Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease

Autores
Larsen, Alejandra Edith; González, Ester Teresa; Serena, María Soledad; Echeverría, María Gabriela; Mórtola, Eduardo Carlos
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
Facultad de Ciencias Veterinarias
Materia
Veterinaria
Bovine leukemia virus
Immunodetection
Baculovirus system
Recombinant p24 protein
Immunoblotting assay
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/136518
Acceder
id SEDICI_542240d5743746ae8be0fc0666440c44
oai_identifier_str oai:sedici.unlp.edu.ar:10915/136518
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the DiseaseLarsen, Alejandra EdithGonzález, Ester TeresaSerena, María SoledadEcheverría, María GabrielaMórtola, Eduardo CarlosVeterinariaBovine leukemia virusImmunodetectionBaculovirus systemRecombinant p24 proteinImmunoblotting assayBovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.Facultad de Ciencias Veterinarias2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf475-483http://sedici.unlp.edu.ar/handle/10915/136518enginfo:eu-repo/semantics/altIdentifier/issn/1559-0305info:eu-repo/semantics/altIdentifier/issn/1073-6085info:eu-repo/semantics/altIdentifier/doi/10.1007/s12033-012-9587-7info:eu-repo/semantics/altIdentifier/pmid/22829115info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2026-06-23T10:54:35Zoai:sedici.unlp.edu.ar:10915/136518Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292026-06-23 10:54:36.043SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
spellingShingle Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
Larsen, Alejandra Edith
Veterinaria
Bovine leukemia virus
Immunodetection
Baculovirus system
Recombinant p24 protein
Immunoblotting assay
title_short Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title_full Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title_fullStr Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title_full_unstemmed Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title_sort Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
dc.creator.none.fl_str_mv Larsen, Alejandra Edith
González, Ester Teresa
Serena, María Soledad
Echeverría, María Gabriela
Mórtola, Eduardo Carlos
author Larsen, Alejandra Edith
author_facet Larsen, Alejandra Edith
González, Ester Teresa
Serena, María Soledad
Echeverría, María Gabriela
Mórtola, Eduardo Carlos
author_role author
author2 González, Ester Teresa
Serena, María Soledad
Echeverría, María Gabriela
Mórtola, Eduardo Carlos
author2_role author
author
author
author
dc.subject.none.fl_str_mv Veterinaria
Bovine leukemia virus
Immunodetection
Baculovirus system
Recombinant p24 protein
Immunoblotting assay
topic Veterinaria
Bovine leukemia virus
Immunodetection
Baculovirus system
Recombinant p24 protein
Immunoblotting assay
dc.description.none.fl_txt_mv Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
Facultad de Ciencias Veterinarias
description Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/136518
url http://sedici.unlp.edu.ar/handle/10915/136518
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1559-0305
info:eu-repo/semantics/altIdentifier/issn/1073-6085
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12033-012-9587-7
info:eu-repo/semantics/altIdentifier/pmid/22829115
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
475-483
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.365483

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