, vol. 18, no. 7, p. 742-750. E-ISSN 1943-7706. DOI https://doi.org/10.1094/MPMI-18-0742http://repositorio.unne.edu.ar/handle/123456789/60116Molecular plant-microbe interactions, 2005, vol. 18, no. 7, p. 742-750.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://doi.org/10.1094/MPMI-18-0742info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2026-09-24T12:46:39Zoai:repositorio.unne.edu.ar:123456789/60116instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712026-09-24 12:46:40.621Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction
title GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction
spellingShingle GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction
Collavino, Mónica Mariana
Nodule development
GuaB
Rhizobium tropici
title_short GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction
title_full GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction
title_fullStr GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction
title_full_unstemmed GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction
title_sort GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction
dc.creator.none.fl_str_mv Collavino, Mónica Mariana
Riccillo, Pablo Martín
Grasso, Daniel Hector
Crespi, Martín
Aguilar, Mario Orlando
author Collavino, Mónica Mariana
author_facet Collavino, Mónica Mariana
Riccillo, Pablo Martín
Grasso, Daniel Hector
Crespi, Martín
Aguilar, Mario Orlando
author_role author
author2 Riccillo, Pablo Martín
Grasso, Daniel Hector
Crespi, Martín
Aguilar, Mario Orlando
author2_role author
author
author
author
dc.subject.none.fl_str_mv Nodule development
GuaB
Rhizobium tropici
topic Nodule development
GuaB
Rhizobium tropici
dc.description.none.fl_txt_mv Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentina.
Fil: Collavino, Mónica Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.
Fil: Riccillo, Pablo Martín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.
Fil: Grasso, Daniel Hector. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.
Fil: Crespi, Martín. Centre Nacional de la Recherche Scientifique. Institut des Sciences Vegetales; Francia.
Fil: Aguilar, Mario Orlando. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Bioquímica y Biología Molecular; Argentina.
The guaB mutant strain Rhizobium tropici CIAT8999-10T is defective in symbiosis with common bean, forming nodules that lack rhizobial content. In order to investigate the timing of the guaB requirement during the nodule formation on the host common bean by the strain CIAT899-10.T, we constructed gene fusions in which the guaB gene is expressed under the control of the symbiotic promoters nodA, bacA, and nifH. Our data indicated that the guaB is required from the early stages of nodulation because full recovery of the wild-type phenotype was accomplished by the nodA-guaB fusion. In addition, we have constructed a guaB mutant derived from Sinorhizobium meliloti 1021, and shown that, unlike R. tropici, the guaB S. meliloti mutant is auxotrophic for guanine and induces wild-type nodules on alfalfa and Medicago truncatula. The guaB R. tropici mutant also is defective in its symbiosis with Macroptilium atropurpureum and Vigna unguiculata but normal with Leucaena leucocephala. These results show that the requirement of the rhizobial guaB for symbiosis is found to be associated with host plants that form determinate type of nodules.
description Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Collavino, Mónica Mariana, et al., 2005. GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction. Molecular plant-microbe interactions. St. Paul: The American Phytopathological Society, vol. 18, no. 7, p. 742-750. E-ISSN 1943-7706. DOI https://doi.org/10.1094/MPMI-18-0742
http://repositorio.unne.edu.ar/handle/123456789/60116
identifier_str_mv Collavino, Mónica Mariana, et al., 2005. GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules, but it is dispensable for the Sinorhizobium meliloti–alfalfa symbiotic interaction. Molecular plant-microbe interactions. St. Paul: The American Phytopathological Society, vol. 18, no. 7, p. 742-750. E-ISSN 1943-7706. DOI https://doi.org/10.1094/MPMI-18-0742
url http://repositorio.unne.edu.ar/handle/123456789/60116
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1094/MPMI-18-0742
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 742-750
application/pdf
dc.publisher.none.fl_str_mv The American Phytopathological Society
publisher.none.fl_str_mv The American Phytopathological Society
dc.source.none.fl_str_mv Molecular plant-microbe interactions, 2005, vol. 18, no. 7, p. 742-750.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 13.24418

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