Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary study

Autores
Pila, Andrea Natalia; Rojo de Benito, Elena Maria; Filipigh, Ángel Alejandro; Dagnino, Eliana Paola; Bolado Rodríguez, Silvia
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since the middle of the 20TH century, the introduction of plastics in the global market has produced a noticeable improvement in the comfort and the quality of life of humanity, being present not only in almost all aspects of our daily life, but also in the industry. Its versatility and humanity’s astonishing technological development throughout the decades has deemed its application essential in almost every aspect. Even though the exact number of plastic wastes disposed in unregulated landfills or in nature remains unknown, it is estimated that approximately 5 to 13 million metric tons are disposed in the ocean around the globe. In that sense, many significant breakthroughs related to the production of biodegradable polymers from removable resources in the biotechnological field have been achieved, aiming for Cero Waste and Net-Cero CO2 Emissions objectives. The polyesters produced by bacteria represent an specific type of biopolymer generated by microorganisms. Within the group, we can find Polyhydroxyalcanoates (PHAs), which can be synthesized by microorganisms as an energy storage mechanism. PHAs have presented promising results for its usage in the plastic industry, but its high production costs limit its production and utilization. For that reason, the main goal of this work is to evaluate the utilization of waste microalgal biomass as a carbon source for Paracoccus denitrificants PHAs production. Scenedesmus almeriensis based biomass grown on photo-bioreactor used for pig manure wastewater remediation was used for this analysis. Different types of hydrolysis (acid, alkaline and enzymatic) were used for recovering of carbon and nutrients from waste biomass. Hydrolysates, containing released monosaccharides and organic acids, among others components, were used as substrate for the production of PHAs. Paracoccus denitrificants were cultivated in minimum medium using the hydrolyzed as the only carbon source. The cellular growth was followed measuring DO 600 nm. The results show that both, acid and enzymatic hydrolyzed, are appropriate for P denitrificans growth, while alkaline hydrolyzed is not a suitable carbon source for the growth of P denitrificans and was discarted. After the cellular growth, the supernatant was separated by centrifugation (10.000 rpm, 10 minutes) and the total content of PHAs in the pellet was determinated by extraction with chloroform and measuring by Gas Chromatography coupled with a Mass detector. P denitrificans cultivated in acid hydrolyzed resulted in 4.99 mg g-1, while the ones cultivated in enzymatic hydrolyzed resulted in a lower yield of 3.61 mg g-1. All the results were expressed in dry basis. The results show that hydrolyzed waste microalgae biomass can be used as carbon source for the productions of PHAs using Paracoccus denitrificans.
Fil: Pila, Andrea Natalia. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.
Fil: Rojo de Benito, Elena María, Universidad de Valladolid. Departamento de Ingeniería Química y Tecnología del Medio Ambiente. Instituto de Procesos Sostenibles; España.
Fil: Filipigh, Ángel Alejandro. Universidad de Valladolid. Departamento de Ingeniería Química y Tecnología del Medio Ambiente. Instituto de Procesos Sostenibles; España.
Fil: Dagnino, Eliana Paola. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.
Fil: Bolado Rodríguez, Silvia. Universidad de Valladolid. Departamento de Ingeniería Química y Tecnología del Medio Ambiente. Instituto de Procesos Sostenibles; España.
Peer Reviewed
Materia
bioplastics
hydrolysates
microalgal
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2024-03-25T22:20:40Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/10072
Acceder
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network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary studyPila, Andrea NataliaRojo de Benito, Elena MariaFilipigh, Ángel AlejandroDagnino, Eliana PaolaBolado Rodríguez, SilviabioplasticshydrolysatesmicroalgalSince the middle of the 20TH century, the introduction of plastics in the global market has produced a noticeable improvement in the comfort and the quality of life of humanity, being present not only in almost all aspects of our daily life, but also in the industry. Its versatility and humanity’s astonishing technological development throughout the decades has deemed its application essential in almost every aspect. Even though the exact number of plastic wastes disposed in unregulated landfills or in nature remains unknown, it is estimated that approximately 5 to 13 million metric tons are disposed in the ocean around the globe. In that sense, many significant breakthroughs related to the production of biodegradable polymers from removable resources in the biotechnological field have been achieved, aiming for Cero Waste and Net-Cero CO2 Emissions objectives. The polyesters produced by bacteria represent an specific type of biopolymer generated by microorganisms. Within the group, we can find Polyhydroxyalcanoates (PHAs), which can be synthesized by microorganisms as an energy storage mechanism. PHAs have presented promising results for its usage in the plastic industry, but its high production costs limit its production and utilization. For that reason, the main goal of this work is to evaluate the utilization of waste microalgal biomass as a carbon source for Paracoccus denitrificants PHAs production. Scenedesmus almeriensis based biomass grown on photo-bioreactor used for pig manure wastewater remediation was used for this analysis. Different types of hydrolysis (acid, alkaline and enzymatic) were used for recovering of carbon and nutrients from waste biomass. Hydrolysates, containing released monosaccharides and organic acids, among others components, were used as substrate for the production of PHAs. Paracoccus denitrificants were cultivated in minimum medium using the hydrolyzed as the only carbon source. The cellular growth was followed measuring DO 600 nm. The results show that both, acid and enzymatic hydrolyzed, are appropriate for P denitrificans growth, while alkaline hydrolyzed is not a suitable carbon source for the growth of P denitrificans and was discarted. After the cellular growth, the supernatant was separated by centrifugation (10.000 rpm, 10 minutes) and the total content of PHAs in the pellet was determinated by extraction with chloroform and measuring by Gas Chromatography coupled with a Mass detector. P denitrificans cultivated in acid hydrolyzed resulted in 4.99 mg g-1, while the ones cultivated in enzymatic hydrolyzed resulted in a lower yield of 3.61 mg g-1. All the results were expressed in dry basis. The results show that hydrolyzed waste microalgae biomass can be used as carbon source for the productions of PHAs using Paracoccus denitrificans.Fil: Pila, Andrea Natalia. