Assessment of development and reproductive toxicology (DART): an in vitro approach

Autores
Gulin, Julián Ernesto Nicolás; Lorenzo, María Soledad; Maruri, Alejandro; Torres, Pablo; Lombardo, Daniel Marcelo
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Reproductive toxicity can be studied by a holistic approach on experimental animals in extensive and expensive trials. However, using an integrated in vitro approach, the reproductive cycle can be splitinto its main biological components integrating results and providing comprehensive information on the potential effects of chemicals on gametes and embryonic development, decreasing the number ofanimal studies and providing a more detailed toxicological profile. This work aims to introduce the setting up of an in vitro platform for DART testing, applying a battery of assays based on standardizedreproductive biotechnologies. To evaluate the toxicity on male gametes, bovine spermatozoa were exposed to increasing concentrations of the test substance, studying viability with vital stains alongwith motility and velocity parameters assessed by computer-assisted sperm analysis (CASA). The bovine oocyte in vitro (bIVM) was applied to assess the effect of substances during oocyte maturation.Cumulus-oocyte complexes (COCs) obtained from ovaries of slaughter cows were selected and incubated in IVM medium with increasing compound concentrations. After 22 h, the viability andnuclear maturation were evaluated. The obtaining results allowed determining the concentration that reduced the IVM of oocytes by 50% compared to the untreated control. In vitro fertilization of bovineoocyte (bFIV) and early embryonic development were used as a model to evaluate the toxic effect of the fertilization process. Bovine gametes were co-incubated with increasing concentrations of thetest substance. After 18 h, pronucleus formation was registered to determine the concentration that reduced the bFIV by 50% compared to the untreated control. This initial battery of in vitro modelsis part of developing a biotechnological platform for DART testing, promoting the technological cooperation between academia and industry partners within a 3Rs approach.
Fil: Gulin, Julián Ernesto Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Lorenzo, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Maruri, Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torres, Pablo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
Reproductive Toxicology
In vitro
DART
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291593
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291593
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessment of development and reproductive toxicology (DART): an in vitro approachGulin, Julián Ernesto NicolásLorenzo, María SoledadMaruri, AlejandroTorres, PabloLombardo, Daniel MarceloReproductive ToxicologyIn vitroDARThttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Reproductive toxicity can be studied by a holistic approach on experimental animals in extensive and expensive trials. However, using an integrated in vitro approach, the reproductive cycle can be splitinto its main biological components integrating results and providing comprehensive information on the potential effects of chemicals on gametes and embryonic development, decreasing the number ofanimal studies and providing a more detailed toxicological profile. This work aims to introduce the setting up of an in vitro platform for DART testing, applying a battery of assays based on standardizedreproductive biotechnologies. To evaluate the toxicity on male gametes, bovine spermatozoa were exposed to increasing concentrations of the test substance, studying viability with vital stains alongwith motility and velocity parameters assessed by computer-assisted sperm analysis (CASA). The bovine oocyte in vitro (bIVM) was applied to assess the effect of substances during oocyte maturation.Cumulus-oocyte complexes (COCs) obtained from ovaries of slaughter cows were selected and incubated in IVM medium with increasing compound concentrations. After 22 h, the viability andnuclear maturation were evaluated. The obtaining results allowed determining the concentration that reduced the IVM of oocytes by 50% compared to the untreated control. In vitro fertilization of bovineoocyte (bFIV) and early embryonic development were used as a model to evaluate the toxic effect of the fertilization process. Bovine gametes were co-incubated with increasing concentrations of thetest substance. After 18 h, pronucleus formation was registered to determine the concentration that reduced the bFIV by 50% compared to the untreated control. This initial battery of in vitro modelsis part of developing a biotechnological platform for DART testing, promoting the technological cooperation between academia and industry partners within a 3Rs approach.Fil: Gulin, Julián Ernesto Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Lorenzo, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Maruri, Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Torres, Pablo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaLXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291593Assessment of development and reproductive toxicology (DART): an in vitro approach; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 1-10025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol83-23/s5/1s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:44:19Zoai:ri.conicet.gov.ar:11336/291593instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:44:19.694CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of development and reproductive toxicology (DART): an in vitro approach
title Assessment of development and reproductive toxicology (DART): an in vitro approach
spellingShingle Assessment of development and reproductive toxicology (DART): an in vitro approach
