Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megaterium

Autores
Pila, Andrea Natalia; Perezlindo, Victoria Agostina; Dagnino, Eliana Paola
Año de publicación
2026
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Plastic consumption is a widely discussed topic and one of the concerns of humanity due to its contribution to environmental pollution and the use of fossil resources. One of the most promising alternatives for replacing conventional plastic is the use of biopolymers produced by microorganisms. Among these alternatives, polyhydroxyalkanoates (PHAs) are one of the materials that have gained the most importance in recent years. Agro-forestry and industrial activities generate enormous quantities of waste annually constituting one of the largest waste streams worldwide one of the largest categories globally. Rice husk (RH) is a lignocellulosic residue that poses a challenge for the agro-industry: it represents about 20% of the total weight of the rice harvest and is often stockpiled in large quantities or discarded. Nevertheless, RH consists mainly of carbohydrates that can serve as a raw material for the production of high value-added products. The aim of this study was to evaluate the use of RH hydrolysate as a substrate for PHA production and to examine the effect of selected variables. The microorganism selected was Bacillus megaterium, a PHA-producing bacterium under conditions of carbon excess and nitrogen limitation. RH was hydrolyzed with formic acid with chloridric acid as enhanced to maximize monosaccharide (glucose, xylose) release and minimize furfural formation, which inhibits bacterial growth. For the growth and PHA-production stages, the influence of hydrolysate concentration in the medium (25, 50, and 75%, v/v) was assessed. Sugar and furfural concentrations were determined by HPLC; bacterial growth was monitored by optical density at 600 nm (OD₆₀₀); and PHAs were quantified by chloroform extraction, sulfuric-acid conversion, and spectrophotometric determination at 240 nm. The greatest PHA concentration (1.78 g PHA per 100 g dry biomass) was obtained using the 120-min hydrolysate diluted to 50%. This lower yield can be attributed to the complexity of the real residue and to inhibitory compounds formed during hydrolysis, such as furfural. In conclusion, rice husk represents a practical substrate for bioplastic synthesis, but further work is required to refine operating conditions and boost PHA yields.
Fil: Perezlindo, Victoria Agostina. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.
Fil: Pila, Andrea Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional del Nordeste. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Dagnino, Eliana Paola. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.
Fil: Dagnino, Eliana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Materia
rice huck
biorefinery
microorganisms
PHAs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Attribution-NonCommercial-ShareAlike 4.0 International
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/14918
Acceder
id RIAUTN_5684dfa61da31e137cec1005633630c0
oai_identifier_str oai:ria.utn.edu.ar:20.500.12272/14918
network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megateriumUtilización de hidrolizados de biomasa lignocelulósica para la producción de bioplásticos por Bacillus megateriumPila, Andrea NataliaPerezlindo, Victoria AgostinaDagnino, Eliana Paolarice huckbiorefinerymicroorganismsPHAsPlastic consumption is a widely discussed topic and one of the concerns of humanity due to its contribution to environmental pollution and the use of fossil resources. One of the most promising alternatives for replacing conventional plastic is the use of biopolymers produced by microorganisms. Among these alternatives, polyhydroxyalkanoates (PHAs) are one of the materials that have gained the most importance in recent years. Agro-forestry and industrial activities generate enormous quantities of waste annually constituting one of the largest waste streams worldwide one of the largest categories globally. Rice husk (RH) is a lignocellulosic residue that poses a challenge for the agro-industry: it represents about 20% of the total weight of the rice harvest and is often stockpiled in large quantities or discarded. Nevertheless, RH consists mainly of carbohydrates that can serve as a raw material for the production of high value-added products. The aim of this study was to evaluate the use of RH hydrolysate as a substrate for PHA production and to examine the effect of selected variables. The microorganism selected was Bacillus megaterium, a PHA-producing bacterium under conditions of carbon excess and nitrogen limitation. RH was hydrolyzed with formic acid with chloridric acid as enhanced to maximize monosaccharide (glucose, xylose) release and minimize furfural formation, which inhibits bacterial growth. For the growth and PHA-production stages, the influence of hydrolysate concentration in the medium (25, 50, and 75%, v/v) was assessed. Sugar and furfural concentrations were determined by HPLC; bacterial growth was monitored by optical density at 600 nm (OD₆₀₀); and PHAs were quantified by chloroform extraction, sulfuric-acid conversion, and spectrophotometric determination at 240 nm. The greatest PHA concentration (1.78 g PHA per 100 g dry biomass) was obtained using the 120-min hydrolysate diluted to 50%. This lower yield can be attributed to the complexity of the real residue and to inhibitory compounds formed during hydrolysis, such as furfural. In conclusion, rice husk represents a practical substrate for bioplastic synthesis, but further work is required to refine operating conditions and boost PHA yields.Fil: Perezlindo, Victoria Agostina. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.Fil: Pila, Andrea Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional del Nordeste. Instituto de Modelado e Innovación Tecnológica; Argentina.Fil: Dagnino, Eliana Paola. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.Fil: Dagnino, Eliana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.2026-05-06T23:16:29Z2026-08-08info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfV Simposio de Residuos Agropecuarios y Agroindustriales; IX International Symposium on Agricultural and Agroindustrial Waste Management978-65-88155-15-8https://simposioderesiduos.com.ar/wp-content/uploads/2026/08/ANAIS-VIII-SIGERA_2025.pdfhttps://hdl.handle.net/20.500.12272/14918engValorización de residuos lignocelulósicos para la obtención de polímeros biodegradablesPATCRE0010068TCinfo:eu-repo/semantics/openAccessAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/Acceso abiertoreponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-10-01T12:00:11Zoai:ria.utn.edu.ar:20.500.12272/14918instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-10-01 12:00:12.326Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megaterium
Utilización de hidrolizados de biomasa lignocelulósica para la producción de bioplásticos por Bacillus megaterium
title Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megaterium
spellingShingle Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megaterium
Pila, Andrea Natalia
rice huck
biorefinery
microorganisms
PHAs
title_short Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megaterium
title_full Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megaterium
title_fullStr Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megaterium
title_full_unstemmed Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megaterium
title_sort Use of hydrolysates from lignocellulosic biomass for bioplastics production by Bacillus megaterium
dc.creator.none.fl_str_mv Pila, Andrea Natalia
Perezlindo, Victoria Agostina
Dagnino, Eliana Paola
author Pila, Andrea Natalia
author_facet Pila, Andrea Natalia
Perezlindo, Victoria Agostina
Dagnino, Eliana Paola
author_role author
author2 Perezlindo, Victoria Agostina
Dagnino, Eliana Paola
author2_role author
author
dc.subject.none.fl_str_mv rice huck
biorefinery
microorganisms
PHAs
topic rice huck
biorefinery
microorganisms
PHAs
dc.description.none.fl_txt_mv Plastic consumption is a widely discussed topic and one of the concerns of humanity due to its contribution to environmental pollution and the use of fossil resources. One of the most promising alternatives for replacing conventional plastic is the use of biopolymers produced by microorganisms. Among these alternatives, polyhydroxyalkanoates (PHAs) are one of the materials that have gained the most importance in recent years. Agro-forestry and industrial activities generate enormous quantities of waste annually constituting one of the largest waste streams worldwide one of the largest categories globally. Rice husk (RH) is a lignocellulosic residue that poses a challenge for the agro-industry: it represents about 20% of the total weight of the rice harvest and is often stockpiled in large quantities or discarded. Nevertheless, RH consists mainly of carbohydrates that can serve as a raw material for the production of high value-added products. The aim of this study was to evaluate the use of RH hydrolysate as a substrate for PHA production and to examine the effect of selected variables. The microorganism selected was Bacillus megaterium, a PHA-producing bacterium under conditions of carbon excess and nitrogen limitation. RH was hydrolyzed with formic acid with chloridric acid as enhanced to maximize monosaccharide (glucose, xylose) release and minimize furfural formation, which inhibits bacterial growth. For the growth and PHA-production stages, the influence of hydrolysate concentration in the medium (25, 50, and 75%, v/v) was assessed. Sugar and furfural concentrations were determined by HPLC; bacterial growth was monitored by optical density at 600 nm (OD₆₀₀); and PHAs were quantified by chloroform extraction, sulfuric-acid conversion, and spectrophotometric determination at 240 nm. The greatest PHA concentration (1.78 g PHA per 100 g dry biomass) was obtained using the 120-min hydrolysate diluted to 50%. This lower yield can be attributed to the complexity of the real residue and to inhibitory compounds formed during hydrolysis, such as furfural. In conclusion, rice husk represents a practical substrate for bioplastic synthesis, but further work is required to refine operating conditions and boost PHA yields.