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.Fil: Rojo de Benito, Elena María, Universidad de Valladolid. Departamento de Ingeniería Química y Tecnología del Medio Ambiente. Instituto de Procesos Sostenibles; España.Fil: Filipigh, Ángel Alejandro. Universidad de Valladolid. Departamento de Ingeniería Química y Tecnología del Medio Ambiente. Instituto de Procesos Sostenibles; España.Fil: Dagnino, Eliana Paola. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.Fil: Bolado Rodríguez, Silvia. Universidad de Valladolid. Departamento de Ingeniería Química y Tecnología del Medio Ambiente. Instituto de Procesos Sostenibles; España.Peer Reviewed2024-03-25T22:20:40Z2024-03-25T22:20:40Z2023-06-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articulopdfapplication/pdf11th World Congress of Chemical Engineering WCCE112953-5565http://hdl.handle.net/20.500.12272/10072engBiorrefinería de biomasa microalgal: Obtención de colorantes y bioplásticos a partir de microalgas autóctonas producidas en el tratamiento de efluentes localesPATCARE0008065TCinfo:eu-repo/semantics/openAccess2024-03-25T22:20:40Zhttp://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 4.0 InternacionalAcceso abiertoreponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:45:35Zoai:ria.utn.edu.ar:20.500.12272/10072instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:45:36.267Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary study
title Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary study
spellingShingle Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary study
Pila, Andrea Natalia
bioplastics
hydrolysates
microalgal
title_short Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary study
title_full Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary study
title_fullStr Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary study
title_full_unstemmed Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary study
title_sort Use of hydrolysates from waste microagal biomass for bioplastics production by Paracoccus denitrificans : a preliminary study
dc.creator.none.fl_str_mv Pila, Andrea Natalia
Rojo de Benito, Elena Maria
Filipigh, Ángel Alejandro
Dagnino, Eliana Paola
Bolado Rodríguez, Silvia
author Pila, Andrea Natalia
author_facet Pila, Andrea Natalia
Rojo de Benito, Elena Maria
Filipigh, Ángel Alejandro
Dagnino, Eliana Paola
Bolado Rodríguez, Silvia
author_role author
author2 Rojo de Benito, Elena Maria
Filipigh, Ángel Alejandro
Dagnino, Eliana Paola
Bolado Rodríguez, Silvia
author2_role author
author
author
author
dc.subject.none.fl_str_mv bioplastics
hydrolysates
microalgal
topic bioplastics
hydrolysates
microalgal
dc.description.none.fl_txt_mv Since the middle of the 20TH century, the introduction of plastics in the global market has produced a noticeable improvement in the comfort and the quality of life of humanity, being present not only in almost all aspects of our daily life, but also in the industry. Its versatility and humanity’s astonishing technological development throughout the decades has deemed its application essential in almost every aspect. Even though the exact number of plastic wastes disposed in unregulated landfills or in nature remains unknown, it is estimated that approximately 5 to 13 million metric tons are disposed in the ocean around the globe. In that sense, many significant breakthroughs related to the production of biodegradable polymers from removable resources in the biotechnological field have been achieved, aiming for Cero Waste and Net-Cero CO2 Emissions objectives. The polyesters produced by bacteria represent an specific type of biopolymer generated by microorganisms. Within the group, we can find Polyhydroxyalcanoates (PHAs), which can be synthesized by microorganisms as an energy storage mechanism. PHAs have presented promising results for its usage in the plastic industry, but its high production costs limit its production and utilization. For that reason, the main goal of this work is to evaluate the utilization of waste microalgal biomass as a carbon source for Paracoccus denitrificants PHAs production. Scenedesmus almeriensis based biomass grown on photo-bioreactor used for pig manure wastewater remediation was used for this analysis. Different types of hydrolysis (acid, alkaline and enzymatic) were used for recovering of carbon and nutrients from waste biomass. Hydrolysates, containing released monosaccharides and organic acids, among others components, were used as substrate for the production of PHAs. Paracoccus denitrificants were cultivated in minimum medium using the hydrolyzed as the only carbon source. The cellular growth was followed measuring DO 600 nm. The results show that both, acid and enzymatic hydrolyzed, are appropriate for P denitrificans growth, while alkaline hydrolyzed is not a suitable carbon source for the growth of P denitrificans and was discarted. After the cellular growth, the supernatant was separated by centrifugation (10.000 rpm, 10 minutes) and the total content of PHAs in the pellet was determinated by extraction with chloroform and measuring by Gas Chromatography coupled with a Mass detector. P denitrificans cultivated in acid hydrolyzed resulted in 4.99 mg g-1, while the ones cultivated in enzymatic hydrolyzed resulted in a lower yield of 3.61 mg g-1. All the results were expressed in dry basis. The results show that hydrolyzed waste microalgae biomass can be used as carbon source for the productions of PHAs using Paracoccus denitrificans.
