Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput Development

Autores
Vio, Santiago Adolfo; Paiva González, Karen Belén; Gortari, María Cecilia; Galar, María Lina; Pistorio, Mariano; Luna, María Flavia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant growth-promoting bacteria (PGPB) represent a sustainable alternative to synthetic inputs in horticultural systems; however, their bioprospecting is hindered by the absence of integrative, performance-oriented selection strategies. In this study, a comprehensive collection of 259 bacterial isolates associated with tomato plants was systematically screened to identify strains with biocontrol and plant growth-promoting potential. Isolates were characterized in vitro for potential plant colonization ability, antifungal activity, and multiple plant growth-promoting mechanisms. These traits were integrated into composite indices and analyzed using multivariate approaches to guide the selection of promising isolates. Selected candidates were subsequently evaluated in vivo for biocontrol and plant growth at both seedling and productive stages, and most isolates exhibited consistent effects. Isolates from the genera Stenotrophomonas, Pseudomonas, and Bacillus reduced fungal disease incidence to 2–9% (control disease: 80%). A Bacillus isolate increased seedling biomass by 54% in lettuce and 38% in tomato. Under productive conditions, lettuce marketable weight increased by 21–37%, whereas tomato yield showed positive but non-significant increases (~21–25%) after inoculation with Pseudomonas or Bacillus isolates. Overall, this work provides a structured framework for PGPB bioprospecting and validation, combining laboratory screening, composite indices, multivariate analyses, and multi-stage in vivo assays under realistic horticultural conditions.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnología y Biología Molecular
Materia
Ciencias Agrarias
Bioprospecting
Plant growth-promoting bacteria
Biocontrol
Endophytic bacteria
Sustainable horticulture
Lettuce
Tomato
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/195661
Acceder
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network_name_str SEDICI (UNLP)
spelling Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput DevelopmentVio, Santiago AdolfoPaiva González, Karen BelénGortari, María CeciliaGalar, María LinaPistorio, MarianoLuna, María FlaviaCiencias AgrariasBioprospectingPlant growth-promoting bacteriaBiocontrolEndophytic bacteriaSustainable horticultureLettuceTomatoPlant growth-promoting bacteria (PGPB) represent a sustainable alternative to synthetic inputs in horticultural systems; however, their bioprospecting is hindered by the absence of integrative, performance-oriented selection strategies. In this study, a comprehensive collection of 259 bacterial isolates associated with tomato plants was systematically screened to identify strains with biocontrol and plant growth-promoting potential. Isolates were characterized in vitro for potential plant colonization ability, antifungal activity, and multiple plant growth-promoting mechanisms. These traits were integrated into composite indices and analyzed using multivariate approaches to guide the selection of promising isolates. Selected candidates were subsequently evaluated in vivo for biocontrol and plant growth at both seedling and productive stages, and most isolates exhibited consistent effects. Isolates from the genera Stenotrophomonas, Pseudomonas, and Bacillus reduced fungal disease incidence to 2–9% (control disease: 80%). A Bacillus isolate increased seedling biomass by 54% in lettuce and 38% in tomato. Under productive conditions, lettuce marketable weight increased by 21–37%, whereas tomato yield showed positive but non-significant increases (~21–25%) after inoculation with Pseudomonas or Bacillus isolates. Overall, this work provides a structured framework for PGPB bioprospecting and validation, combining laboratory screening, composite indices, multivariate analyses, and multi-stage in vivo assays under realistic horticultural conditions.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesInstituto de Biotecnología y Biología Molecular2026-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/195661enginfo:eu-repo/semantics/altIdentifier/issn/2077-0472info:eu-repo/semantics/altIdentifier/doi/10.3390/agriculture16050610info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2026-06-23T11:17:07Zoai:sedici.unlp.edu.ar:10915/195661Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292026-06-23 11:17:07.702SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput Development
title Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput Development
spellingShingle Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput Development
Vio, Santiago Adolfo
Ciencias Agrarias
Bioprospecting
Plant growth-promoting bacteria
Biocontrol
Endophytic bacteria
Sustainable horticulture
