Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina

Autores
Molina, Nora Beatriz; León, Laura Beltina; Grenóvero, Silvia; Bosch, María Alejandra; López, Marisa; Sparo, Mónica Delfina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biofilm formation by Escherichia coli contributes to the persistence of infections. In order to deepen the knowledge about the pathogenesis of E. coli infections, the objective was to assess the biofilm production and the expression of virulence factors of human clinical isolates of E. coli from enteric and urinary infections from Argentina. We analyzed 105 isolates of diarrheagenic E. coli (DEC) and uropathogenic E. coli (UPEC). All isolates (except one) were biofilm producers. The moderate biofilm former (MBF) category was prevalent (38.1%). Curli fimbriae was the most highly expressed virulence factor (94.3%), whereas cellulose, hemolysins, and hemagglutinins were detected at lower frequencies (43.8%, 35.2%, and 33.3%), respectively. The number of DEC isolates that express cellulose was significantly higher than the number of UPEC isolates (p<0 01). The multifactorial nature of biofilm of E. coli indicates the need for new approaches to understand the pathogenesis and persistence of biofilm-related infections.
Fil: Molina, Nora Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro Universitario de Estudios Microbiológicos y Parasitológicos; Argentina
Fil: León, Laura Beltina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Grenóvero, Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro Universitario de Estudios Microbiológicos y Parasitológicos; Argentina
Fil: Bosch, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: López, Marisa. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro Universitario de Estudios Microbiológicos y Parasitológicos; Argentina
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Materia
BIOFILMS
CELLULOSE
CURLI
DEC
HUMAN CLINICAL ISOLATES
UPEC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291637
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291637
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From ArgentinaMolina, Nora BeatrizLeón, Laura BeltinaGrenóvero, SilviaBosch, María AlejandraLópez, MarisaSparo, Mónica DelfinaBIOFILMSCELLULOSECURLIDECHUMAN CLINICAL ISOLATESUPEChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Biofilm formation by Escherichia coli contributes to the persistence of infections. In order to deepen the knowledge about the pathogenesis of E. coli infections, the objective was to assess the biofilm production and the expression of virulence factors of human clinical isolates of E. coli from enteric and urinary infections from Argentina. We analyzed 105 isolates of diarrheagenic E. coli (DEC) and uropathogenic E. coli (UPEC). All isolates (except one) were biofilm producers. The moderate biofilm former (MBF) category was prevalent (38.1%). Curli fimbriae was the most highly expressed virulence factor (94.3%), whereas cellulose, hemolysins, and hemagglutinins were detected at lower frequencies (43.8%, 35.2%, and 33.3%), respectively. The number of DEC isolates that express cellulose was significantly higher than the number of UPEC isolates (p<0 01). The multifactorial nature of biofilm of E. coli indicates the need for new approaches to understand the pathogenesis and persistence of biofilm-related infections.Fil: Molina, Nora Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro Universitario de Estudios Microbiológicos y Parasitológicos; ArgentinaFil: León, Laura Beltina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Grenóvero, Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro Universitario de Estudios Microbiológicos y Parasitológicos; ArgentinaFil: Bosch, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: López, Marisa. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro Universitario de Estudios Microbiológicos y Parasitológicos; ArgentinaFil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaJohn Wiley & Sons2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291637Molina, Nora Beatriz; León, Laura Beltina; Grenóvero, Silvia; Bosch, María Alejandra; López, Marisa; et al.; Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina; John Wiley & Sons; Advanced Gut & Microbiome Research; 2025; 1; 2-2025; 1-82755-1652CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1155/agm3/3243778info:eu-repo/semantics/altIdentifier/doi/10.1155/agm3/3243778info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:48:59Zoai:ri.conicet.gov.ar:11336/291637instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:48:59.954CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina
title Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina
spellingShingle Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina
