Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels

Autores
Somoza, Sofía Cristina; Boccardo, Noelia Ayelen; Santin, Franco; Sede, Ana Rocío; Wengier, Diego Leonardo; Boissonnet, Carlos Pablo; Muschietti, Jorge Prometeo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In recent years, the rapid alkalinization factor (RALF) family of cysteine-rich peptides has been reported to be crucial for several plant signaling mechanisms, including cell growth, plant immunity and fertilization. RALF4 and RALF19 (RALF4/19) pollen peptides redundantly regulate the pollen tube integrity and growth through binding to their receptors ANXUR1/2 (ANX1/2) and Buddha’s Paper Seal 1 and 2 (BUPS1/2), members of the Catharanthus roseus RLK1-like (CrRLK1L) family, and, thus, are essential for plant fertilization. However, the signaling mechanisms at the cellular level that follow these binding events remain unclear. In this study, we show that the addition of synthetic peptide RALF4 rapidly halts pollen tube growth along with the excessive deposition of plasma membrane and cell wall material at the tip. The ratiometric imaging of genetically encoded ROS and Ca2+ sensors-expressing pollen tubes shows that RALF4 treatment modulates the cytoplasmic levels of reactive oxygen species (ROS) and calcium (Ca2+) in opposite ways at the tip. Thus, we propose that pollen RALF4/19 peptides bind ANX1/2 and BUPS1/2 to regulate ROS and calcium homeostasis to ensure proper cell wall integrity and control of pollen tube growth.
Fil: Somoza, Sofía Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Boccardo, Noelia Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Santin, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Sede, Ana Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Boissonnet, Carlos Pablo. University of Cologne; Alemania
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
pollen
ralf peptides
calcium
ROS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260810
Acceder
id CONICETDig_a4ff50e2b243e11fd85c8f498ad3537c
oai_identifier_str oai:ri.conicet.gov.ar:11336/260810
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip LevelsSomoza, Sofía CristinaBoccardo, Noelia AyelenSantin, FrancoSede, Ana RocíoWengier, Diego LeonardoBoissonnet, Carlos PabloMuschietti, Jorge Prometeopollenralf peptidescalciumROShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In recent years, the rapid alkalinization factor (RALF) family of cysteine-rich peptides has been reported to be crucial for several plant signaling mechanisms, including cell growth, plant immunity and fertilization. RALF4 and RALF19 (RALF4/19) pollen peptides redundantly regulate the pollen tube integrity and growth through binding to their receptors ANXUR1/2 (ANX1/2) and Buddha’s Paper Seal 1 and 2 (BUPS1/2), members of the Catharanthus roseus RLK1-like (CrRLK1L) family, and, thus, are essential for plant fertilization. However, the signaling mechanisms at the cellular level that follow these binding events remain unclear. In this study, we show that the addition of synthetic peptide RALF4 rapidly halts pollen tube growth along with the excessive deposition of plasma membrane and cell wall material at the tip. The ratiometric imaging of genetically encoded ROS and Ca2+ sensors-expressing pollen tubes shows that RALF4 treatment modulates the cytoplasmic levels of reactive oxygen species (ROS) and calcium (Ca2+) in opposite ways at the tip. Thus, we propose that pollen RALF4/19 peptides bind ANX1/2 and BUPS1/2 to regulate ROS and calcium homeostasis to ensure proper cell wall integrity and control of pollen tube growth.Fil: Somoza, Sofía Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Boccardo, Noelia Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Santin, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Sede, Ana Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Boissonnet, Carlos Pablo. University of Cologne; AlemaniaFil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaMDPI2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260810Somoza, Sofía Cristina; Boccardo, Noelia Ayelen; Santin, Franco; Sede, Ana Rocío; Wengier, Diego Leonardo; et al.; Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels; MDPI; Biomolecules; 14; 11; 10-2024; 1-162218-273XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-273X/14/11/1375info:eu-repo/semantics/altIdentifier/doi/10.3390/biom14111375info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:42:47Zoai:ri.conicet.gov.ar:11336/260810instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:42:47.798CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels
title Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels
spellingShingle Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels
Somoza, Sofía Cristina
pollen
ralf peptides
