Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum

Autores
Vega, Juan Manuel; Vega, María Sol; Quarin, C. C.; Leblanc, Olivier; Siena, Lorena Adelina; Ortiz, Juan Pablo Amelio
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fil: Vega, Juan Manuel. Universidad Nacional de Rosario; Argentina.
Fil: Vega, María Sol. Universidad Nacional de Rosario; Argentina.
Fil: Quarin, C. C. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Quarin, C. C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Leblanc, Olivier. Université de Montpellier; Francia.
Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario; Argentina.
Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario; Argentina.
P. notatum forms an agamic complex composed by sexual self-sterile diploid and aposporous apomictic self-fertile tetraploid plants. Apospory (a component of apomixis) is transmitted by male gametes as a single-dominant factor with distorted segregation ratios (6-3 sex:1 apo). Most natural apomicts are obligate, however highly-sexual apomictic genotypes were experimentally obtained and used as female parents in crosses with apomictic donors. Here, we analyzed the transmission of apospory by female reduced gametes. To achieve this, three populations were developed: Pop_S (n= 6 ) derived from self-pollination of Q3664, a highly-sexual facultative apomictic plant with white (recesive) stigmas; Pop_C (n= 12) from the cross between Q3664 and C4-4x, a fully sexual plant with purpura (dominant) stigmas; and, Pop_A (n= 20) from open pollination of Q4117, a natural apomictic accession. Progeny origin (hybrid/maternal clone) was determined by observation of stigmas color and molecular analysis. The reproductive mode was assessed by observation of ovules at anthesis (meiotic vs. aposporous embryo sacs). Phenotypic and molecular analyses showed that all Pop_S and Pop_C plants but one of Pop_S derived from sexuality, while all plants of Pop_A resulted from apomixis. Pop_S showed 5 aposporous and 1 sexual plants (?2 1 3:1 apo:sex= 0.22) and Pop_C comprised 3 aposporous and 9 sexual individuals (?2 1 1:1= 3.0). Apospory expressivity in hybrids range 3-13%. Our results indicate that apomixis is transmitted by reduced female gametes, although maternal inherited apospory showed low expressivity.
Materia
Tetraploid plants
Molecular analysis
Apospory transmission
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/61145
Acceder
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network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatumVega, Juan ManuelVega, María SolQuarin, C. C.Leblanc, OlivierSiena, Lorena AdelinaOrtiz, Juan Pablo AmelioTetraploid plantsMolecular analysisApospory transmissionFil: Vega, Juan Manuel. Universidad Nacional de Rosario; Argentina.Fil: Vega, María Sol. Universidad Nacional de Rosario; Argentina.Fil: Quarin, C. C. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Quarin, C. C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Leblanc, Olivier. Université de Montpellier; Francia.Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario; Argentina.Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario; Argentina.P. notatum forms an agamic complex composed by sexual self-sterile diploid and aposporous apomictic self-fertile tetraploid plants. Apospory (a component of apomixis) is transmitted by male gametes as a single-dominant factor with distorted segregation ratios (6-3 sex:1 apo). Most natural apomicts are obligate, however highly-sexual apomictic genotypes were experimentally obtained and used as female parents in crosses with apomictic donors. Here, we analyzed the transmission of apospory by female reduced gametes. To achieve this, three populations were developed: Pop_S (n= 6 ) derived from self-pollination of Q3664, a highly-sexual facultative apomictic plant with white (recesive) stigmas; Pop_C (n= 12) from the cross between Q3664 and C4-4x, a fully sexual plant with purpura (dominant) stigmas; and, Pop_A (n= 20) from open pollination of Q4117, a natural apomictic accession. Progeny origin (hybrid/maternal clone) was determined by observation of stigmas color and molecular analysis. The reproductive mode was assessed by observation of ovules at anthesis (meiotic vs. aposporous embryo sacs). Phenotypic and molecular analyses showed that all Pop_S and Pop_C plants but one of Pop_S derived from sexuality, while all plants of Pop_A resulted from apomixis. Pop_S showed 5 aposporous and 1 sexual plants (?2 1 3:1 apo:sex= 0.22) and Pop_C comprised 3 aposporous and 9 sexual individuals (?2 1 1:1= 3.0). Apospory expressivity in hybrids range 3-13%. Our results indicate that apomixis is transmitted by reduced female gametes, although maternal inherited apospory showed low expressivity.Asociación Latinoamericano de GenéticaSociedad Argentina de Genética2019-10info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfp. 244-244application/pdfVega, Juan Manuel, et al., 2019. Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum. En: XVII Congreso Latinoamericano de Genética; XLVII Congreso Argentino de Genética. Mendoza: Asociación Latinoamericano de Genética; Sociedad Argentina de Genética, p. 244-244.1852-6233https://repositorio.unne.edu.ar/handle/123456789/61145enghttps://sag.org.ar/jbag/wp-content/uploads/2023/10/VXXXIII_2_Suppl_25102023.pdfinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentinareponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordeste2026-09-24T12:48:11Zoai:repositorio.unne.edu.ar:123456789/61145instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712026-09-24 12:48:12.808Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum
title Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum
spellingShingle Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum
Vega, Juan Manuel
Tetraploid plants
Molecular analysis
Apospory transmission
title_short Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum
title_full Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum
title_fullStr Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum
title_full_unstemmed Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum
title_sort Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum
dc.creator.none.fl_str_mv Vega, Juan Manuel
Vega, María Sol
Quarin, C. C.
