Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>

Autores
Davyt Colo, Belén; Girotti, Juan Roberto; González, Andrés; Pedrini, Nicolás
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Entomopathogenic fungi such as Beauveria bassiana are extensively used for the control of insect pests worldwide. They infect mostly by adhesion to the insect surface and penetration through the cuticle. However, some insects, such as the red flour beetle Tribolium castaneum (Herbst), have evolved resistance by embedding their cuticle with antifungal compounds. Thus, they avoid fungal germination on the cuticle, which result in low susceptibility to entomopathogenic fungi. In adult T. castaneum, these antifungals are the well-known defensive compounds methyl-1,4- and ethyl-1,4- benzoquinone. In this study, we added B. bassiana conidia on the diet of adult beetles to study the effect of the entomopathogen on the secretion and detection of the beetle volatile blend containing both benzoquinones. The compounds were analyzed by solid phase microextraction coupled to gas chromatography–flame ionization detection, and were detected by electroantennography. In addition, we measured the expression level of four genes encoding for two odorant-binding proteins (OBP), one chemosensory protein (CSP), and one odorant receptor (OR) in both healthy and fungustreated insects. Significant alterations in the secretion of both benzoquinones, as well as in the perception of methyl-1,4-benzoquinone, were found in fungus-treated insects. TcOBP7D, TcOBP0A and TcCSP3A genes were down-regulated in insects fed conidia for 12 and 48 h, and the latter gene was up-regulated in 72 h samples. TcOR1 expression was not altered at the feeding times studied. We conclude that fungus-treated insects alter both secretion and perception of benzoquinones, but additional functional and genetic studies are needed to fully understand the effects of fungal infection on the insect chemical ecology.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Biología
chemical ecology
benzoquinones
gene expression
odorant-binding proteins
chemosensory proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/149686
Acceder
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spelling Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>Davyt Colo, BelénGirotti, Juan RobertoGonzález, AndrésPedrini, NicolásBiologíachemical ecologybenzoquinonesgene expressionodorant-binding proteinschemosensory proteinsEntomopathogenic fungi such as Beauveria bassiana are extensively used for the control of insect pests worldwide. They infect mostly by adhesion to the insect surface and penetration through the cuticle. However, some insects, such as the red flour beetle Tribolium castaneum (Herbst), have evolved resistance by embedding their cuticle with antifungal compounds. Thus, they avoid fungal germination on the cuticle, which result in low susceptibility to entomopathogenic fungi. In adult T. castaneum, these antifungals are the well-known defensive compounds methyl-1,4- and ethyl-1,4- benzoquinone. In this study, we added B. bassiana conidia on the diet of adult beetles to study the effect of the entomopathogen on the secretion and detection of the beetle volatile blend containing both benzoquinones. The compounds were analyzed by solid phase microextraction coupled to gas chromatography–flame ionization detection, and were detected by electroantennography. In addition, we measured the expression level of four genes encoding for two odorant-binding proteins (OBP), one chemosensory protein (CSP), and one odorant receptor (OR) in both healthy and fungustreated insects. Significant alterations in the secretion of both benzoquinones, as well as in the perception of methyl-1,4-benzoquinone, were found in fungus-treated insects. TcOBP7D, TcOBP0A and TcCSP3A genes were down-regulated in insects fed conidia for 12 and 48 h, and the latter gene was up-regulated in 72 h samples. TcOR1 expression was not altered at the feeding times studied. We conclude that fungus-treated insects alter both secretion and perception of benzoquinones, but additional functional and genetic studies are needed to fully understand the effects of fungal infection on the insect chemical ecology.Instituto de Investigaciones Bioquímicas de La Plata2022-04-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/149686enginfo:eu-repo/semantics/altIdentifier/issn/2076-0817info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens11050487info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2026-06-23T11:01:05Zoai:sedici.unlp.edu.ar:10915/149686Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292026-06-23 11:01:05.508SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
spellingShingle Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
Davyt Colo, Belén
Biología
chemical ecology
benzoquinones
gene expression
odorant-binding proteins
chemosensory proteins