Molina, Nora Beatriz
BIOFILMS
CELLULOSE
CURLI
DEC
HUMAN CLINICAL ISOLATES
UPEC
title_short Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina
title_full Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina
title_fullStr Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina
title_full_unstemmed Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina
title_sort Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina
dc.creator.none.fl_str_mv Molina, Nora Beatriz
León, Laura Beltina
Grenóvero, Silvia
Bosch, María Alejandra
López, Marisa
Sparo, Mónica Delfina
author Molina, Nora Beatriz
author_facet Molina, Nora Beatriz
León, Laura Beltina
Grenóvero, Silvia
Bosch, María Alejandra
López, Marisa
Sparo, Mónica Delfina
author_role author
author2 León, Laura Beltina
Grenóvero, Silvia
Bosch, María Alejandra
López, Marisa
Sparo, Mónica Delfina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOFILMS
CELLULOSE
CURLI
DEC
HUMAN CLINICAL ISOLATES
UPEC
topic BIOFILMS
CELLULOSE
CURLI
DEC
HUMAN CLINICAL ISOLATES
UPEC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biofilm formation by Escherichia coli contributes to the persistence of infections. In order to deepen the knowledge about the pathogenesis of E. coli infections, the objective was to assess the biofilm production and the expression of virulence factors of human clinical isolates of E. coli from enteric and urinary infections from Argentina. We analyzed 105 isolates of diarrheagenic E. coli (DEC) and uropathogenic E. coli (UPEC). All isolates (except one) were biofilm producers. The moderate biofilm former (MBF) category was prevalent (38.1%). Curli fimbriae was the most highly expressed virulence factor (94.3%), whereas cellulose, hemolysins, and hemagglutinins were detected at lower frequencies (43.8%, 35.2%, and 33.3%), respectively. The number of DEC isolates that express cellulose was significantly higher than the number of UPEC isolates (p<0 01). The multifactorial nature of biofilm of E. coli indicates the need for new approaches to understand the pathogenesis and persistence of biofilm-related infections.
Fil: Molina, Nora Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro Universitario de Estudios Microbiológicos y Parasitológicos; Argentina
Fil: León, Laura Beltina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Grenóvero, Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro Universitario de Estudios Microbiológicos y Parasitológicos; Argentina
Fil: Bosch, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: López, Marisa. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro Universitario de Estudios Microbiológicos y Parasitológicos; Argentina
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
description Biofilm formation by Escherichia coli contributes to the persistence of infections. In order to deepen the knowledge about the pathogenesis of E. coli infections, the objective was to assess the biofilm production and the expression of virulence factors of human clinical isolates of E. coli from enteric and urinary infections from Argentina. We analyzed 105 isolates of diarrheagenic E. coli (DEC) and uropathogenic E. coli (UPEC). All isolates (except one) were biofilm producers. The moderate biofilm former (MBF) category was prevalent (38.1%). Curli fimbriae was the most highly expressed virulence factor (94.3%), whereas cellulose, hemolysins, and hemagglutinins were detected at lower frequencies (43.8%, 35.2%, and 33.3%), respectively. The number of DEC isolates that express cellulose was significantly higher than the number of UPEC isolates (p<0 01). The multifactorial nature of biofilm of E. coli indicates the need for new approaches to understand the pathogenesis and persistence of biofilm-related infections.
publishDate 2025
dc.date.none.fl_str_mv 2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291637
Molina, Nora Beatriz; León, Laura Beltina; Grenóvero, Silvia; Bosch, María Alejandra; López, Marisa; et al.; Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina; John Wiley & Sons; Advanced Gut & Microbiome Research; 2025; 1; 2-2025; 1-8
2755-1652
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291637
identifier_str_mv Molina, Nora Beatriz; León, Laura Beltina; Grenóvero, Silvia; Bosch, María Alejandra; López, Marisa; et al.; Diarrheagenic and Uropathogenic Escherichia coli : Biofilm Production and Virulence Factors in Clinical Isolates From Argentina; John Wiley & Sons; Advanced Gut & Microbiome Research; 2025; 1; 2-2025; 1-8
2755-1652
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1155/agm3/3243778
info:eu-repo/semantics/altIdentifier/doi/10.1155/agm3/3243778
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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