Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Meta...

Autores
Schuster, Christina; Ben Gharsa, Haifa; Baró Robaina, Yamilé; Manfrino, Romina Guadalupe; Bobushova, Saikal; Gutiérrez, Alejandra Concepción; López Lastra, Claudia Cristina; Leclerque, Andreas
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Potentially arthropod-pathogenic and plant-associated Metarhizium fungi are of high interest for basic research, biological pest control and plant growth promotion. Unambiguous species delineation enabling the taxonomic assignment of new isolates and the identification of new Metarhizium species is of crucial importance for both research and application. Recently, the new species Metarhizium hybridum and Metarhizium parapingshaense were introduced on the basis of phylogenomic studies. (2) Methods: Neighbor- joining and Bayesian inference-based phylogenetic reconstruction of ribosomal intergenic spacer (rIGS) sequences were used to critically evaluate new species introductions. A species-discriminating diagnostic PCR tool for Metarhizium was adapted to M. hybridum and M. parapingshaense. GenBank database mining was performed to identify cryptic descriptions of the new species. (3) Results: The introduction of M. hybridum and M. parapingshaense was corroborated by rIGS sequence comparison. Data mining revealed cryptic first descriptions of M. hybridum from Canada, China, Colombia, Costa Rica, Cuba, Honduras, Mexico, New Zealand, the USA and the Philippines, and of M. parapingshaense from China, India, Japan, the Philippines and South Korea. (4) Conclusions: Results support the reliability of rIGS as a single taxonomic marker for species-level identification of Metarhizium fungi. Species-discriminating diagnostic PCR was successfully adapted to enable the sequencing-independent identification of the confirmed new species M. hybridum and M. parapingshaense.
Centro de Estudios Parasitológicos y de Vectores
Materia
Ciencias Naturales
Metarhizium hybridum
Metarhizium parapingshaense
Metarhizium anisopliae sensu lato
Metarhizium pingshaense sensu lato
PARB clade
ribosomal intergenic spacer marker (rIGS-ID800)
single-marker phylogeny
sequencing-independent species identification
diagnostic PCR
cryptic species descriptions
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/195361
Acceder
id SEDICI_d689255642edcac048a7f95fef95e9cc
oai_identifier_str oai:sedici.unlp.edu.ar:10915/195361
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaenseSchuster, ChristinaBen Gharsa, HaifaBaró Robaina, YamiléManfrino, Romina GuadalupeBobushova, SaikalGutiérrez, Alejandra ConcepciónLópez Lastra, Claudia CristinaLeclerque, AndreasCiencias NaturalesMetarhizium hybridumMetarhizium parapingshaenseMetarhizium anisopliae sensu latoMetarhizium pingshaense sensu latoPARB claderibosomal intergenic spacer marker (rIGS-ID800)single-marker phylogenysequencing-independent species identificationdiagnostic PCRcryptic species descriptionsBackground: Potentially arthropod-pathogenic and plant-associated Metarhizium fungi are of high interest for basic research, biological pest control and plant growth promotion. Unambiguous species delineation enabling the taxonomic assignment of new isolates and the identification of new Metarhizium species is of crucial importance for both research and application. Recently, the new species Metarhizium hybridum and Metarhizium parapingshaense were introduced on the basis of phylogenomic studies. (2) Methods: Neighbor- joining and Bayesian inference-based phylogenetic reconstruction of ribosomal intergenic spacer (rIGS) sequences were used to critically evaluate new species introductions. A species-discriminating diagnostic PCR tool for Metarhizium was adapted to M. hybridum and M. parapingshaense. GenBank database mining was performed to identify cryptic descriptions of the new species. (3) Results: The introduction of M. hybridum and M. parapingshaense was corroborated by rIGS sequence comparison. Data mining revealed cryptic first descriptions of M. hybridum from Canada, China, Colombia, Costa Rica, Cuba, Honduras, Mexico, New Zealand, the USA and the Philippines, and of M. parapingshaense from China, India, Japan, the Philippines and South Korea. (4) Conclusions: Results support the reliability of rIGS as a single taxonomic marker for species-level identification of Metarhizium fungi. Species-discriminating diagnostic PCR was successfully adapted to enable the sequencing-independent identification of the confirmed new species M. hybridum and M. parapingshaense.Centro de Estudios Parasitológicos y de Vectores2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/195361enginfo:eu-repo/semantics/altIdentifier/issn/2309-608Xinfo:eu-repo/semantics/altIdentifier/doi/10.3390/jof12040272info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2026-06-23T11:17:02Zoai:sedici.unlp.edu.ar:10915/195361Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292026-06-23 11:17:02.67SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
spellingShingle Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
Schuster, Christina
Ciencias Naturales
Metarhizium hybridum
Metarhizium parapingshaense
Metarhizium anisopliae sensu lato
Metarhizium pingshaense sensu lato
PARB clade
ribosomal intergenic spacer marker (rIGS-ID800)
single-marker phylogeny