Leblanc, Olivier
Siena, Lorena Adelina
Ortiz, Juan Pablo Amelio
author Vega, Juan Manuel
author_facet Vega, Juan Manuel
Vega, María Sol
Quarin, C. C.
Leblanc, Olivier
Siena, Lorena Adelina
Ortiz, Juan Pablo Amelio
author_role author
author2 Vega, María Sol
Quarin, C. C.
Leblanc, Olivier
Siena, Lorena Adelina
Ortiz, Juan Pablo Amelio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Tetraploid plants
Molecular analysis
Apospory transmission
topic Tetraploid plants
Molecular analysis
Apospory transmission
dc.description.none.fl_txt_mv Fil: Vega, Juan Manuel. Universidad Nacional de Rosario; Argentina.
Fil: Vega, María Sol. Universidad Nacional de Rosario; Argentina.
Fil: Quarin, C. C. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Quarin, C. C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Leblanc, Olivier. Université de Montpellier; Francia.
Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario; Argentina.
Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario; Argentina.
P. notatum forms an agamic complex composed by sexual self-sterile diploid and aposporous apomictic self-fertile tetraploid plants. Apospory (a component of apomixis) is transmitted by male gametes as a single-dominant factor with distorted segregation ratios (6-3 sex:1 apo). Most natural apomicts are obligate, however highly-sexual apomictic genotypes were experimentally obtained and used as female parents in crosses with apomictic donors. Here, we analyzed the transmission of apospory by female reduced gametes. To achieve this, three populations were developed: Pop_S (n= 6 ) derived from self-pollination of Q3664, a highly-sexual facultative apomictic plant with white (recesive) stigmas; Pop_C (n= 12) from the cross between Q3664 and C4-4x, a fully sexual plant with purpura (dominant) stigmas; and, Pop_A (n= 20) from open pollination of Q4117, a natural apomictic accession. Progeny origin (hybrid/maternal clone) was determined by observation of stigmas color and molecular analysis. The reproductive mode was assessed by observation of ovules at anthesis (meiotic vs. aposporous embryo sacs). Phenotypic and molecular analyses showed that all Pop_S and Pop_C plants but one of Pop_S derived from sexuality, while all plants of Pop_A resulted from apomixis. Pop_S showed 5 aposporous and 1 sexual plants (?2 1 3:1 apo:sex= 0.22) and Pop_C comprised 3 aposporous and 9 sexual individuals (?2 1 1:1= 3.0). Apospory expressivity in hybrids range 3-13%. Our results indicate that apomixis is transmitted by reduced female gametes, although maternal inherited apospory showed low expressivity.
description Fil: Vega, Juan Manuel. Universidad Nacional de Rosario; Argentina.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv Vega, Juan Manuel, et al., 2019. Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum. En: XVII Congreso Latinoamericano de Genética; XLVII Congreso Argentino de Genética. Mendoza: Asociación Latinoamericano de Genética; Sociedad Argentina de Genética, p. 244-244.
1852-6233
https://repositorio.unne.edu.ar/handle/123456789/61145
identifier_str_mv Vega, Juan Manuel, et al., 2019. Analysis of apospory transmission through reduced female gametes in tetraploid Paspalum notatum. En: XVII Congreso Latinoamericano de Genética; XLVII Congreso Argentino de Genética. Mendoza: Asociación Latinoamericano de Genética; Sociedad Argentina de Genética, p. 244-244.
1852-6233
url https://repositorio.unne.edu.ar/handle/123456789/61145
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://sag.org.ar/jbag/wp-content/uploads/2023/10/VXXXIII_2_Suppl_25102023.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 244-244
application/pdf
dc.publisher.none.fl_str_mv Asociación Latinoamericano de Genética
Sociedad Argentina de Genética
publisher.none.fl_str_mv Asociación Latinoamericano de Genética
Sociedad Argentina de Genética
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 13.265058

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