Lettuce
Tomato
title_short Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput Development
title_full Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput Development
title_fullStr Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput Development
title_full_unstemmed Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput Development
title_sort Exploring the Cultivable Fraction of the Bacterial Microbiome from Tomato Plants for Growth-Promoting and Biocontrol Traits Toward Bioinput Development
dc.creator.none.fl_str_mv Vio, Santiago Adolfo
Paiva González, Karen Belén
Gortari, María Cecilia
Galar, María Lina
Pistorio, Mariano
Luna, María Flavia
author Vio, Santiago Adolfo
author_facet Vio, Santiago Adolfo
Paiva González, Karen Belén
Gortari, María Cecilia
Galar, María Lina
Pistorio, Mariano
Luna, María Flavia
author_role author
author2 Paiva González, Karen Belén
Gortari, María Cecilia
Galar, María Lina
Pistorio, Mariano
Luna, María Flavia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Bioprospecting
Plant growth-promoting bacteria
Biocontrol
Endophytic bacteria
Sustainable horticulture
Lettuce
Tomato
topic Ciencias Agrarias
Bioprospecting
Plant growth-promoting bacteria
Biocontrol
Endophytic bacteria
Sustainable horticulture
Lettuce
Tomato
dc.description.none.fl_txt_mv Plant growth-promoting bacteria (PGPB) represent a sustainable alternative to synthetic inputs in horticultural systems; however, their bioprospecting is hindered by the absence of integrative, performance-oriented selection strategies. In this study, a comprehensive collection of 259 bacterial isolates associated with tomato plants was systematically screened to identify strains with biocontrol and plant growth-promoting potential. Isolates were characterized in vitro for potential plant colonization ability, antifungal activity, and multiple plant growth-promoting mechanisms. These traits were integrated into composite indices and analyzed using multivariate approaches to guide the selection of promising isolates. Selected candidates were subsequently evaluated in vivo for biocontrol and plant growth at both seedling and productive stages, and most isolates exhibited consistent effects. Isolates from the genera Stenotrophomonas, Pseudomonas, and Bacillus reduced fungal disease incidence to 2–9% (control disease: 80%). A Bacillus isolate increased seedling biomass by 54% in lettuce and 38% in tomato. Under productive conditions, lettuce marketable weight increased by 21–37%, whereas tomato yield showed positive but non-significant increases (~21–25%) after inoculation with Pseudomonas or Bacillus isolates. Overall, this work provides a structured framework for PGPB bioprospecting and validation, combining laboratory screening, composite indices, multivariate analyses, and multi-stage in vivo assays under realistic horticultural conditions.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnología y Biología Molecular
description Plant growth-promoting bacteria (PGPB) represent a sustainable alternative to synthetic inputs in horticultural systems; however, their bioprospecting is hindered by the absence of integrative, performance-oriented selection strategies. In this study, a comprehensive collection of 259 bacterial isolates associated with tomato plants was systematically screened to identify strains with biocontrol and plant growth-promoting potential. Isolates were characterized in vitro for potential plant colonization ability, antifungal activity, and multiple plant growth-promoting mechanisms. These traits were integrated into composite indices and analyzed using multivariate approaches to guide the selection of promising isolates. Selected candidates were subsequently evaluated in vivo for biocontrol and plant growth at both seedling and productive stages, and most isolates exhibited consistent effects. Isolates from the genera Stenotrophomonas, Pseudomonas, and Bacillus reduced fungal disease incidence to 2–9% (control disease: 80%). A Bacillus isolate increased seedling biomass by 54% in lettuce and 38% in tomato. Under productive conditions, lettuce marketable weight increased by 21–37%, whereas tomato yield showed positive but non-significant increases (~21–25%) after inoculation with Pseudomonas or Bacillus isolates. Overall, this work provides a structured framework for PGPB bioprospecting and validation, combining laboratory screening, composite indices, multivariate analyses, and multi-stage in vivo assays under realistic horticultural conditions.
publishDate 2026
dc.date.none.fl_str_mv 2026-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/195661
url http://sedici.unlp.edu.ar/handle/10915/195661
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2077-0472
info:eu-repo/semantics/altIdentifier/doi/10.3390/agriculture16050610
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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