Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease

Autores
Larsen, Alejandra; González, Ester Teresa; Serena, Maria Soledad; Echeverria, Maria Gabriela; Mortola, Eduardo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
Fil: Larsen, Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: González, Ester Teresa. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Serena, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Mortola, Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Materia
Bovine Leukemia Virus
Immunodetection
Baculovirus System
Recombinant P24 Protein
Immunoblotting Assay
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11513
Acceder
id CONICETDig_90e66429f3a86ea3783ea620f0594894
oai_identifier_str oai:ri.conicet.gov.ar:11336/11513
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the diseaseLarsen, AlejandraGonzález, Ester TeresaSerena, Maria SoledadEcheverria, Maria GabrielaMortola, EduardoBovine Leukemia VirusImmunodetectionBaculovirus SystemRecombinant P24 ProteinImmunoblotting Assayhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.Fil: Larsen, Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: González, Ester Teresa. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Serena, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Mortola, Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaHumana Press2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11513Larsen, Alejandra; González, Ester Teresa; Serena, Maria Soledad; Echeverria, Maria Gabriela; Mortola, Eduardo; Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease; Humana Press; Molecular Biotechnology; 54; 2; 6-2013; 475–4831073-60851559-0305enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12033-012-9587-7info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs12033-012-9587-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:41:35Zoai:ri.conicet.gov.ar:11336/11513instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:41:35.777CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease
title Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease
spellingShingle Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease
Larsen, Alejandra
Bovine Leukemia Virus
Immunodetection
Baculovirus System
Recombinant P24 Protein
Immunoblotting Assay
title_short Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease
title_full Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease
title_fullStr Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease
title_full_unstemmed Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease
title_sort Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease
dc.creator.none.fl_str_mv Larsen, Alejandra
González, Ester Teresa
Serena, Maria Soledad
Echeverria, Maria Gabriela
Mortola, Eduardo
author Larsen, Alejandra
author_facet Larsen, Alejandra
González, Ester Teresa
Serena, Maria Soledad
Echeverria, Maria Gabriela
Mortola, Eduardo
author_role author
author2 González, Ester Teresa
Serena, Maria Soledad
Echeverria, Maria Gabriela
Mortola, Eduardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bovine Leukemia Virus
Immunodetection
Baculovirus System
Recombinant P24 Protein
Immunoblotting Assay
topic Bovine Leukemia Virus
Immunodetection
Baculovirus System
Recombinant P24 Protein
Immunoblotting Assay
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
Fil: Larsen, Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: González, Ester Teresa. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Serena, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Mortola, Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11513
Larsen, Alejandra; González, Ester Teresa; Serena, Maria Soledad; Echeverria, Maria Gabriela; Mortola, Eduardo; Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease; Humana Press; Molecular Biotechnology; 54; 2; 6-2013; 475–483
1073-6085
1559-0305
url http://hdl.handle.net/11336/11513
identifier_str_mv Larsen, Alejandra; González, Ester Teresa; Serena, Maria Soledad; Echeverria, Maria Gabriela; Mortola, Eduardo; Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease; Humana Press; Molecular Biotechnology; 54; 2; 6-2013; 475–483
1073-6085
1559-0305
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s12033-012-9587-7
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs12033-012-9587-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1874774484985053184
score 13.365483

Publicaciones similares