Gulin, Julián Ernesto Nicolás
Reproductive Toxicology
In vitro
DART
title_short Assessment of development and reproductive toxicology (DART): an in vitro approach
title_full Assessment of development and reproductive toxicology (DART): an in vitro approach
title_fullStr Assessment of development and reproductive toxicology (DART): an in vitro approach
title_full_unstemmed Assessment of development and reproductive toxicology (DART): an in vitro approach
title_sort Assessment of development and reproductive toxicology (DART): an in vitro approach
dc.creator.none.fl_str_mv Gulin, Julián Ernesto Nicolás
Lorenzo, María Soledad
Maruri, Alejandro
Torres, Pablo
Lombardo, Daniel Marcelo
author Gulin, Julián Ernesto Nicolás
author_facet Gulin, Julián Ernesto Nicolás
Lorenzo, María Soledad
Maruri, Alejandro
Torres, Pablo
Lombardo, Daniel Marcelo
author_role author
author2 Lorenzo, María Soledad
Maruri, Alejandro
Torres, Pablo
Lombardo, Daniel Marcelo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Reproductive Toxicology
In vitro
DART
topic Reproductive Toxicology
In vitro
DART
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Reproductive toxicity can be studied by a holistic approach on experimental animals in extensive and expensive trials. However, using an integrated in vitro approach, the reproductive cycle can be splitinto its main biological components integrating results and providing comprehensive information on the potential effects of chemicals on gametes and embryonic development, decreasing the number ofanimal studies and providing a more detailed toxicological profile. This work aims to introduce the setting up of an in vitro platform for DART testing, applying a battery of assays based on standardizedreproductive biotechnologies. To evaluate the toxicity on male gametes, bovine spermatozoa were exposed to increasing concentrations of the test substance, studying viability with vital stains alongwith motility and velocity parameters assessed by computer-assisted sperm analysis (CASA). The bovine oocyte in vitro (bIVM) was applied to assess the effect of substances during oocyte maturation.Cumulus-oocyte complexes (COCs) obtained from ovaries of slaughter cows were selected and incubated in IVM medium with increasing compound concentrations. After 22 h, the viability andnuclear maturation were evaluated. The obtaining results allowed determining the concentration that reduced the IVM of oocytes by 50% compared to the untreated control. In vitro fertilization of bovineoocyte (bFIV) and early embryonic development were used as a model to evaluate the toxic effect of the fertilization process. Bovine gametes were co-incubated with increasing concentrations of thetest substance. After 18 h, pronucleus formation was registered to determine the concentration that reduced the bFIV by 50% compared to the untreated control. This initial battery of in vitro modelsis part of developing a biotechnological platform for DART testing, promoting the technological cooperation between academia and industry partners within a 3Rs approach.
Fil: Gulin, Julián Ernesto Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Lorenzo, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Maruri, Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torres, Pablo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Reproductive toxicity can be studied by a holistic approach on experimental animals in extensive and expensive trials. However, using an integrated in vitro approach, the reproductive cycle can be splitinto its main biological components integrating results and providing comprehensive information on the potential effects of chemicals on gametes and embryonic development, decreasing the number ofanimal studies and providing a more detailed toxicological profile. This work aims to introduce the setting up of an in vitro platform for DART testing, applying a battery of assays based on standardizedreproductive biotechnologies. To evaluate the toxicity on male gametes, bovine spermatozoa were exposed to increasing concentrations of the test substance, studying viability with vital stains alongwith motility and velocity parameters assessed by computer-assisted sperm analysis (CASA). The bovine oocyte in vitro (bIVM) was applied to assess the effect of substances during oocyte maturation.Cumulus-oocyte complexes (COCs) obtained from ovaries of slaughter cows were selected and incubated in IVM medium with increasing compound concentrations. After 22 h, the viability andnuclear maturation were evaluated. The obtaining results allowed determining the concentration that reduced the IVM of oocytes by 50% compared to the untreated control. In vitro fertilization of bovineoocyte (bFIV) and early embryonic development were used as a model to evaluate the toxic effect of the fertilization process. Bovine gametes were co-incubated with increasing concentrations of thetest substance. After 18 h, pronucleus formation was registered to determine the concentration that reduced the bFIV by 50% compared to the untreated control. This initial battery of in vitro modelsis part of developing a biotechnological platform for DART testing, promoting the technological cooperation between academia and industry partners within a 3Rs approach.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291593
Assessment of development and reproductive toxicology (DART): an in vitro approach; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 1-1
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291593
identifier_str_mv Assessment of development and reproductive toxicology (DART): an in vitro approach; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 1-1
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol83-23/s5/1s5.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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