Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression...

Autores: Vaquero, Ana Pena; Calderon Ruiz, Paula; Gambaro, Rocio; Rivero Berti, Ignacio; Limeres, María José; Fraude-El GhazI, Silvia; Huck Iriart, Cristián; Meyer, Claudius U.; Cacicedo, Maximiliano Luis; Hankeln, Thomas; Si, Shutian; Lieberwirth, Ingo; Landfester, Katharina; Alba Soto, Catalina Dirney; Tekiel, Valeria Sonia; Bros, Matthias; Gehring, Stephan; Islan, German Abel
Año de publicación: 2025
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Lipid nanoparticles (LNPs) were engineered for efficient mRNA delivery and immune enhancement through coencapsulation of adjuvants. CpG-oligodeoxynucleotides (CpG-ODN, TLR9 agonist) and monophosphoryl lipid A (MPLA, TLR4 agonist) were incorporated to activate intra- and extracellular Toll-like receptor pathways. Formulated via microfluidics, CpG was added in the aqueous phase and MPLA in the lipid phase. The final LNPMPLA- CpG formulation included Luc mRNA and CpG-ODN (5:1 ratio) with ALC-0315/DSPC/cholesterol/ALC- 0159/MPLA (1 %). Particle characterization by DLS and NTA confirmed neutral, homogeneous nanoparticles (~80 nm). Cryo-TEM and SAXS verified structural integrity. The formulation maintained over 80 % mRNA encapsulation after storage at 4 ◦C and 80 ◦C. Transfection of human and murine dendritic cells (MoDCs and DC2.4) led to robust protein expression. The LNPs showed minimal hemotoxicity and low cytotoxicity, while significantly increasing pro-inflammatory cytokines (IFN-γ, TNF-α, IL-6) in both cell types. DC uptake of LNPMPLA-CpG was efficient. In the in vivo biodistribution, Luc mRNA was primarily expressed in liver and spleenfollowing intramuscular injection. Serum cytokine levels peaked at 6 h post-injection, and flow cytometry of stimulated splenocytes and liver non-parenchymal cells confirmed a strong innate activation. These results support LNP co-delivery of dual adjuvants as a potent platform for enhancing mRNA vaccine efficacy and innate immune activation.
Fil: Vaquero, Ana Pena. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Calderon Ruiz, Paula. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Gambaro, Rocio. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Rivero Berti, Ignacio. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Limeres, María José. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fraude-El GhazI, Silvia. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Huck Iriart, Cristián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Alba - Synchrotron Light Source.; España
Fil: Meyer, Claudius U.. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Cacicedo, Maximiliano Luis. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Hankeln, Thomas. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Si, Shutian. Max Planck Institute For Polymer Research; Alemania
Fil: Lieberwirth, Ingo. Max Planck Institute For Polymer Research; Alemania
Fil: Landfester, Katharina. Max Planck Institute For Polymer Research; Alemania
Fil: Alba Soto, Catalina Dirney. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Bros, Matthias. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Gehring, Stephan. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Islan, German Abel. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Materia: LIPID NANOPARTICLES
mRNA VACCINE
ADJUVANTS
INNATE IMMUNE RESPONSE
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/291539

