Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum

Autores
Sartor, María Esperanza; Espinoza, Francisco; Seijo, José Guillermo; González, Ana María; Pessino, Silvina Claudia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Espinoza, Francisco. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Pessino, Silvina Claudia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
In former expression analysis aimed at identifying genes differentially expressed in reproductive tissues of apomictic and sexual genotypes, we isolated a differentially expressed tag (experimental code N19), which kept similarity with checkpoint homologue CHK1. This candidate gene showed a high expression level, and was found upregulated in sexual plants. The objective of this work was to better characterize N19 structure and expression in reproductive tissues of apomictic and sexual plants of P. notatum. Relative expression quantitation using q-RTPCR revealed heterochronic temporal expression patterns, with minor upregulation in apomictic plant at premeiosis and meiosis, downregulation at postmeiosis and equal expression at anthesis. Spatial expression patterns were examined by using in situ RNA hybridization on reproductive tissues at meiosis and anthesis. During meiosis, the antisense probe revealed a strong signal in the teguments and nucella of the apomictic plant, but only in the area of the megaspore mother cell of the sexual plant. In anthesis, both reproductive types (apomictic and sexual) displayed a uniform expression pattern when hybridized with the antisense probe, displaying signal in teguments, egg apparatus and polar nuclei. No signal was observed with the sense probe. Rapid amplification of cDNA ends (RACE) experiments allowed the isolation of single bands, which will be sequenced in order to characterize the transcript full-length structure. Our results showed that the expression pattern of N19 actually differs between both reproductive types.
Materia
Paspalum notatum
Candidate gene
Experimental code N19
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/60489
Acceder
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oai_identifier_str oai:repositorio.unne.edu.ar:123456789/60489
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatumSartor, María EsperanzaEspinoza, FranciscoSeijo, José GuillermoGonzález, Ana MaríaPessino, Silvina ClaudiaPaspalum notatumCandidate geneExperimental code N19Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Espinoza, Francisco. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Pessino, Silvina Claudia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.In former expression analysis aimed at identifying genes differentially expressed in reproductive tissues of apomictic and sexual genotypes, we isolated a differentially expressed tag (experimental code N19), which kept similarity with checkpoint homologue CHK1. This candidate gene showed a high expression level, and was found upregulated in sexual plants. The objective of this work was to better characterize N19 structure and expression in reproductive tissues of apomictic and sexual plants of P. notatum. Relative expression quantitation using q-RTPCR revealed heterochronic temporal expression patterns, with minor upregulation in apomictic plant at premeiosis and meiosis, downregulation at postmeiosis and equal expression at anthesis. Spatial expression patterns were examined by using in situ RNA hybridization on reproductive tissues at meiosis and anthesis. During meiosis, the antisense probe revealed a strong signal in the teguments and nucella of the apomictic plant, but only in the area of the megaspore mother cell of the sexual plant. In anthesis, both reproductive types (apomictic and sexual) displayed a uniform expression pattern when hybridized with the antisense probe, displaying signal in teguments, egg apparatus and polar nuclei. No signal was observed with the sense probe. Rapid amplification of cDNA ends (RACE) experiments allowed the isolation of single bands, which will be sequenced in order to characterize the transcript full-length structure. Our results showed that the expression pattern of N19 actually differs between both reproductive types.Asociación Latinoamericano de GenéticaSociedad Argentina de Genética2012-10-28info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfp. 164-165application/pdfSartor, María Esperanza, et al., 2012. Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum. En: XV Congreso Latinoamericano de Genética; XLI Congreso Argentino de Genética; XLV Congreso de la Sociedad de Genética de Chile; II Reunión Regional SAG-Litoral. Rosario: Asociación Latinoamericano de Genética; Sociedad Argentina de Genética, p. 164-165.1852-6233https://repositorio.unne.edu.ar/handle/123456789/60489enghttps://sag.org.ar/jbag/wp-content/uploads/2023/10/VXXXIII_Suppl1_2022_25102023.pdfinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentinareponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordeste2026-09-24T12:49:04Zoai:repositorio.unne.edu.ar:123456789/60489instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712026-09-24 12:49:04.594Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum
title Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum
spellingShingle Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum
Sartor, María Esperanza
Paspalum notatum
Candidate gene
Experimental code N19
title_short Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum
title_full Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum
title_fullStr Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum
title_full_unstemmed Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum
title_sort Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum
dc.creator.none.fl_str_mv Sartor, María Esperanza
Espinoza, Francisco
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
author Sartor, María Esperanza
author_facet Sartor, María Esperanza
Espinoza, Francisco
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
author_role author
author2 Espinoza, Francisco
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Paspalum notatum
Candidate gene
Experimental code N19
topic Paspalum notatum
Candidate gene
Experimental code N19
dc.description.none.fl_txt_mv Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Espinoza, Francisco. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Pessino, Silvina Claudia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
In former expression analysis aimed at identifying genes differentially expressed in reproductive tissues of apomictic and sexual genotypes, we isolated a differentially expressed tag (experimental code N19), which kept similarity with checkpoint homologue CHK1. This candidate gene showed a high expression level, and was found upregulated in sexual plants. The objective of this work was to better characterize N19 structure and expression in reproductive tissues of apomictic and sexual plants of P. notatum. Relative expression quantitation using q-RTPCR revealed heterochronic temporal expression patterns, with minor upregulation in apomictic plant at premeiosis and meiosis, downregulation at postmeiosis and equal expression at anthesis. Spatial expression patterns were examined by using in situ RNA hybridization on reproductive tissues at meiosis and anthesis. During meiosis, the antisense probe revealed a strong signal in the teguments and nucella of the apomictic plant, but only in the area of the megaspore mother cell of the sexual plant. In anthesis, both reproductive types (apomictic and sexual) displayed a uniform expression pattern when hybridized with the antisense probe, displaying signal in teguments, egg apparatus and polar nuclei. No signal was observed with the sense probe. Rapid amplification of cDNA ends (RACE) experiments allowed the isolation of single bands, which will be sequenced in order to characterize the transcript full-length structure. Our results showed that the expression pattern of N19 actually differs between both reproductive types.
description Fil: Sartor, María Esperanza. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-28
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv Sartor, María Esperanza, et al., 2012. Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum. En: XV Congreso Latinoamericano de Genética; XLI Congreso Argentino de Genética; XLV Congreso de la Sociedad de Genética de Chile; II Reunión Regional SAG-Litoral. Rosario: Asociación Latinoamericano de Genética; Sociedad Argentina de Genética, p. 164-165.
1852-6233
https://repositorio.unne.edu.ar/handle/123456789/60489
identifier_str_mv Sartor, María Esperanza, et al., 2012. Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum. En: XV Congreso Latinoamericano de Genética; XLI Congreso Argentino de Genética; XLV Congreso de la Sociedad de Genética de Chile; II Reunión Regional SAG-Litoral. Rosario: Asociación Latinoamericano de Genética; Sociedad Argentina de Genética, p. 164-165.
1852-6233
url https://repositorio.unne.edu.ar/handle/123456789/60489
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://sag.org.ar/jbag/wp-content/uploads/2023/10/VXXXIII_Suppl1_2022_25102023.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 164-165
application/pdf
dc.publisher.none.fl_str_mv Asociación Latinoamericano de Genética
Sociedad Argentina de Genética
publisher.none.fl_str_mv Asociación Latinoamericano de Genética
Sociedad Argentina de Genética
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 13.265058

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