Fil: Pila, Andrea Natalia. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.
Fil: Rojo de Benito, Elena María, Universidad de Valladolid. Departamento de Ingeniería Química y Tecnología del Medio Ambiente. Instituto de Procesos Sostenibles; España.
Fil: Filipigh, Ángel Alejandro. Universidad de Valladolid. Departamento de Ingeniería Química y Tecnología del Medio Ambiente. Instituto de Procesos Sostenibles; España.
Fil: Dagnino, Eliana Paola. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.
Fil: Bolado Rodríguez, Silvia. Universidad de Valladolid. Departamento de Ingeniería Química y Tecnología del Medio Ambiente. Instituto de Procesos Sostenibles; España.
Peer Reviewed
description Since the middle of the 20TH century, the introduction of plastics in the global market has produced a noticeable improvement in the comfort and the quality of life of humanity, being present not only in almost all aspects of our daily life, but also in the industry. Its versatility and humanity’s astonishing technological development throughout the decades has deemed its application essential in almost every aspect. Even though the exact number of plastic wastes disposed in unregulated landfills or in nature remains unknown, it is estimated that approximately 5 to 13 million metric tons are disposed in the ocean around the globe. In that sense, many significant breakthroughs related to the production of biodegradable polymers from removable resources in the biotechnological field have been achieved, aiming for Cero Waste and Net-Cero CO2 Emissions objectives. The polyesters produced by bacteria represent an specific type of biopolymer generated by microorganisms. Within the group, we can find Polyhydroxyalcanoates (PHAs), which can be synthesized by microorganisms as an energy storage mechanism. PHAs have presented promising results for its usage in the plastic industry, but its high production costs limit its production and utilization. For that reason, the main goal of this work is to evaluate the utilization of waste microalgal biomass as a carbon source for Paracoccus denitrificants PHAs production. Scenedesmus almeriensis based biomass grown on photo-bioreactor used for pig manure wastewater remediation was used for this analysis. Different types of hydrolysis (acid, alkaline and enzymatic) were used for recovering of carbon and nutrients from waste biomass. Hydrolysates, containing released monosaccharides and organic acids, among others components, were used as substrate for the production of PHAs. Paracoccus denitrificants were cultivated in minimum medium using the hydrolyzed as the only carbon source. The cellular growth was followed measuring DO 600 nm. The results show that both, acid and enzymatic hydrolyzed, are appropriate for P denitrificans growth, while alkaline hydrolyzed is not a suitable carbon source for the growth of P denitrificans and was discarted. After the cellular growth, the supernatant was separated by centrifugation (10.000 rpm, 10 minutes) and the total content of PHAs in the pellet was determinated by extraction with chloroform and measuring by Gas Chromatography coupled with a Mass detector. P denitrificans cultivated in acid hydrolyzed resulted in 4.99 mg g-1, while the ones cultivated in enzymatic hydrolyzed resulted in a lower yield of 3.61 mg g-1. All the results were expressed in dry basis. The results show that hydrolyzed waste microalgae biomass can be used as carbon source for the productions of PHAs using Paracoccus denitrificans.
publishDate 2023
dc.date.none.fl_str_mv 2023-06-08
2024-03-25T22:20:40Z
2024-03-25T22:20:40Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 11th World Congress of Chemical Engineering WCCE11
2953-5565
http://hdl.handle.net/20.500.12272/10072
identifier_str_mv 11th World Congress of Chemical Engineering WCCE11
2953-5565
url http://hdl.handle.net/20.500.12272/10072
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biorrefinería de biomasa microalgal: Obtención de colorantes y bioplásticos a partir de microalgas autóctonas producidas en el tratamiento de efluentes locales
PATCARE0008065TC
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2024-03-25T22:20:40Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Acceso abierto
eu_rights_str_mv openAccess
rights_invalid_str_mv 2024-03-25T22:20:40Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Acceso abierto
dc.format.none.fl_str_mv pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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score 13.24418

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