Acceder

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network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinationsVaquero, Ana PenaCalderon Ruiz, PaulaGambaro, RocioRivero Berti, IgnacioLimeres, María JoséFraude-El GhazI, SilviaHuck Iriart, CristiánMeyer, Claudius U.Cacicedo, Maximiliano LuisHankeln, ThomasSi, ShutianLieberwirth, IngoLandfester, KatharinaAlba Soto, Catalina DirneyTekiel, Valeria SoniaBros, MatthiasGehring, StephanIslan, German AbelLIPID NANOPARTICLESmRNA VACCINEADJUVANTSINNATE IMMUNE RESPONSEhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Lipid nanoparticles (LNPs) were engineered for efficient mRNA delivery and immune enhancement through coencapsulation of adjuvants. CpG-oligodeoxynucleotides (CpG-ODN, TLR9 agonist) and monophosphoryl lipid A (MPLA, TLR4 agonist) were incorporated to activate intra- and extracellular Toll-like receptor pathways. Formulated via microfluidics, CpG was added in the aqueous phase and MPLA in the lipid phase. The final LNPMPLA- CpG formulation included Luc mRNA and CpG-ODN (5:1 ratio) with ALC-0315/DSPC/cholesterol/ALC- 0159/MPLA (1 %). Particle characterization by DLS and NTA confirmed neutral, homogeneous nanoparticles (~80 nm). Cryo-TEM and SAXS verified structural integrity. The formulation maintained over 80 % mRNA encapsulation after storage at 4 ◦C and 80 ◦C. Transfection of human and murine dendritic cells (MoDCs and DC2.4) led to robust protein expression. The LNPs showed minimal hemotoxicity and low cytotoxicity, while significantly increasing pro-inflammatory cytokines (IFN-γ, TNF-α, IL-6) in both cell types. DC uptake of LNPMPLA-CpG was efficient. In the in vivo biodistribution, Luc mRNA was primarily expressed in liver and spleenfollowing intramuscular injection. Serum cytokine levels peaked at 6 h post-injection, and flow cytometry of stimulated splenocytes and liver non-parenchymal cells confirmed a strong innate activation. These results support LNP co-delivery of dual adjuvants as a potent platform for enhancing mRNA vaccine efficacy and innate immune activation.Fil: Vaquero, Ana Pena. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Calderon Ruiz, Paula. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Gambaro, Rocio. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Rivero Berti, Ignacio. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Limeres, María José. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fraude-El GhazI, Silvia. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Huck Iriart, Cristián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Alba - Synchrotron Light Source.; EspañaFil: Meyer, Claudius U.. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Cacicedo, Maximiliano Luis. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Hankeln, Thomas. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Si, Shutian. Max Planck Institute For Polymer Research; AlemaniaFil: Lieberwirth, Ingo. Max Planck Institute For Polymer Research; AlemaniaFil: Landfester, Katharina. Max Planck Institute For Polymer Research; AlemaniaFil: Alba Soto, Catalina Dirney. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Bros, Matthias. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Gehring, Stephan. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Islan, German Abel. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaElsevier Science2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291539Vaquero, Ana Pena; Calderon Ruiz, Paula; Gambaro, Rocio; Rivero Berti, Ignacio; Limeres, María José; et al.; Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations; Elsevier Science; International Journal of Biological Macromolecules; 330; 11-2025; 1-190141-8130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813025086155info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2025.148058info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:54:49Zoai:ri.conicet.gov.ar:11336/291539instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:54:49.368CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations
title	Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations
spellingShingle	Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations Vaquero, Ana Pena LIPID NANOPARTICLES mRNA VACCINE ADJUVANTS INNATE IMMUNE RESPONSE
title_short	Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations
title_full	Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations
title_fullStr	Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations
title_full_unstemmed	Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations
title_sort	Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations
dc.creator.none.fl_str_mv	Vaquero, Ana Pena Calderon Ruiz, Paula Gambaro, Rocio Rivero Berti, Ignacio Limeres, María José Fraude-El GhazI, Silvia Huck Iriart, Cristián Meyer, Claudius U. Cacicedo, Maximiliano Luis Hankeln, Thomas Si, Shutian Lieberwirth, Ingo Landfester, Katharina Alba Soto, Catalina Dirney Tekiel, Valeria Sonia Bros, Matthias Gehring, Stephan Islan, German Abel
author	Vaquero, Ana Pena
author_facet	Vaquero, Ana Pena Calderon Ruiz, Paula Gambaro, Rocio Rivero Berti, Ignacio Limeres, María José Fraude-El GhazI, Silvia Huck Iriart, Cristián Meyer, Claudius U. Cacicedo, Maximiliano Luis Hankeln, Thomas Si, Shutian Lieberwirth, Ingo Landfester, Katharina Alba Soto, Catalina Dirney Tekiel, Valeria Sonia Bros, Matthias Gehring, Stephan Islan, German Abel
author_role	author
author2	Calderon Ruiz, Paula Gambaro, Rocio Rivero Berti, Ignacio Limeres, María José Fraude-El GhazI, Silvia Huck Iriart, Cristián Meyer, Claudius U. Cacicedo, Maximiliano Luis Hankeln, Thomas Si, Shutian Lieberwirth, Ingo Landfester, Katharina Alba Soto, Catalina Dirney Tekiel, Valeria Sonia Bros, Matthias Gehring, Stephan Islan, German Abel