sequencing-independent species identification
diagnostic PCR
cryptic species descriptions
title_short Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title_full Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title_fullStr Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title_full_unstemmed Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title_sort Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
dc.creator.none.fl_str_mv Schuster, Christina
Ben Gharsa, Haifa
Baró Robaina, Yamilé
Manfrino, Romina Guadalupe
Bobushova, Saikal
Gutiérrez, Alejandra Concepción
López Lastra, Claudia Cristina
Leclerque, Andreas
author Schuster, Christina
author_facet Schuster, Christina
Ben Gharsa, Haifa
Baró Robaina, Yamilé
Manfrino, Romina Guadalupe
Bobushova, Saikal
Gutiérrez, Alejandra Concepción
López Lastra, Claudia Cristina
Leclerque, Andreas
author_role author
author2 Ben Gharsa, Haifa
Baró Robaina, Yamilé
Manfrino, Romina Guadalupe
Bobushova, Saikal
Gutiérrez, Alejandra Concepción
López Lastra, Claudia Cristina
Leclerque, Andreas
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Metarhizium hybridum
Metarhizium parapingshaense
Metarhizium anisopliae sensu lato
Metarhizium pingshaense sensu lato
PARB clade
ribosomal intergenic spacer marker (rIGS-ID800)
single-marker phylogeny
sequencing-independent species identification
diagnostic PCR
cryptic species descriptions
topic Ciencias Naturales
Metarhizium hybridum
Metarhizium parapingshaense
Metarhizium anisopliae sensu lato
Metarhizium pingshaense sensu lato
PARB clade
ribosomal intergenic spacer marker (rIGS-ID800)
single-marker phylogeny
sequencing-independent species identification
diagnostic PCR
cryptic species descriptions
dc.description.none.fl_txt_mv Background: Potentially arthropod-pathogenic and plant-associated Metarhizium fungi are of high interest for basic research, biological pest control and plant growth promotion. Unambiguous species delineation enabling the taxonomic assignment of new isolates and the identification of new Metarhizium species is of crucial importance for both research and application. Recently, the new species Metarhizium hybridum and Metarhizium parapingshaense were introduced on the basis of phylogenomic studies. (2) Methods: Neighbor- joining and Bayesian inference-based phylogenetic reconstruction of ribosomal intergenic spacer (rIGS) sequences were used to critically evaluate new species introductions. A species-discriminating diagnostic PCR tool for Metarhizium was adapted to M. hybridum and M. parapingshaense. GenBank database mining was performed to identify cryptic descriptions of the new species. (3) Results: The introduction of M. hybridum and M. parapingshaense was corroborated by rIGS sequence comparison. Data mining revealed cryptic first descriptions of M. hybridum from Canada, China, Colombia, Costa Rica, Cuba, Honduras, Mexico, New Zealand, the USA and the Philippines, and of M. parapingshaense from China, India, Japan, the Philippines and South Korea. (4) Conclusions: Results support the reliability of rIGS as a single taxonomic marker for species-level identification of Metarhizium fungi. Species-discriminating diagnostic PCR was successfully adapted to enable the sequencing-independent identification of the confirmed new species M. hybridum and M. parapingshaense.
Centro de Estudios Parasitológicos y de Vectores
description Background: Potentially arthropod-pathogenic and plant-associated Metarhizium fungi are of high interest for basic research, biological pest control and plant growth promotion. Unambiguous species delineation enabling the taxonomic assignment of new isolates and the identification of new Metarhizium species is of crucial importance for both research and application. Recently, the new species Metarhizium hybridum and Metarhizium parapingshaense were introduced on the basis of phylogenomic studies. (2) Methods: Neighbor- joining and Bayesian inference-based phylogenetic reconstruction of ribosomal intergenic spacer (rIGS) sequences were used to critically evaluate new species introductions. A species-discriminating diagnostic PCR tool for Metarhizium was adapted to M. hybridum and M. parapingshaense. GenBank database mining was performed to identify cryptic descriptions of the new species. (3) Results: The introduction of M. hybridum and M. parapingshaense was corroborated by rIGS sequence comparison. Data mining revealed cryptic first descriptions of M. hybridum from Canada, China, Colombia, Costa Rica, Cuba, Honduras, Mexico, New Zealand, the USA and the Philippines, and of M. parapingshaense from China, India, Japan, the Philippines and South Korea. (4) Conclusions: Results support the reliability of rIGS as a single taxonomic marker for species-level identification of Metarhizium fungi. Species-discriminating diagnostic PCR was successfully adapted to enable the sequencing-independent identification of the confirmed new species M. hybridum and M. parapingshaense.
publishDate 2026
dc.date.none.fl_str_mv 2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/195361
url http://sedici.unlp.edu.ar/handle/10915/195361
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2309-608X
info:eu-repo/semantics/altIdentifier/doi/10.3390/jof12040272
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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