Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production

Autores
Irazoqui, José Matías; Mainez, María Esperanza; Valenti, Alejandro; Santiago, Gonzalo Manuel; Paez, Roxana; Amadio, Ariel Fernando; Eberhardt, Maria Florencia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyhydroxyalkanoates are a microbial storage polymer that presents similar characteristics to plastics, therefore are usually referred to as bioplastics. The key enzyme involved in the synthesis of polyhydroxyalkanoates is called PHA synthase, but it is always forming a synthesis cassette of three to five enzymes.In this work, we present a novel class III polyhydroxyalkanoate synthesis cassette identified using a sequence-based metagenomics approach. From two metagenomes from dairy industries stabilization ponds, we found four complete synthesis cassettes. One of them was successfully cloned into Escherichia coli CGSC: 6576, a bacterial strain able to grow in lactose and whey permeate, a by-product of dairy industries that represent an environmental risk.This recombinant strain grown in lactose was able to accumulate nearly 40% of dry weight as insoluble material identified by Fourier Transform Infrared Spectroscopy (FT-IR), Gas Chromatography with Flame Ionization Detection (GC-FID) and characterized by Nuclear Magnetic Resonance (NMR) as poly(3-hydroxybutyrate), one of the main polyhydroxyalkanoates commercially used.This research presents the complete process, from the identification of novel enzymes from uncultured microorganisms to the synthesis at laboratory scale of compounds of industrial interest.
Fil: Irazoqui, José Matías. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Mainez, María Esperanza. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Valenti, Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Santiago, Gonzalo Manuel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Paez, Roxana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Amadio, Ariel Fernando. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Eberhardt, Maria Florencia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Materia
Polyhydroxyalcanoates
metagenomics
Escherichia coli
bioplastics
lactose
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291295
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291295
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer ProductionIrazoqui, José MatíasMainez, María EsperanzaValenti, AlejandroSantiago, Gonzalo ManuelPaez, RoxanaAmadio, Ariel FernandoEberhardt, Maria FlorenciaPolyhydroxyalcanoatesmetagenomicsEscherichia colibioplasticslactosehttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Polyhydroxyalkanoates are a microbial storage polymer that presents similar characteristics to plastics, therefore are usually referred to as bioplastics. The key enzyme involved in the synthesis of polyhydroxyalkanoates is called PHA synthase, but it is always forming a synthesis cassette of three to five enzymes.In this work, we present a novel class III polyhydroxyalkanoate synthesis cassette identified using a sequence-based metagenomics approach. From two metagenomes from dairy industries stabilization ponds, we found four complete synthesis cassettes. One of them was successfully cloned into Escherichia coli CGSC: 6576, a bacterial strain able to grow in lactose and whey permeate, a by-product of dairy industries that represent an environmental risk.This recombinant strain grown in lactose was able to accumulate nearly 40% of dry weight as insoluble material identified by Fourier Transform Infrared Spectroscopy (FT-IR), Gas Chromatography with Flame Ionization Detection (GC-FID) and characterized by Nuclear Magnetic Resonance (NMR) as poly(3-hydroxybutyrate), one of the main polyhydroxyalkanoates commercially used.This research presents the complete process, from the identification of novel enzymes from uncultured microorganisms to the synthesis at laboratory scale of compounds of industrial interest.Fil: Irazoqui, José Matías. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Mainez, María Esperanza. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Valenti, Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Santiago, Gonzalo Manuel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Paez, Roxana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Amadio, Ariel Fernando. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Eberhardt, Maria Florencia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaSpringer/Plenum Publishers2025-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291295Irazoqui, José Matías; Mainez, María Esperanza; Valenti, Alejandro; Santiago, Gonzalo Manuel; Paez, Roxana; et al.; Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production; Springer/Plenum Publishers; Journal of Polymers and the Environment; 34; 1; 12-2025; 1-271566-2543CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10924-025-03756-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:10:03Zoai:ri.conicet.gov.ar:11336/291295instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:10:04.278CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production
title Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production
spellingShingle Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production
Irazoqui, José Matías
Polyhydroxyalcanoates
metagenomics
Escherichia coli
bioplastics
lactose