author2_role	author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	LIPID NANOPARTICLES mRNA VACCINE ADJUVANTS INNATE IMMUNE RESPONSE
topic	LIPID NANOPARTICLES mRNA VACCINE ADJUVANTS INNATE IMMUNE RESPONSE
purl_subject.fl_str_mv	https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv	Lipid nanoparticles (LNPs) were engineered for efficient mRNA delivery and immune enhancement through coencapsulation of adjuvants. CpG-oligodeoxynucleotides (CpG-ODN, TLR9 agonist) and monophosphoryl lipid A (MPLA, TLR4 agonist) were incorporated to activate intra- and extracellular Toll-like receptor pathways. Formulated via microfluidics, CpG was added in the aqueous phase and MPLA in the lipid phase. The final LNPMPLA- CpG formulation included Luc mRNA and CpG-ODN (5:1 ratio) with ALC-0315/DSPC/cholesterol/ALC- 0159/MPLA (1 %). Particle characterization by DLS and NTA confirmed neutral, homogeneous nanoparticles (~80 nm). Cryo-TEM and SAXS verified structural integrity. The formulation maintained over 80 % mRNA encapsulation after storage at 4 ◦C and 80 ◦C. Transfection of human and murine dendritic cells (MoDCs and DC2.4) led to robust protein expression. The LNPs showed minimal hemotoxicity and low cytotoxicity, while significantly increasing pro-inflammatory cytokines (IFN-γ, TNF-α, IL-6) in both cell types. DC uptake of LNPMPLA-CpG was efficient. In the in vivo biodistribution, Luc mRNA was primarily expressed in liver and spleenfollowing intramuscular injection. Serum cytokine levels peaked at 6 h post-injection, and flow cytometry of stimulated splenocytes and liver non-parenchymal cells confirmed a strong innate activation. These results support LNP co-delivery of dual adjuvants as a potent platform for enhancing mRNA vaccine efficacy and innate immune activation. Fil: Vaquero, Ana Pena. Johannes Gutenberg Universitat Mainz; Alemania Fil: Calderon Ruiz, Paula. Johannes Gutenberg Universitat Mainz; Alemania Fil: Gambaro, Rocio. Johannes Gutenberg Universitat Mainz; Alemania Fil: Rivero Berti, Ignacio. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Limeres, María José. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fraude-El GhazI, Silvia. Johannes Gutenberg Universitat Mainz; Alemania Fil: Huck Iriart, Cristián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Alba - Synchrotron Light Source.; España Fil: Meyer, Claudius U.. Johannes Gutenberg Universitat Mainz; Alemania Fil: Cacicedo, Maximiliano Luis. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Hankeln, Thomas. Johannes Gutenberg Universitat Mainz; Alemania Fil: Si, Shutian. Max Planck Institute For Polymer Research; Alemania Fil: Lieberwirth, Ingo. Max Planck Institute For Polymer Research; Alemania Fil: Landfester, Katharina. Max Planck Institute For Polymer Research; Alemania Fil: Alba Soto, Catalina Dirney. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Bros, Matthias. Johannes Gutenberg Universitat Mainz; Alemania Fil: Gehring, Stephan. Johannes Gutenberg Universitat Mainz; Alemania Fil: Islan, German Abel. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description	Lipid nanoparticles (LNPs) were engineered for efficient mRNA delivery and immune enhancement through coencapsulation of adjuvants. CpG-oligodeoxynucleotides (CpG-ODN, TLR9 agonist) and monophosphoryl lipid A (MPLA, TLR4 agonist) were incorporated to activate intra- and extracellular Toll-like receptor pathways. Formulated via microfluidics, CpG was added in the aqueous phase and MPLA in the lipid phase. The final LNPMPLA- CpG formulation included Luc mRNA and CpG-ODN (5:1 ratio) with ALC-0315/DSPC/cholesterol/ALC- 0159/MPLA (1 %). Particle characterization by DLS and NTA confirmed neutral, homogeneous nanoparticles (~80 nm). Cryo-TEM and SAXS verified structural integrity. The formulation maintained over 80 % mRNA encapsulation after storage at 4 ◦C and 80 ◦C. Transfection of human and murine dendritic cells (MoDCs and DC2.4) led to robust protein expression. The LNPs showed minimal hemotoxicity and low cytotoxicity, while significantly increasing pro-inflammatory cytokines (IFN-γ, TNF-α, IL-6) in both cell types. DC uptake of LNPMPLA-CpG was efficient. In the in vivo biodistribution, Luc mRNA was primarily expressed in liver and spleenfollowing intramuscular injection. Serum cytokine levels peaked at 6 h post-injection, and flow cytometry of stimulated splenocytes and liver non-parenchymal cells confirmed a strong innate activation. These results support LNP co-delivery of dual adjuvants as a potent platform for enhancing mRNA vaccine efficacy and innate immune activation.
publishDate	2025
dc.date.none.fl_str_mv	2025-11
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/291539 Vaquero, Ana Pena; Calderon Ruiz, Paula; Gambaro, Rocio; Rivero Berti, Ignacio; Limeres, María José; et al.; Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations; Elsevier Science; International Journal of Biological Macromolecules; 330; 11-2025; 1-19 0141-8130 CONICET Digital CONICET
url	http://hdl.handle.net/11336/291539
identifier_str_mv	Vaquero, Ana Pena; Calderon Ruiz, Paula; Gambaro, Rocio; Rivero Berti, Ignacio; Limeres, María José; et al.; Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression for mRNA-based vaccinations; Elsevier Science; International Journal of Biological Macromolecules; 330; 11-2025; 1-19 0141-8130 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813025086155 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2025.148058
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Elsevier Science
publisher.none.fl_str_mv	Elsevier Science
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	13.24418

Incorporation of monophosphoryl lipid A and CpG-oligodeoxynucleotides into lipid nanoparticles activates toll-like receptor signaling pathways while maintaining antigen expression...

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