title_short Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title_full Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title_fullStr Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title_full_unstemmed Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title_sort Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
dc.creator.none.fl_str_mv Davyt Colo, Belén
Girotti, Juan Roberto
González, Andrés
Pedrini, Nicolás
author Davyt Colo, Belén
author_facet Davyt Colo, Belén
Girotti, Juan Roberto
González, Andrés
Pedrini, Nicolás
author_role author
author2 Girotti, Juan Roberto
González, Andrés
Pedrini, Nicolás
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
chemical ecology
benzoquinones
gene expression
odorant-binding proteins
chemosensory proteins
topic Biología
chemical ecology
benzoquinones
gene expression
odorant-binding proteins
chemosensory proteins
dc.description.none.fl_txt_mv Entomopathogenic fungi such as Beauveria bassiana are extensively used for the control of insect pests worldwide. They infect mostly by adhesion to the insect surface and penetration through the cuticle. However, some insects, such as the red flour beetle Tribolium castaneum (Herbst), have evolved resistance by embedding their cuticle with antifungal compounds. Thus, they avoid fungal germination on the cuticle, which result in low susceptibility to entomopathogenic fungi. In adult T. castaneum, these antifungals are the well-known defensive compounds methyl-1,4- and ethyl-1,4- benzoquinone. In this study, we added B. bassiana conidia on the diet of adult beetles to study the effect of the entomopathogen on the secretion and detection of the beetle volatile blend containing both benzoquinones. The compounds were analyzed by solid phase microextraction coupled to gas chromatography–flame ionization detection, and were detected by electroantennography. In addition, we measured the expression level of four genes encoding for two odorant-binding proteins (OBP), one chemosensory protein (CSP), and one odorant receptor (OR) in both healthy and fungustreated insects. Significant alterations in the secretion of both benzoquinones, as well as in the perception of methyl-1,4-benzoquinone, were found in fungus-treated insects. TcOBP7D, TcOBP0A and TcCSP3A genes were down-regulated in insects fed conidia for 12 and 48 h, and the latter gene was up-regulated in 72 h samples. TcOR1 expression was not altered at the feeding times studied. We conclude that fungus-treated insects alter both secretion and perception of benzoquinones, but additional functional and genetic studies are needed to fully understand the effects of fungal infection on the insect chemical ecology.
Instituto de Investigaciones Bioquímicas de La Plata
description Entomopathogenic fungi such as Beauveria bassiana are extensively used for the control of insect pests worldwide. They infect mostly by adhesion to the insect surface and penetration through the cuticle. However, some insects, such as the red flour beetle Tribolium castaneum (Herbst), have evolved resistance by embedding their cuticle with antifungal compounds. Thus, they avoid fungal germination on the cuticle, which result in low susceptibility to entomopathogenic fungi. In adult T. castaneum, these antifungals are the well-known defensive compounds methyl-1,4- and ethyl-1,4- benzoquinone. In this study, we added B. bassiana conidia on the diet of adult beetles to study the effect of the entomopathogen on the secretion and detection of the beetle volatile blend containing both benzoquinones. The compounds were analyzed by solid phase microextraction coupled to gas chromatography–flame ionization detection, and were detected by electroantennography. In addition, we measured the expression level of four genes encoding for two odorant-binding proteins (OBP), one chemosensory protein (CSP), and one odorant receptor (OR) in both healthy and fungustreated insects. Significant alterations in the secretion of both benzoquinones, as well as in the perception of methyl-1,4-benzoquinone, were found in fungus-treated insects. TcOBP7D, TcOBP0A and TcCSP3A genes were down-regulated in insects fed conidia for 12 and 48 h, and the latter gene was up-regulated in 72 h samples. TcOR1 expression was not altered at the feeding times studied. We conclude that fungus-treated insects alter both secretion and perception of benzoquinones, but additional functional and genetic studies are needed to fully understand the effects of fungal infection on the insect chemical ecology.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/149686
url http://sedici.unlp.edu.ar/handle/10915/149686
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2076-0817
info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens11050487
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
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instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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