title_short Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production
title_full Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production
title_fullStr Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production
title_full_unstemmed Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production
title_sort Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production
dc.creator.none.fl_str_mv Irazoqui, José Matías
Mainez, María Esperanza
Valenti, Alejandro
Santiago, Gonzalo Manuel
Paez, Roxana
Amadio, Ariel Fernando
Eberhardt, Maria Florencia
author Irazoqui, José Matías
author_facet Irazoqui, José Matías
Mainez, María Esperanza
Valenti, Alejandro
Santiago, Gonzalo Manuel
Paez, Roxana
Amadio, Ariel Fernando
Eberhardt, Maria Florencia
author_role author
author2 Mainez, María Esperanza
Valenti, Alejandro
Santiago, Gonzalo Manuel
Paez, Roxana
Amadio, Ariel Fernando
Eberhardt, Maria Florencia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Polyhydroxyalcanoates
metagenomics
Escherichia coli
bioplastics
lactose
topic Polyhydroxyalcanoates
metagenomics
Escherichia coli
bioplastics
lactose
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Polyhydroxyalkanoates are a microbial storage polymer that presents similar characteristics to plastics, therefore are usually referred to as bioplastics. The key enzyme involved in the synthesis of polyhydroxyalkanoates is called PHA synthase, but it is always forming a synthesis cassette of three to five enzymes.In this work, we present a novel class III polyhydroxyalkanoate synthesis cassette identified using a sequence-based metagenomics approach. From two metagenomes from dairy industries stabilization ponds, we found four complete synthesis cassettes. One of them was successfully cloned into Escherichia coli CGSC: 6576, a bacterial strain able to grow in lactose and whey permeate, a by-product of dairy industries that represent an environmental risk.This recombinant strain grown in lactose was able to accumulate nearly 40% of dry weight as insoluble material identified by Fourier Transform Infrared Spectroscopy (FT-IR), Gas Chromatography with Flame Ionization Detection (GC-FID) and characterized by Nuclear Magnetic Resonance (NMR) as poly(3-hydroxybutyrate), one of the main polyhydroxyalkanoates commercially used.This research presents the complete process, from the identification of novel enzymes from uncultured microorganisms to the synthesis at laboratory scale of compounds of industrial interest.
Fil: Irazoqui, José Matías. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Mainez, María Esperanza. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Valenti, Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Santiago, Gonzalo Manuel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Paez, Roxana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Amadio, Ariel Fernando. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Eberhardt, Maria Florencia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
description Polyhydroxyalkanoates are a microbial storage polymer that presents similar characteristics to plastics, therefore are usually referred to as bioplastics. The key enzyme involved in the synthesis of polyhydroxyalkanoates is called PHA synthase, but it is always forming a synthesis cassette of three to five enzymes.In this work, we present a novel class III polyhydroxyalkanoate synthesis cassette identified using a sequence-based metagenomics approach. From two metagenomes from dairy industries stabilization ponds, we found four complete synthesis cassettes. One of them was successfully cloned into Escherichia coli CGSC: 6576, a bacterial strain able to grow in lactose and whey permeate, a by-product of dairy industries that represent an environmental risk.This recombinant strain grown in lactose was able to accumulate nearly 40% of dry weight as insoluble material identified by Fourier Transform Infrared Spectroscopy (FT-IR), Gas Chromatography with Flame Ionization Detection (GC-FID) and characterized by Nuclear Magnetic Resonance (NMR) as poly(3-hydroxybutyrate), one of the main polyhydroxyalkanoates commercially used.This research presents the complete process, from the identification of novel enzymes from uncultured microorganisms to the synthesis at laboratory scale of compounds of industrial interest.
publishDate 2025
dc.date.none.fl_str_mv 2025-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291295
Irazoqui, José Matías; Mainez, María Esperanza; Valenti, Alejandro; Santiago, Gonzalo Manuel; Paez, Roxana; et al.; Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production; Springer/Plenum Publishers; Journal of Polymers and the Environment; 34; 1; 12-2025; 1-27
1566-2543
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291295
identifier_str_mv Irazoqui, José Matías; Mainez, María Esperanza; Valenti, Alejandro; Santiago, Gonzalo Manuel; Paez, Roxana; et al.; Evaluation of a Class III PHA Synthase Identified in Dairy Pond Metagenomes for Biopolymer Production; Springer/Plenum Publishers; Journal of Polymers and the Environment; 34; 1; 12-2025; 1-27
1566-2543
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10924-025-03756-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer/Plenum Publishers
publisher.none.fl_str_mv Springer/Plenum Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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