Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)

Autores
Seijo, José Guillermo; Samoluk, Sergio Sebastián; Chalup, Laura María Isabel; Robledo Dobladez, German Ariel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Samoluk, Sergio Sebastián. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Samoluk, Sergio Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Chalup, Laura María Isabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Chalup, Laura María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Robledo Dobladez, German Ariel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Robledo Dobladez, German Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Section Arachis is composed of diploid species (2n=20, 18) with A, B, D, F, G or K genomes and two AABB tetraploids, the peanut and its direct antecessor A. monticola. In this study, the role of the repetitive fraction in the differentiation of the genomes at diploid and polyploid levels was investigated. For that purpose, different repetitive sequences were isolated (PCR), characterized (sequence analysis), quantified (dot blot) and chromosome mapped (FISH) in representative species of each genome. Additionally, the global pattern of 5-methylcytosine was analyzed by immunocytochemistry. The retroelements Ty3 Gypsy and LINES, the CACTA-like transposons, and the TR2 satellite sequence were differentially represented and distributed on the chromosomes of the species belonging to different genomes. The distribution pattern of the 5-methylcytosine was distinctive between the A and B genomes. All the markers analyzed in the allotetraploids evidenced the sum of the patterns observed in their diploid progenitors. However, the cytosines of the B-genome of the tetraploids appeared hypermethylated with respect to its wild progenitor and nucleolar dominance of the A genome was observed in the polyploid taxa. These results evidenced that changes in the repetitive fraction and in the epigenetic patterns had played a key role in the genome differentiation at diploid level. However, the allopolyploidization did not affect the gross chromosome structure in the AABB taxa, but triggered a massive hypermethylation of the B genome.
Materia
Arachis species
Genome differentiation
Allopolyploidization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/61217
Acceder
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network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)Seijo, José GuillermoSamoluk, Sergio SebastiánChalup, Laura María IsabelRobledo Dobladez, German ArielArachis speciesGenome differentiationAllopolyploidizationFil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Samoluk, Sergio Sebastián. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Samoluk, Sergio Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Chalup, Laura María Isabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Chalup, Laura María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Robledo Dobladez, German Ariel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Robledo Dobladez, German Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Section Arachis is composed of diploid species (2n=20, 18) with A, B, D, F, G or K genomes and two AABB tetraploids, the peanut and its direct antecessor A. monticola. In this study, the role of the repetitive fraction in the differentiation of the genomes at diploid and polyploid levels was investigated. For that purpose, different repetitive sequences were isolated (PCR), characterized (sequence analysis), quantified (dot blot) and chromosome mapped (FISH) in representative species of each genome. Additionally, the global pattern of 5-methylcytosine was analyzed by immunocytochemistry. The retroelements Ty3 Gypsy and LINES, the CACTA-like transposons, and the TR2 satellite sequence were differentially represented and distributed on the chromosomes of the species belonging to different genomes. The distribution pattern of the 5-methylcytosine was distinctive between the A and B genomes. All the markers analyzed in the allotetraploids evidenced the sum of the patterns observed in their diploid progenitors. However, the cytosines of the B-genome of the tetraploids appeared hypermethylated with respect to its wild progenitor and nucleolar dominance of the A genome was observed in the polyploid taxa. These results evidenced that changes in the repetitive fraction and in the epigenetic patterns had played a key role in the genome differentiation at diploid level. However, the allopolyploidization did not affect the gross chromosome structure in the AABB taxa, but triggered a massive hypermethylation of the B genome.Asociación Latinoamericano de GenéticaSociedad Argentina de Genética2016-10-09info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfp. 26-26application/pdfSeijo, José Guillermo, et al., 2016. Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis). En: XVI Congreso Latinoamericano de Genética; IV Congreso de la Sociedad Uruguaya de Genética; XLIX Reunión Anual de la Sociedad de Genética de Chile; XLV Congreso Argentino de Genética. Montevideo: Asociación Latinoamericano de Genética; Sociedad Argentina de Genética, p. 26-26.1852-6233https://repositorio.unne.edu.ar/handle/123456789/61217enghttps://sag.org.ar/jbag/wp-content/uploads/2023/10/VXXXIII_2_Suppl_25102023.pdfinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentinareponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordeste2026-09-24T12:46:59Zoai:repositorio.unne.edu.ar:123456789/61217instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712026-09-24 12:46:59.632Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)
title Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)
spellingShingle Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)
Seijo, José Guillermo
Arachis species
Genome differentiation
Allopolyploidization
title_short Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)
title_full Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)
title_fullStr Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)
title_full_unstemmed Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)
title_sort Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis)
dc.creator.none.fl_str_mv Seijo, José Guillermo
Samoluk, Sergio Sebastián
Chalup, Laura María Isabel
Robledo Dobladez, German Ariel
author Seijo, José Guillermo
author_facet Seijo, José Guillermo
Samoluk, Sergio Sebastián
Chalup, Laura María Isabel
Robledo Dobladez, German Ariel
author_role author
author2 Samoluk, Sergio Sebastián
Chalup, Laura María Isabel
Robledo Dobladez, German Ariel
author2_role author
author
author
dc.subject.none.fl_str_mv Arachis species
Genome differentiation
Allopolyploidization
topic Arachis species
Genome differentiation
Allopolyploidization
dc.description.none.fl_txt_mv Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Samoluk, Sergio Sebastián. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Samoluk, Sergio Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Chalup, Laura María Isabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Chalup, Laura María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Robledo Dobladez, German Ariel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Robledo Dobladez, German Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Section Arachis is composed of diploid species (2n=20, 18) with A, B, D, F, G or K genomes and two AABB tetraploids, the peanut and its direct antecessor A. monticola. In this study, the role of the repetitive fraction in the differentiation of the genomes at diploid and polyploid levels was investigated. For that purpose, different repetitive sequences were isolated (PCR), characterized (sequence analysis), quantified (dot blot) and chromosome mapped (FISH) in representative species of each genome. Additionally, the global pattern of 5-methylcytosine was analyzed by immunocytochemistry. The retroelements Ty3 Gypsy and LINES, the CACTA-like transposons, and the TR2 satellite sequence were differentially represented and distributed on the chromosomes of the species belonging to different genomes. The distribution pattern of the 5-methylcytosine was distinctive between the A and B genomes. All the markers analyzed in the allotetraploids evidenced the sum of the patterns observed in their diploid progenitors. However, the cytosines of the B-genome of the tetraploids appeared hypermethylated with respect to its wild progenitor and nucleolar dominance of the A genome was observed in the polyploid taxa. These results evidenced that changes in the repetitive fraction and in the epigenetic patterns had played a key role in the genome differentiation at diploid level. However, the allopolyploidization did not affect the gross chromosome structure in the AABB taxa, but triggered a massive hypermethylation of the B genome.
description Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-09
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv Seijo, José Guillermo, et al., 2016. Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis). En: XVI Congreso Latinoamericano de Genética; IV Congreso de la Sociedad Uruguaya de Genética; XLIX Reunión Anual de la Sociedad de Genética de Chile; XLV Congreso Argentino de Genética. Montevideo: Asociación Latinoamericano de Genética; Sociedad Argentina de Genética, p. 26-26.
1852-6233
https://repositorio.unne.edu.ar/handle/123456789/61217
identifier_str_mv Seijo, José Guillermo, et al., 2016. Karyotype evidences of genome differentiation at diploid and polyploid levels in Arachis species (sect. Arachis). En: XVI Congreso Latinoamericano de Genética; IV Congreso de la Sociedad Uruguaya de Genética; XLIX Reunión Anual de la Sociedad de Genética de Chile; XLV Congreso Argentino de Genética. Montevideo: Asociación Latinoamericano de Genética; Sociedad Argentina de Genética, p. 26-26.
1852-6233
url https://repositorio.unne.edu.ar/handle/123456789/61217
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://sag.org.ar/jbag/wp-content/uploads/2023/10/VXXXIII_2_Suppl_25102023.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 26-26
application/pdf
dc.publisher.none.fl_str_mv Asociación Latinoamericano de Genética
Sociedad Argentina de Genética
publisher.none.fl_str_mv Asociación Latinoamericano de Genética
Sociedad Argentina de Genética
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 13.265058

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