calcium
ROS
title_short Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels
title_full Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels
title_fullStr Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels
title_full_unstemmed Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels
title_sort Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels
dc.creator.none.fl_str_mv Somoza, Sofía Cristina
Boccardo, Noelia Ayelen
Santin, Franco
Sede, Ana Rocío
Wengier, Diego Leonardo
Boissonnet, Carlos Pablo
Muschietti, Jorge Prometeo
author Somoza, Sofía Cristina
author_facet Somoza, Sofía Cristina
Boccardo, Noelia Ayelen
Santin, Franco
Sede, Ana Rocío
Wengier, Diego Leonardo
Boissonnet, Carlos Pablo
Muschietti, Jorge Prometeo
author_role author
author2 Boccardo, Noelia Ayelen
Santin, Franco
Sede, Ana Rocío
Wengier, Diego Leonardo
Boissonnet, Carlos Pablo
Muschietti, Jorge Prometeo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv pollen
ralf peptides
calcium
ROS
topic pollen
ralf peptides
calcium
ROS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In recent years, the rapid alkalinization factor (RALF) family of cysteine-rich peptides has been reported to be crucial for several plant signaling mechanisms, including cell growth, plant immunity and fertilization. RALF4 and RALF19 (RALF4/19) pollen peptides redundantly regulate the pollen tube integrity and growth through binding to their receptors ANXUR1/2 (ANX1/2) and Buddha’s Paper Seal 1 and 2 (BUPS1/2), members of the Catharanthus roseus RLK1-like (CrRLK1L) family, and, thus, are essential for plant fertilization. However, the signaling mechanisms at the cellular level that follow these binding events remain unclear. In this study, we show that the addition of synthetic peptide RALF4 rapidly halts pollen tube growth along with the excessive deposition of plasma membrane and cell wall material at the tip. The ratiometric imaging of genetically encoded ROS and Ca2+ sensors-expressing pollen tubes shows that RALF4 treatment modulates the cytoplasmic levels of reactive oxygen species (ROS) and calcium (Ca2+) in opposite ways at the tip. Thus, we propose that pollen RALF4/19 peptides bind ANX1/2 and BUPS1/2 to regulate ROS and calcium homeostasis to ensure proper cell wall integrity and control of pollen tube growth.
Fil: Somoza, Sofía Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Boccardo, Noelia Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Santin, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Sede, Ana Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Boissonnet, Carlos Pablo. University of Cologne; Alemania
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description In recent years, the rapid alkalinization factor (RALF) family of cysteine-rich peptides has been reported to be crucial for several plant signaling mechanisms, including cell growth, plant immunity and fertilization. RALF4 and RALF19 (RALF4/19) pollen peptides redundantly regulate the pollen tube integrity and growth through binding to their receptors ANXUR1/2 (ANX1/2) and Buddha’s Paper Seal 1 and 2 (BUPS1/2), members of the Catharanthus roseus RLK1-like (CrRLK1L) family, and, thus, are essential for plant fertilization. However, the signaling mechanisms at the cellular level that follow these binding events remain unclear. In this study, we show that the addition of synthetic peptide RALF4 rapidly halts pollen tube growth along with the excessive deposition of plasma membrane and cell wall material at the tip. The ratiometric imaging of genetically encoded ROS and Ca2+ sensors-expressing pollen tubes shows that RALF4 treatment modulates the cytoplasmic levels of reactive oxygen species (ROS) and calcium (Ca2+) in opposite ways at the tip. Thus, we propose that pollen RALF4/19 peptides bind ANX1/2 and BUPS1/2 to regulate ROS and calcium homeostasis to ensure proper cell wall integrity and control of pollen tube growth.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260810
Somoza, Sofía Cristina; Boccardo, Noelia Ayelen; Santin, Franco; Sede, Ana Rocío; Wengier, Diego Leonardo; et al.; Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels; MDPI; Biomolecules; 14; 11; 10-2024; 1-16
2218-273X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260810
identifier_str_mv Somoza, Sofía Cristina; Boccardo, Noelia Ayelen; Santin, Franco; Sede, Ana Rocío; Wengier, Diego Leonardo; et al.; Arabidopsis RALF4 Rapidly Halts Pollen Tube Growth by Increasing ROS and Decreasing Calcium Cytoplasmic Tip Levels; MDPI; Biomolecules; 14; 11; 10-2024; 1-16
2218-273X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-273X/14/11/1375
info:eu-repo/semantics/altIdentifier/doi/10.3390/biom14111375
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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