Fil: Perezlindo, Victoria Agostina. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.
Fil: Pila, Andrea Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional del Nordeste. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Dagnino, Eliana Paola. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Centro de Investigación en Química Orgánica Biológica; Argentina.
Fil: Dagnino, Eliana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
description Plastic consumption is a widely discussed topic and one of the concerns of humanity due to its contribution to environmental pollution and the use of fossil resources. One of the most promising alternatives for replacing conventional plastic is the use of biopolymers produced by microorganisms. Among these alternatives, polyhydroxyalkanoates (PHAs) are one of the materials that have gained the most importance in recent years. Agro-forestry and industrial activities generate enormous quantities of waste annually constituting one of the largest waste streams worldwide one of the largest categories globally. Rice husk (RH) is a lignocellulosic residue that poses a challenge for the agro-industry: it represents about 20% of the total weight of the rice harvest and is often stockpiled in large quantities or discarded. Nevertheless, RH consists mainly of carbohydrates that can serve as a raw material for the production of high value-added products. The aim of this study was to evaluate the use of RH hydrolysate as a substrate for PHA production and to examine the effect of selected variables. The microorganism selected was Bacillus megaterium, a PHA-producing bacterium under conditions of carbon excess and nitrogen limitation. RH was hydrolyzed with formic acid with chloridric acid as enhanced to maximize monosaccharide (glucose, xylose) release and minimize furfural formation, which inhibits bacterial growth. For the growth and PHA-production stages, the influence of hydrolysate concentration in the medium (25, 50, and 75%, v/v) was assessed. Sugar and furfural concentrations were determined by HPLC; bacterial growth was monitored by optical density at 600 nm (OD₆₀₀); and PHAs were quantified by chloroform extraction, sulfuric-acid conversion, and spectrophotometric determination at 240 nm. The greatest PHA concentration (1.78 g PHA per 100 g dry biomass) was obtained using the 120-min hydrolysate diluted to 50%. This lower yield can be attributed to the complexity of the real residue and to inhibitory compounds formed during hydrolysis, such as furfural. In conclusion, rice husk represents a practical substrate for bioplastic synthesis, but further work is required to refine operating conditions and boost PHA yields.
publishDate 2026
dc.date.none.fl_str_mv 2026-05-06T23:16:29Z
2026-08-08
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv V Simposio de Residuos Agropecuarios y Agroindustriales; IX International Symposium on Agricultural and Agroindustrial Waste Management
978-65-88155-15-8
https://simposioderesiduos.com.ar/wp-content/uploads/2026/08/ANAIS-VIII-SIGERA_2025.pdf
https://hdl.handle.net/20.500.12272/14918
identifier_str_mv V Simposio de Residuos Agropecuarios y Agroindustriales; IX International Symposium on Agricultural and Agroindustrial Waste Management
978-65-88155-15-8
url https://simposioderesiduos.com.ar/wp-content/uploads/2026/08/ANAIS-VIII-SIGERA_2025.pdf
https://hdl.handle.net/20.500.12272/14918
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Valorización de residuos lignocelulósicos para la obtención de polímeros biodegradables
PATCRE0010068TC
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
Acceso abierto
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
Acceso abierto
dc.format.none.fl_str_mv pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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